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The map label for this gene is nuoB1 [H]

Identifier: 222526369

GI number: 222526369

Start: 3878748

End: 3879272

Strand: Direct

Name: nuoB1 [H]

Synonym: Chy400_3135

Alternate gene names: 222526369

Gene position: 3878748-3879272 (Clockwise)

Preceding gene: 222526368

Following gene: 222526370

Centisome position: 73.62

GC content: 56.0

Gene sequence:

>525_bases
ATGGGAATAGAGGAGAAGGCTGGTAATCTTGGGATTGTGACTACAACGCTCGAGACGGTTGTTAATTGGGGCCGGACAAA
TGCGATGTGGCCCTTGCTTTTTGGTCTAGCTTGCTGTGCCATCGAGATGATGGGCGCGCAGGCGAGCAATTACGACCTCT
CGCGCTTTGGCATGGAATTGAACCGCGCTTCGCCCCGCCAGGCCGATCTGATGATTGTTGCCGGGCGTGTGTCGCGGAAG
ATGGCGCCGGTTGTGCGTCGCCTGTACGATCAGATGCCCGAACCCAAGTGGGTGATTGCGATGGGTGATTGCGCCGCCTG
TGGTGGTATCTTTAACAACTACGCCATCGTTCAGGGGGTTGATGAGGTGGTTCCGGTTGATGTCTATGTCGCCGGTTGTC
CGCCCCGCCCTGAAGCGTTGATCGACGGCATTATGATGCTGCATCAAAAGGTGATGCGCGAGAAGATCAGTGGCAAGAAG
GAATCTCCCATCCGCATTGATCAGCCGCTTGTGCAGGTGAAGTAG

Upstream 100 bases:

>100_bases
TGTTTCGTCAGCTCGGCCCTGCCGGTCTTGCCGCCATGGGAGTCTTTTTCCTGGTGCTGCTCGTCGGCTTTGTCTATGAA
TGGAAGAAAGGGGCGTTGCG

Downstream 100 bases:

>100_bases
TATGGATAATCAAACGGTGCTTGCTGCATTACGTGAACGGTTTGCGGCCGAGATTCTGGCTGTCAATGAGTTTCGCGGCG
ATCTGAGTCTGACCTTCACG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 1; NDH-1 subunit B 1 [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MGIEEKAGNLGIVTTTLETVVNWGRTNAMWPLLFGLACCAIEMMGAQASNYDLSRFGMELNRASPRQADLMIVAGRVSRK
MAPVVRRLYDQMPEPKWVIAMGDCAACGGIFNNYAIVQGVDEVVPVDVYVAGCPPRPEALIDGIMMLHQKVMREKISGKK
ESPIRIDQPLVQVK

Sequences:

>Translated_174_residues
MGIEEKAGNLGIVTTTLETVVNWGRTNAMWPLLFGLACCAIEMMGAQASNYDLSRFGMELNRASPRQADLMIVAGRVSRK
MAPVVRRLYDQMPEPKWVIAMGDCAACGGIFNNYAIVQGVDEVVPVDVYVAGCPPRPEALIDGIMMLHQKVMREKISGKK
ESPIRIDQPLVQVK
>Mature_173_residues
GIEEKAGNLGIVTTTLETVVNWGRTNAMWPLLFGLACCAIEMMGAQASNYDLSRFGMELNRASPRQADLMIVAGRVSRKM
APVVRRLYDQMPEPKWVIAMGDCAACGGIFNNYAIVQGVDEVVPVDVYVAGCPPRPEALIDGIMMLHQKVMREKISGKKE
SPIRIDQPLVQVK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=144, Percent_Identity=53.4722222222222, Blast_Score=177, Evalue=5e-45,
Organism=Escherichia coli, GI1788624, Length=151, Percent_Identity=53.6423841059603, Blast_Score=186, Evalue=5e-49,
Organism=Escherichia coli, GI1789074, Length=137, Percent_Identity=37.2262773722628, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=37.7049180327869, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=140, Percent_Identity=54.2857142857143, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=55.7142857142857, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=55.7142857142857, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=56.4285714285714, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19139; Mature: 19008

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
9.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIEEKAGNLGIVTTTLETVVNWGRTNAMWPLLFGLACCAIEMMGAQASNYDLSRFGMEL
CCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NRASPRQADLMIVAGRVSRKMAPVVRRLYDQMPEPKWVIAMGDCAACGGIFNNYAIVQGV
CCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCHHHHHCC
DEVVPVDVYVAGCPPRPEALIDGIMMLHQKVMREKISGKKESPIRIDQPLVQVK
CHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEECC
>Mature Secondary Structure 
GIEEKAGNLGIVTTTLETVVNWGRTNAMWPLLFGLACCAIEMMGAQASNYDLSRFGMEL
CCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NRASPRQADLMIVAGRVSRKMAPVVRRLYDQMPEPKWVIAMGDCAACGGIFNNYAIVQGV
CCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCHHHHHCC
DEVVPVDVYVAGCPPRPEALIDGIMMLHQKVMREKISGKKESPIRIDQPLVQVK
CHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA