Click here to switch to the map view.

The map label for this gene is ygcM [C]

Identifier: 222526096

GI number: 222526096

Start: 3524001

End: 3524825

Strand: Direct

Name: ygcM [C]

Synonym: Chy400_2853

Alternate gene names: 222526096

Gene position: 3524001-3524825 (Clockwise)

Preceding gene: 222526092

Following gene: 222526097

Centisome position: 66.88

GC content: 55.03

Gene sequence:

>825_bases
ATGGTCGTTGCAACCCGTCGCTTTGAATTTTCGGCAGCTCATCGCTACTGGCGCGATGATTGGAGTGCGGAAGAAAATGA
GCGCGTCTTCGGGCCGTACACCAGCCCATACGGTCATGGGCATAACTATACACTCGATGTCAGCATTACCGGTGAGCTTG
ACGAGCGCACCGGGATGGTGATGAATATGACCGAACTGAAGGCTATCGTGAATGAGGTGCTCGAAGAGTTTGACCACAAG
CACCTCAATTACGATACACCTTACTTTCGCGATCAAGTGCCCACGACCGAGAATCTGGTGCGCGTACTCTGGCGTTTAAT
TGCAGCCCGCCTGCCTGCCCATGCGCGCCTGAAGCATCTCCGCCTCTATGAACAGCCCGATCTGTGGGCCGAATACGATG
GTGTTGGTGAGACCCAATTTGCTCGCCTGTACACCTTCGCCGCTGCCCATCGTCTGCATGCACCAGCCTTGAGTGATGAG
GAGAATCTGGCTATTTATGGCAAATGCAATAATCCTAATGGTCACGGTCACAACTATACGCTAGAGGTTACGGTACAGGG
GCCGATTGATCCGGCAACCGGTATGCTGGTCGATCTGGAGTGGCTTGACCGCACGGTACACTCGGTCATCGACCGATTGC
ATCTGCGCCACCTGGATCGTGAGATTCCGGCTTTTGCCGACCGCCCATCGACCGCTGAGAATATCATCGTTTACCTCTGG
AATGAACTTGCGCCGCTCCTTGAAGGTCGCCTGGCGCTCTTGCGCTTGTGGGAGACCCGCAAGAACATCTTTAGCTATGC
CGGTCCGTCGGTCATTCCCGCCTGA

Upstream 100 bases:

>100_bases
GAGACCTTGACTTTTTTCAGAGCCGTGGTACCGTTCATAAGGGAAGAGTTTTTATATTTTGATGATTGACACAGCGATGG
TTAACAGAAAGGATTATTCT

Downstream 100 bases:

>100_bases
TGGCACGTAATCCTGTGTTTCGTCGCCGAACGCGCACGTCGCGTTGTTCGTGCCTGTAATGGAGAACACATCTATGTCTA
GTAATGGTAACGGCTATCAC

Product: hypothetical protein

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MVVATRRFEFSAAHRYWRDDWSAEENERVFGPYTSPYGHGHNYTLDVSITGELDERTGMVMNMTELKAIVNEVLEEFDHK
HLNYDTPYFRDQVPTTENLVRVLWRLIAARLPAHARLKHLRLYEQPDLWAEYDGVGETQFARLYTFAAAHRLHAPALSDE
ENLAIYGKCNNPNGHGHNYTLEVTVQGPIDPATGMLVDLEWLDRTVHSVIDRLHLRHLDREIPAFADRPSTAENIIVYLW
NELAPLLEGRLALLRLWETRKNIFSYAGPSVIPA

Sequences:

>Translated_274_residues
MVVATRRFEFSAAHRYWRDDWSAEENERVFGPYTSPYGHGHNYTLDVSITGELDERTGMVMNMTELKAIVNEVLEEFDHK
HLNYDTPYFRDQVPTTENLVRVLWRLIAARLPAHARLKHLRLYEQPDLWAEYDGVGETQFARLYTFAAAHRLHAPALSDE
ENLAIYGKCNNPNGHGHNYTLEVTVQGPIDPATGMLVDLEWLDRTVHSVIDRLHLRHLDREIPAFADRPSTAENIIVYLW
NELAPLLEGRLALLRLWETRKNIFSYAGPSVIPA
>Mature_274_residues
MVVATRRFEFSAAHRYWRDDWSAEENERVFGPYTSPYGHGHNYTLDVSITGELDERTGMVMNMTELKAIVNEVLEEFDHK
HLNYDTPYFRDQVPTTENLVRVLWRLIAARLPAHARLKHLRLYEQPDLWAEYDGVGETQFARLYTFAAAHRLHAPALSDE
ENLAIYGKCNNPNGHGHNYTLEVTVQGPIDPATGMLVDLEWLDRTVHSVIDRLHLRHLDREIPAFADRPSTAENIIVYLW
NELAPLLEGRLALLRLWETRKNIFSYAGPSVIPA

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=134, Percent_Identity=47.7611940298507, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI115532226, Length=129, Percent_Identity=42.6356589147287, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI115532224, Length=129, Percent_Identity=42.6356589147287, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19550074, Length=133, Percent_Identity=48.8721804511278, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24585378, Length=133, Percent_Identity=48.8721804511278, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 31630; Mature: 31630

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00987 PTPS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVATRRFEFSAAHRYWRDDWSAEENERVFGPYTSPYGHGHNYTLDVSITGELDERTGMV
CEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCCEE
MNMTELKAIVNEVLEEFDHKHLNYDTPYFRDQVPTTENLVRVLWRLIAARLPAHARLKHL
ECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
RLYEQPDLWAEYDGVGETQFARLYTFAAAHRLHAPALSDEENLAIYGKCNNPNGHGHNYT
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEE
LEVTVQGPIDPATGMLVDLEWLDRTVHSVIDRLHLRHLDREIPAFADRPSTAENIIVYLW
EEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEHH
NELAPLLEGRLALLRLWETRKNIFSYAGPSVIPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVVATRRFEFSAAHRYWRDDWSAEENERVFGPYTSPYGHGHNYTLDVSITGELDERTGMV
CEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCCEE
MNMTELKAIVNEVLEEFDHKHLNYDTPYFRDQVPTTENLVRVLWRLIAARLPAHARLKHL
ECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
RLYEQPDLWAEYDGVGETQFARLYTFAAAHRLHAPALSDEENLAIYGKCNNPNGHGHNYT
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEE
LEVTVQGPIDPATGMLVDLEWLDRTVHSVIDRLHLRHLDREIPAFADRPSTAENIIVYLW
EEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEHH
NELAPLLEGRLALLRLWETRKNIFSYAGPSVIPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA