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The map label for this gene is folE [H]

Identifier: 222526097

GI number: 222526097

Start: 3524899

End: 3525582

Strand: Direct

Name: folE [H]

Synonym: Chy400_2854

Alternate gene names: 222526097

Gene position: 3524899-3525582 (Clockwise)

Preceding gene: 222526096

Following gene: 222526098

Centisome position: 66.9

GC content: 53.65

Gene sequence:

>684_bases
ATGTCTAGTAATGGTAACGGCTATCACCACCCAACTCCTGAGCTTGATGAGGAGCACTACGAGAGTTTGATCATCCCCGG
TCGAGGGAAACTTGAAGGTATGTCATTTAACGCCGATCCTCGGATTGAGGAGGCGGTACGGACAATTCTGGCTGCTATTG
GTGAGGTTCCGGATCGCGAAGGGTTGCAGAAGACACCGTCACGAGTGGCCAAGATGTATGCCGAACTGACGGCAGGTTAC
CACATTGATCCGCAGGCGTTGATCAACGGTGCTGTCTTCAGCGTGGCCTACGATGAGATGGTATTGGTCACCAACATCGA
CTACTACAGTCTCTGTGAACACCACATGCTGCCATTCTTTGGTCAGGTTCACGTGGCCTATATCCCCAATGGCAAAGTTG
TCGGTCTAAGTAAGATACCACGCATCGTTGAGATGTTTGCCCGGCGCTTGCAAGTGCAGGAACGGATGACGGTGCAGATC
GCAGACTTCATCAATGCCACCCTTGAACCTGCCGGTGTCGCAGTCGTGGCCGAAGGCATTCACATGTGTGGCGTCATGCG
CGGTGTCAAGAAGGCCAATGCCCGCATGGTAACCTCGGCAATGCGGGGCGTGTTCCGCGAAGACCCCAAGACGAGGGCCG
AGTTTATGGCGCATATTAACCGGGTGAATAATCGTCAGGAATGA

Upstream 100 bases:

>100_bases
GCCGGTCCGTCGGTCATTCCCGCCTGATGGCACGTAATCCTGTGTTTCGTCGCCGAACGCGCACGTCGCGTTGTTCGTGC
CTGTAATGGAGAACACATCT

Downstream 100 bases:

>100_bases
GTCTTTCGCACGCACCTGCACACGACAGCTATTTGCGCTTATCTGCCAGCGCCATGTCCGGGAACCCGGCAGTGTAGCCG
ATAGGCGATAACAACGTTGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSSNGNGYHHPTPELDEEHYESLIIPGRGKLEGMSFNADPRIEEAVRTILAAIGEVPDREGLQKTPSRVAKMYAELTAGY
HIDPQALINGAVFSVAYDEMVLVTNIDYYSLCEHHMLPFFGQVHVAYIPNGKVVGLSKIPRIVEMFARRLQVQERMTVQI
ADFINATLEPAGVAVVAEGIHMCGVMRGVKKANARMVTSAMRGVFREDPKTRAEFMAHINRVNNRQE

Sequences:

>Translated_227_residues
MSSNGNGYHHPTPELDEEHYESLIIPGRGKLEGMSFNADPRIEEAVRTILAAIGEVPDREGLQKTPSRVAKMYAELTAGY
HIDPQALINGAVFSVAYDEMVLVTNIDYYSLCEHHMLPFFGQVHVAYIPNGKVVGLSKIPRIVEMFARRLQVQERMTVQI
ADFINATLEPAGVAVVAEGIHMCGVMRGVKKANARMVTSAMRGVFREDPKTRAEFMAHINRVNNRQE
>Mature_226_residues
SSNGNGYHHPTPELDEEHYESLIIPGRGKLEGMSFNADPRIEEAVRTILAAIGEVPDREGLQKTPSRVAKMYAELTAGYH
IDPQALINGAVFSVAYDEMVLVTNIDYYSLCEHHMLPFFGQVHVAYIPNGKVVGLSKIPRIVEMFARRLQVQERMTVQIA
DFINATLEPAGVAVVAEGIHMCGVMRGVKKANARMVTSAMRGVFREDPKTRAEFMAHINRVNNRQE

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=180, Percent_Identity=54.4444444444444, Blast_Score=208, Evalue=3e-54,
Organism=Homo sapiens, GI4503949, Length=180, Percent_Identity=54.4444444444444, Blast_Score=208, Evalue=3e-54,
Organism=Homo sapiens, GI66932970, Length=150, Percent_Identity=48, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI66932972, Length=140, Percent_Identity=50.7142857142857, Blast_Score=151, Evalue=5e-37,
Organism=Escherichia coli, GI1788476, Length=158, Percent_Identity=40.5063291139241, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=56.4971751412429, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=53.2967032967033, Blast_Score=189, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24656782, Length=225, Percent_Identity=48.4444444444444, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24656796, Length=181, Percent_Identity=54.1436464088398, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24656791, Length=181, Percent_Identity=54.1436464088398, Blast_Score=197, Evalue=7e-51,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 25309; Mature: 25177

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNGNGYHHPTPELDEEHYESLIIPGRGKLEGMSFNADPRIEEAVRTILAAIGEVPDRE
CCCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
GLQKTPSRVAKMYAELTAGYHIDPQALINGAVFSVAYDEMVLVTNIDYYSLCEHHMLPFF
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEHHCCEEEEECCCHHHHHHHHCCCHH
GQVHVAYIPNGKVVGLSKIPRIVEMFARRLQVQERMTVQIADFINATLEPAGVAVVAEGI
CCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HMCGVMRGVKKANARMVTSAMRGVFREDPKTRAEFMAHINRVNNRQE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSNGNGYHHPTPELDEEHYESLIIPGRGKLEGMSFNADPRIEEAVRTILAAIGEVPDRE
CCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
GLQKTPSRVAKMYAELTAGYHIDPQALINGAVFSVAYDEMVLVTNIDYYSLCEHHMLPFF
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEHHCCEEEEECCCHHHHHHHHCCCHH
GQVHVAYIPNGKVVGLSKIPRIVEMFARRLQVQERMTVQIADFINATLEPAGVAVVAEGI
CCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HMCGVMRGVKKANARMVTSAMRGVFREDPKTRAEFMAHINRVNNRQE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]