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The map label for this gene is glyA [H]

Identifier: 222525988

GI number: 222525988

Start: 3381892

End: 3383151

Strand: Direct

Name: glyA [H]

Synonym: Chy400_2744

Alternate gene names: 222525988

Gene position: 3381892-3383151 (Clockwise)

Preceding gene: 222525987

Following gene: 222525990

Centisome position: 64.19

GC content: 58.41

Gene sequence:

>1260_bases
ATGCTTGAACATCTTCGAGCAACTGATCCGATCATCGCCGATCTGATCGAGCGGGAAGCGCAACGCCAGCGCCAGGGTCT
GGAGTTAATCGCCAGCGAGAACTATACCAGCCTGGCGGTGATGGAGGCGCAGGGATCGGTCTTGACCAATAAGTACGCAG
AGGGTTTGCCCGGTCGGCGATATTACGGTGGTTGCGAGTTTGTTGACGCCATCGAGCAACTGGCTATTGAACGGGCCTGT
CAGTTGTTCGGCACGTCCCACGCTAATGTGCAACCGCACAGTGGGGCGCAGGCCAATATCGCCGTGTTTACGGCTCTGCT
CCAGCCCGGTGATACCATTCTCGGTATGCGCCTGGATCACGGTGGGCATCTGACGCACGGTAGCCCGGTCAATTTCTCCG
GTAAGTGGTACAACGTCCATTTCTACGGTGTGGACGCGCAAACCGGTCAGATCGACTATGACGATCTGGCAAGCAAAGCA
CGGGCAATCCGGCCAAAGCTCATCACGTCGGGAGCAAGTGCATACCCGCGTATCATCGATTTTGCCCGGATGCGTCAGAT
CGCCGATGAGGTTGGCGCCCTGTTGATGGCCGATATTGCCCATATCGCCGGTCTGGTGGCTGCCGGCGAGCATCCATCAC
CCGTCGGTCACGCTCATGTGATTACCACAACTACCCATAAGACGTTACGCGGGCCACGGGGCGGCTTGATCTTGATGGGT
GATGATTTTGCGAAACAACTCAACTCAAGCGTCTTCCCCGGTACGCAGGGTGGGCCATTGATGCACGTGATCGCCGGTAA
GGCAGTTGCTTTCGGTGAAGCATTGCGGCCTGAGTTCAGGCAGTATGCGGCGCAAATCCGCCGCAATGCCCGTGCGCTGG
CCGAGGGATTGATGGCGCAGGGGCTGACCCTGGTTAGCGGTGGGACTGATAATCATCTGATGCTGGTCGATCTGCGCAGT
ACGGGCCTAACCGGCGCACAGGCACAGCGCGCACTTGATAAAGCGGCGATTACGGTCAACAAAAATGCCATCCCCGATGA
TCCACAACCGCCAATGAAGACGAGTGGGATTCGGATCGGGACACCCGCGGTAACGACCCGCGGTATGCGCGAACCAGAGA
TGGCTCAGATTGCGGCCTGGATTGGCGAGGTGCTGATGTACCCCGATGATGAAGCCCGCCTGAATCGGATCGCAGGCGAA
GTCGCCGATTTGTGCCGGCATTTCCCGGTGCCTGCTGATATGGTGCAGGTACGGGGGTAA

Upstream 100 bases:

>100_bases
TGGCCTGCTCCTCAAGACGATGATGCTGAGGGCAGGGTGGTGCACACTTTACTTGTGAACAGGTGTTGTAACTGAGGTCA
TAGTAAACAGGGGAACGACT

Downstream 100 bases:

>100_bases
TCCGGTCGCCGGGAGAGAATGAGGGTTAACAACATCCATTCCTGACGCACCGGGAGCACACGCTTCCGCCGGGCAACACA
TGAGTCTGGATGTTATCGAT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLEHLRATDPIIADLIEREAQRQRQGLELIASENYTSLAVMEAQGSVLTNKYAEGLPGRRYYGGCEFVDAIEQLAIERAC
QLFGTSHANVQPHSGAQANIAVFTALLQPGDTILGMRLDHGGHLTHGSPVNFSGKWYNVHFYGVDAQTGQIDYDDLASKA
RAIRPKLITSGASAYPRIIDFARMRQIADEVGALLMADIAHIAGLVAAGEHPSPVGHAHVITTTTHKTLRGPRGGLILMG
DDFAKQLNSSVFPGTQGGPLMHVIAGKAVAFGEALRPEFRQYAAQIRRNARALAEGLMAQGLTLVSGGTDNHLMLVDLRS
TGLTGAQAQRALDKAAITVNKNAIPDDPQPPMKTSGIRIGTPAVTTRGMREPEMAQIAAWIGEVLMYPDDEARLNRIAGE
VADLCRHFPVPADMVQVRG

Sequences:

>Translated_419_residues
MLEHLRATDPIIADLIEREAQRQRQGLELIASENYTSLAVMEAQGSVLTNKYAEGLPGRRYYGGCEFVDAIEQLAIERAC
QLFGTSHANVQPHSGAQANIAVFTALLQPGDTILGMRLDHGGHLTHGSPVNFSGKWYNVHFYGVDAQTGQIDYDDLASKA
RAIRPKLITSGASAYPRIIDFARMRQIADEVGALLMADIAHIAGLVAAGEHPSPVGHAHVITTTTHKTLRGPRGGLILMG
DDFAKQLNSSVFPGTQGGPLMHVIAGKAVAFGEALRPEFRQYAAQIRRNARALAEGLMAQGLTLVSGGTDNHLMLVDLRS
TGLTGAQAQRALDKAAITVNKNAIPDDPQPPMKTSGIRIGTPAVTTRGMREPEMAQIAAWIGEVLMYPDDEARLNRIAGE
VADLCRHFPVPADMVQVRG
>Mature_419_residues
MLEHLRATDPIIADLIEREAQRQRQGLELIASENYTSLAVMEAQGSVLTNKYAEGLPGRRYYGGCEFVDAIEQLAIERAC
QLFGTSHANVQPHSGAQANIAVFTALLQPGDTILGMRLDHGGHLTHGSPVNFSGKWYNVHFYGVDAQTGQIDYDDLASKA
RAIRPKLITSGASAYPRIIDFARMRQIADEVGALLMADIAHIAGLVAAGEHPSPVGHAHVITTTTHKTLRGPRGGLILMG
DDFAKQLNSSVFPGTQGGPLMHVIAGKAVAFGEALRPEFRQYAAQIRRNARALAEGLMAQGLTLVSGGTDNHLMLVDLRS
TGLTGAQAQRALDKAAITVNKNAIPDDPQPPMKTSGIRIGTPAVTTRGMREPEMAQIAAWIGEVLMYPDDEARLNRIAGE
VADLCRHFPVPADMVQVRG

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=454, Percent_Identity=46.2555066079295, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI261862350, Length=454, Percent_Identity=46.2555066079295, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI261862348, Length=454, Percent_Identity=46.2555066079295, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI19923315, Length=454, Percent_Identity=46.2555066079295, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI261862346, Length=450, Percent_Identity=45.7777777777778, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=47.8802992518703, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI22547189, Length=386, Percent_Identity=46.6321243523316, Blast_Score=318, Evalue=9e-87,
Organism=Escherichia coli, GI1788902, Length=400, Percent_Identity=62.25, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI25144732, Length=406, Percent_Identity=47.7832512315271, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25144729, Length=406, Percent_Identity=47.7832512315271, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319739, Length=455, Percent_Identity=42.8571428571429, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=430, Percent_Identity=42.3255813953488, Blast_Score=343, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=45.8797327394209, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=45.8797327394209, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45070; Mature: 45070

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEHLRATDPIIADLIEREAQRQRQGLELIASENYTSLAVMEAQGSVLTNKYAEGLPGRR
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCC
YYGGCEFVDAIEQLAIERACQLFGTSHANVQPHSGAQANIAVFTALLQPGDTILGMRLDH
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECC
GGHLTHGSPVNFSGKWYNVHFYGVDAQTGQIDYDDLASKARAIRPKLITSGASAYPRIID
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHH
FARMRQIADEVGALLMADIAHIAGLVAAGEHPSPVGHAHVITTTTHKTLRGPRGGLILMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHCCCCCCEEEEC
DDFAKQLNSSVFPGTQGGPLMHVIAGKAVAFGEALRPEFRQYAAQIRRNARALAEGLMAQ
CHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLMLVDLRSTGLTGAQAQRALDKAAITVNKNAIPDDPQPPMKTSGIRIG
CEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEEC
TPAVTTRGMREPEMAQIAAWIGEVLMYPDDEARLNRIAGEVADLCRHFPVPADMVQVRG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEECC
>Mature Secondary Structure
MLEHLRATDPIIADLIEREAQRQRQGLELIASENYTSLAVMEAQGSVLTNKYAEGLPGRR
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCC
YYGGCEFVDAIEQLAIERACQLFGTSHANVQPHSGAQANIAVFTALLQPGDTILGMRLDH
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECC
GGHLTHGSPVNFSGKWYNVHFYGVDAQTGQIDYDDLASKARAIRPKLITSGASAYPRIID
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHH
FARMRQIADEVGALLMADIAHIAGLVAAGEHPSPVGHAHVITTTTHKTLRGPRGGLILMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHCCCCCCEEEEC
DDFAKQLNSSVFPGTQGGPLMHVIAGKAVAFGEALRPEFRQYAAQIRRNARALAEGLMAQ
CHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLMLVDLRSTGLTGAQAQRALDKAAITVNKNAIPDDPQPPMKTSGIRIG
CEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEEC
TPAVTTRGMREPEMAQIAAWIGEVLMYPDDEARLNRIAGEVADLCRHFPVPADMVQVRG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA