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The map label for this gene is ams [H]

Identifier: 222525984

GI number: 222525984

Start: 3378133

End: 3380091

Strand: Direct

Name: ams [H]

Synonym: Chy400_2740

Alternate gene names: 222525984

Gene position: 3378133-3380091 (Clockwise)

Preceding gene: 222525981

Following gene: 222525987

Centisome position: 64.11

GC content: 55.59

Gene sequence:

>1959_bases
ATGCACGATTGGGAACGAATTCAACGGGAAGCAGCACGGTCATTGACCAGACTATTACCGCGGGTTGCGCAGGCATTTGC
CGAAGCCGGTGGCGCTGCGGGGGAATGGAATGTATTTGAGCAACGCCTGCGGCGAGAGTGGCCGCGCCTCTTTGAATTGC
TATTCGGTCTCTACGGTACGCAATATGACTTCTTCTACCACCTTGAACAATTGTTGCTGGCAATGGCGCAGAGCTGGTTG
AAACGACCGGATTGGCTCAAACAGCGTGATGCGTTGCGTGAAGCTGATACCGAGTGGTTTCAATCGGAACGCATGATGGG
GGGTGTCCTCTACGTTGATCGTTTCTGCGGGACACTTGCCCGTTTGCGCGAGTTTATTCCATATTTTCGCGAGCTGGGAT
TAACCTACCTTCACCTCATGCCCCTGTTTGAAGCGCCGGAGGGCAACAACGATGGTGGCTATGCGGTAAGTAGTTACCGA
CGGGTTAATCCACATATCGGGACAACTGCCGAACTGGCCGATCTGGCTCGTGAGCTTGATACTGCTGGGATTAGTCTTGT
GCTCGATTTTGTGTTTAACCACACATCTGACGAGCATGAGTGGGCGCGACGGGCGCAGGCCGGTGACCCCGATTATCAAG
AGTTTTACTTCCTCTTCCCGGATCGAACCATGCCTGATGCCTATGAGCGGACATTGCGCGAAATTTTTCCAACCGTGCGA
CGCGGGAGCTTCACGTGGCGGCCCGATATGCAGCGCTGGGTTTGGACAACCTTTAACAGTTTTCAGTGGGATCTCAATTA
CGCCAATCCGGCAGTGTTTCGGGCAATGGCTGCCGAGATGCTCTTCCTGGCCAATCTCGGCGTGGCTGTGTTGCGCCTCG
ATGCTGTCCCCTTCATCTGGAAGCGAATGGGAACAAATTGCGAGAATCAACCAGAAGCCCACCGGATTATTCAAGCCTTC
AACGCGATTGCGCGGATCACAGCGCCAGCTCTGCTCTTCAAATCGGAGGCGATTGTCCATCCCGATGACGTGCTAAGTTA
CGTCAGTCCACAGGAGTGTCAGCTCTCGTACAATCCACTGCTGATGGCATTGCTCTGGGAGACGCTGGCGACGCGGGAAG
TCAAGCTACTGGCACATTCCCTCAGCCATCGCTTCCGTCTACCGCCCGGATGTGCCTGGATCAATTACCTGCGCTCACAC
GATGATATTGGCTGGACATTTGATGATAACGATGCCCGTGCGGTGGGGATCGATCCCTGGGGGCATCGCCAGTTTCTGAA
TGCCTTCTACACCGGACGCTTTCCCGGTTCGTTTGCCCGTGGGTTGCCATTTCAAGAAAACCCCGATACCGGTGATGCGC
GCGTGTCCGGTACGCTGGCATCGCTGGCAGGTCTCGAACAGGCACTGGCTGGCGGCGACCCCCAGGCCATTGACATAGCA
ATTCGCCGGATTATCCTGCTTCACAGCATTGTGCTGAGTATCGGTGGTATTCCGTTGATCTACCTTGGCGATGAGGTTGG
CACGCTCAATGATTACGGGTACGTAAGCGACCCGGCGAAGGCTGACGATAGTCGCTGGGTGCATCGTCCGTTGCGCAATC
AGGTCGCGATGGATCAACGCCACGACCCGACAACGATACCTGGTCGGATCTTCAGTACGTTAACCCGACTGATCCATCTA
CGAACCTCCCTGCCGGCACTGCGCGATGGCATGATGGAGGTTGTGCGCAGCGATAACCGCCACGTGCTGGCCTATGTGCG
CCAAACCGGATCGCAGCGAGTACTGGTACTGGCCAATTTCTCGGAATATCCTCAGTCAATAGCCGGGAACCTGCTGCGTA
TGTACGGCCCTGGTCTTGAACTCTTCGACCTGATCCAGGAACAGCCGGTATCGGCCACAACAGCACTGACTCTACAGCCG
TATCAGTTTGTCTGGCTAACGCCGTCGTGGCAGAGATAA

Upstream 100 bases:

>100_bases
TGTGTTTTTCGACCATCTGTTGCCAGATTCATGCTTTTCAGCGATACTTGTGCTCTGGAAACTATTGCCCGACCGGGCGG
TGTATTGGCGAGGTTCAGAC

Downstream 100 bases:

>100_bases
ACAAATGGCAGGTGCGATCCAGAGGGTCGCACCTGCCACTACCTTCGAGCAAACGTCCTAACAAGCGTCCTTGAACAGCT
CTATCAACTCGTTAATCGTC

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MHDWERIQREAARSLTRLLPRVAQAFAEAGGAAGEWNVFEQRLRREWPRLFELLFGLYGTQYDFFYHLEQLLLAMAQSWL
KRPDWLKQRDALREADTEWFQSERMMGGVLYVDRFCGTLARLREFIPYFRELGLTYLHLMPLFEAPEGNNDGGYAVSSYR
RVNPHIGTTAELADLARELDTAGISLVLDFVFNHTSDEHEWARRAQAGDPDYQEFYFLFPDRTMPDAYERTLREIFPTVR
RGSFTWRPDMQRWVWTTFNSFQWDLNYANPAVFRAMAAEMLFLANLGVAVLRLDAVPFIWKRMGTNCENQPEAHRIIQAF
NAIARITAPALLFKSEAIVHPDDVLSYVSPQECQLSYNPLLMALLWETLATREVKLLAHSLSHRFRLPPGCAWINYLRSH
DDIGWTFDDNDARAVGIDPWGHRQFLNAFYTGRFPGSFARGLPFQENPDTGDARVSGTLASLAGLEQALAGGDPQAIDIA
IRRIILLHSIVLSIGGIPLIYLGDEVGTLNDYGYVSDPAKADDSRWVHRPLRNQVAMDQRHDPTTIPGRIFSTLTRLIHL
RTSLPALRDGMMEVVRSDNRHVLAYVRQTGSQRVLVLANFSEYPQSIAGNLLRMYGPGLELFDLIQEQPVSATTALTLQP
YQFVWLTPSWQR

Sequences:

>Translated_652_residues
MHDWERIQREAARSLTRLLPRVAQAFAEAGGAAGEWNVFEQRLRREWPRLFELLFGLYGTQYDFFYHLEQLLLAMAQSWL
KRPDWLKQRDALREADTEWFQSERMMGGVLYVDRFCGTLARLREFIPYFRELGLTYLHLMPLFEAPEGNNDGGYAVSSYR
RVNPHIGTTAELADLARELDTAGISLVLDFVFNHTSDEHEWARRAQAGDPDYQEFYFLFPDRTMPDAYERTLREIFPTVR
RGSFTWRPDMQRWVWTTFNSFQWDLNYANPAVFRAMAAEMLFLANLGVAVLRLDAVPFIWKRMGTNCENQPEAHRIIQAF
NAIARITAPALLFKSEAIVHPDDVLSYVSPQECQLSYNPLLMALLWETLATREVKLLAHSLSHRFRLPPGCAWINYLRSH
DDIGWTFDDNDARAVGIDPWGHRQFLNAFYTGRFPGSFARGLPFQENPDTGDARVSGTLASLAGLEQALAGGDPQAIDIA
IRRIILLHSIVLSIGGIPLIYLGDEVGTLNDYGYVSDPAKADDSRWVHRPLRNQVAMDQRHDPTTIPGRIFSTLTRLIHL
RTSLPALRDGMMEVVRSDNRHVLAYVRQTGSQRVLVLANFSEYPQSIAGNLLRMYGPGLELFDLIQEQPVSATTALTLQP
YQFVWLTPSWQR
>Mature_652_residues
MHDWERIQREAARSLTRLLPRVAQAFAEAGGAAGEWNVFEQRLRREWPRLFELLFGLYGTQYDFFYHLEQLLLAMAQSWL
KRPDWLKQRDALREADTEWFQSERMMGGVLYVDRFCGTLARLREFIPYFRELGLTYLHLMPLFEAPEGNNDGGYAVSSYR
RVNPHIGTTAELADLARELDTAGISLVLDFVFNHTSDEHEWARRAQAGDPDYQEFYFLFPDRTMPDAYERTLREIFPTVR
RGSFTWRPDMQRWVWTTFNSFQWDLNYANPAVFRAMAAEMLFLANLGVAVLRLDAVPFIWKRMGTNCENQPEAHRIIQAF
NAIARITAPALLFKSEAIVHPDDVLSYVSPQECQLSYNPLLMALLWETLATREVKLLAHSLSHRFRLPPGCAWINYLRSH
DDIGWTFDDNDARAVGIDPWGHRQFLNAFYTGRFPGSFARGLPFQENPDTGDARVSGTLASLAGLEQALAGGDPQAIDIA
IRRIILLHSIVLSIGGIPLIYLGDEVGTLNDYGYVSDPAKADDSRWVHRPLRNQVAMDQRHDPTTIPGRIFSTLTRLIHL
RTSLPALRDGMMEVVRSDNRHVLAYVRQTGSQRVLVLANFSEYPQSIAGNLLRMYGPGLELFDLIQEQPVSATTALTLQP
YQFVWLTPSWQR

Specific function: Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose s

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=188, Percent_Identity=26.063829787234, Blast_Score=67, Evalue=8e-11,
Organism=Escherichia coli, GI87081873, Length=557, Percent_Identity=25.8527827648115, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1790687, Length=186, Percent_Identity=31.1827956989247, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1786604, Length=94, Percent_Identity=38.2978723404255, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=195, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321731, Length=195, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=167, Percent_Identity=28.7425149700599, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=185, Percent_Identity=25.4054054054054, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=185, Percent_Identity=25.4054054054054, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=185, Percent_Identity=25.4054054054054, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321726, Length=195, Percent_Identity=25.1282051282051, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586593, Length=198, Percent_Identity=35.3535353535354, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583745, Length=187, Percent_Identity=33.6898395721925, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24583747, Length=182, Percent_Identity=35.7142857142857, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24583749, Length=182, Percent_Identity=35.7142857142857, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24586589, Length=204, Percent_Identity=33.8235294117647, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45549022, Length=186, Percent_Identity=31.1827956989247, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24586591, Length=200, Percent_Identity=28.5, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24586587, Length=188, Percent_Identity=30.8510638297872, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI221330053, Length=186, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281360393, Length=163, Percent_Identity=34.9693251533742, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24586597, Length=253, Percent_Identity=26.8774703557312, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24586599, Length=183, Percent_Identity=29.5081967213115, Blast_Score=84, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.4 [H]

Molecular weight: Translated: 74766; Mature: 74766

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDWERIQREAARSLTRLLPRVAQAFAEAGGAAGEWNVFEQRLRREWPRLFELLFGLYGT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
QYDFFYHLEQLLLAMAQSWLKRPDWLKQRDALREADTEWFQSERMMGGVLYVDRFCGTLA
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLREFIPYFRELGLTYLHLMPLFEAPEGNNDGGYAVSSYRRVNPHIGTTAELADLARELD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHCCCCCCCCHHHHHHHHHHHH
TAGISLVLDFVFNHTSDEHEWARRAQAGDPDYQEFYFLFPDRTMPDAYERTLREIFPTVR
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
RGSFTWRPDMQRWVWTTFNSFQWDLNYANPAVFRAMAAEMLFLANLGVAVLRLDAVPFIW
CCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KRMGTNCENQPEAHRIIQAFNAIARITAPALLFKSEAIVHPDDVLSYVSPQECQLSYNPL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHCCCCCCHH
LMALLWETLATREVKLLAHSLSHRFRLPPGCAWINYLRSHDDIGWTFDDNDARAVGIDPW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEECCCCCEEECCCCC
GHRQFLNAFYTGRFPGSFARGLPFQENPDTGDARVSGTLASLAGLEQALAGGDPQAIDIA
HHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
IRRIILLHSIVLSIGGIPLIYLGDEVGTLNDYGYVSDPAKADDSRWVHRPLRNQVAMDQR
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
HDPTTIPGRIFSTLTRLIHLRTSLPALRDGMMEVVRSDNRHVLAYVRQTGSQRVLVLANF
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEHHHHHCCCCEEEEEECC
SEYPQSIAGNLLRMYGPGLELFDLIQEQPVSATTALTLQPYQFVWLTPSWQR
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCC
>Mature Secondary Structure
MHDWERIQREAARSLTRLLPRVAQAFAEAGGAAGEWNVFEQRLRREWPRLFELLFGLYGT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
QYDFFYHLEQLLLAMAQSWLKRPDWLKQRDALREADTEWFQSERMMGGVLYVDRFCGTLA
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLREFIPYFRELGLTYLHLMPLFEAPEGNNDGGYAVSSYRRVNPHIGTTAELADLARELD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHCCCCCCCCHHHHHHHHHHHH
TAGISLVLDFVFNHTSDEHEWARRAQAGDPDYQEFYFLFPDRTMPDAYERTLREIFPTVR
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
RGSFTWRPDMQRWVWTTFNSFQWDLNYANPAVFRAMAAEMLFLANLGVAVLRLDAVPFIW
CCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KRMGTNCENQPEAHRIIQAFNAIARITAPALLFKSEAIVHPDDVLSYVSPQECQLSYNPL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHCCCCCCHH
LMALLWETLATREVKLLAHSLSHRFRLPPGCAWINYLRSHDDIGWTFDDNDARAVGIDPW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEECCCCCEEECCCCC
GHRQFLNAFYTGRFPGSFARGLPFQENPDTGDARVSGTLASLAGLEQALAGGDPQAIDIA
HHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
IRRIILLHSIVLSIGGIPLIYLGDEVGTLNDYGYVSDPAKADDSRWVHRPLRNQVAMDQR
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
HDPTTIPGRIFSTLTRLIHLRTSLPALRDGMMEVVRSDNRHVLAYVRQTGSQRVLVLANF
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEHHHHHCCCCEEEEEECC
SEYPQSIAGNLLRMYGPGLELFDLIQEQPVSATTALTLQPYQFVWLTPSWQR
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12517860 [H]