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The map label for this gene is tssA [H]

Identifier: 222525385

GI number: 222525385

Start: 2705705

End: 2706601

Strand: Direct

Name: tssA [H]

Synonym: Chy400_2130

Alternate gene names: 222525385

Gene position: 2705705-2706601 (Clockwise)

Preceding gene: 222525384

Following gene: 222525403

Centisome position: 51.35

GC content: 58.64

Gene sequence:

>897_bases
ATGAGCCTGGAATTACCATCAGGGCCACTCGTCGAAACCGACTGGCTGGCTGCCCATCTTGGGCATCCCAAACTCCGCAT
CGTTGACATGCGCGGTACCGTTTTGCCACCCTCTGCCCCTAAACCGCACTACTTTGCCCGCCACGACGACTACGCCGCCG
GGCATATTCCCGGCGCTGTCTACATTGATTGGACACGTGATATTGTTGATCTTGACGATCCAACCCCTGTGCAGGTAGCA
CCACCCGCCAAAATCGCTGAAGAATTGGGAAAGCGCGGTATCGGTGACGATTGTGTAGTGGTTGCCTACGACGACTGGTA
TTCGATGTTTGCCGGGCGTCTGATGTGGGTACTACGGTATTACGGGTTTGAGCAGGTGCGCGTGCTGAATGGTGGCATGG
TCAAATGGGTTGCCGAGGGCCGACCGTTGACGACGGAAGTGCCAGACTATCCGCCGGCGATCTTTACCCCTCGCCCCCAG
CCACACCTGCGTAAGACCGCCGACCAGGTGCTGCAAGCCCTGGGTAGCGATCTGCTCTTGATTGATGCCCGTCATGAACG
GGAGTATCAAGGCTTCGAGTCGCGTGCCGCTCGCGGCGGCCACATCCCCGGTGCACGCAATGTCTTTTATCAGAGCCTGG
TCAGCGGGCCGCATCAAACCTATCTCTCACCCGATCAACTCCGCGAACGTTTTCTTGCCGCCGGAATTGATCCTGACGCC
ACGACTGATCGTGAGGTCGTCGCCTACTGCAACGGCGGTGTTTCGGCGACACCCACAGCCATCGCCTTTGAACTGGTGAG
TGGTCGCCGGGTCGCGATCTACGATGGTTCGTGGAATGAGTGGGGTAACGATCCAACCAAACCGCTTGAACGAGCGGAAG
CGTCGTCGGGCGAGTAG

Upstream 100 bases:

>100_bases
ACTGTTTTAACCACAGAGACACAAAGGACACAGAGGTGGCTATTCTCTGTGCCTCTGTGGTGCAATAACCCACCAGCGTG
CTGATACAAAGAGGGATCGT

Downstream 100 bases:

>100_bases
TACCACATCAGTCGCAGCACGCTATCCAACTATCACCCTGGTACCGCATTTGAACGATGAACTGCTTCCCTCGTATGACA
ATGTGAAACAGCATATGCCC

Product: Rhodanese domain-containing protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSLELPSGPLVETDWLAAHLGHPKLRIVDMRGTVLPPSAPKPHYFARHDDYAAGHIPGAVYIDWTRDIVDLDDPTPVQVA
PPAKIAEELGKRGIGDDCVVVAYDDWYSMFAGRLMWVLRYYGFEQVRVLNGGMVKWVAEGRPLTTEVPDYPPAIFTPRPQ
PHLRKTADQVLQALGSDLLLIDARHEREYQGFESRAARGGHIPGARNVFYQSLVSGPHQTYLSPDQLRERFLAAGIDPDA
TTDREVVAYCNGGVSATPTAIAFELVSGRRVAIYDGSWNEWGNDPTKPLERAEASSGE

Sequences:

>Translated_298_residues
MSLELPSGPLVETDWLAAHLGHPKLRIVDMRGTVLPPSAPKPHYFARHDDYAAGHIPGAVYIDWTRDIVDLDDPTPVQVA
PPAKIAEELGKRGIGDDCVVVAYDDWYSMFAGRLMWVLRYYGFEQVRVLNGGMVKWVAEGRPLTTEVPDYPPAIFTPRPQ
PHLRKTADQVLQALGSDLLLIDARHEREYQGFESRAARGGHIPGARNVFYQSLVSGPHQTYLSPDQLRERFLAAGIDPDA
TTDREVVAYCNGGVSATPTAIAFELVSGRRVAIYDGSWNEWGNDPTKPLERAEASSGE
>Mature_297_residues
SLELPSGPLVETDWLAAHLGHPKLRIVDMRGTVLPPSAPKPHYFARHDDYAAGHIPGAVYIDWTRDIVDLDDPTPVQVAP
PAKIAEELGKRGIGDDCVVVAYDDWYSMFAGRLMWVLRYYGFEQVRVLNGGMVKWVAEGRPLTTEVPDYPPAIFTPRPQP
HLRKTADQVLQALGSDLLLIDARHEREYQGFESRAARGGHIPGARNVFYQSLVSGPHQTYLSPDQLRERFLAAGIDPDAT
TDREVVAYCNGGVSATPTAIAFELVSGRRVAIYDGSWNEWGNDPTKPLERAEASSGE

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=285, Percent_Identity=33.6842105263158, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI194473668, Length=282, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI61835204, Length=282, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI194473681, Length=282, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI87082121, Length=285, Percent_Identity=29.8245614035088, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081967, Length=258, Percent_Identity=29.4573643410853, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561888, Length=287, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71997283, Length=316, Percent_Identity=24.6835443037975, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324825, Length=248, Percent_Identity=25.8064516129032, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32957; Mature: 32826

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLELPSGPLVETDWLAAHLGHPKLRIVDMRGTVLPPSAPKPHYFARHDDYAAGHIPGAV
CCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCEE
YIDWTRDIVDLDDPTPVQVAPPAKIAEELGKRGIGDDCVVVAYDDWYSMFAGRLMWVLRY
EEEECCCEEECCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
YGFEQVRVLNGGMVKWVAEGRPLTTEVPDYPPAIFTPRPQPHLRKTADQVLQALGSDLLL
CCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
IDARHEREYQGFESRAARGGHIPGARNVFYQSLVSGPHQTYLSPDQLRERFLAAGIDPDA
EECCCCHHHHCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
TTDREVVAYCNGGVSATPTAIAFELVSGRRVAIYDGSWNEWGNDPTKPLERAEASSGE
CCCCEEEEEECCCCCCCCHHEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCC
>Mature Secondary Structure 
SLELPSGPLVETDWLAAHLGHPKLRIVDMRGTVLPPSAPKPHYFARHDDYAAGHIPGAV
CCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCEE
YIDWTRDIVDLDDPTPVQVAPPAKIAEELGKRGIGDDCVVVAYDDWYSMFAGRLMWVLRY
EEEECCCEEECCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
YGFEQVRVLNGGMVKWVAEGRPLTTEVPDYPPAIFTPRPQPHLRKTADQVLQALGSDLLL
CCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
IDARHEREYQGFESRAARGGHIPGARNVFYQSLVSGPHQTYLSPDQLRERFLAAGIDPDA
EECCCCHHHHCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
TTDREVVAYCNGGVSATPTAIAFELVSGRRVAIYDGSWNEWGNDPTKPLERAEASSGE
CCCCEEEEEECCCCCCCCHHEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA