Click here to switch to the map view.

The map label for this gene is pdhC [H]

Identifier: 222525384

GI number: 222525384

Start: 2704144

End: 2705496

Strand: Direct

Name: pdhC [H]

Synonym: Chy400_2129

Alternate gene names: 222525384

Gene position: 2704144-2705496 (Clockwise)

Preceding gene: 222525383

Following gene: 222525385

Centisome position: 51.32

GC content: 62.68

Gene sequence:

>1353_bases
ATGGGTGAAGTAACCATGCCTCGTCTCTCCGACACAATGTCGGAAGGTACTGTGGGCCGCTGGTTGAAGAAGGTCGGCGA
TCAGATCGCGGTGGGCGATATTATCGCCGAAATCGAGACCGATAAGGCCACAATGGAACTGGAAGCGTTTGAAGCCGGGG
TTTTGCAGCAGATTTTGATCCCGGAAGGGCAGACGGTACCGATTGGTCAGCCGATTGCCATCATTGGTGATAGTGCGGCC
CCGGTTGCGGCTGCGCCTGCACCAACCCCTGCACCGGCAGCCGCACCAGCTCCTGAGCCGGCAGCCGCACCGACCCCTGC
CCCAGCCCCCGCACTGGTGACGACCGCAGCGGCTCCCGGCGGTGGTGATGAAAATGGTCGGATCAAGGCCTCTCCGGTCG
CTCGTCGGTTGGCTGAAGAGTTGGGCATCGATCTCCGCCAGGTTGTTGGTACCGGCCCCGGCGGGCGGATTATCAAGGAA
AACGTGGAAGAGTTTGCTGCCCGGCGTGGTACCGCCGCTCCAGCCGCTGCTCCGGCACCGGCTCCCGCTCCAGCCGCTGT
CCCGGCACCGGCTCCCGCTCCAGCCGCTGCCTCGGCACCGGCTCCCACACCGGCACCGGCACCTGCCCCAACCCGTGCGC
CGGCACCGACTCCGGCTCCGGCACTGGCCGGCGCTGAGCCACTGAGCCGGATGCGCAAAGCGATTGCCCGGGCCATGACC
GACAGTAAACCGGGTGTGCCGCATATCTACCTCACTATCGAGGTGGATGCTGATGCGCTGATGGCGCTGCGCGAACAGAT
CACTGCCGGTGGTACCCGCGTCTCTGTCAACGATCTTGTGGTGAAGGCGGCGGCAAAGGCGCTGGCAAAGGTGCCGGCGG
TGAATGTCAGCTTCAGCCAGACCGCTGACGGCCAGCCGGGTATCGTGCGCCACAACCAGATCAATATCGGCGTTGCAGTT
GCACTCGACGATGGTCTGGTAGCGCCGGTTGTGCGCGATGCCGATAAGAAGAGCATTAGCGTGATTAGCGCCGAAATCCG
CGATATGGCGCTGCGTGCTCGCGAAGGGAAGATCAAGCAAAACGAACTCGAAGGGGCAACATTCCAGGTTACCAACCTCG
GTATGTTCGGCATCGTTGAGTTCGGGTCGATCATCTCGGTGCCCCAGGCTGCCTCGCTGGCCGTTGGTGCCGTGCGGAAG
GTACCGGTCGTGCGTGACGACCAGATCGTGATCGGACAGGTGATGAATCTCACCTTAAGCGCCGATCACCGGGTGATCGA
TGGTGCCGTGGGTGCGCAGTACCTGCAAGAGCTGCGCAAGCTGCTGGAAGCACCGATGAATATCATCGTGTAA

Upstream 100 bases:

>100_bases
CAAAGCCGAATGCTAACAGCCTGATTTACGCTATTCGACAGGTAATGCGCGGCACCAGGCGCATGCGATAACTGTTCTGT
AATGAATTACAGGAGGCTCC

Downstream 100 bases:

>100_bases
CGGTCCGTGACTGCCGGAACAAGCTCTGTGCCTCTGTGGTGTCGTATGTGTTAACCACAGAGACACAGAGCGGGCTATTC
TCTGTGCGCTCGGTGCCTCT

Product: dihydrolipoyllysine-residue succinyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MGEVTMPRLSDTMSEGTVGRWLKKVGDQIAVGDIIAEIETDKATMELEAFEAGVLQQILIPEGQTVPIGQPIAIIGDSAA
PVAAAPAPTPAPAAAPAPEPAAAPTPAPAPALVTTAAAPGGGDENGRIKASPVARRLAEELGIDLRQVVGTGPGGRIIKE
NVEEFAARRGTAAPAAAPAPAPAPAAVPAPAPAPAAASAPAPTPAPAPAPTRAPAPTPAPALAGAEPLSRMRKAIARAMT
DSKPGVPHIYLTIEVDADALMALREQITAGGTRVSVNDLVVKAAAKALAKVPAVNVSFSQTADGQPGIVRHNQINIGVAV
ALDDGLVAPVVRDADKKSISVISAEIRDMALRAREGKIKQNELEGATFQVTNLGMFGIVEFGSIISVPQAASLAVGAVRK
VPVVRDDQIVIGQVMNLTLSADHRVIDGAVGAQYLQELRKLLEAPMNIIV

Sequences:

>Translated_450_residues
MGEVTMPRLSDTMSEGTVGRWLKKVGDQIAVGDIIAEIETDKATMELEAFEAGVLQQILIPEGQTVPIGQPIAIIGDSAA
PVAAAPAPTPAPAAAPAPEPAAAPTPAPAPALVTTAAAPGGGDENGRIKASPVARRLAEELGIDLRQVVGTGPGGRIIKE
NVEEFAARRGTAAPAAAPAPAPAPAAVPAPAPAPAAASAPAPTPAPAPAPTRAPAPTPAPALAGAEPLSRMRKAIARAMT
DSKPGVPHIYLTIEVDADALMALREQITAGGTRVSVNDLVVKAAAKALAKVPAVNVSFSQTADGQPGIVRHNQINIGVAV
ALDDGLVAPVVRDADKKSISVISAEIRDMALRAREGKIKQNELEGATFQVTNLGMFGIVEFGSIISVPQAASLAVGAVRK
VPVVRDDQIVIGQVMNLTLSADHRVIDGAVGAQYLQELRKLLEAPMNIIV
>Mature_449_residues
GEVTMPRLSDTMSEGTVGRWLKKVGDQIAVGDIIAEIETDKATMELEAFEAGVLQQILIPEGQTVPIGQPIAIIGDSAAP
VAAAPAPTPAPAAAPAPEPAAAPTPAPAPALVTTAAAPGGGDENGRIKASPVARRLAEELGIDLRQVVGTGPGGRIIKEN
VEEFAARRGTAAPAAAPAPAPAPAAVPAPAPAPAAASAPAPTPAPAPAPTRAPAPTPAPALAGAEPLSRMRKAIARAMTD
SKPGVPHIYLTIEVDADALMALREQITAGGTRVSVNDLVVKAAAKALAKVPAVNVSFSQTADGQPGIVRHNQINIGVAVA
LDDGLVAPVVRDADKKSISVISAEIRDMALRAREGKIKQNELEGATFQVTNLGMFGIVEFGSIISVPQAASLAVGAVRKV
PVVRDDQIVIGQVMNLTLSADHRVIDGAVGAQYLQELRKLLEAPMNIIV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=472, Percent_Identity=38.5593220338983, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=33.7719298245614, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=33.7719298245614, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI110671329, Length=456, Percent_Identity=26.7543859649123, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=36.7469879518072, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI19923748, Length=259, Percent_Identity=27.7992277992278, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786946, Length=453, Percent_Identity=29.1390728476821, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1786305, Length=432, Percent_Identity=31.0185185185185, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=480, Percent_Identity=38.125, Blast_Score=267, Evalue=9e-72,
Organism=Caenorhabditis elegans, GI17537937, Length=451, Percent_Identity=26.1640798226164, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=322, Percent_Identity=30.7453416149068, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=464, Percent_Identity=28.448275862069, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=452, Percent_Identity=29.2035398230088, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=175, Percent_Identity=41.1428571428571, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321632, Length=172, Percent_Identity=31.9767441860465, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582497, Length=290, Percent_Identity=32.7586206896552, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI20129315, Length=290, Percent_Identity=32.7586206896552, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI18859875, Length=452, Percent_Identity=28.3185840707965, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=29.5154185022026, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45777; Mature: 45646

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVTMPRLSDTMSEGTVGRWLKKVGDQIAVGDIIAEIETDKATMELEAFEAGVLQQILI
CCCCCCCHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHCCCCHHEEHHHHHHHHHHHHHC
PEGQTVPIGQPIAIIGDSAAPVAAAPAPTPAPAAAPAPEPAAAPTPAPAPALVTTAAAPG
CCCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
GGDENGRIKASPVARRLAEELGIDLRQVVGTGPGGRIIKENVEEFAARRGTAAPAAAPAP
CCCCCCEEECCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
APAPAAVPAPAPAPAAASAPAPTPAPAPAPTRAPAPTPAPALAGAEPLSRMRKAIARAMT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DSKPGVPHIYLTIEVDADALMALREQITAGGTRVSVNDLVVKAAAKALAKVPAVNVSFSQ
CCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCEECCC
TADGQPGIVRHNQINIGVAVALDDGLVAPVVRDADKKSISVISAEIRDMALRAREGKIKQ
CCCCCCCEEEECEEEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCH
NELEGATFQVTNLGMFGIVEFGSIISVPQAASLAVGAVRKVPVVRDDQIVIGQVMNLTLS
HHCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHEEEEC
ADHRVIDGAVGAQYLQELRKLLEAPMNIIV
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GEVTMPRLSDTMSEGTVGRWLKKVGDQIAVGDIIAEIETDKATMELEAFEAGVLQQILI
CCCCCCHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHCCCCHHEEHHHHHHHHHHHHHC
PEGQTVPIGQPIAIIGDSAAPVAAAPAPTPAPAAAPAPEPAAAPTPAPAPALVTTAAAPG
CCCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
GGDENGRIKASPVARRLAEELGIDLRQVVGTGPGGRIIKENVEEFAARRGTAAPAAAPAP
CCCCCCEEECCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
APAPAAVPAPAPAPAAASAPAPTPAPAPAPTRAPAPTPAPALAGAEPLSRMRKAIARAMT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DSKPGVPHIYLTIEVDADALMALREQITAGGTRVSVNDLVVKAAAKALAKVPAVNVSFSQ
CCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCEECCC
TADGQPGIVRHNQINIGVAVALDDGLVAPVVRDADKKSISVISAEIRDMALRAREGKIKQ
CCCCCCCEEEECEEEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCH
NELEGATFQVTNLGMFGIVEFGSIISVPQAASLAVGAVRKVPVVRDDQIVIGQVMNLTLS
HHCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHEEEEC
ADHRVIDGAVGAQYLQELRKLLEAPMNIIV
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]