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The map label for this gene is 222524774

Identifier: 222524774

GI number: 222524774

Start: 1899878

End: 1901464

Strand: Direct

Name: 222524774

Synonym: Chy400_1501

Alternate gene names: NA

Gene position: 1899878-1901464 (Clockwise)

Preceding gene: 222524773

Following gene: 222524775

Centisome position: 36.06

GC content: 60.81

Gene sequence:

>1587_bases
ATGGACGCGCTTATTGAACGATTGCAGGCAGCCTTACACGCCGCAGGTATCCCGGCTACCGACACCGATTTCGCCGGCAT
TCTGGAAAAGGGCTTTCTCAGCCGGTTTGCCGATGTCGAACGCTTGCTCGATACCGTCGATCAAGAGCAATTGCCCGATA
TGCTCGACGCCACCAGTCTGCCGGTAAGCAGCGATCAGACAGACAGTGGTGACACTTCGCTGCCCGCCGACGATCTGAAC
ACCATCATGGGTGTCGCCGAAGAATTTCGGAAGCGCACCCTTTCATCGGTTGAAGTGGTTGAATGGGCGTTAGAGCAAAT
CGCGGAAAAGGATGCCCGCTTAAACGCATTTCAGATTGTGATGGCCGAAAGTGCCCTGGCCGACGCACGGGCTGCCGACG
CCGAAATCAGGGCCGGCACCATTCGCAGTCCACTGCACGGCGTACCGATAGCGGTGAAAGACCTGTTCGATGTCGCCGGT
TACCCGACCGCAGCCGGATCACGGATTCGCGCCGGGGTTATGGCGAGCAGCGATTCGACGGTGGTTGCGCGTTTACGGGC
AGCCGGGGCCATCATTATCGGCAAAACCCGGATGTCGGAATTCGCCTACTCACCCGGCTCGAATAATGCCCATTACGGTC
CGACTGCCAACCCCTACCATCCAGAGCGGGATAGCGGTGGCAGTAGCAGCGGTAGTGCGGTTGCTGTCAGTACGGTGATG
GCCTATGCCGCTATCGGCACTGACACTGGCGGCTCGATCCGGATACCGGCAGCGCACTGCGGGATCGTCGGTCTCAAACC
AACCCATGGCCGGGTAAGCCTGGCCGGCGGATTTCCCCTGTCGTGGTCACTCGATCATGCCGGCCCGCTGGCCCGCTCAG
TCGCCGATGCAGCCGTCATCCTCCAGGCTATCGCCGGGCCTGATCCACGCGATCACCGCACCTTGCGACCGGAGTCGCCT
TTTGCGGTAAGCGGTCTGCTAGATTATATTGATCAGGTACGAATTGGACTTCTTACCGCCGATGGCAGTGGTCAGGCAAT
AACCGATGCGGCTGGGCTGGCCGCAATGCAGCGCGTTGCCGACGCACTGGCTGAACGAGGCGCGACTGTCATACCGATTG
AAGTTCCCGAACTGGAAGCGTTACGGTTACTCAACCCGGCACTGCTGGCAATCGAAGCAGCCGCCTTGCATCTGCCATGG
CTACGCTCTCGCCTCGACGAGTATGGGGAATTTATGCGCCAGCGTATTCTGGCCGCCTTCATCTACTCGCCGGTAGATGC
CGTTCGCGTCCAACAGGCGCGGGCCAACCTGCGTCGGCGGGTGGCAGAACGTCTGGCCGGGATCGATGTCCTGATCACAC
CGGTCACACCGGGGCCGGCGCCGGCACTGGGAGTGCCGACACCAACGTCCTTTACCGGGCCATGGAACTTCCTGGGATGG
CCTGCTCTCAGCCTGCCAACCGGACGGGAAGAGAACGGGTTACCTCGCGCCGCCCAATTGATTGCCCGACCCTGGTGTGA
ATCGCTGCTCTTGCGCGTCGCGCAACATGCCGAGGATTTTATCGGGCACCTACCGCTGCCACTGTAA

Upstream 100 bases:

>100_bases
TGACTGCTACACGTTGAGTACACATTGGAGAAGGGATTGTTGCCGAGTCTTGCATGAATGATTACCACGCAAACCTTGCC
TCTGTTGAAAAGGAGCATCT

Downstream 100 bases:

>100_bases
CACACGGCGCTATGCTACAATTGGTGCCGGCGTCGCACAGCGTTGAAGCGACGAGAGCAGCATTGAGGAGCGCTGCGATG
TTTCGTACAATATTGGTTGC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MDALIERLQAALHAAGIPATDTDFAGILEKGFLSRFADVERLLDTVDQEQLPDMLDATSLPVSSDQTDSGDTSLPADDLN
TIMGVAEEFRKRTLSSVEVVEWALEQIAEKDARLNAFQIVMAESALADARAADAEIRAGTIRSPLHGVPIAVKDLFDVAG
YPTAAGSRIRAGVMASSDSTVVARLRAAGAIIIGKTRMSEFAYSPGSNNAHYGPTANPYHPERDSGGSSSGSAVAVSTVM
AYAAIGTDTGGSIRIPAAHCGIVGLKPTHGRVSLAGGFPLSWSLDHAGPLARSVADAAVILQAIAGPDPRDHRTLRPESP
FAVSGLLDYIDQVRIGLLTADGSGQAITDAAGLAAMQRVADALAERGATVIPIEVPELEALRLLNPALLAIEAAALHLPW
LRSRLDEYGEFMRQRILAAFIYSPVDAVRVQQARANLRRRVAERLAGIDVLITPVTPGPAPALGVPTPTSFTGPWNFLGW
PALSLPTGREENGLPRAAQLIARPWCESLLLRVAQHAEDFIGHLPLPL

Sequences:

>Translated_528_residues
MDALIERLQAALHAAGIPATDTDFAGILEKGFLSRFADVERLLDTVDQEQLPDMLDATSLPVSSDQTDSGDTSLPADDLN
TIMGVAEEFRKRTLSSVEVVEWALEQIAEKDARLNAFQIVMAESALADARAADAEIRAGTIRSPLHGVPIAVKDLFDVAG
YPTAAGSRIRAGVMASSDSTVVARLRAAGAIIIGKTRMSEFAYSPGSNNAHYGPTANPYHPERDSGGSSSGSAVAVSTVM
AYAAIGTDTGGSIRIPAAHCGIVGLKPTHGRVSLAGGFPLSWSLDHAGPLARSVADAAVILQAIAGPDPRDHRTLRPESP
FAVSGLLDYIDQVRIGLLTADGSGQAITDAAGLAAMQRVADALAERGATVIPIEVPELEALRLLNPALLAIEAAALHLPW
LRSRLDEYGEFMRQRILAAFIYSPVDAVRVQQARANLRRRVAERLAGIDVLITPVTPGPAPALGVPTPTSFTGPWNFLGW
PALSLPTGREENGLPRAAQLIARPWCESLLLRVAQHAEDFIGHLPLPL
>Mature_528_residues
MDALIERLQAALHAAGIPATDTDFAGILEKGFLSRFADVERLLDTVDQEQLPDMLDATSLPVSSDQTDSGDTSLPADDLN
TIMGVAEEFRKRTLSSVEVVEWALEQIAEKDARLNAFQIVMAESALADARAADAEIRAGTIRSPLHGVPIAVKDLFDVAG
YPTAAGSRIRAGVMASSDSTVVARLRAAGAIIIGKTRMSEFAYSPGSNNAHYGPTANPYHPERDSGGSSSGSAVAVSTVM
AYAAIGTDTGGSIRIPAAHCGIVGLKPTHGRVSLAGGFPLSWSLDHAGPLARSVADAAVILQAIAGPDPRDHRTLRPESP
FAVSGLLDYIDQVRIGLLTADGSGQAITDAAGLAAMQRVADALAERGATVIPIEVPELEALRLLNPALLAIEAAALHLPW
LRSRLDEYGEFMRQRILAAFIYSPVDAVRVQQARANLRRRVAERLAGIDVLITPVTPGPAPALGVPTPTSFTGPWNFLGW
PALSLPTGREENGLPRAAQLIARPWCESLLLRVAQHAEDFIGHLPLPL

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=491, Percent_Identity=31.1608961303462, Blast_Score=196, Evalue=6e-50,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=28.4552845528455, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI166795287, Length=460, Percent_Identity=26.0869565217391, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=442, Percent_Identity=29.6380090497738, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=481, Percent_Identity=25.987525987526, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=522, Percent_Identity=25.2873563218391, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=445, Percent_Identity=26.0674157303371, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=506, Percent_Identity=23.5177865612648, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=464, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=487, Percent_Identity=23.8193018480493, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=487, Percent_Identity=23.8193018480493, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=393, Percent_Identity=31.8066157760814, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323950, Length=437, Percent_Identity=25.4004576659039, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=31.304347826087, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648113, Length=489, Percent_Identity=33.5378323108384, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=30.2, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=30.2, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=30.2, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=30.2, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21356731, Length=478, Percent_Identity=28.0334728033473, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24644968, Length=485, Percent_Identity=26.3917525773196, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI161078093, Length=485, Percent_Identity=27.8350515463918, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24648435, Length=487, Percent_Identity=26.2833675564682, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648437, Length=487, Percent_Identity=26.2833675564682, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45550774, Length=487, Percent_Identity=26.2833675564682, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648441, Length=411, Percent_Identity=25.3041362530414, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648439, Length=411, Percent_Identity=25.3041362530414, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 55801; Mature: 55801

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALIERLQAALHAAGIPATDTDFAGILEKGFLSRFADVERLLDTVDQEQLPDMLDATSL
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCHHHHHHCCC
PVSSDQTDSGDTSLPADDLNTIMGVAEEFRKRTLSSVEVVEWALEQIAEKDARLNAFQIV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MAESALADARAADAEIRAGTIRSPLHGVPIAVKDLFDVAGYPTAAGSRIRAGVMASSDST
HHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHEECEEECCCCH
VVARLRAAGAIIIGKTRMSEFAYSPGSNNAHYGPTANPYHPERDSGGSSSGSAVAVSTVM
HHHHHHHCCEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AYAAIGTDTGGSIRIPAAHCGIVGLKPTHGRVSLAGGFPLSWSLDHAGPLARSVADAAVI
HHHHHCCCCCCEEECCHHHCCEEECCCCCCEEEECCCCCCEECCCCCCHHHHHHHHHHHH
LQAIAGPDPRDHRTLRPESPFAVSGLLDYIDQVRIGLLTADGSGQAITDAAGLAAMQRVA
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHH
DALAERGATVIPIEVPELEALRLLNPALLAIEAAALHLPWLRSRLDEYGEFMRQRILAAF
HHHHHCCCEEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYSPVDAVRVQQARANLRRRVAERLAGIDVLITPVTPGPAPALGVPTPTSFTGPWNFLGW
HHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PALSLPTGREENGLPRAAQLIARPWCESLLLRVAQHAEDFIGHLPLPL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDALIERLQAALHAAGIPATDTDFAGILEKGFLSRFADVERLLDTVDQEQLPDMLDATSL
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCHHHHHHCCC
PVSSDQTDSGDTSLPADDLNTIMGVAEEFRKRTLSSVEVVEWALEQIAEKDARLNAFQIV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MAESALADARAADAEIRAGTIRSPLHGVPIAVKDLFDVAGYPTAAGSRIRAGVMASSDST
HHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHEECEEECCCCH
VVARLRAAGAIIIGKTRMSEFAYSPGSNNAHYGPTANPYHPERDSGGSSSGSAVAVSTVM
HHHHHHHCCEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AYAAIGTDTGGSIRIPAAHCGIVGLKPTHGRVSLAGGFPLSWSLDHAGPLARSVADAAVI
HHHHHCCCCCCEEECCHHHCCEEECCCCCCEEEECCCCCCEECCCCCCHHHHHHHHHHHH
LQAIAGPDPRDHRTLRPESPFAVSGLLDYIDQVRIGLLTADGSGQAITDAAGLAAMQRVA
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHH
DALAERGATVIPIEVPELEALRLLNPALLAIEAAALHLPWLRSRLDEYGEFMRQRILAAF
HHHHHCCCEEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYSPVDAVRVQQARANLRRRVAERLAGIDVLITPVTPGPAPALGVPTPTSFTGPWNFLGW
HHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PALSLPTGREENGLPRAAQLIARPWCESLLLRVAQHAEDFIGHLPLPL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9809753 [H]