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The map label for this gene is pycA [H]

Identifier: 222524775

GI number: 222524775

Start: 1901542

End: 1903332

Strand: Direct

Name: pycA [H]

Synonym: Chy400_1502

Alternate gene names: 222524775

Gene position: 1901542-1903332 (Clockwise)

Preceding gene: 222524774

Following gene: 222524780

Centisome position: 36.09

GC content: 59.18

Gene sequence:

>1791_bases
ATGTTTCGTACAATATTGGTTGCCAATCGTGGAGAGATTGCCCTGCGCGTGATGCGCGCTTGCCGGGAATTGGGGTTGCG
GTGCGTCGCCGTCTATTCCGAAGCGGATCGCGACGCCCCGCACGTGGCGTATGCCGATGATGCCTTCCTGATCGGCCCAC
CTTCACCGGCTGAAAGTTATCTCAATATTGACGCAATTATCCGAGCGGCCAAAGCTACCGGTGCTGAGGCGATTCACCCC
GGTTATGGTTTTCTAGCTGAAAATGCCAGTTTTGTCCGTGCAGTCACCGCAGCCGGTCTGATCTTCATTGGCCCTCCCGC
CGAAGCCATGGAGCGCATGGGTGGCAAGACCGCTGCCCGCCGTGAAGCGACCGCTGCCGGCGTGCCGGTTGTTCCCGGTG
TGCTCGAACCGGTTACTGATGCGGCAGAGGTGCGGCGATTGGGGAAAGAATTCGGCTACCCGATTGCGATCAAAGCGGTA
GGCGGTGGCGGTGGGCGTGGTCTGCGCGTGGTGCGTTCACCGGAAGAGGTTGATGAAGCCTTCGCAGCAGCTCGGCGTGA
AGCTGAAGTCGCGTTCAAGAATGGTGAACTGTACGTCGAAAAATACCTTGACGATCCGCGACATATTGAAATCCAGGTGC
TGGCTGATCGTTATGGGAATGCGGTAGCTTTGGGGGAACGCGACTGTTCAGTACAGCGCCGCCATCAAAAACTGATCGAA
GAGTGCCCCTCGCCAGCCCTGACGCCCGAACTGCGGGCAGAGATGGGGGCTGCCGCGGTACGGCTGGCAAAAGCCGTCGG
GTATGTCAGCGCCGGCACGTTGGAGTTTCTCTTTCAGGATGGGCGCTACTACTTCCTGGAGATGAACACACGCATTCAGG
TCGAACACACCGTCACCGAGATGGTGTACGGCATCGATCTGGTTGCCGCGCAGATTCGGATTGCCCAGGGCGAGAAGCTC
TGGTTCAAACAAGAGGATGTAGTGCCCCGCGGCCACGCTATTGAGTGTCGGATCAATGCGGAAGACCCGCTCCACAACTT
TCGCCCGGCACTGGGCACCATCGGCGAATACCACGAGCCGGTTGGCTTCGGGGTACGGGTAGATAGCGGCGTGCGCGCCT
ACTATACCGTGCCGTCACATTACGACTCGCTGCTGGCCAAACTGATCACCTGGGGAAGTGACCGACAGGAAGCAATTGCC
CGTATGCGACGGGCACTGGCCGAGTACCGGATTGAGGGTGTCACGACCATTATTCCATTCCATCAGGCCGCGCTTGAACA
TCCTGTTTTTACGGCAGGTGCAGCGACCGTCAACTTTATTCCGCGCCATCCCGAACTCTTCTCCCGTGCGGCAGAGCTAA
CGCCACCAACGGCTGCTTCAGCAGGGGCTGAACCGGCACCGGAACCACGCCGATTTACCATCGAAGTCAATGGCCGGCGT
TTTGGGGTGGCCGTTTTTGGCGACGGTATGAATGCGACACCAGTTGCATCGCCATCACGTTCAGCACCTGCGCGCCGTGC
TGCCCCCAAGAAGACGACGCTGGCCGCGCCGGTTGATGCGGTGATCAGCCCAATCCAGGGTCGTGTCGTTGCGGTGCGGG
TGGCCCATGGGCAGCAGGTGGAAGCGGGCCAGGTGCTCTTCATTGTGGAAGCGATGAAGATGGAGAATGAGATCACCGCG
CCGCATAGTGGCACCATTGCTGAAGTACGCGTTGAGGTGGGAACGACGGTTGAAGCCGGGGCGATGCTGGCAACGTATCA
GAATACGGCGAATAACACGAATGGCAAATAG

Upstream 100 bases:

>100_bases
GGCACCTACCGCTGCCACTGTAACACACGGCGCTATGCTACAATTGGTGCCGGCGTCGCACAGCGTTGAAGCGACGAGAG
CAGCATTGAGGAGCGCTGCG

Downstream 100 bases:

>100_bases
GAACCTGTCGTGAAAACCGATCACACCGGTGGGCGAGGGATTTGCCTTCGCCCATTTTTATGTCAACTTCAACAGTCACA
TTATATCAAATCTGTCAGAA

Product: acetyl-CoA carboxylase, biotin carboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MFRTILVANRGEIALRVMRACRELGLRCVAVYSEADRDAPHVAYADDAFLIGPPSPAESYLNIDAIIRAAKATGAEAIHP
GYGFLAENASFVRAVTAAGLIFIGPPAEAMERMGGKTAARREATAAGVPVVPGVLEPVTDAAEVRRLGKEFGYPIAIKAV
GGGGGRGLRVVRSPEEVDEAFAAARREAEVAFKNGELYVEKYLDDPRHIEIQVLADRYGNAVALGERDCSVQRRHQKLIE
ECPSPALTPELRAEMGAAAVRLAKAVGYVSAGTLEFLFQDGRYYFLEMNTRIQVEHTVTEMVYGIDLVAAQIRIAQGEKL
WFKQEDVVPRGHAIECRINAEDPLHNFRPALGTIGEYHEPVGFGVRVDSGVRAYYTVPSHYDSLLAKLITWGSDRQEAIA
RMRRALAEYRIEGVTTIIPFHQAALEHPVFTAGAATVNFIPRHPELFSRAAELTPPTAASAGAEPAPEPRRFTIEVNGRR
FGVAVFGDGMNATPVASPSRSAPARRAAPKKTTLAAPVDAVISPIQGRVVAVRVAHGQQVEAGQVLFIVEAMKMENEITA
PHSGTIAEVRVEVGTTVEAGAMLATYQNTANNTNGK

Sequences:

>Translated_596_residues
MFRTILVANRGEIALRVMRACRELGLRCVAVYSEADRDAPHVAYADDAFLIGPPSPAESYLNIDAIIRAAKATGAEAIHP
GYGFLAENASFVRAVTAAGLIFIGPPAEAMERMGGKTAARREATAAGVPVVPGVLEPVTDAAEVRRLGKEFGYPIAIKAV
GGGGGRGLRVVRSPEEVDEAFAAARREAEVAFKNGELYVEKYLDDPRHIEIQVLADRYGNAVALGERDCSVQRRHQKLIE
ECPSPALTPELRAEMGAAAVRLAKAVGYVSAGTLEFLFQDGRYYFLEMNTRIQVEHTVTEMVYGIDLVAAQIRIAQGEKL
WFKQEDVVPRGHAIECRINAEDPLHNFRPALGTIGEYHEPVGFGVRVDSGVRAYYTVPSHYDSLLAKLITWGSDRQEAIA
RMRRALAEYRIEGVTTIIPFHQAALEHPVFTAGAATVNFIPRHPELFSRAAELTPPTAASAGAEPAPEPRRFTIEVNGRR
FGVAVFGDGMNATPVASPSRSAPARRAAPKKTTLAAPVDAVISPIQGRVVAVRVAHGQQVEAGQVLFIVEAMKMENEITA
PHSGTIAEVRVEVGTTVEAGAMLATYQNTANNTNGK
>Mature_596_residues
MFRTILVANRGEIALRVMRACRELGLRCVAVYSEADRDAPHVAYADDAFLIGPPSPAESYLNIDAIIRAAKATGAEAIHP
GYGFLAENASFVRAVTAAGLIFIGPPAEAMERMGGKTAARREATAAGVPVVPGVLEPVTDAAEVRRLGKEFGYPIAIKAV
GGGGGRGLRVVRSPEEVDEAFAAARREAEVAFKNGELYVEKYLDDPRHIEIQVLADRYGNAVALGERDCSVQRRHQKLIE
ECPSPALTPELRAEMGAAAVRLAKAVGYVSAGTLEFLFQDGRYYFLEMNTRIQVEHTVTEMVYGIDLVAAQIRIAQGEKL
WFKQEDVVPRGHAIECRINAEDPLHNFRPALGTIGEYHEPVGFGVRVDSGVRAYYTVPSHYDSLLAKLITWGSDRQEAIA
RMRRALAEYRIEGVTTIIPFHQAALEHPVFTAGAATVNFIPRHPELFSRAAELTPPTAASAGAEPAPEPRRFTIEVNGRR
FGVAVFGDGMNATPVASPSRSAPARRAAPKKTTLAAPVDAVISPIQGRVVAVRVAHGQQVEAGQVLFIVEAMKMENEITA
PHSGTIAEVRVEVGTTVEAGAMLATYQNTANNTNGK

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG4770

COG function: function code I; Acetyl/propionyl-CoA carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=451, Percent_Identity=49.2239467849224, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI295821183, Length=630, Percent_Identity=40.4761904761905, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI106049528, Length=452, Percent_Identity=49.3362831858407, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI106049295, Length=452, Percent_Identity=49.3362831858407, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI106049292, Length=452, Percent_Identity=49.3362831858407, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI189095269, Length=450, Percent_Identity=47.5555555555556, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI65506442, Length=450, Percent_Identity=47.5555555555556, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI38679960, Length=479, Percent_Identity=34.0292275574113, Blast_Score=262, Evalue=8e-70,
Organism=Homo sapiens, GI38679977, Length=479, Percent_Identity=34.0292275574113, Blast_Score=261, Evalue=9e-70,
Organism=Homo sapiens, GI38679967, Length=479, Percent_Identity=34.0292275574113, Blast_Score=261, Evalue=9e-70,
Organism=Homo sapiens, GI38679971, Length=509, Percent_Identity=33.0058939096267, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI38679974, Length=509, Percent_Identity=33.0058939096267, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI134142062, Length=476, Percent_Identity=33.6134453781513, Blast_Score=240, Evalue=3e-63,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=53.2731376975169, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71987519, Length=449, Percent_Identity=49.4432071269488, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17562816, Length=453, Percent_Identity=49.4481236203091, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17567343, Length=458, Percent_Identity=45.4148471615721, Blast_Score=394, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI133931226, Length=462, Percent_Identity=31.1688311688312, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI71997168, Length=406, Percent_Identity=33.743842364532, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI71997163, Length=406, Percent_Identity=34.2364532019704, Blast_Score=204, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319695, Length=452, Percent_Identity=48.4513274336283, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321376, Length=451, Percent_Identity=49.0022172949002, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319685, Length=463, Percent_Identity=40.3887688984881, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324343, Length=721, Percent_Identity=28.9875173370319, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6323863, Length=434, Percent_Identity=34.7926267281106, Blast_Score=243, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24652212, Length=453, Percent_Identity=50.551876379691, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652210, Length=453, Percent_Identity=50.551876379691, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652214, Length=453, Percent_Identity=50.551876379691, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI19921944, Length=453, Percent_Identity=50.551876379691, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652216, Length=453, Percent_Identity=50.551876379691, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI281363050, Length=453, Percent_Identity=50.551876379691, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652224, Length=453, Percent_Identity=50.551876379691, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652222, Length=453, Percent_Identity=50.551876379691, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652220, Length=453, Percent_Identity=50.551876379691, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652218, Length=453, Percent_Identity=50.551876379691, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651757, Length=662, Percent_Identity=37.6132930513595, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651759, Length=626, Percent_Identity=36.5814696485623, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24586458, Length=470, Percent_Identity=34.6808510638298, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI161076409, Length=470, Percent_Identity=34.6808510638298, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI161076407, Length=470, Percent_Identity=34.6808510638298, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24586460, Length=470, Percent_Identity=34.6808510638298, Blast_Score=246, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 64303; Mature: 64303

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50975 ATP_GRASP ; PS00867 CPSASE_2 ; PS50979 BC ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTILVANRGEIALRVMRACRELGLRCVAVYSEADRDAPHVAYADDAFLIGPPSPAESY
CCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCEEEECCCCCHHHH
LNIDAIIRAAKATGAEAIHPGYGFLAENASFVRAVTAAGLIFIGPPAEAMERMGGKTAAR
CCHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHCCEEEECCCHHHHHHCCCCHHHH
REATAAGVPVVPGVLEPVTDAAEVRRLGKEFGYPIAIKAVGGGGGRGLRVVRSPEEVDEA
HHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHHHH
FAAARREAEVAFKNGELYVEKYLDDPRHIEIQVLADRYGNAVALGERDCSVQRRHQKLIE
HHHHHHCCEEEEECCCEEEEECCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
ECPSPALTPELRAEMGAAAVRLAKAVGYVSAGTLEFLFQDGRYYFLEMNTRIQVEHTVTE
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCEEEEHHHHHH
MVYGIDLVAAQIRIAQGEKLWFKQEDVVPRGHAIECRINAEDPLHNFRPALGTIGEYHEP
HHHHHHHHEEEEEEECCCEEEEECCCCCCCCCEEEEEECCCCCCHHHCHHHHCCCCCCCC
VGFGVRVDSGVRAYYTVPSHYDSLLAKLITWGSDRQEAIARMRRALAEYRIEGVTTIIPF
CCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECH
HQAALEHPVFTAGAATVNFIPRHPELFSRAAELTPPTAASAGAEPAPEPRRFTIEVNGRR
HHHHHCCCCEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCEE
FGVAVFGDGMNATPVASPSRSAPARRAAPKKTTLAAPVDAVISPIQGRVVAVRVAHGQQV
EEEEEEECCCCCCCCCCCCCCCCCHHCCCCCCEECCCHHHHHCCCCCCEEEEEEECCCCC
EAGQVLFIVEAMKMENEITAPHSGTIAEVRVEVGTTVEAGAMLATYQNTANNTNGK
CCCCEEEEEEHHHHCCCCCCCCCCCEEEEEEEECCEECCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MFRTILVANRGEIALRVMRACRELGLRCVAVYSEADRDAPHVAYADDAFLIGPPSPAESY
CCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCEEEECCCCCHHHH
LNIDAIIRAAKATGAEAIHPGYGFLAENASFVRAVTAAGLIFIGPPAEAMERMGGKTAAR
CCHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHCCEEEECCCHHHHHHCCCCHHHH
REATAAGVPVVPGVLEPVTDAAEVRRLGKEFGYPIAIKAVGGGGGRGLRVVRSPEEVDEA
HHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHHHH
FAAARREAEVAFKNGELYVEKYLDDPRHIEIQVLADRYGNAVALGERDCSVQRRHQKLIE
HHHHHHCCEEEEECCCEEEEECCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
ECPSPALTPELRAEMGAAAVRLAKAVGYVSAGTLEFLFQDGRYYFLEMNTRIQVEHTVTE
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCEEEEHHHHHH
MVYGIDLVAAQIRIAQGEKLWFKQEDVVPRGHAIECRINAEDPLHNFRPALGTIGEYHEP
HHHHHHHHEEEEEEECCCEEEEECCCCCCCCCEEEEEECCCCCCHHHCHHHHCCCCCCCC
VGFGVRVDSGVRAYYTVPSHYDSLLAKLITWGSDRQEAIARMRRALAEYRIEGVTTIIPF
CCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECH
HQAALEHPVFTAGAATVNFIPRHPELFSRAAELTPPTAASAGAEPAPEPRRFTIEVNGRR
HHHHHCCCCEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCEE
FGVAVFGDGMNATPVASPSRSAPARRAAPKKTTLAAPVDAVISPIQGRVVAVRVAHGQQV
EEEEEEECCCCCCCCCCCCCCCCCHHCCCCCCEECCCHHHHHCCCCCCEEEEEEECCCCC
EAGQVLFIVEAMKMENEITAPHSGTIAEVRVEVGTTVEAGAMLATYQNTANNTNGK
CCCCEEEEEEHHHHCCCCCCCCCCCEEEEEEEECCEECCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]