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The map label for this gene is mhpE [C]

Identifier: 222524746

GI number: 222524746

Start: 1869049

End: 1870080

Strand: Direct

Name: mhpE [C]

Synonym: Chy400_1473

Alternate gene names: 222524746

Gene position: 1869049-1870080 (Clockwise)

Preceding gene: 222524745

Following gene: 222524747

Centisome position: 35.47

GC content: 57.17

Gene sequence:

>1032_bases
ATGAAACCACCACGGCTGACCGATACAACGCTCCGTGATGGCTCACATCCGATGCGTCATCAGTTTACCCGCCAGCAGGT
AGCTACGATTGTCCAGGCTCTCGACCGGGCCGGCGTGCCGGTGATTGAGGTGAGTCACGGCGATGGCTTGGCCGGTTCGT
CGCTGCAATATGGCTTCTCGCACACATCAGAATTTGATTTAATTGAAACGGCTCGTCAACATGCCGAAAGAGCGAAAATT
GCTGCCCTGATGCTACCGGGCATCGGCACCCGGCAAGAATTGAAAGAGGCGGTGGCCCGTGGGGTGCAAGTAGTACGGAT
TGCGACACAATGCACAGAGGCAGATATTTCTGAACAGCATTTTGGTCTGGCAAAAGAGCTGGGGTTAGAGACGGTTGGCT
TTCTGATGATGGCCCATATGCGTCCCCCCGAAGAGCTGGCCAGGCAGGCGAAATTAATGGAGTCTTACGGCGCCGACTGC
GTTTACATTGTCGACTCGGCGGGGGCGATGCTACCCCAGGATGCAGCAGCACGGGTGCAGGCGCTGAAAGACACCCTATC
AGTGCAGGTTGGATTCCACGCACACAACAACTTAGGGCTTGGTATCGCCAACACCCTGGCAGCGCTCGAAGCCGGTGCCG
ATCAGATCGATGGCTGTCTACGAGGTTTGGGGGCCGGTGCCGGCAACGCGGCAACCGAATTGCTGGCCGCAGTGCTTGAC
CGGCTGGGACTCAATCCTGGTCTTGATGTCTTCAGTCTCATGGATGCGGCTGAGTATGTGGTTGCCCCTATCATGCCCTT
CCAACCGTTCCCCGATCGGGATGCCATCACCATCGGCTATGCTGGTGTCTATTCAACATTTCTGCTACACGCCAAGCGCG
CCGGTGCTCAATACAACGTCGATCCGCGTGAAATTCTGGTCGAGTTGGGCCGTCGTCAGGCGGTAGCGGGCCAGGAAGAT
TGGATTATCGATGTTGCGCTCGAACTGAGTCGCCGGCATCAATCAAGTGCGCGAAAGGAGTCTCGCCCGTGA

Upstream 100 bases:

>100_bases
TCCCAACCTATGCCGGGAATCTCGACATCATGACTGCCGCCGCACGACGGGTGGGTGAACTATTCGCCGCACACCTCTTG
TCGAAACTGGAGGTGACCGT

Downstream 100 bases:

>100_bases
GCAATGCAGTATCAGTACGAGCAGAGGTTCCGGCCATCGATCCACGCCTCTTTCGCTCCACGATGGGCTGTTTTGCAACC
GGAGTGACGGTCGTCACGAC

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKPPRLTDTTLRDGSHPMRHQFTRQQVATIVQALDRAGVPVIEVSHGDGLAGSSLQYGFSHTSEFDLIETARQHAERAKI
AALMLPGIGTRQELKEAVARGVQVVRIATQCTEADISEQHFGLAKELGLETVGFLMMAHMRPPEELARQAKLMESYGADC
VYIVDSAGAMLPQDAAARVQALKDTLSVQVGFHAHNNLGLGIANTLAALEAGADQIDGCLRGLGAGAGNAATELLAAVLD
RLGLNPGLDVFSLMDAAEYVVAPIMPFQPFPDRDAITIGYAGVYSTFLLHAKRAGAQYNVDPREILVELGRRQAVAGQED
WIIDVALELSRRHQSSARKESRP

Sequences:

>Translated_343_residues
MKPPRLTDTTLRDGSHPMRHQFTRQQVATIVQALDRAGVPVIEVSHGDGLAGSSLQYGFSHTSEFDLIETARQHAERAKI
AALMLPGIGTRQELKEAVARGVQVVRIATQCTEADISEQHFGLAKELGLETVGFLMMAHMRPPEELARQAKLMESYGADC
VYIVDSAGAMLPQDAAARVQALKDTLSVQVGFHAHNNLGLGIANTLAALEAGADQIDGCLRGLGAGAGNAATELLAAVLD
RLGLNPGLDVFSLMDAAEYVVAPIMPFQPFPDRDAITIGYAGVYSTFLLHAKRAGAQYNVDPREILVELGRRQAVAGQED
WIIDVALELSRRHQSSARKESRP
>Mature_343_residues
MKPPRLTDTTLRDGSHPMRHQFTRQQVATIVQALDRAGVPVIEVSHGDGLAGSSLQYGFSHTSEFDLIETARQHAERAKI
AALMLPGIGTRQELKEAVARGVQVVRIATQCTEADISEQHFGLAKELGLETVGFLMMAHMRPPEELARQAKLMESYGADC
VYIVDSAGAMLPQDAAARVQALKDTLSVQVGFHAHNNLGLGIANTLAALEAGADQIDGCLRGLGAGAGNAATELLAAVLD
RLGLNPGLDVFSLMDAAEYVVAPIMPFQPFPDRDAITIGYAGVYSTFLLHAKRAGAQYNVDPREILVELGRRQAVAGQED
WIIDVALELSRRHQSSARKESRP

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=330, Percent_Identity=53.9393939393939, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1786261, Length=263, Percent_Identity=24.7148288973384, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_CHLAA (A9W9U3)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001634968.1
- ProteinModelPortal:   A9W9U3
- SMR:   A9W9U3
- GeneID:   5828456
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1351
- HOGENOM:   HBG300122
- OMA:   ELGSDAQ
- ProtClustDB:   PRK08195
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36951; Mature: 36951

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 166-166 BINDING 193-193 BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPRLTDTTLRDGSHPMRHQFTRQQVATIVQALDRAGVPVIEVSHGDGLAGSSLQYGFS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
HTSEFDLIETARQHAERAKIAALMLPGIGTRQELKEAVARGVQVVRIATQCTEADISEQH
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
FGLAKELGLETVGFLMMAHMRPPEELARQAKLMESYGADCVYIVDSAGAMLPQDAAARVQ
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
ALKDTLSVQVGFHAHNNLGLGIANTLAALEAGADQIDGCLRGLGAGAGNAATELLAAVLD
HHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RLGLNPGLDVFSLMDAAEYVVAPIMPFQPFPDRDAITIGYAGVYSTFLLHAKRAGAQYNV
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCC
DPREILVELGRRQAVAGQEDWIIDVALELSRRHQSSARKESRP
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPPRLTDTTLRDGSHPMRHQFTRQQVATIVQALDRAGVPVIEVSHGDGLAGSSLQYGFS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
HTSEFDLIETARQHAERAKIAALMLPGIGTRQELKEAVARGVQVVRIATQCTEADISEQH
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
FGLAKELGLETVGFLMMAHMRPPEELARQAKLMESYGADCVYIVDSAGAMLPQDAAARVQ
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
ALKDTLSVQVGFHAHNNLGLGIANTLAALEAGADQIDGCLRGLGAGAGNAATELLAAVLD
HHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RLGLNPGLDVFSLMDAAEYVVAPIMPFQPFPDRDAITIGYAGVYSTFLLHAKRAGAQYNV
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCC
DPREILVELGRRQAVAGQEDWIIDVALELSRRHQSSARKESRP
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA