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The map label for this gene is nuoF2 [H]

Identifier: 222524574

GI number: 222524574

Start: 1663795

End: 1665402

Strand: Direct

Name: nuoF2 [H]

Synonym: Chy400_1298

Alternate gene names: 222524574

Gene position: 1663795-1665402 (Clockwise)

Preceding gene: 222524573

Following gene: 222524575

Centisome position: 31.58

GC content: 56.03

Gene sequence:

>1608_bases
ATGGATCTTCACGAACTGGAAGCGATTGCTGAACGAGAACAGGCACAACGTGCGCGTTTCCGCATCCTCTGCTGCGCTGC
TGCCGGTTGCCAGGCTTCCGGCAGCCTGGAGATTAAGCGTCGCTTAGAACTGGTGCTCTCCGCTGCCGGCAAACAGGCTG
ATGTTGACGTTGTTCCGGTTGGGTGCATGGGATTGTGCGGTCACGGCCCGTTAGTTCGTCTGGAACCGACCGGAAAGGTG
TTCGAGCGCGTTACGCCGGCGGATGCCGAAGAGCTTGTCGCTGCGCTCGATCAGGGTTCGTGCAACGTGCAGCAATGTAA
CCTGAACCATCCTTTCTTCACTCGCCAGTATCCGATAGTCCTGCGTCACAGTGGTCGCATTAATCCCGAACGCATCGAGG
ACTATATCGCTGCGGGTGGCTACCGTATGCTCTACCACGTCATTCACGATCTGACACCGGCTGAGGTGATTCAGATGATT
ACGCGCAGTGGTCTGCGTGGACGTGGTGGGGCCGGCTATCCAACCGGGCTTAAGTGGGCCACTGTTGCCAAAAGCCCCGG
TGAGCGGAAGTTTGTGATTTGCAATGCCGATGAAGGTGATCCCGGTGCCTTTATGGATCGCAGCGTGCTTGAGAGCGATC
CGCATCTGGTGCTGGAAGGAATGGCAATCGCAGCCTACGCAGTCGGCGCCGAGCAAGGCTTTATTTATGTGCGCGGAGAG
TATCCGCTGGCAATCGAACGCCTGCAAAAAGCGATAGTGCAGGCGCGACGGTTAGGTCTCCTCGGTGCCCAGATTTTTGA
GAGTGGTTTCAACTTTCGGGTTGATATTCGCGTTGGTGCAGGGGCCTTTGTGTGTGGCGAAGAGACGGCCCTGATTGCCT
CGATTGAAGGTCGGCGCGGCCAACCCCGGCCACGTCCGCCGTATCCGGCAGAGAGTGGTCTGTGGGGAATGCCAACTCTG
ATCAACAACGTTGAGACCTTTGCCAACATTTCGGCCATTATCGAGCACGGCCCGGAATGGTTTCGCCAGATTGGTACCGA
ACAAAGCAAAGGAACAAAGGTCTTTGCATTGACCGGTAAGATTCGCAACACCGGTTTAATCGAGGTGCCGATGGGGATCA
CGCTCCGCGAGATTGTCGAAGACATTGGTGATGGTACCCCCGATGGAACCCCGGTCAAGGCAGTACAAACCGGTGGCCCG
TCAGGTGGCTGCATTCCGGCCAGCCTGTTTGATACGCCGGTTGATTATGAATCGCTGGCAGCGGTCGGCTCGATTATGGG
GTCGGGTGGGATGGTCGTGATGGACGAGCACACGAACATGGTTGATGTCGCTCGTTTCTATATGGAGTTTTGCAAAGATG
AGTCGTGCGGTAAGTGCATCCCATGTCGGGTAGGAACTGTCCAGCTCTTCAATCTACTCACCAAATTGCGCAATGGACAG
GCCGGCGAGAGTGATTTGCAAACGATTGAAGCATTATGCGAACTGATGCGTGAAACAAGCCTCTGTGGCCTGGGCCAATC
AGCGCCAAACCCGGTACTTAGCACCCTACACTACTTCCGACACGAATACGAACAGCTCATCGGAGCGGAGGTTGATCATG
GCGGCTAA

Upstream 100 bases:

>100_bases
TGCGCCTGCCGTCGTTCTTGATGGGGAAGTGGTTGGTCATGCTGATCGCTCCGACCTGAAAGCTCGCATTCAGGCGTTGC
TGAAACGCGAGGAGTAATGT

Downstream 100 bases:

>100_bases
AACACTCACAATTGATGGCCATCTCGTCTCGGCCCGCCCCGGTGCAACGCTATTGGAAGCTATCCGCGACGCTGGTATTC
CCATTCCTACCCTTTGTCAT

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MDLHELEAIAEREQAQRARFRILCCAAAGCQASGSLEIKRRLELVLSAAGKQADVDVVPVGCMGLCGHGPLVRLEPTGKV
FERVTPADAEELVAALDQGSCNVQQCNLNHPFFTRQYPIVLRHSGRINPERIEDYIAAGGYRMLYHVIHDLTPAEVIQMI
TRSGLRGRGGAGYPTGLKWATVAKSPGERKFVICNADEGDPGAFMDRSVLESDPHLVLEGMAIAAYAVGAEQGFIYVRGE
YPLAIERLQKAIVQARRLGLLGAQIFESGFNFRVDIRVGAGAFVCGEETALIASIEGRRGQPRPRPPYPAESGLWGMPTL
INNVETFANISAIIEHGPEWFRQIGTEQSKGTKVFALTGKIRNTGLIEVPMGITLREIVEDIGDGTPDGTPVKAVQTGGP
SGGCIPASLFDTPVDYESLAAVGSIMGSGGMVVMDEHTNMVDVARFYMEFCKDESCGKCIPCRVGTVQLFNLLTKLRNGQ
AGESDLQTIEALCELMRETSLCGLGQSAPNPVLSTLHYFRHEYEQLIGAEVDHGG

Sequences:

>Translated_535_residues
MDLHELEAIAEREQAQRARFRILCCAAAGCQASGSLEIKRRLELVLSAAGKQADVDVVPVGCMGLCGHGPLVRLEPTGKV
FERVTPADAEELVAALDQGSCNVQQCNLNHPFFTRQYPIVLRHSGRINPERIEDYIAAGGYRMLYHVIHDLTPAEVIQMI
TRSGLRGRGGAGYPTGLKWATVAKSPGERKFVICNADEGDPGAFMDRSVLESDPHLVLEGMAIAAYAVGAEQGFIYVRGE
YPLAIERLQKAIVQARRLGLLGAQIFESGFNFRVDIRVGAGAFVCGEETALIASIEGRRGQPRPRPPYPAESGLWGMPTL
INNVETFANISAIIEHGPEWFRQIGTEQSKGTKVFALTGKIRNTGLIEVPMGITLREIVEDIGDGTPDGTPVKAVQTGGP
SGGCIPASLFDTPVDYESLAAVGSIMGSGGMVVMDEHTNMVDVARFYMEFCKDESCGKCIPCRVGTVQLFNLLTKLRNGQ
AGESDLQTIEALCELMRETSLCGLGQSAPNPVLSTLHYFRHEYEQLIGAEVDHGG
>Mature_535_residues
MDLHELEAIAEREQAQRARFRILCCAAAGCQASGSLEIKRRLELVLSAAGKQADVDVVPVGCMGLCGHGPLVRLEPTGKV
FERVTPADAEELVAALDQGSCNVQQCNLNHPFFTRQYPIVLRHSGRINPERIEDYIAAGGYRMLYHVIHDLTPAEVIQMI
TRSGLRGRGGAGYPTGLKWATVAKSPGERKFVICNADEGDPGAFMDRSVLESDPHLVLEGMAIAAYAVGAEQGFIYVRGE
YPLAIERLQKAIVQARRLGLLGAQIFESGFNFRVDIRVGAGAFVCGEETALIASIEGRRGQPRPRPPYPAESGLWGMPTL
INNVETFANISAIIEHGPEWFRQIGTEQSKGTKVFALTGKIRNTGLIEVPMGITLREIVEDIGDGTPDGTPVKAVQTGGP
SGGCIPASLFDTPVDYESLAAVGSIMGSGGMVVMDEHTNMVDVARFYMEFCKDESCGKCIPCRVGTVQLFNLLTKLRNGQ
AGESDLQTIEALCELMRETSLCGLGQSAPNPVLSTLHYFRHEYEQLIGAEVDHGG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=376, Percent_Identity=39.6276595744681, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI260656005, Length=376, Percent_Identity=39.6276595744681, Blast_Score=281, Evalue=9e-76,
Organism=Escherichia coli, GI1788620, Length=410, Percent_Identity=40.4878048780488, Blast_Score=345, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI17531727, Length=451, Percent_Identity=35.6984478935698, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI45550452, Length=375, Percent_Identity=39.2, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI19920794, Length=373, Percent_Identity=37.5335120643432, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24653819, Length=378, Percent_Identity=30.6878306878307, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24653817, Length=353, Percent_Identity=28.8951841359773, Blast_Score=178, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57861; Mature: 57861

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHELEAIAEREQAQRARFRILCCAAAGCQASGSLEIKRRLELVLSAAGKQADVDVVPV
CCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
GCMGLCGHGPLVRLEPTGKVFERVTPADAEELVAALDQGSCNVQQCNLNHPFFTRQYPIV
CHHEECCCCCEEEECCCHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCEECCCCCEE
LRHSGRINPERIEDYIAAGGYRMLYHVIHDLTPAEVIQMITRSGLRGRGGAGYPTGLKWA
EECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TVAKSPGERKFVICNADEGDPGAFMDRSVLESDPHLVLEGMAIAAYAVGAEQGFIYVRGE
EECCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEHHHEEEHEEECCCCCEEEEECC
YPLAIERLQKAIVQARRLGLLGAQIFESGFNFRVDIRVGAGAFVCGEETALIASIEGRRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCEEEEEECCCCC
QPRPRPPYPAESGLWGMPTLINNVETFANISAIIEHGPEWFRQIGTEQSKGTKVFALTGK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECC
IRNTGLIEVPMGITLREIVEDIGDGTPDGTPVKAVQTGGPSGGCIPASLFDTPVDYESLA
CCCCCEEEECCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCCHHHHH
AVGSIMGSGGMVVMDEHTNMVDVARFYMEFCKDESCGKCIPCRVGTVQLFNLLTKLRNGQ
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
AGESDLQTIEALCELMRETSLCGLGQSAPNPVLSTLHYFRHEYEQLIGAEVDHGG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDLHELEAIAEREQAQRARFRILCCAAAGCQASGSLEIKRRLELVLSAAGKQADVDVVPV
CCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
GCMGLCGHGPLVRLEPTGKVFERVTPADAEELVAALDQGSCNVQQCNLNHPFFTRQYPIV
CHHEECCCCCEEEECCCHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCEECCCCCEE
LRHSGRINPERIEDYIAAGGYRMLYHVIHDLTPAEVIQMITRSGLRGRGGAGYPTGLKWA
EECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TVAKSPGERKFVICNADEGDPGAFMDRSVLESDPHLVLEGMAIAAYAVGAEQGFIYVRGE
EECCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEHHHEEEHEEECCCCCEEEEECC
YPLAIERLQKAIVQARRLGLLGAQIFESGFNFRVDIRVGAGAFVCGEETALIASIEGRRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCEEEEEECCCCC
QPRPRPPYPAESGLWGMPTLINNVETFANISAIIEHGPEWFRQIGTEQSKGTKVFALTGK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECC
IRNTGLIEVPMGITLREIVEDIGDGTPDGTPVKAVQTGGPSGGCIPASLFDTPVDYESLA
CCCCCEEEECCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCCHHHHH
AVGSIMGSGGMVVMDEHTNMVDVARFYMEFCKDESCGKCIPCRVGTVQLFNLLTKLRNGQ
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
AGESDLQTIEALCELMRETSLCGLGQSAPNPVLSTLHYFRHEYEQLIGAEVDHGG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]