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The map label for this gene is ytcB [H]

Identifier: 222524557

GI number: 222524557

Start: 1640499

End: 1641443

Strand: Direct

Name: ytcB [H]

Synonym: Chy400_1281

Alternate gene names: 222524557

Gene position: 1640499-1641443 (Clockwise)

Preceding gene: 222524556

Following gene: 222524558

Centisome position: 31.14

GC content: 53.54

Gene sequence:

>945_bases
ATGCGCATCCTGATCACCGGTTCTTCAGGCATGATTGGTACCAACCTCGGTCTTCGGCTGATCGAGGCTGGTCACCAGGT
TTTTGGCGTTGATCGTCGGGTTAATCCGTGGACGGATCGCATTCCCACCCTGTTACAAGACCTCGCTATACCCCAACGTG
ATTTTCGCGCCGGTATCGGTGGTGCCCCCTACCCACCTTGCGATCTGGTGGTGCATCTGGCGGCCAACGCCAAAGTCTAC
GAGCTGGTAACCGAGCCACATCGCGCCCTGGAAAATATCAACATTACCTATAATGTGCTCGAATATTGCCGGCATCATGA
AATCCCGATCATCTTTGCCTCCTCACGGGAAGTCTACGGTGACATTCACCGCTACATGACAGAGGAGACGCGCGCCGACT
TCAGCTTTACCGAGAGTCCCTACTCAGCTTCCAAAATTGCCGGTGAAGCCCTGATCTATGCCTATGCCCGCTGCTATGGG
CTGCGCTACATTGTCTTTCGCTTTTCCAATGTCTACGGCCGCTTCGATAACGATCTGGCACGCATGGAACGGGTGATTCC
GCTATTTATTCATCGGATCAGTCGTGGGGAACCGGTGACGGTATACGGTGCCGATAAGGTGCTTGATTTTACCTACATTG
ACGATTGTGTTGATGGAATTATGTGCGGTATCAACCGTTTATTCCGAGGGGAGGTATACAACCGCACAATTAATCTGGCC
TACGGCGAAGGAAATACGTTGATTCGGGTCGCGGAGTTGATCGGTGAGGCCTTTGGTCGGCAGCCGGAGATTATTGTTCA
GCCGGCAAAGCGGGTCGGTGAAGTAACGTACTATGTCGCTGATATTCGGCAGGCCCGCGAATTGTTGGGATTCAACCCGC
AAGTACCGTTAGCGGAAGGTATCAACCGCGCCGTGGCCTGGTCGCACGGAGAGTTGCTACGATGA

Upstream 100 bases:

>100_bases
ACCCAAACGCCAAACCGCTTCCGGTGATAGCGTTTGGGTTTTGCAACAATGATGTATCGGTAGCAACTTCCTGATCCAGT
TTGGCGAGACGGTTCTATCA

Downstream 100 bases:

>100_bases
CTGAACACCAGCCAGAGCTTAGCGCATCTGATCAGATGATAGAGCGAGCAGTGTCAGGTGAGGGTATCCAGCACTGGCTC
AAGATCAGTGCGGCCTGGCT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRILITGSSGMIGTNLGLRLIEAGHQVFGVDRRVNPWTDRIPTLLQDLAIPQRDFRAGIGGAPYPPCDLVVHLAANAKVY
ELVTEPHRALENINITYNVLEYCRHHEIPIIFASSREVYGDIHRYMTEETRADFSFTESPYSASKIAGEALIYAYARCYG
LRYIVFRFSNVYGRFDNDLARMERVIPLFIHRISRGEPVTVYGADKVLDFTYIDDCVDGIMCGINRLFRGEVYNRTINLA
YGEGNTLIRVAELIGEAFGRQPEIIVQPAKRVGEVTYYVADIRQARELLGFNPQVPLAEGINRAVAWSHGELLR

Sequences:

>Translated_314_residues
MRILITGSSGMIGTNLGLRLIEAGHQVFGVDRRVNPWTDRIPTLLQDLAIPQRDFRAGIGGAPYPPCDLVVHLAANAKVY
ELVTEPHRALENINITYNVLEYCRHHEIPIIFASSREVYGDIHRYMTEETRADFSFTESPYSASKIAGEALIYAYARCYG
LRYIVFRFSNVYGRFDNDLARMERVIPLFIHRISRGEPVTVYGADKVLDFTYIDDCVDGIMCGINRLFRGEVYNRTINLA
YGEGNTLIRVAELIGEAFGRQPEIIVQPAKRVGEVTYYVADIRQARELLGFNPQVPLAEGINRAVAWSHGELLR
>Mature_314_residues
MRILITGSSGMIGTNLGLRLIEAGHQVFGVDRRVNPWTDRIPTLLQDLAIPQRDFRAGIGGAPYPPCDLVVHLAANAKVY
ELVTEPHRALENINITYNVLEYCRHHEIPIIFASSREVYGDIHRYMTEETRADFSFTESPYSASKIAGEALIYAYARCYG
LRYIVFRFSNVYGRFDNDLARMERVIPLFIHRISRGEPVTVYGADKVLDFTYIDDCVDGIMCGINRLFRGEVYNRTINLA
YGEGNTLIRVAELIGEAFGRQPEIIVQPAKRVGEVTYYVADIRQARELLGFNPQVPLAEGINRAVAWSHGELLR

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=29.585798816568, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=29.3255131964809, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI48994969, Length=337, Percent_Identity=28.7833827893175, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=323, Percent_Identity=26.625386996904, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=254, Percent_Identity=27.5590551181102, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=23.4042553191489, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=29.9093655589124, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 35534; Mature: 35534

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILITGSSGMIGTNLGLRLIEAGHQVFGVDRRVNPWTDRIPTLLQDLAIPQRDFRAGIG
CEEEEECCCCCEECCCCHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCCC
GAPYPPCDLVVHLAANAKVYELVTEPHRALENINITYNVLEYCRHHEIPIIFASSREVYG
CCCCCHHHHEEECCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCHHHHH
DIHRYMTEETRADFSFTESPYSASKIAGEALIYAYARCYGLRYIVFRFSNVYGRFDNDLA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RMERVIPLFIHRISRGEPVTVYGADKVLDFTYIDDCVDGIMCGINRLFRGEVYNRTINLA
HHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEE
YGEGNTLIRVAELIGEAFGRQPEIIVQPAKRVGEVTYYVADIRQARELLGFNPQVPLAEG
ECCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
INRAVAWSHGELLR
CCHHEEECCCCCCC
>Mature Secondary Structure
MRILITGSSGMIGTNLGLRLIEAGHQVFGVDRRVNPWTDRIPTLLQDLAIPQRDFRAGIG
CEEEEECCCCCEECCCCHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCCC
GAPYPPCDLVVHLAANAKVYELVTEPHRALENINITYNVLEYCRHHEIPIIFASSREVYG
CCCCCHHHHEEECCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCHHHHH
DIHRYMTEETRADFSFTESPYSASKIAGEALIYAYARCYGLRYIVFRFSNVYGRFDNDLA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RMERVIPLFIHRISRGEPVTVYGADKVLDFTYIDDCVDGIMCGINRLFRGEVYNRTINLA
HHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEE
YGEGNTLIRVAELIGEAFGRQPEIIVQPAKRVGEVTYYVADIRQARELLGFNPQVPLAEG
ECCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
INRAVAWSHGELLR
CCHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]