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The map label for this gene is carA [H]

Identifier: 222524217

GI number: 222524217

Start: 1182680

End: 1183804

Strand: Reverse

Name: carA [H]

Synonym: Chy400_0938

Alternate gene names: 222524217

Gene position: 1183804-1182680 (Counterclockwise)

Preceding gene: 222524218

Following gene: 222524216

Centisome position: 22.47

GC content: 59.47

Gene sequence:

>1125_bases
ATGGATGCAGTGCTTGTCTTAGAAGATGGTCGCGTCTTTCCCGGGCGGTCGTTCGGTGCCCCGGGTGAGCGTGTTGGTGA
GGTTGTTTTTCACACCGGCATGACCGGCTATCAAGAGATTTTGACCGATCCCTCGTACTGCGGTCAACTGGTCACGATGA
CAGCACCGCACATTGGGAACACCGGCGTCAACGATTTTGACCCGGAGTCGATCCAGCCGCAGGTTGCCGGTTTTATTGTG
CGTAGCTACAGCGAACACTACAGCTCATGGCGTGCGCGTGGCAGTCTCTCTCAACTGCTGCGCGACTATGGGATCGTGGC
GATTAGCGATGTTGATACCCGTGCCCTCACTCGCCATATCCGTACCGCCGGGGCGATGCGCGGCATTATCTCCACAACCG
GCGAGTCGATAGAGTCGTTACTCGCGAAATGTCGGGCCGCGCCGCCGATGGAAGGGTTGGATTTAACGCAAGTGGTGACC
TGTCCCGAACCGTACCATTGGGCAACCAACAGTGTGCCGTTTACCCCGCCCTACCCGAACGGCCCGGCGCAAGAACTGCG
CTTCCACGTTGTTGCTTACGACTTCGGCCTCAAGCGCACCATTTTGCGCCGTCTGGTCGATCATGGCTGTCGGGTGACGG
TGGTACCGGCCACAACTCCACCCGAAGATGCGCTGGCCCTCCAACCCGACGGTATCTTCTTCTCGAATGGGCCGGGCGAT
CCGGCAGCGGCGACCTATGCTGTGGCCACGCTGCGCTCGCTGCTCGGTAAACTCCCGATCTTTGGCATCTGTCTCGGCCA
TCAATTGATGGGGCTGGCCCTCGGCGGGCGCACCTACAAGTTGCCGTTCGGCCACCACGGTGCCAACCATCCGGTGCGCT
ATCTGCCCACCGGTCGGGTTGAGATCACCTCGCAGAATCATGGGTTTGCCGTCGATCCGGACTCGTTGCCGGCAGGTGTT
GTTTTGACCCATATCAACCTGAATGATCAGACCCTGGAGGGTTTTGATTATCCTGAATTGCAGTGCTTCAGTGTGCAGTA
CCACCCCGAAGCAGGGCCAGGGCCACACGATGCAACCTATCTCTTTGCCCGCTTTGCCGAGGCAATGGCTGCCCGCCGTT
CGTAG

Upstream 100 bases:

>100_bases
ACGATCCAACGAACAACGTAACTTCTATGTTACAATACGCCAAATAGTTGGATGGGCGGCAAGAACTACGCCGCGTCTCA
CAATGAGAGTACAACAGACA

Downstream 100 bases:

>100_bases
GCGGAAGCAGGCCGGGAAGGTGGCCGGGGGAGAGACGGCCACCTTCTGCGTTACCCATGCCTGCGCCAGACCGAATCAAG
ACACCGTGCAGATGTAGAGC

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MDAVLVLEDGRVFPGRSFGAPGERVGEVVFHTGMTGYQEILTDPSYCGQLVTMTAPHIGNTGVNDFDPESIQPQVAGFIV
RSYSEHYSSWRARGSLSQLLRDYGIVAISDVDTRALTRHIRTAGAMRGIISTTGESIESLLAKCRAAPPMEGLDLTQVVT
CPEPYHWATNSVPFTPPYPNGPAQELRFHVVAYDFGLKRTILRRLVDHGCRVTVVPATTPPEDALALQPDGIFFSNGPGD
PAAATYAVATLRSLLGKLPIFGICLGHQLMGLALGGRTYKLPFGHHGANHPVRYLPTGRVEITSQNHGFAVDPDSLPAGV
VLTHINLNDQTLEGFDYPELQCFSVQYHPEAGPGPHDATYLFARFAEAMAARRS

Sequences:

>Translated_374_residues
MDAVLVLEDGRVFPGRSFGAPGERVGEVVFHTGMTGYQEILTDPSYCGQLVTMTAPHIGNTGVNDFDPESIQPQVAGFIV
RSYSEHYSSWRARGSLSQLLRDYGIVAISDVDTRALTRHIRTAGAMRGIISTTGESIESLLAKCRAAPPMEGLDLTQVVT
CPEPYHWATNSVPFTPPYPNGPAQELRFHVVAYDFGLKRTILRRLVDHGCRVTVVPATTPPEDALALQPDGIFFSNGPGD
PAAATYAVATLRSLLGKLPIFGICLGHQLMGLALGGRTYKLPFGHHGANHPVRYLPTGRVEITSQNHGFAVDPDSLPAGV
VLTHINLNDQTLEGFDYPELQCFSVQYHPEAGPGPHDATYLFARFAEAMAARRS
>Mature_374_residues
MDAVLVLEDGRVFPGRSFGAPGERVGEVVFHTGMTGYQEILTDPSYCGQLVTMTAPHIGNTGVNDFDPESIQPQVAGFIV
RSYSEHYSSWRARGSLSQLLRDYGIVAISDVDTRALTRHIRTAGAMRGIISTTGESIESLLAKCRAAPPMEGLDLTQVVT
CPEPYHWATNSVPFTPPYPNGPAQELRFHVVAYDFGLKRTILRRLVDHGCRVTVVPATTPPEDALALQPDGIFFSNGPGD
PAAATYAVATLRSLLGKLPIFGICLGHQLMGLALGGRTYKLPFGHHGANHPVRYLPTGRVEITSQNHGFAVDPDSLPAGV
VLTHINLNDQTLEGFDYPELQCFSVQYHPEAGPGPHDATYLFARFAEAMAARRS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=380, Percent_Identity=40.5263157894737, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI169790915, Length=376, Percent_Identity=38.2978723404255, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI21361331, Length=374, Percent_Identity=38.2352941176471, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=52.9255319148936, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1789760, Length=135, Percent_Identity=32.5925925925926, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=391, Percent_Identity=40.153452685422, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=38.9873417721519, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=382, Percent_Identity=39.5287958115183, Blast_Score=235, Evalue=8e-63,
Organism=Drosophila melanogaster, GI45555749, Length=392, Percent_Identity=39.030612244898, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24642586, Length=392, Percent_Identity=39.030612244898, Blast_Score=235, Evalue=4e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40550; Mature: 40550

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVLVLEDGRVFPGRSFGAPGERVGEVVFHTGMTGYQEILTDPSYCGQLVTMTAPHIGN
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHHHCEEEEEECCCCCC
TGVNDFDPESIQPQVAGFIVRSYSEHYSSWRARGSLSQLLRDYGIVAISDVDTRALTRHI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEECCCHHHHHHHH
RTAGAMRGIISTTGESIESLLAKCRAAPPMEGLDLTQVVTCPEPYHWATNSVPFTPPYPN
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
GPAQELRFHVVAYDFGLKRTILRRLVDHGCRVTVVPATTPPEDALALQPDGIFFSNGPGD
CCHHHEEEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCEEEECCCCC
PAAATYAVATLRSLLGKLPIFGICLGHQLMGLALGGRTYKLPFGHHGANHPVRYLPTGRV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCEEEECCCCCCCCCCCEEEECCCEE
EITSQNHGFAVDPDSLPAGVVLTHINLNDQTLEGFDYPELQCFSVQYHPEAGPGPHDATY
EEEECCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHH
LFARFAEAMAARRS
HHHHHHHHHHHCCC
>Mature Secondary Structure
MDAVLVLEDGRVFPGRSFGAPGERVGEVVFHTGMTGYQEILTDPSYCGQLVTMTAPHIGN
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHHHCEEEEEECCCCCC
TGVNDFDPESIQPQVAGFIVRSYSEHYSSWRARGSLSQLLRDYGIVAISDVDTRALTRHI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEECCCHHHHHHHH
RTAGAMRGIISTTGESIESLLAKCRAAPPMEGLDLTQVVTCPEPYHWATNSVPFTPPYPN
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
GPAQELRFHVVAYDFGLKRTILRRLVDHGCRVTVVPATTPPEDALALQPDGIFFSNGPGD
CCHHHEEEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCEEEECCCCC
PAAATYAVATLRSLLGKLPIFGICLGHQLMGLALGGRTYKLPFGHHGANHPVRYLPTGRV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCEEEECCCCCCCCCCCEEEECCCEE
EITSQNHGFAVDPDSLPAGVVLTHINLNDQTLEGFDYPELQCFSVQYHPEAGPGPHDATY
EEEECCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHH
LFARFAEAMAARRS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA