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The map label for this gene is sir [H]

Identifier: 222524027

GI number: 222524027

Start: 926753

End: 928426

Strand: Reverse

Name: sir [H]

Synonym: Chy400_0742

Alternate gene names: 222524027

Gene position: 928426-926753 (Counterclockwise)

Preceding gene: 222524028

Following gene: 222524023

Centisome position: 17.62

GC content: 56.09

Gene sequence:

>1674_bases
ATGACGGATACACCCAGGCTTTCAAAAGTTGAGCAGATCAAACTGGCCAGCCGGCATTTGCGAGGGCCGGTAGATCACGA
GCTGCACGACGGAAACGATCACTTCAGTGATGAAGGGTATCAGATTCTCAAGTTTCACGGCATTTATCAACAAGACAATC
GCGATGTGCGTAAAGCCCGCAAAGCGCGGGGATTGGGGCCGGAGTATTCATTTATGATCCGGGTGGCTATTCCCGGTGGC
CGGCTTAGCCCGGAACAGTATCTGACCCTCGACCGGCTGGCGGATGAGTTGGGCAATACCACGTTGCGTTTTACTACTCG
CCAGGCCATTCAGTACCACGGCGTGCGCAAGGGTGGCCTGAAGCCACTGATTCGCACGCTTAACCGTCATCTGCTGACTA
CACTCTCGGCATGTGGTGATGTGGTGCGGAACATTGTGGCCTGCCCTGCACCTTTTGTTGATCGTCAGCGGATCGAGCTG
ATGCAGTATGCGAAAGCTCTTTCTGATCGGCTGAAGCCAAAGACCCGTGCCTACTACGAAATCTGGCTCGATGGGGAAAA
GGCGGCGAGTCTGGAAGAGGATGAACCGCTCTACGGTGCAACCTATCTGCCGCGCAAGTTCAAGATTGCCTTTGCCTTCC
CCGGCGATAACTGTGTTGATGTCTATACCCAGGACATTGGAATTGTGCCGTGGATTGAAGAGAGTCTGCAAGGGTTCACC
ATTCTGGTTGGTGGTGGTCTGGGCCAGAGTCATGGGGTTAAGGAAACCCATCCGGCACTGGCAAAGCCGCTTACCACTGT
AGCTCCCGCCCAGCTTTTTGAGGTTGTGGAAGCGATTGTGAAGGTGCAACGCGATCATGGCCGACGTGATGACCGCAAGC
TGGCCCGGATGAAATATCTGGTTGAGAGTTGGGGCATCGAGCGGTTCAAAGCTGAGGTCGAGCGCTACGTTGGCTATGCG
TTGCCTGAACCCAGACCGCTCACGTGGCTCAGCGCCGATGATCACCTGGGATGGCACGAACAGGGTGATGGCCGGCTCTT
CTTTGGCCTGTTTGTCGAAAATGGGCGGGTCAGAGATCATCTCCGGGCAGCAATTCGGGATGTTGTGCAGCGTTTCGGCC
TCGAAGTTCGCCTGACTCCGCAGCAGAATATCCTGTTCGTGAATATTCAACCGGCTGATCGTGCGGCCATCGAGGCAATC
TTTCGCCAGTATGATGTCGCGCTACCCGGTACCATCCCGCTCGTTGTTCAGAATGCGCTGGCCTGTCCAGCTCTCCCCAC
CTGTGGTCTGGCAATTACCGAAAGCGAGCGGGTCATGCCGCAGGTGATTCGTGACATGAACGCCCTGCTGGATCGGCTGG
GACTCCACGATGGCCCGGTTCCGCACGTCAGAATGACCGGCTGCCCCAATGGGTGTGCCCGACCCTATACCGCTGAAATC
GGATTGGTAGGACGCAGCCTGAATTCTTACACCATTTATCTGGGTGGCAGTCCTTTGGGAACCCGCCTGGGAGAAGTCTA
TCTCGATAATGTCAAGCGCGAGGAGATTGTCGCGCGGCTGGAACCGATATTGCTACTCTATCAGCGCGAACGCAACCCGG
GCGAGTCCTTTGGTGATTTCTGCCATCGGCTGGGCAGTGCAACCTTGCAACACCTTGCCGGGCAGAACGAATAA

Upstream 100 bases:

>100_bases
AATGCCTGTTCCGGCTTACACCGGGATGCATCAGGCCAGGGACGTGGTCGTTGTTGAACGCGGATAGCAATCCGTGAAGC
TATGAGTGCGGAGAAAGTTT

Downstream 100 bases:

>100_bases
CAAACCATCGGGGGTAACAGCAATGCGTTACCCCCGGTTCCTGACGGATATGGTGTTGTTGTGCGGATATTTGTCAACCG
ACCATCTCACGAGCGGAAGA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MTDTPRLSKVEQIKLASRHLRGPVDHELHDGNDHFSDEGYQILKFHGIYQQDNRDVRKARKARGLGPEYSFMIRVAIPGG
RLSPEQYLTLDRLADELGNTTLRFTTRQAIQYHGVRKGGLKPLIRTLNRHLLTTLSACGDVVRNIVACPAPFVDRQRIEL
MQYAKALSDRLKPKTRAYYEIWLDGEKAASLEEDEPLYGATYLPRKFKIAFAFPGDNCVDVYTQDIGIVPWIEESLQGFT
ILVGGGLGQSHGVKETHPALAKPLTTVAPAQLFEVVEAIVKVQRDHGRRDDRKLARMKYLVESWGIERFKAEVERYVGYA
LPEPRPLTWLSADDHLGWHEQGDGRLFFGLFVENGRVRDHLRAAIRDVVQRFGLEVRLTPQQNILFVNIQPADRAAIEAI
FRQYDVALPGTIPLVVQNALACPALPTCGLAITESERVMPQVIRDMNALLDRLGLHDGPVPHVRMTGCPNGCARPYTAEI
GLVGRSLNSYTIYLGGSPLGTRLGEVYLDNVKREEIVARLEPILLLYQRERNPGESFGDFCHRLGSATLQHLAGQNE

Sequences:

>Translated_557_residues
MTDTPRLSKVEQIKLASRHLRGPVDHELHDGNDHFSDEGYQILKFHGIYQQDNRDVRKARKARGLGPEYSFMIRVAIPGG
RLSPEQYLTLDRLADELGNTTLRFTTRQAIQYHGVRKGGLKPLIRTLNRHLLTTLSACGDVVRNIVACPAPFVDRQRIEL
MQYAKALSDRLKPKTRAYYEIWLDGEKAASLEEDEPLYGATYLPRKFKIAFAFPGDNCVDVYTQDIGIVPWIEESLQGFT
ILVGGGLGQSHGVKETHPALAKPLTTVAPAQLFEVVEAIVKVQRDHGRRDDRKLARMKYLVESWGIERFKAEVERYVGYA
LPEPRPLTWLSADDHLGWHEQGDGRLFFGLFVENGRVRDHLRAAIRDVVQRFGLEVRLTPQQNILFVNIQPADRAAIEAI
FRQYDVALPGTIPLVVQNALACPALPTCGLAITESERVMPQVIRDMNALLDRLGLHDGPVPHVRMTGCPNGCARPYTAEI
GLVGRSLNSYTIYLGGSPLGTRLGEVYLDNVKREEIVARLEPILLLYQRERNPGESFGDFCHRLGSATLQHLAGQNE
>Mature_556_residues
TDTPRLSKVEQIKLASRHLRGPVDHELHDGNDHFSDEGYQILKFHGIYQQDNRDVRKARKARGLGPEYSFMIRVAIPGGR
LSPEQYLTLDRLADELGNTTLRFTTRQAIQYHGVRKGGLKPLIRTLNRHLLTTLSACGDVVRNIVACPAPFVDRQRIELM
QYAKALSDRLKPKTRAYYEIWLDGEKAASLEEDEPLYGATYLPRKFKIAFAFPGDNCVDVYTQDIGIVPWIEESLQGFTI
LVGGGLGQSHGVKETHPALAKPLTTVAPAQLFEVVEAIVKVQRDHGRRDDRKLARMKYLVESWGIERFKAEVERYVGYAL
PEPRPLTWLSADDHLGWHEQGDGRLFFGLFVENGRVRDHLRAAIRDVVQRFGLEVRLTPQQNILFVNIQPADRAAIEAIF
RQYDVALPGTIPLVVQNALACPALPTCGLAITESERVMPQVIRDMNALLDRLGLHDGPVPHVRMTGCPNGCARPYTAEIG
LVGRSLNSYTIYLGGSPLGTRLGEVYLDNVKREEIVARLEPILLLYQRERNPGESFGDFCHRLGSATLQHLAGQNE

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=549, Percent_Identity=37.1584699453552, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6322597, Length=583, Percent_Identity=40.1372212692967, Blast_Score=417, Evalue=1e-117,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62618; Mature: 62486

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTPRLSKVEQIKLASRHLRGPVDHELHDGNDHFSDEGYQILKFHGIYQQDNRDVRKAR
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHH
KARGLGPEYSFMIRVAIPGGRLSPEQYLTLDRLADELGNTTLRFTTRQAIQYHGVRKGGL
HHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCH
KPLIRTLNRHLLTTLSACGDVVRNIVACPAPFVDRQRIELMQYAKALSDRLKPKTRAYYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IWLDGEKAASLEEDEPLYGATYLPRKFKIAFAFPGDNCVDVYTQDIGIVPWIEESLQGFT
EEECCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCEEEEECCCCCCCCCHHHCCCCEE
ILVGGGLGQSHGVKETHPALAKPLTTVAPAQLFEVVEAIVKVQRDHGRRDDRKLARMKYL
EEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VESWGIERFKAEVERYVGYALPEPRPLTWLSADDHLGWHEQGDGRLFFGLFVENGRVRDH
HHHHCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHH
LRAAIRDVVQRFGLEVRLTPQQNILFVNIQPADRAAIEAIFRQYDVALPGTIPLVVQNAL
HHHHHHHHHHHCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ACPALPTCGLAITESERVMPQVIRDMNALLDRLGLHDGPVPHVRMTGCPNGCARPYTAEI
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEH
GLVGRSLNSYTIYLGGSPLGTRLGEVYLDNVKREEIVARLEPILLLYQRERNPGESFGDF
EEEECCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCHHHHHH
CHRLGSATLQHLAGQNE
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDTPRLSKVEQIKLASRHLRGPVDHELHDGNDHFSDEGYQILKFHGIYQQDNRDVRKAR
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHH
KARGLGPEYSFMIRVAIPGGRLSPEQYLTLDRLADELGNTTLRFTTRQAIQYHGVRKGGL
HHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCH
KPLIRTLNRHLLTTLSACGDVVRNIVACPAPFVDRQRIELMQYAKALSDRLKPKTRAYYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IWLDGEKAASLEEDEPLYGATYLPRKFKIAFAFPGDNCVDVYTQDIGIVPWIEESLQGFT
EEECCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCEEEEECCCCCCCCCHHHCCCCEE
ILVGGGLGQSHGVKETHPALAKPLTTVAPAQLFEVVEAIVKVQRDHGRRDDRKLARMKYL
EEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VESWGIERFKAEVERYVGYALPEPRPLTWLSADDHLGWHEQGDGRLFFGLFVENGRVRDH
HHHHCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHH
LRAAIRDVVQRFGLEVRLTPQQNILFVNIQPADRAAIEAIFRQYDVALPGTIPLVVQNAL
HHHHHHHHHHHCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ACPALPTCGLAITESERVMPQVIRDMNALLDRLGLHDGPVPHVRMTGCPNGCARPYTAEI
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCEEEH
GLVGRSLNSYTIYLGGSPLGTRLGEVYLDNVKREEIVARLEPILLLYQRERNPGESFGDF
EEEECCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCHHHHHH
CHRLGSATLQHLAGQNE
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8347657 [H]