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The map label for this gene is cobA [H]

Identifier: 222524028

GI number: 222524028

Start: 928460

End: 929266

Strand: Reverse

Name: cobA [H]

Synonym: Chy400_0743

Alternate gene names: 222524028

Gene position: 929266-928460 (Counterclockwise)

Preceding gene: 222524029

Following gene: 222524027

Centisome position: 17.64

GC content: 60.1

Gene sequence:

>807_bases
ATGAGCAAGGTTTACCTGGTGGGTGCCGGCCCTGGTGATCCTGAATTACTTACGCTCCGTGCGCTACGGGTGCTCGGCGA
AGCTGATGTGGTGCTGTACGACCGCCTGGTGTCTGCGGAGATTCTGGCGTATGTCAACCCGCAAGCTGAACGCATCTACG
TCGGCAAAGCAAAGGGTGACCAGGATCGGGTGCAACGTGAGATTCTTCATCTGTTGTTGCACTATGCTCGCGCCGGACGC
ACGGTCGTGCGCTTGAAGGGCGGCGATCCCATGGTCTACGGGCGTGGTGCTGAAGAGTGGCTCTTTCTGGCTCAACACGG
CATTACCGTTGAGTTAGTGCCCGGTCTCAGTTCAGCGCTGGCGTTACCGGGAATGGCCGGTATTCCGTTGACATTACGCG
GTATTGGGCGAAGTTTTGCCGTGATCTGTGGTCAGGAGCAGGGTGGTGCTGCGCCTGAGATAGCTCCCTATGCCCGGATC
GATACTCTGGTGATTCTGATGGGGGTTGAACGGCGGGCAGAACTGGCGCAGGCGCTGATCCAGGCCGGTCGTGACGCCTG
GGAACCCTGCGCTTTTATCGAGAACGGCTCTACACCCAACGAACGTATTGTGCTGGCAGCGCTGGAAGCTGTGGCGCAGG
GGCGGGTTGCGGTTCAGGCACCGGCAGTCTGGGTAATCGGCCCGGTGGTGGCGGTGCGCGAGCAATTGCAGGCGGTGGCT
GCTGATGAACCGGTACATGCAATGCCTGTTCCGGCTTACACCGGGATGCATCAGGCCAGGGACGTGGTCGTTGTTGAACG
CGGATAG

Upstream 100 bases:

>100_bases
CAGGCGATTACGTTCAGGCGCAGAACCTGCTCTGGCAGGCTCTGTACGACACTGCTTCCGCTGTACCGGTCGATCTCAGC
CTGCATCAACAGGAGGTGGT

Downstream 100 bases:

>100_bases
CAATCCGTGAAGCTATGAGTGCGGAGAAAGTTTATGACGGATACACCCAGGCTTTCAAAAGTTGAGCAGATCAAACTGGC
CAGCCGGCATTTGCGAGGGC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSKVYLVGAGPGDPELLTLRALRVLGEADVVLYDRLVSAEILAYVNPQAERIYVGKAKGDQDRVQREILHLLLHYARAGR
TVVRLKGGDPMVYGRGAEEWLFLAQHGITVELVPGLSSALALPGMAGIPLTLRGIGRSFAVICGQEQGGAAPEIAPYARI
DTLVILMGVERRAELAQALIQAGRDAWEPCAFIENGSTPNERIVLAALEAVAQGRVAVQAPAVWVIGPVVAVREQLQAVA
ADEPVHAMPVPAYTGMHQARDVVVVERG

Sequences:

>Translated_268_residues
MSKVYLVGAGPGDPELLTLRALRVLGEADVVLYDRLVSAEILAYVNPQAERIYVGKAKGDQDRVQREILHLLLHYARAGR
TVVRLKGGDPMVYGRGAEEWLFLAQHGITVELVPGLSSALALPGMAGIPLTLRGIGRSFAVICGQEQGGAAPEIAPYARI
DTLVILMGVERRAELAQALIQAGRDAWEPCAFIENGSTPNERIVLAALEAVAQGRVAVQAPAVWVIGPVVAVREQLQAVA
ADEPVHAMPVPAYTGMHQARDVVVVERG
>Mature_267_residues
SKVYLVGAGPGDPELLTLRALRVLGEADVVLYDRLVSAEILAYVNPQAERIYVGKAKGDQDRVQREILHLLLHYARAGRT
VVRLKGGDPMVYGRGAEEWLFLAQHGITVELVPGLSSALALPGMAGIPLTLRGIGRSFAVICGQEQGGAAPEIAPYARID
TLVILMGVERRAELAQALIQAGRDAWEPCAFIENGSTPNERIVLAALEAVAQGRVAVQAPAVWVIGPVVAVREQLQAVAA
DEPVHAMPVPAYTGMHQARDVVVVERG

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=236, Percent_Identity=40.6779661016949, Blast_Score=166, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322922, Length=245, Percent_Identity=35.5102040816327, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 28642; Mature: 28510

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVYLVGAGPGDPELLTLRALRVLGEADVVLYDRLVSAEILAYVNPQAERIYVGKAKGD
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCEEEEECCCCC
QDRVQREILHLLLHYARAGRTVVRLKGGDPMVYGRGAEEWLFLAQHGITVELVPGLSSAL
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHEEEECCCCEEEECCCCHHHH
ALPGMAGIPLTLRGIGRSFAVICGQEQGGAAPEIAPYARIDTLVILMGVERRAELAQALI
HCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHEEEEEECCHHHHHHHHHHH
QAGRDAWEPCAFIENGSTPNERIVLAALEAVAQGRVAVQAPAVWVIGPVVAVREQLQAVA
HCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCEEEEHHHHHHHHHHHHHHH
ADEPVHAMPVPAYTGMHQARDVVVVERG
CCCCCCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKVYLVGAGPGDPELLTLRALRVLGEADVVLYDRLVSAEILAYVNPQAERIYVGKAKGD
CEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCEEEEECCCCC
QDRVQREILHLLLHYARAGRTVVRLKGGDPMVYGRGAEEWLFLAQHGITVELVPGLSSAL
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHEEEECCCCEEEECCCCHHHH
ALPGMAGIPLTLRGIGRSFAVICGQEQGGAAPEIAPYARIDTLVILMGVERRAELAQALI
HCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHEEEEEECCHHHHHHHHHHH
QAGRDAWEPCAFIENGSTPNERIVLAALEAVAQGRVAVQAPAVWVIGPVVAVREQLQAVA
HCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCEEEEHHHHHHHHHHHHHHH
ADEPVHAMPVPAYTGMHQARDVVVVERG
CCCCCCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]