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The map label for this gene is prfA [H]

Identifier: 222523819

GI number: 222523819

Start: 628158

End: 629240

Strand: Reverse

Name: prfA [H]

Synonym: Chy400_0527

Alternate gene names: 222523819

Gene position: 629240-628158 (Counterclockwise)

Preceding gene: 222523820

Following gene: 222523818

Centisome position: 11.94

GC content: 53.65

Gene sequence:

>1083_bases
ATGTTTGATAAACTGGCTGCTGTTGCTGCACGTTATGATGAACTAACCGAGTTGATGGCTCAGCCAGAGGTAGCGACCAA
CGTTACTCTCTTGCAGCAGTATGCTCGCGAACAGCGTGAAATTGAGGATATTGTTAACGCTTATCGCGAATATCAGGCTA
CCCAACGCGCAATTGAGGAGGCTGAAGCCATGCTCGAAGATAGCGATCCTGAATTGCGTGCACTGGCTCAGGAAGAGCTT
GAGACGCAACGGAAGCGCCTGGCGAGTCTGGAAGAGCAGTTGAAGCTGTTGTTATTGCCACGTGATCCGAACGACTCAAA
AGATGTCATTATGGAGATTCGGCAGGGTGAAGGTGGCGATGAAGCAGCGCTGTTTGCTGCCGATCTCTTCCGCATGTACA
CCCGCTTTGCCGAGTCGCGCGGCTGGAAGGTTGAAGTTGATAGTCTGACCGAAAACGGTATCGGTGGTATCAAAGAGGTT
ATCTTCCAGATTCATGGTGAAGGTGCGTACAGCCAATTGAAATATGAGGGCGGAGTTCATCGTGTGCAGCGTGTACCGGC
CACCGAAGCCCGTGGCCGCATCCATACATCGACTGCAACGGTTGCCGTGCTGCCCGAAGTCGAAGAAACAGAGATCGAGA
TTAAACCTGAAGATTTACGAATTGATGTGTTTCGGAGTGCCGGTCACGGTGGACAGGGGGTCAACACCACCGACTCGGCG
GTGCGGATCGTCTACAAGCCCGGTACCCCCGAAGAGATTGTGGTTACCTGTCAGGATGGCCGATCACAGATTCAGAACCG
TGAGCGGGCGATGACGGTGCTGCGTGCCCGGTTGTACGCGCGCGAGCAAGAGAAACGACAGCGCGAAATCGGCGCTTCAC
GACTGGCGCAGGTCGGTAGTGGTGAGCGGGCAGAGAAGATTCGGACGTACAATTTCCCGCAAGATCGGATAACCGATCAC
CGGATCGGGCAAAACTTCTCAAACTTGCCGGCTGTGCTCGATGGTGAACTCGATAAGATTATTGAAGCGCTGATTATTTA
CGATAATGCCGAACGGTTGCGGGCGAGTGGGGTTAGCACCTGA

Upstream 100 bases:

>100_bases
CGCGCTTGGCCTGTAATTGAAAAAACTTCTCGATGGCACATACGCTGCACTGGCAGTGCGTATTGTGCCATCGGCCATCT
TAACGTAATGGAATTATTGT

Downstream 100 bases:

>100_bases
AGCGCCCTTTGCATTCCCACTGTTGGCTTCTGCACATTGGCCGCTTACGCCGTTCTCACCACCCTACTGAAGCAGGGTGG
TGTCTTTTTTTGCGTGACTC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MFDKLAAVAARYDELTELMAQPEVATNVTLLQQYAREQREIEDIVNAYREYQATQRAIEEAEAMLEDSDPELRALAQEEL
ETQRKRLASLEEQLKLLLLPRDPNDSKDVIMEIRQGEGGDEAALFAADLFRMYTRFAESRGWKVEVDSLTENGIGGIKEV
IFQIHGEGAYSQLKYEGGVHRVQRVPATEARGRIHTSTATVAVLPEVEETEIEIKPEDLRIDVFRSAGHGGQGVNTTDSA
VRIVYKPGTPEEIVVTCQDGRSQIQNRERAMTVLRARLYAREQEKRQREIGASRLAQVGSGERAEKIRTYNFPQDRITDH
RIGQNFSNLPAVLDGELDKIIEALIIYDNAERLRASGVST

Sequences:

>Translated_360_residues
MFDKLAAVAARYDELTELMAQPEVATNVTLLQQYAREQREIEDIVNAYREYQATQRAIEEAEAMLEDSDPELRALAQEEL
ETQRKRLASLEEQLKLLLLPRDPNDSKDVIMEIRQGEGGDEAALFAADLFRMYTRFAESRGWKVEVDSLTENGIGGIKEV
IFQIHGEGAYSQLKYEGGVHRVQRVPATEARGRIHTSTATVAVLPEVEETEIEIKPEDLRIDVFRSAGHGGQGVNTTDSA
VRIVYKPGTPEEIVVTCQDGRSQIQNRERAMTVLRARLYAREQEKRQREIGASRLAQVGSGERAEKIRTYNFPQDRITDH
RIGQNFSNLPAVLDGELDKIIEALIIYDNAERLRASGVST
>Mature_360_residues
MFDKLAAVAARYDELTELMAQPEVATNVTLLQQYAREQREIEDIVNAYREYQATQRAIEEAEAMLEDSDPELRALAQEEL
ETQRKRLASLEEQLKLLLLPRDPNDSKDVIMEIRQGEGGDEAALFAADLFRMYTRFAESRGWKVEVDSLTENGIGGIKEV
IFQIHGEGAYSQLKYEGGVHRVQRVPATEARGRIHTSTATVAVLPEVEETEIEIKPEDLRIDVFRSAGHGGQGVNTTDSA
VRIVYKPGTPEEIVVTCQDGRSQIQNRERAMTVLRARLYAREQEKRQREIGASRLAQVGSGERAEKIRTYNFPQDRITDH
RIGQNFSNLPAVLDGELDKIIEALIIYDNAERLRASGVST

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=301, Percent_Identity=45.1827242524917, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI34577120, Length=321, Percent_Identity=41.4330218068536, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=46.6321243523316, Blast_Score=185, Evalue=7e-47,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=48.4419263456091, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=38.2352941176471, Blast_Score=198, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=287, Percent_Identity=37.6306620209059, Blast_Score=172, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=333, Percent_Identity=42.042042042042, Blast_Score=246, Evalue=4e-66,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=42.5076452599388, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40586; Mature: 40586

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKLAAVAARYDELTELMAQPEVATNVTLLQQYAREQREIEDIVNAYREYQATQRAIEE
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAMLEDSDPELRALAQEELETQRKRLASLEEQLKLLLLPRDPNDSKDVIMEIRQGEGGD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCCC
EAALFAADLFRMYTRFAESRGWKVEVDSLTENGIGGIKEVIFQIHGEGAYSQLKYEGGVH
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHH
RVQRVPATEARGRIHTSTATVAVLPEVEETEIEIKPEDLRIDVFRSAGHGGQGVNTTDSA
HHHHCCCHHHCCEEEECCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCE
VRIVYKPGTPEEIVVTCQDGRSQIQNRERAMTVLRARLYAREQEKRQREIGASRLAQVGS
EEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GERAEKIRTYNFPQDRITDHRIGQNFSNLPAVLDGELDKIIEALIIYDNAERLRASGVST
CCHHHHHHCCCCCHHHCCHHHHCCCHHCCCHHHCCCHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MFDKLAAVAARYDELTELMAQPEVATNVTLLQQYAREQREIEDIVNAYREYQATQRAIEE
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAMLEDSDPELRALAQEELETQRKRLASLEEQLKLLLLPRDPNDSKDVIMEIRQGEGGD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCCC
EAALFAADLFRMYTRFAESRGWKVEVDSLTENGIGGIKEVIFQIHGEGAYSQLKYEGGVH
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHH
RVQRVPATEARGRIHTSTATVAVLPEVEETEIEIKPEDLRIDVFRSAGHGGQGVNTTDSA
HHHHCCCHHHCCEEEECCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCE
VRIVYKPGTPEEIVVTCQDGRSQIQNRERAMTVLRARLYAREQEKRQREIGASRLAQVGS
EEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GERAEKIRTYNFPQDRITDHRIGQNFSNLPAVLDGELDKIIEALIIYDNAERLRASGVST
CCHHHHHHCCCCCHHHCCHHHHCCCHHCCCHHHCCCHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA