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The map label for this gene is trmD

Identifier: 222523669

GI number: 222523669

Start: 442971

End: 443732

Strand: Reverse

Name: trmD

Synonym: Chy400_0375

Alternate gene names: 222523669

Gene position: 443732-442971 (Counterclockwise)

Preceding gene: 222523670

Following gene: 222523667

Centisome position: 8.42

GC content: 60.5

Gene sequence:

>762_bases
ATGCGGTTTGATATTTTGACCCTCTTTCCGGCCATGTTTCAGGGGCCGTTGACAGAGAGTATTCTCAAGCGTGCCCAACA
GGCCGGGCGAATTGAGATTCACTTGCACGATATTCGACAATGGACGACGGATCGTCATCGGACGGCTGACGATACGCCAT
ACGGTGGCGGCGCCGGCATGGTAATGAAGGCGGAGCCGCTGGCGGCGGCGATTCGGGCAGTGCGGGCTGCTGATGAGCGA
CCTGGGGTGACCATTTTGCTAACCCCTGACGGTGAACTGCTAACGCAACAGATTGTGCGCGAACTGGCTACCTTGCCGCG
GTTGCTCCTGGTGTGTGGACACTATGAAGGGATCGATGAACGAGTACGCGAGACGCTGATTGACCGCGAGCTGAGTATTG
GTGATTATGTGCTTACCGGTGGTGAGCTGGCGGCAATGGTGGTGGTTGATGCTGTGGCCCGGCTGGTGCCCGGTGTGATC
GATAGCGAGTCGATTGTCGAGGAGAGTCACAGCGATTTTTTGCTCGAATATCCGCACTATACGCGCCCGGCAGTGTGGGA
GGGACGGGCAGTGCCACCCGTGCTTCTGTCTGGTCACCACGGTGAAATTGCCCGCTGGCGACGGGCCGAACGGTTACGGC
GCACGCTGGTCCGTCGTCCGGATCTGCTGGCGCGGGCGGCGGCTGCCGGTGTGCTGACCAAAGCCGATCTGGCACTGCTG
GCCGAGTTGGGCTGGCGTCCTGAAACCTCCAACGGCGCGTAG

Upstream 100 bases:

>100_bases
CTGAACACTGTGGCGGTTATTTTGTTGATCGGGTTGGCTATTGTCGGCGCGTGGTTGAGTTCTTCGACCAGTATCTGCGC
AGTGTTGAGGCGTAGTTCCC

Downstream 100 bases:

>100_bases
CCCCGGACGTGCCACGACCGTCTGCACGATGTCGGGTATGTTGCTTCTCCGCAACAGGGTTCAGTGCATAGGGAAGGGCA
GGCCGAACGCCTGCCCTTTC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRFDILTLFPAMFQGPLTESILKRAQQAGRIEIHLHDIRQWTTDRHRTADDTPYGGGAGMVMKAEPLAAAIRAVRAADER
PGVTILLTPDGELLTQQIVRELATLPRLLLVCGHYEGIDERVRETLIDRELSIGDYVLTGGELAAMVVVDAVARLVPGVI
DSESIVEESHSDFLLEYPHYTRPAVWEGRAVPPVLLSGHHGEIARWRRAERLRRTLVRRPDLLARAAAAGVLTKADLALL
AELGWRPETSNGA

Sequences:

>Translated_253_residues
MRFDILTLFPAMFQGPLTESILKRAQQAGRIEIHLHDIRQWTTDRHRTADDTPYGGGAGMVMKAEPLAAAIRAVRAADER
PGVTILLTPDGELLTQQIVRELATLPRLLLVCGHYEGIDERVRETLIDRELSIGDYVLTGGELAAMVVVDAVARLVPGVI
DSESIVEESHSDFLLEYPHYTRPAVWEGRAVPPVLLSGHHGEIARWRRAERLRRTLVRRPDLLARAAAAGVLTKADLALL
AELGWRPETSNGA
>Mature_253_residues
MRFDILTLFPAMFQGPLTESILKRAQQAGRIEIHLHDIRQWTTDRHRTADDTPYGGGAGMVMKAEPLAAAIRAVRAADER
PGVTILLTPDGELLTQQIVRELATLPRLLLVCGHYEGIDERVRETLIDRELSIGDYVLTGGELAAMVVVDAVARLVPGVI
DSESIVEESHSDFLLEYPHYTRPAVWEGRAVPPVLLSGHHGEIARWRRAERLRRTLVRRPDLLARAAAAGVLTKADLALL
AELGWRPETSNGA

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=51.6393442622951, Blast_Score=216, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CHLAA (A9WDJ3)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001633988.1
- ProteinModelPortal:   A9WDJ3
- SMR:   A9WDJ3
- GeneID:   5825748
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0348
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27967; Mature: 27967

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDILTLFPAMFQGPLTESILKRAQQAGRIEIHLHDIRQWTTDRHRTADDTPYGGGAGM
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCE
VMKAEPLAAAIRAVRAADERPGVTILLTPDGELLTQQIVRELATLPRLLLVCGHYEGIDE
EEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RVRETLIDRELSIGDYVLTGGELAAMVVVDAVARLVPGVIDSESIVEESHSDFLLEYPHY
HHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCC
TRPAVWEGRAVPPVLLSGHHGEIARWRRAERLRRTLVRRPDLLARAAAAGVLTKADLALL
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AELGWRPETSNGA
HHHCCCCCCCCCC
>Mature Secondary Structure
MRFDILTLFPAMFQGPLTESILKRAQQAGRIEIHLHDIRQWTTDRHRTADDTPYGGGAGM
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCE
VMKAEPLAAAIRAVRAADERPGVTILLTPDGELLTQQIVRELATLPRLLLVCGHYEGIDE
EEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RVRETLIDRELSIGDYVLTGGELAAMVVVDAVARLVPGVIDSESIVEESHSDFLLEYPHY
HHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCC
TRPAVWEGRAVPPVLLSGHHGEIARWRRAERLRRTLVRRPDLLARAAAAGVLTKADLALL
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AELGWRPETSNGA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA