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The map label for this gene is ligT [C]

Identifier: 222523619

GI number: 222523619

Start: 390536

End: 391108

Strand: Reverse

Name: ligT [C]

Synonym: Chy400_0325

Alternate gene names: 222523619

Gene position: 391108-390536 (Counterclockwise)

Preceding gene: 222523620

Following gene: 222523616

Centisome position: 7.42

GC content: 64.4

Gene sequence:

>573_bases
ATGTCACAAACCCCACCTGCTGAACCAACCCTCCGCCTCTTCATCGCGCTCGAACTGCCGCAGCCAGTGCGCGATGAGCT
GAAGCGCATTCAACAACGTTTACAAGGCCACAGCCGGGCCGCACGCTGGACAGACCCAACCGGGATGCACCTGACCCTAC
ACTTTCTCGGCGAAACGCCGGAGCGCCTGGTTGAACCGTTGCTGACCGGTTTGCGGTCGGTATCGGCACCTGCCCTCTCG
CTCACCCTCGACCGCCTGGGCTGTTTTCCGGCCAACGGCGCCCTGCGCGTGGTCTGGGTTGGCCTGCGCGGCGATCTGGC
AGCGCTTGCCACCCTGCACCGGTCACTCGCTACGGTCATCCAATCGCTCGGCTTACCGACCGAAACGCGGCCTTTTGTAC
CCCACATTACCCTGGCTCGTGCGCGACCCAACGCCACAGCCGCCGATCTGCGCGCCCTGCGCACCGCATTGGACACCGTT
GCTCCGCAATCGTTGACCTGGCAGAGCGATTCGCCGCGCCTCTTCCGCAGCCAGTTGACCCCACGTGGCGCGGTGTATAC
GGTGTTGGGATAA

Upstream 100 bases:

>100_bases
TAATGCCTATCTCCTATTTTCTATTTCCTATTTTCTATCTCCTCAGTTACACTTATCATGCAAACAGGTAAAGATAATCT
GAACGCAAAGCGTATTCTGT

Downstream 100 bases:

>100_bases
CGATTCCGGACACGTCCGCTATACCGGCGGTATAGGATGCGTAGCGGCTTGTGGCCGGTGGTCGCCACATGGGAGATGGT
TGCTACCGGTCACGCTGGTG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSQTPPAEPTLRLFIALELPQPVRDELKRIQQRLQGHSRAARWTDPTGMHLTLHFLGETPERLVEPLLTGLRSVSAPALS
LTLDRLGCFPANGALRVVWVGLRGDLAALATLHRSLATVIQSLGLPTETRPFVPHITLARARPNATAADLRALRTALDTV
APQSLTWQSDSPRLFRSQLTPRGAVYTVLG

Sequences:

>Translated_190_residues
MSQTPPAEPTLRLFIALELPQPVRDELKRIQQRLQGHSRAARWTDPTGMHLTLHFLGETPERLVEPLLTGLRSVSAPALS
LTLDRLGCFPANGALRVVWVGLRGDLAALATLHRSLATVIQSLGLPTETRPFVPHITLARARPNATAADLRALRTALDTV
APQSLTWQSDSPRLFRSQLTPRGAVYTVLG
>Mature_189_residues
SQTPPAEPTLRLFIALELPQPVRDELKRIQQRLQGHSRAARWTDPTGMHLTLHFLGETPERLVEPLLTGLRSVSAPALSL
TLDRLGCFPANGALRVVWVGLRGDLAALATLHRSLATVIQSLGLPTETRPFVPHITLARARPNATAADLRALRTALDTVA
PQSLTWQSDSPRLFRSQLTPRGAVYTVLG

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20797; Mature: 20666

Theoretical pI: Translated: 11.77; Mature: 11.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTPPAEPTLRLFIALELPQPVRDELKRIQQRLQGHSRAARWTDPTGMHLTLHFLGETP
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCH
ERLVEPLLTGLRSVSAPALSLTLDRLGCFPANGALRVVWVGLRGDLAALATLHRSLATVI
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
QSLGLPTETRPFVPHITLARARPNATAADLRALRTALDTVAPQSLTWQSDSPRLFRSQLT
HHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCC
PRGAVYTVLG
CCCCEEEECC
>Mature Secondary Structure 
SQTPPAEPTLRLFIALELPQPVRDELKRIQQRLQGHSRAARWTDPTGMHLTLHFLGETP
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCH
ERLVEPLLTGLRSVSAPALSLTLDRLGCFPANGALRVVWVGLRGDLAALATLHRSLATVI
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
QSLGLPTETRPFVPHITLARARPNATAADLRALRTALDTVAPQSLTWQSDSPRLFRSQLT
HHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCC
PRGAVYTVLG
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]