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The map label for this gene is mcl1 [H]

Identifier: 222523481

GI number: 222523481

Start: 219528

End: 220574

Strand: Reverse

Name: mcl1 [H]

Synonym: Chy400_0187

Alternate gene names: 222523481

Gene position: 220574-219528 (Counterclockwise)

Preceding gene: 222523482

Following gene: 222523480

Centisome position: 4.19

GC content: 60.74

Gene sequence:

>1047_bases
ATGCGCAAGCTAGCTCACAACTTCTACAAACCGTTGGCCATCGGTGCTCCGGAGCCGATCCGCGAGCTGCCGGTTCGCCC
AGAGCGGGTCGTCCACTTTTTTCCGCCCCACGTGGAAAAGATTCGCGCCCGTATTCCCGAAGTCGCCAAACAGGTTGATG
TGCTGTGCGGCAATCTGGAAGACGCGATTCCGATGGACGCCAAAGAGGCCGCCCGCAACGGCTTTATCGAGGTAGTCAAA
GCAACCGATTTCGGCGATACCGCGCTCTGGGTGCGGGTCAATGCGCTCAACAGCCCATGGGTGCTCGACGATATTGCCGA
GATTGTGGCCGCGGTGGGCAATAAACTCGATGTGATTATGATCCCGAAGGTCGAGGGGCCGTGGGACATTCACTTCGTTG
ATCAGTATCTGGCGCTGCTCGAAGCCCGCCACCAGATCAAAAAGCCGATTCTGATCCATGCTCTGCTAGAAACCGCCCAG
GGCATGGTCAATCTGGAAGAAATTGCCGGTGCCAGCCCCCGCATGCACGGCTTCAGTCTGGGGCCGGCTGATCTCGCCGC
TTCGCGTGGCATGAAGACCACCCGTGTCGGCGGTGGGCACCCCTTCTACGGCGTGCTGGCCGACCCGCAAGAAGGTCAGG
CCGAGCGGCCATTCTATCAGCAAGACCTCTGGCACTACACGATTGCGCGGATGGTTGATGTGGCAGTTGCCCATGGCCTG
CGCGCCTTCTACGGCCCCTTCGGCGACATCAAGGATGAAGCCGCCTGCGAAGCCCAATTCCGCAACGCCTTCCTCCTCGG
CTGCACCGGTGCGTGGTCGCTCGCGCCCAACCAGATTCCCATCGCCAAGCGCGTCTTCAGCCCGGACGTGAACGAGGTGC
TCTTCGCCAAACGCATCCTGGAGGCGATGCCCGATGGTTCGGGGGTGGCGATGATTGACGGCAAGATGCAAGACGATGCG
ACCTGGAAGCAGGCGAAGGTGATCGTTGATCTGGCGCGGATGATTGCGAAGAAAGACCCCGACCTGGCCCAGGCGTATGG
TCTGTGA

Upstream 100 bases:

>100_bases
TCGCTTGCACGATGAAGGGATTGTGGCCGGGCCAGATCGGGCAGCGTAGCCGTATGCACTGCCGCCGAATGATGAGATTG
AAACACGGAGGGAGAAGATC

Downstream 100 bases:

>100_bases
CGGTGCAGCAAGGAGAGAGGGATGAGCGCTAAAACCAATCCCGGCAACTTCTTCGAGGATTTTCGGCTTGGTCAGACGAT
TGTCCACGCCACGCCGCGCA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MRKLAHNFYKPLAIGAPEPIRELPVRPERVVHFFPPHVEKIRARIPEVAKQVDVLCGNLEDAIPMDAKEAARNGFIEVVK
ATDFGDTALWVRVNALNSPWVLDDIAEIVAAVGNKLDVIMIPKVEGPWDIHFVDQYLALLEARHQIKKPILIHALLETAQ
GMVNLEEIAGASPRMHGFSLGPADLAASRGMKTTRVGGGHPFYGVLADPQEGQAERPFYQQDLWHYTIARMVDVAVAHGL
RAFYGPFGDIKDEAACEAQFRNAFLLGCTGAWSLAPNQIPIAKRVFSPDVNEVLFAKRILEAMPDGSGVAMIDGKMQDDA
TWKQAKVIVDLARMIAKKDPDLAQAYGL

Sequences:

>Translated_348_residues
MRKLAHNFYKPLAIGAPEPIRELPVRPERVVHFFPPHVEKIRARIPEVAKQVDVLCGNLEDAIPMDAKEAARNGFIEVVK
ATDFGDTALWVRVNALNSPWVLDDIAEIVAAVGNKLDVIMIPKVEGPWDIHFVDQYLALLEARHQIKKPILIHALLETAQ
GMVNLEEIAGASPRMHGFSLGPADLAASRGMKTTRVGGGHPFYGVLADPQEGQAERPFYQQDLWHYTIARMVDVAVAHGL
RAFYGPFGDIKDEAACEAQFRNAFLLGCTGAWSLAPNQIPIAKRVFSPDVNEVLFAKRILEAMPDGSGVAMIDGKMQDDA
TWKQAKVIVDLARMIAKKDPDLAQAYGL
>Mature_348_residues
MRKLAHNFYKPLAIGAPEPIRELPVRPERVVHFFPPHVEKIRARIPEVAKQVDVLCGNLEDAIPMDAKEAARNGFIEVVK
ATDFGDTALWVRVNALNSPWVLDDIAEIVAAVGNKLDVIMIPKVEGPWDIHFVDQYLALLEARHQIKKPILIHALLETAQ
GMVNLEEIAGASPRMHGFSLGPADLAASRGMKTTRVGGGHPFYGVLADPQEGQAERPFYQQDLWHYTIARMVDVAVAHGL
RAFYGPFGDIKDEAACEAQFRNAFLLGCTGAWSLAPNQIPIAKRVFSPDVNEVLFAKRILEAMPDGSGVAMIDGKMQDDA
TWKQAKVIVDLARMIAKKDPDLAQAYGL

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=325, Percent_Identity=27.0769230769231, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI87081764, Length=289, Percent_Identity=24.5674740484429, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 38368; Mature: 38368

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLAHNFYKPLAIGAPEPIRELPVRPERVVHFFPPHVEKIRARIPEVAKQVDVLCGNLE
CCHHHHHHCCCCCCCCCCHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCCH
DAIPMDAKEAARNGFIEVVKATDFGDTALWVRVNALNSPWVLDDIAEIVAAVGNKLDVIM
HCCCCCHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEE
IPKVEGPWDIHFVDQYLALLEARHQIKKPILIHALLETAQGMVNLEEIAGASPRMHGFSL
EECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEHHHHCCCCCCCCCCCC
GPADLAASRGMKTTRVGGGHPFYGVLADPQEGQAERPFYQQDLWHYTIARMVDVAVAHGL
CHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAFYGPFGDIKDEAACEAQFRNAFLLGCTGAWSLAPNQIPIAKRVFSPDVNEVLFAKRIL
HHHHCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
EAMPDGSGVAMIDGKMQDDATWKQAKVIVDLARMIAKKDPDLAQAYGL
HHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCC
>Mature Secondary Structure
MRKLAHNFYKPLAIGAPEPIRELPVRPERVVHFFPPHVEKIRARIPEVAKQVDVLCGNLE
CCHHHHHHCCCCCCCCCCHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCCH
DAIPMDAKEAARNGFIEVVKATDFGDTALWVRVNALNSPWVLDDIAEIVAAVGNKLDVIM
HCCCCCHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEE
IPKVEGPWDIHFVDQYLALLEARHQIKKPILIHALLETAQGMVNLEEIAGASPRMHGFSL
EECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEHHHHCCCCCCCCCCCC
GPADLAASRGMKTTRVGGGHPFYGVLADPQEGQAERPFYQQDLWHYTIARMVDVAVAHGL
CHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAFYGPFGDIKDEAACEAQFRNAFLLGCTGAWSLAPNQIPIAKRVFSPDVNEVLFAKRIL
HHHHCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
EAMPDGSGVAMIDGKMQDDATWKQAKVIVDLARMIAKKDPDLAQAYGL
HHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA