BacMap

Definition	Chloroflexus sp. Y-400-fl chromosome, complete genome.
Accession	NC_012032
Length	5,268,950

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The map label for this gene is echA8 [H]

Identifier: 222523406

GI number: 222523406

Start: 135384

End: 136160

Strand: Direct

Name: echA8 [H]

Synonym: Chy400_0108

Alternate gene names: 222523406

Gene position: 135384-136160 (Clockwise)

Preceding gene: 222523404

Following gene: 222523407

Centisome position: 2.57

GC content: 58.17

Gene sequence:

>777_bases
ATGAGTGAAGAGTCTCTGGTTCTCAGCACAATTGAAGGCCCCATCGCCATCCTCACCCTCAATCGCCCCCAGGCCCTCAA
TGCGCTCAGTCCGGCCTTGATTGATGACCTCATTCGCCATTTAGAAGCCTGCGATGCCGATGACACAATCCGCGTGATCA
TTATCACCGGCGCCGGACGGGCATTTGCTGCCGGCGCTGACATCAAAGCGATGGCCAATGCCACGCCTATTGATATGCTC
ACCAGTGGCATGATTGCGCGCTGGGCACGCATCGCCGCGGTGCGCAAACCGGTGATTGCTGCCGTGAATGGGTATGCGCT
CGGTGGTGGTTGTGAATTGGCAATGATGTGCGACATCATCATCGCCAGTGAAAACGCGCAGTTCGGACAACCGGAAATCA
ATCTGGGCATCATTCCCGGTGCTGGTGGCACCCAACGGCTGACCCGCGCCCTTGGCCCGTATCGCGCAATGGAATTGATC
CTGACCGGCGCGACCATCAGTGCTCAGGAAGCTCTCGCCCACGGCCTGGTGTGCCGGGTCTGCCCGCCTGAAAGCCTGCT
CGATGAAGCCCGTCGGATCGCGCAAACCATTGCCACCAAATCACCACTGGCTGTACAGTTGGCGAAAGAGGCAGTCCGTA
TGGCCGCCGAAACCACTGTGCGCGAGGGGTTGGCTATCGAGCTGCGTAACTTCTATCTGCTGTTTGCCAGTGCTGACCAA
AAAGAGGGGATGCAGGCATTTATCGAGAAACGCGCTCCCAACTTCAGTGGTCGTTGA

Upstream 100 bases:

>100_bases
CTTTCGCAAAAGCTATCCAATCCTGCACACGTGCATCTGTTACAGGGTATTATTGTCGGCAAACGACAGTCCTGTCGTTT
ATGTACAAGGAGATCAACGT

Downstream 100 bases:

>100_bases
TCACGCGCAGAACATGGCAGCAGGGGCAATACCTGCACGTACTGCCTCCTGCCGCCATACTACCAGATGATCGAGCAGTA
AAGGGTAAATACTCTATCAA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSEESLVLSTIEGPIAILTLNRPQALNALSPALIDDLIRHLEACDADDTIRVIIITGAGRAFAAGADIKAMANATPIDML
TSGMIARWARIAAVRKPVIAAVNGYALGGGCELAMMCDIIIASENAQFGQPEINLGIIPGAGGTQRLTRALGPYRAMELI
LTGATISAQEALAHGLVCRVCPPESLLDEARRIAQTIATKSPLAVQLAKEAVRMAAETTVREGLAIELRNFYLLFASADQ
KEGMQAFIEKRAPNFSGR

Sequences:

>Translated_258_residues
MSEESLVLSTIEGPIAILTLNRPQALNALSPALIDDLIRHLEACDADDTIRVIIITGAGRAFAAGADIKAMANATPIDML
TSGMIARWARIAAVRKPVIAAVNGYALGGGCELAMMCDIIIASENAQFGQPEINLGIIPGAGGTQRLTRALGPYRAMELI
LTGATISAQEALAHGLVCRVCPPESLLDEARRIAQTIATKSPLAVQLAKEAVRMAAETTVREGLAIELRNFYLLFASADQ
KEGMQAFIEKRAPNFSGR
>Mature_257_residues
SEESLVLSTIEGPIAILTLNRPQALNALSPALIDDLIRHLEACDADDTIRVIIITGAGRAFAAGADIKAMANATPIDMLT
SGMIARWARIAAVRKPVIAAVNGYALGGGCELAMMCDIIIASENAQFGQPEINLGIIPGAGGTQRLTRALGPYRAMELIL
TGATISAQEALAHGLVCRVCPPESLLDEARRIAQTIATKSPLAVQLAKEAVRMAAETTVREGLAIELRNFYLLFASADQK
EGMQAFIEKRAPNFSGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=54.0983606557377, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=34.6613545816733, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=38.4, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=31.3207547169811, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=39.7849462365591, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI150378541, Length=249, Percent_Identity=32.9317269076305, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=29.2792792792793, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=29.2792792792793, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=29.2792792792793, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=34.6153846153846, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI221136756, Length=256, Percent_Identity=25.390625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221136753, Length=256, Percent_Identity=25.390625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221307494, Length=256, Percent_Identity=25.390625, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI25453481, Length=258, Percent_Identity=24.031007751938, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI51538573, Length=258, Percent_Identity=24.031007751938, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=27.9475982532751, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=27.9475982532751, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=204, Percent_Identity=30.3921568627451, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594471, Length=204, Percent_Identity=30.3921568627451, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI116235487, Length=257, Percent_Identity=25.2918287937743, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI4757968, Length=256, Percent_Identity=23.828125, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI49355787, Length=256, Percent_Identity=23.828125, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI4757966, Length=247, Percent_Identity=24.2914979757085, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI51538581, Length=247, Percent_Identity=24.2914979757085, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI62530384, Length=242, Percent_Identity=26.4462809917355, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI157694520, Length=181, Percent_Identity=28.1767955801105, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=44.2105263157895, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=50.4098360655738, Blast_Score=240, Evalue=6e-65,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=40.9961685823755, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=37.2623574144487, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=36, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=33.9622641509434, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1790281, Length=198, Percent_Identity=32.3232323232323, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788682, Length=200, Percent_Identity=35, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=53.2786885245902, Blast_Score=267, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=48.3471074380165, Blast_Score=231, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17540714, Length=216, Percent_Identity=34.7222222222222, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=255, Percent_Identity=30.5882352941176, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=29.9212598425197, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=179, Percent_Identity=35.7541899441341, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=31.9502074688797, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=179, Percent_Identity=35.7541899441341, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=209, Percent_Identity=30.1435406698565, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=33.5195530726257, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=254, Percent_Identity=29.9212598425197, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=179, Percent_Identity=35.7541899441341, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=28.1746031746032, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=195, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=195, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=198, Percent_Identity=28.2828282828283, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=203, Percent_Identity=28.0788177339901, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=54.5454545454545, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=54.5454545454545, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=36.6141732283465, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=28.4090909090909, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19920382, Length=239, Percent_Identity=32.2175732217573, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357171, Length=198, Percent_Identity=32.8282828282828, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=31.6, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571730, Length=188, Percent_Identity=30.8510638297872, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28571729, Length=188, Percent_Identity=30.8510638297872, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24654903, Length=173, Percent_Identity=28.9017341040462, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24583165, Length=239, Percent_Identity=26.7782426778243, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921018, Length=244, Percent_Identity=29.0983606557377, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583077, Length=176, Percent_Identity=30.6818181818182, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583079, Length=176, Percent_Identity=30.6818181818182, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19921000, Length=176, Percent_Identity=30.6818181818182, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45550169, Length=253, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27336; Mature: 27205

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEESLVLSTIEGPIAILTLNRPQALNALSPALIDDLIRHLEACDADDTIRVIIITGAGR
CCCCCEEEEECCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFAAGADIKAMANATPIDMLTSGMIARWARIAAVRKPVIAAVNGYALGGGCELAMMCDII
EEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEECCEEECCCCHHEEEEEEE
IASENAQFGQPEINLGIIPGAGGTQRLTRALGPYRAMELILTGATISAQEALAHGLVCRV
EECCCCCCCCCCEEEEEEECCCCHHHHHHHHCHHHHHHHHEECCCCCHHHHHHHCCEEEE
CPPESLLDEARRIAQTIATKSPLAVQLAKEAVRMAAETTVREGLAIELRNFYLLFASADQ
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCH
KEGMQAFIEKRAPNFSGR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEESLVLSTIEGPIAILTLNRPQALNALSPALIDDLIRHLEACDADDTIRVIIITGAGR
CCCCEEEEECCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFAAGADIKAMANATPIDMLTSGMIARWARIAAVRKPVIAAVNGYALGGGCELAMMCDII
EEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEECCEEECCCCHHEEEEEEE
IASENAQFGQPEINLGIIPGAGGTQRLTRALGPYRAMELILTGATISAQEALAHGLVCRV
EECCCCCCCCCCEEEEEEECCCCHHHHHHHHCHHHHHHHHEECCCCCHHHHHHHCCEEEE
CPPESLLDEARRIAQTIATKSPLAVQLAKEAVRMAAETTVREGLAIELRNFYLLFASADQ
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCH
KEGMQAFIEKRAPNFSGR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]