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The map label for this gene is brnQ [H]

Identifier: 222095174

GI number: 222095174

Start: 1485884

End: 1487239

Strand: Reverse

Name: brnQ [H]

Synonym: BCQ_1514

Alternate gene names: 222095174

Gene position: 1487239-1485884 (Counterclockwise)

Preceding gene: 222095175

Following gene: 222095164

Centisome position: 28.52

GC content: 37.32

Gene sequence:

>1356_bases
ATGAACACTGTATCAAAAAAACATATTTTTTTCACGGGTCTTATGCTATTTTCTTTATTTTTTGGGGCAGGTAATTTAAT
TTTCCCTCCCATGCTTGGACAAAATGCAGGTGAAAACTTTTGGCCGGCAATGATTGGATTTTTACTAACAGGAGTCGGTT
TACCACTACTAACAGTTATCGCAATTTCTTTATCAGGAAATGGTATGCAACAACTTGCAAGTCATGTTCACCCATTATTC
GGAATATTCTTTACGGTAGTTGTATACATCGCCATTGGCCCTTCTATGGGTATTCCACGCGTTGCAAATGTCGCTTATGA
AATGGGCGCTAGTTCTTTTTTACCAGAAACGATTCGCTCTAGCAACCTCGTACTTTTTGTATATACAATCATCTTCTTTG
CTATCGTATATTGGCTGAGTTTGAATCCATCTAAACTTGTAGATCGTATTGGAAATATATTAACACCTATTTTATTACTC
TCTATTTTCTTATTGTTTGTTAAAAGTGTCTTTACACCACTTGGACAATCAGGACCTGCAATGCAAGAGTATCAAACTTC
TCCGATTTTCAAAGGCTTTATGGAAGGATACTTAACGATGGATACCATTTCAGCACTTGCATTTGGAATTATCGTTGTAA
ATACCATTCGCTCTAAAGGTGTAAAGGATCGTAAATCCATTGCCATCGCAACAGCCAAAGCTGGACTTATTGCTGCTACT
GGCCTCGTACTTGTATATGGTGCGCTTGGCTGGCTCGGTGCAACTAGCGTCTCGCTCGGATATGCAAAGAATGGTGGACA
ATTACTTACTGTGATCGTACAACAATTATTCGGTCCATATGGTCTACTTTTATTATCTCTTATCGTTACACTCGCTTGCT
TAACAACATGTGTTGGACTTGTATCTGCATGTAGTCAATATTTCTCGACATTATTGACTAAATTTTCATACAAAACGTTC
GCAAGTATCATTTGTGTATTGGGATTATTAGTTGCCAACTTAGGTTTAACAAAAATCATTTCCATCTCTGTTCCAATTCT
ACTTGTTGTATATCCAGTTGCGATTGTACTTGTTCTACTATCGTTACTTCATAAATATTTTGGTGGATATCGCTCTGTTT
ATGTAGGTGCTTTAATTGGAGCTGCAATTGTAAGCGTATTTGATGGATTAAAACAAGGTGGTATTTCAGTTTCATTTATC
ACATCATATTTTGAATTTATCCCTTTATATAATGAAGGAATAGGTTGGCTAGTACCTGCATTAGCCGGAGCTCTTATTGG
CCTTGCAATCGCTAAGCTAAGTGGCGCGAAAAAAGTTCCCTTACTAACCGAATCTCGTGAAGTAAAAGCATCCTAA

Upstream 100 bases:

>100_bases
TAAATTAGGGTGGTATCGCGGGTAAATATAACTCGTCCCTTTCTTTAGGGACGAGTTTTTTGTGTTCTTACAAATGAATT
TTAAAAAAGGAGGAAAAACA

Downstream 100 bases:

>100_bases
AAAAACTCCCAAAAGGGAGTTTTTTACTTTCGATAAGCAGTATGATTAAAACTGCTTATTTTATACGCTTTCGTATAATA
GTCAATATTTGTATCCACTG

Product: branched-chain amino acid transporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGNGMQQLASHVHPLF
GIFFTVVVYIAIGPSMGIPRVANVAYEMGASSFLPETIRSSNLVLFVYTIIFFAIVYWLSLNPSKLVDRIGNILTPILLL
SIFLLFVKSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNTIRSKGVKDRKSIAIATAKAGLIAAT
GLVLVYGALGWLGATSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLSLIVTLACLTTCVGLVSACSQYFSTLLTKFSYKTF
ASIICVLGLLVANLGLTKIISISVPILLVVYPVAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFI
TSYFEFIPLYNEGIGWLVPALAGALIGLAIAKLSGAKKVPLLTESREVKAS

Sequences:

>Translated_451_residues
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGNGMQQLASHVHPLF
GIFFTVVVYIAIGPSMGIPRVANVAYEMGASSFLPETIRSSNLVLFVYTIIFFAIVYWLSLNPSKLVDRIGNILTPILLL
SIFLLFVKSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNTIRSKGVKDRKSIAIATAKAGLIAAT
GLVLVYGALGWLGATSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLSLIVTLACLTTCVGLVSACSQYFSTLLTKFSYKTF
ASIICVLGLLVANLGLTKIISISVPILLVVYPVAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFI
TSYFEFIPLYNEGIGWLVPALAGALIGLAIAKLSGAKKVPLLTESREVKAS
>Mature_451_residues
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGNGMQQLASHVHPLF
GIFFTVVVYIAIGPSMGIPRVANVAYEMGASSFLPETIRSSNLVLFVYTIIFFAIVYWLSLNPSKLVDRIGNILTPILLL
SIFLLFVKSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNTIRSKGVKDRKSIAIATAKAGLIAAT
GLVLVYGALGWLGATSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLSLIVTLACLTTCVGLVSACSQYFSTLLTKFSYKTF
ASIICVLGLLVANLGLTKIISISVPILLVVYPVAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFI
TSYFEFIPLYNEGIGWLVPALAGALIGLAIAKLSGAKKVPLLTESREVKAS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=437, Percent_Identity=37.5286041189931, Blast_Score=298, Evalue=7e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48031; Mature: 48031

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVI
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AISLSGNGMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYEMGASSFLPETIRS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHCCHHHHCC
SNLVLFVYTIIFFAIVYWLSLNPSKLVDRIGNILTPILLLSIFLLFVKSVFTPLGQSGPA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
MQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNTIRSKGVKDRKSIAIATAKAGLIAAT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GLVLVYGALGWLGATSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLSLIVTLACLTTCVGL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSACSQYFSTLLTKFSYKTFASIICVLGLLVANLGLTKIISISVPILLVVYPVAIVLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFITSYFEFIPLYNEGIGWLVPA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LAGALIGLAIAKLSGAKKVPLLTESREVKAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVI
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AISLSGNGMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYEMGASSFLPETIRS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHCCHHHHCC
SNLVLFVYTIIFFAIVYWLSLNPSKLVDRIGNILTPILLLSIFLLFVKSVFTPLGQSGPA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
MQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNTIRSKGVKDRKSIAIATAKAGLIAAT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GLVLVYGALGWLGATSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLSLIVTLACLTTCVGL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSACSQYFSTLLTKFSYKTFASIICVLGLLVANLGLTKIISISVPILLVVYPVAIVLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFITSYFEFIPLYNEGIGWLVPA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LAGALIGLAIAKLSGAKKVPLLTESREVKAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]