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The map label for this gene is gloA [H]

Identifier: 222094358

GI number: 222094358

Start: 668783

End: 669169

Strand: Direct

Name: gloA [H]

Synonym: BCQ_0672

Alternate gene names: 222094358

Gene position: 668783-669169 (Clockwise)

Preceding gene: 222094354

Following gene: 222094359

Centisome position: 12.83

GC content: 35.66

Gene sequence:

>387_bases
ATGAATATATGTAGAGTGCACCACGTTGCAATTATTTGTTCGAATTATGAAGTGTCAAAGGATTTTTATAATCGAATATT
AGGTTTTAAAGCGATAAATGAAGTATATAGGAAGGAACGAGATTCTTATAAGTTAGATTTATGTGTAGGAGAAGAGTATC
AAATTGAATTATTTTCATTTCCAAGTCCGCCTGAGCGCCCGAGTTTCCCAGAAGCGGCTGGACTTAGACATTTAGCATTT
GCTGTTACAAATATAGAAGAGGCTGTGAAGCATTTACGCCAATGTGGTGTTAAGACGGAATCAATCCGTATTGATGAAAT
AACGGAGAAGAAATTTGTCTTTTTCCAAGACCCTGATGGTTTACCTTTAGAATTGTATGAGGTTTGA

Upstream 100 bases:

>100_bases
ATAAATTTCATAGTAAGCCCCCTAAAAGAGATATTCTGCTATATATGTTTTTCATTTCAAGGGAGAATATGCATCTTTGG
TAAAGAAAGGAATAGAGAGC

Downstream 100 bases:

>100_bases
AATTGGTGAATTTTATATAGGGTTGCTTTTGAGTAAATGAAGAAACTAAAGTGAAACTTCTTTTTTAAAGGAGGTGTCAC
TTTAGCTAAGAAGGCTAAAG

Product: lactoylglutathione lyase (glyoxylase i)

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MNICRVHHVAIICSNYEVSKDFYNRILGFKAINEVYRKERDSYKLDLCVGEEYQIELFSFPSPPERPSFPEAAGLRHLAF
AVTNIEEAVKHLRQCGVKTESIRIDEITEKKFVFFQDPDGLPLELYEV

Sequences:

>Translated_128_residues
MNICRVHHVAIICSNYEVSKDFYNRILGFKAINEVYRKERDSYKLDLCVGEEYQIELFSFPSPPERPSFPEAAGLRHLAF
AVTNIEEAVKHLRQCGVKTESIRIDEITEKKFVFFQDPDGLPLELYEV
>Mature_128_residues
MNICRVHHVAIICSNYEVSKDFYNRILGFKAINEVYRKERDSYKLDLCVGEEYQIELFSFPSPPERPSFPEAAGLRHLAF
AVTNIEEAVKHLRQCGVKTESIRIDEITEKKFVFFQDPDGLPLELYEV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ywkD [H]

Homologues:

Organism=Escherichia coli, GI87081697, Length=127, Percent_Identity=61.4173228346457, Blast_Score=162, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14901; Mature: 14901

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNICRVHHVAIICSNYEVSKDFYNRILGFKAINEVYRKERDSYKLDLCVGEEYQIELFSF
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC
PSPPERPSFPEAAGLRHLAFAVTNIEEAVKHLRQCGVKTESIRIDEITEKKFVFFQDPDG
CCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEECCCEEEEEECCCC
LPLELYEV
CCEEEEEC
>Mature Secondary Structure
MNICRVHHVAIICSNYEVSKDFYNRILGFKAINEVYRKERDSYKLDLCVGEEYQIELFSF
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC
PSPPERPSFPEAAGLRHLAFAVTNIEEAVKHLRQCGVKTESIRIDEITEKKFVFFQDPDG
CCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEECCCEEEEEECCCC
LPLELYEV
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9723924; 9278503 [H]