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The map label for this gene is bfrA [H]

Identifier: 220933076

GI number: 220933076

Start: 2445697

End: 2446329

Strand: Reverse

Name: bfrA [H]

Synonym: Hore_22440

Alternate gene names: 220933076

Gene position: 2446329-2445697 (Counterclockwise)

Preceding gene: 220933079

Following gene: 220933075

Centisome position: 94.89

GC content: 44.23

Gene sequence:

>633_bases
ATGTACTATTTAAGAAGCTTTAAAGACTCGGTGGGAGATGTAGATTTGATAGTACACAATGATAGACTCCACCTTTTCCA
TCTGGTATCACCGGGGAACAGTGCGGTAAGGCACCTTGTTTCTGATGATGGGATAATCTGGGATAGCCTCCCTACGGCCA
TTACTATCGGTGATACAGGGAGCTATGATGATGATCGTATCTGGACCATGGGTGTTACCAGACATAAGGGGAAGTTTTAT
ATGTTCTATACTGCCTGTTCCACCAGGGAGGCTGGTCGGGTTCAGAGGACAGCCATGGCTGTGTCTCCTGACCTGATAAA
CTGGGAAAAATACGATGGGAATCCTGTTTTATCCGCCGACAGCCGTTGGTATGAAAATGAACTGGGAGACAAAATAATGG
TCTCCTGGCGGGACCCCAAGATATATTACGAAAACGGTAAGTACTATATGGTTATTTCCAGCCGGAGTAATTCAGGTCCT
TTCCTCAGGAGAGGTGTGGTAGCCCTGGCTGTTTCTGATAATTTAATTGACTGGGAGGTAAAAAAACCACTATTTGCCCC
GGGGCAGTTTTATGACCTGGAATGTCCCCAGTTATTTAAAATAGTGATTATTATTACATATTTGCCTCAATAA

Upstream 100 bases:

>100_bases
TATAATATGATAGGTCTGGACGTTACAATGAATACTATAGTTAAATCAGTTAGTGAGTTTATGTGGTATATTGTAATATG
AAAACAGGAGTGGATGTTAA

Downstream 100 bases:

>100_bases
TTGAAGACAGGAGTCAGCGTTACTGGGTGTCGGAAAGACTGGAAGGCCCCTACCGGGCATTAGGTGATAACCGGCTTTTT
GTTCCTCATAGCCATTATGC

Product: glycosyl hydrolase family 32 domain-containing protein

Products: NA

Alternate protein names: Invertase; Sucrase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MYYLRSFKDSVGDVDLIVHNDRLHLFHLVSPGNSAVRHLVSDDGIIWDSLPTAITIGDTGSYDDDRIWTMGVTRHKGKFY
MFYTACSTREAGRVQRTAMAVSPDLINWEKYDGNPVLSADSRWYENELGDKIMVSWRDPKIYYENGKYYMVISSRSNSGP
FLRRGVVALAVSDNLIDWEVKKPLFAPGQFYDLECPQLFKIVIIITYLPQ

Sequences:

>Translated_210_residues
MYYLRSFKDSVGDVDLIVHNDRLHLFHLVSPGNSAVRHLVSDDGIIWDSLPTAITIGDTGSYDDDRIWTMGVTRHKGKFY
MFYTACSTREAGRVQRTAMAVSPDLINWEKYDGNPVLSADSRWYENELGDKIMVSWRDPKIYYENGKYYMVISSRSNSGP
FLRRGVVALAVSDNLIDWEVKKPLFAPGQFYDLECPQLFKIVIIITYLPQ
>Mature_210_residues
MYYLRSFKDSVGDVDLIVHNDRLHLFHLVSPGNSAVRHLVSDDGIIWDSLPTAITIGDTGSYDDDRIWTMGVTRHKGKFY
MFYTACSTREAGRVQRTAMAVSPDLINWEKYDGNPVLSADSRWYENELGDKIMVSWRDPKIYYENGKYYMVISSRSNSGP
FLRRGVVALAVSDNLIDWEVKKPLFAPGQFYDLECPQLFKIVIIITYLPQ

Specific function: Hydrolysis of sucrose, raffinose, inulin and levan. Specific for the fructose moiety and the beta-anomeric configuration of the glycosidic linkages of its substrates. The enzyme released fructose from sucrose and raffinose, and the fructose polymer inulin

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 24119; Mature: 24119

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYLRSFKDSVGDVDLIVHNDRLHLFHLVSPGNSAVRHLVSDDGIIWDSLPTAITIGDTG
CCCHHCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHCCCCEEEECCCCEEEECCCC
SYDDDRIWTMGVTRHKGKFYMFYTACSTREAGRVQRTAMAVSPDLINWEKYDGNPVLSAD
CCCCCEEEEEEEEEECCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCEEECCCEEEECC
SRWYENELGDKIMVSWRDPKIYYENGKYYMVISSRSNSGPFLRRGVVALAVSDNLIDWEV
CCHHHHHCCCEEEEEEECCEEEEECCEEEEEEECCCCCCCCHHCCEEEEEEECCEEEEEE
KKPLFAPGQFYDLECPQLFKIVIIITYLPQ
CCCEECCCCEEECCCHHHEEEEEEEEECCC
>Mature Secondary Structure
MYYLRSFKDSVGDVDLIVHNDRLHLFHLVSPGNSAVRHLVSDDGIIWDSLPTAITIGDTG
CCCHHCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHCCCCEEEECCCCEEEECCCC
SYDDDRIWTMGVTRHKGKFYMFYTACSTREAGRVQRTAMAVSPDLINWEKYDGNPVLSAD
CCCCCEEEEEEEEEECCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCEEECCCEEEECC
SRWYENELGDKIMVSWRDPKIYYENGKYYMVISSRSNSGPFLRRGVVALAVSDNLIDWEV
CCHHHHHCCCEEEEEEECCEEEEECCEEEEEEECCCCCCCCHHCCEEEEEEECCEEEEEE
KKPLFAPGQFYDLECPQLFKIVIIITYLPQ
CCCEECCCCEEECCCHHHEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9720201; 10360571 [H]