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The map label for this gene is thiI

Identifier: 220933038

GI number: 220933038

Start: 2402428

End: 2403618

Strand: Direct

Name: thiI

Synonym: Hore_22060

Alternate gene names: 220933038

Gene position: 2402428-2403618 (Clockwise)

Preceding gene: 220933037

Following gene: 220933039

Centisome position: 93.18

GC content: 41.73

Gene sequence:

>1191_bases
ATGTACAAAGTTATTCTGATTCGCTATGGAGAGATAGCCCTTAAGGGTCAAAACAGGCATGTTTTTATCAATAAACTGAT
TGAAAATATAAAAGAAAGCTTAAAAGGAGTCGGTGAATACAAACTTAAAAAAACCCTAGGCCGAATCTATGTTTTTCCTA
ACGATAATATAAATCAGTTTATAGAAAAACTTAAAATGGTACCCGGTATAGTATCCCTGAGTCCCACAGCTGTCTGTCCG
CTTGATTTTGACAGTCTTAAAGAAACATCAGTAAAGGTCTTAAAAGATGCTATTAATGATTACCCGGCCACTTTTAAAGT
AGAAACCAGACGGGCCAACAAACAATTCCCCTATAAAAGCCCGGAGGTCAGCCGGGAGATCGGAGCTCATCTTTTAAGAA
GCATAAATACCCCTGACCACAATATCCTGACAGTTGATGTCCATAACCCCCGGTACACTTTAAAAATAGAAATTAGAAAG
AAAAATATATATGTCTTCACAAGATCTATTTCAGGGCCGGGTGGGTTGCCGGTCGGTTCCAGCGGTAAGGGGCTTTTACT
CCTGTCGGGGGGAATTGATAGCCCGGTAGCCGGCTGGCTGGCCATGAAAAGGGGAATTGAGCTTGAAGCTCTATATTTCC
ATAGCTTCCCCTATACCAGTGACCGGGCTAAAGAAAAGGTTATTGACTTAACTAAAGTATTAAGCCGCTACTGTAAAAAA
ATAAAATTATATGTGGGTTATTTTACAGATATTCAGAGGGCTATCCAGGAAAACTGCCCTCAGAAATACTATATCACCAT
CATGAGGAGAATGATGTACAGGATGGCTGAGAGAATCGCCAGGAAAAACGGAGATCTGGTTTTAATTACCGGAGAAAGTG
TCGGCCAGGTGGCCAGTCAGACCCTGGAAAGTATGAATGTCATAAATGAAGTAACCAATATGCCTGTCCTCCGTCCCCTG
GCAACAATGAATAAAACTGAAATTATGGACCTGGCCCGGGAGATCGGAACCTATGATATCTCAATTCTTCCCCATGAAGA
CTGCTGTACCATCTTTGTCCCCAGACATCCGGTTACCAGGCCCAGGCTTGACAGAACCCTCAAGGCTGAAGAAAAATTAA
AAGCCGAAATAGACGGTCTCCTTGAAGATGCCATAGAAAAAACCGAAATCCTTGAAATAAAACATGGTTAA

Upstream 100 bases:

>100_bases
TCTGTCTGAATATAACACAGAAACAGACTTAAACACTGCAGTAAAAACCCTGGCAGAACAGTTGCGGTATTTCTTTTAAC
ACTATAGGGAGGTGAACCAT

Downstream 100 bases:

>100_bases
TATGACTTGAAAATTAAAACATGGTTACTATAAAATTACCCCGGGGAATCTCCCCGGGGTAACGTATATGAAGAGGTATG
ATGTTATGGATGAGGCCTCA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MYKVILIRYGEIALKGQNRHVFINKLIENIKESLKGVGEYKLKKTLGRIYVFPNDNINQFIEKLKMVPGIVSLSPTAVCP
LDFDSLKETSVKVLKDAINDYPATFKVETRRANKQFPYKSPEVSREIGAHLLRSINTPDHNILTVDVHNPRYTLKIEIRK
KNIYVFTRSISGPGGLPVGSSGKGLLLLSGGIDSPVAGWLAMKRGIELEALYFHSFPYTSDRAKEKVIDLTKVLSRYCKK
IKLYVGYFTDIQRAIQENCPQKYYITIMRRMMYRMAERIARKNGDLVLITGESVGQVASQTLESMNVINEVTNMPVLRPL
ATMNKTEIMDLAREIGTYDISILPHEDCCTIFVPRHPVTRPRLDRTLKAEEKLKAEIDGLLEDAIEKTEILEIKHG

Sequences:

>Translated_396_residues
MYKVILIRYGEIALKGQNRHVFINKLIENIKESLKGVGEYKLKKTLGRIYVFPNDNINQFIEKLKMVPGIVSLSPTAVCP
LDFDSLKETSVKVLKDAINDYPATFKVETRRANKQFPYKSPEVSREIGAHLLRSINTPDHNILTVDVHNPRYTLKIEIRK
KNIYVFTRSISGPGGLPVGSSGKGLLLLSGGIDSPVAGWLAMKRGIELEALYFHSFPYTSDRAKEKVIDLTKVLSRYCKK
IKLYVGYFTDIQRAIQENCPQKYYITIMRRMMYRMAERIARKNGDLVLITGESVGQVASQTLESMNVINEVTNMPVLRPL
ATMNKTEIMDLAREIGTYDISILPHEDCCTIFVPRHPVTRPRLDRTLKAEEKLKAEIDGLLEDAIEKTEILEIKHG
>Mature_396_residues
MYKVILIRYGEIALKGQNRHVFINKLIENIKESLKGVGEYKLKKTLGRIYVFPNDNINQFIEKLKMVPGIVSLSPTAVCP
LDFDSLKETSVKVLKDAINDYPATFKVETRRANKQFPYKSPEVSREIGAHLLRSINTPDHNILTVDVHNPRYTLKIEIRK
KNIYVFTRSISGPGGLPVGSSGKGLLLLSGGIDSPVAGWLAMKRGIELEALYFHSFPYTSDRAKEKVIDLTKVLSRYCKK
IKLYVGYFTDIQRAIQENCPQKYYITIMRRMMYRMAERIARKNGDLVLITGESVGQVASQTLESMNVINEVTNMPVLRPL
ATMNKTEIMDLAREIGTYDISILPHEDCCTIFVPRHPVTRPRLDRTLKAEEKLKAEIDGLLEDAIEKTEILEIKHG

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=389, Percent_Identity=28.2776349614396, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_HALOH (B8D0L5)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002509946.1
- ProteinModelPortal:   B8D0L5
- SMR:   B8D0L5
- GeneID:   7313754
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_22060
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45016; Mature: 45016

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS51165 THUMP

Important sites: BINDING 269-269 BINDING 291-291 BINDING 300-300

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVILIRYGEIALKGQNRHVFINKLIENIKESLKGVGEYKLKKTLGRIYVFPNDNINQF
CEEEEEEEECCEEEECCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCCCHHHH
IEKLKMVPGIVSLSPTAVCPLDFDSLKETSVKVLKDAINDYPATFKVETRRANKQFPYKS
HHHHHHCCCCEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
PEVSREIGAHLLRSINTPDHNILTVDVHNPRYTLKIEIRKKNIYVFTRSISGPGGLPVGS
CHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEEEECEEEEEEEECCCCCCCCCCC
SGKGLLLLSGGIDSPVAGWLAMKRGIELEALYFHSFPYTSDRAKEKVIDLTKVLSRYCKK
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
IKLYVGYFTDIQRAIQENCPQKYYITIMRRMMYRMAERIARKNGDLVLITGESVGQVASQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
TLESMNVINEVTNMPVLRPLATMNKTEIMDLAREIGTYDISILPHEDCCTIFVPRHPVTR
HHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCC
PRLDRTLKAEEKLKAEIDGLLEDAIEKTEILEIKHG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure
MYKVILIRYGEIALKGQNRHVFINKLIENIKESLKGVGEYKLKKTLGRIYVFPNDNINQF
CEEEEEEEECCEEEECCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCCCHHHH
IEKLKMVPGIVSLSPTAVCPLDFDSLKETSVKVLKDAINDYPATFKVETRRANKQFPYKS
HHHHHHCCCCEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
PEVSREIGAHLLRSINTPDHNILTVDVHNPRYTLKIEIRKKNIYVFTRSISGPGGLPVGS
CHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEEEECEEEEEEEECCCCCCCCCCC
SGKGLLLLSGGIDSPVAGWLAMKRGIELEALYFHSFPYTSDRAKEKVIDLTKVLSRYCKK
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
IKLYVGYFTDIQRAIQENCPQKYYITIMRRMMYRMAERIARKNGDLVLITGESVGQVASQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
TLESMNVINEVTNMPVLRPLATMNKTEIMDLAREIGTYDISILPHEDCCTIFVPRHPVTR
HHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCC
PRLDRTLKAEEKLKAEIDGLLEDAIEKTEILEIKHG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA