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The map label for this gene is pleD [H]

Identifier: 220932964

GI number: 220932964

Start: 2321091

End: 2322623

Strand: Direct

Name: pleD [H]

Synonym: Hore_21320

Alternate gene names: 220932964

Gene position: 2321091-2322623 (Clockwise)

Preceding gene: 220932963

Following gene: 220932965

Centisome position: 90.03

GC content: 40.51

Gene sequence:

>1533_bases
ATGTCCTATTGCCTTATTTTTCTCACAGTTCTCATCGCCATTACCACAGGTTATTACCGGTTCTTTTGCAAATTAAAAAC
CAACAAAATTATTTTACTGGCAGGATGTCTAATGGTAATTGAAATCAACTATATCATCCTCAAAAGCCCCCTGACCAACT
GGCTTTTTTTTGTTTTTATTTATCTGGCCTCAATTTCCTTTTTTGTTAAAGGGGGGCTTATAGCAGCTCTGATAAGTATA
ATATTTACATATCTTCAATCCCCACCTGAGACCGGTCTTAATATCTATCTTTTCGCTGGCTATATTATCATGGGTGCTGT
GACCGGCAAATACTCAGATTATATCCATACCAGCATATTAAAAAAGGCGTCCTGGCAAAAGCAGCTATATCAGCAGGCCA
AGGAATTAAATATACTTCGGGAAATAAGCACAGCCCTCCAGAGTACATTAAAGCTGGACAAACTCCTTCATATTATTCTT
ACCGCTATTACAGCCGGTTATGGACTCGGTTTTAACAGGGCCATTCTTCTTCTGGTATCCCGGGACGAAAAATATCTTGA
AGGGAAATTTGGGATAGGACCTATGAATAAGGTCGAGGGTTATGAGATATGGGAAAATGTAGTAGCTGCCAAAATGAACA
TGAAAGACTTTATCGGCTTAAAGGAAAAGGCACGATTGAAGGATAAAACATTAAATGATCTGGTCCAGTCATTAAAAATT
CCCCTGAATCCTTCCAGCCAGGGGGGTGTTCTGGCCGAAGCAATTAAATATCAAAAACCAATCATCATTAAAAGTATTGA
TGGTAATGATCCCGTCCAGGTCAGGCTGGCCAGTTATTTTGGCATGAGTGAATTTGCTGTCATCCCCCTGATAAACCAGG
GGAAACCCGTTGGTGCCATTATTATAGATAATATTGTCAACCATAAACCGATAACGGTAAAAGACCTGGATAGTATTACC
CCTCTTGCCATCCAGGCCGCCATGGCTATAGACAATGCCAATCTCTATAACAAGACCCAGAAGATGGCCATCACCGATGG
TTTAACAGGCCTTTATAACCAGCGTTATTTCCTCGAGGTAATTGACAAATACTTTAAAACAGCCCGGGAGAAAAACCGTC
ACCTATCTATATTAATAATGGATATAGATAATTTTAAAAATTATAATGATGTTAATGGCCATCTTGCCGGTAATACAGCC
CTTAGTCGATTAACTGATATTATAAAGAAAAGTATCAGGAAAAAAGATGTCCCCTGCCGGTTTGGCGGTGAAGAGTTTGC
TGTGTTATTCCCCGATACAACCAGGGACGAGGCCTACAACCTGGCCGAAACTATTAGAAAAAATATAGAAAAAACCCCCT
TTTATAATGAAGATGTCCAGCCAGGGGGGCGGTTGACTGTAAGCCTGGGACTGGCTATCTTTCCTGATGATAGCCAGGAG
GTTAAAGACCTGCTGGATAAAGCTGACCAGGCCCTTTATAAGGCAAAAAACAATGGGAAAAACAGGATAGAATTCTTTAG
TCAGGGGGTATAG

Upstream 100 bases:

>100_bases
GAATCCTTTTGATAGCAACATATCTATTCGTTTATATATCAGTATATTACCTATGTAACATTTACTTATACAACTATATG
AATACAGAGGGGGCTTCAAG

Downstream 100 bases:

>100_bases
CTATGAAGGTATTACTGGCTTTGTTAAACATAAGTATATTTTTAGCTATAGTCATCGGGGCCAGCAAATATTTTTATTTT
ACATATCTAAAGCGTTTTAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSYCLIFLTVLIAITTGYYRFFCKLKTNKIILLAGCLMVIEINYIILKSPLTNWLFFVFIYLASISFFVKGGLIAALISI
IFTYLQSPPETGLNIYLFAGYIIMGAVTGKYSDYIHTSILKKASWQKQLYQQAKELNILREISTALQSTLKLDKLLHIIL
TAITAGYGLGFNRAILLLVSRDEKYLEGKFGIGPMNKVEGYEIWENVVAAKMNMKDFIGLKEKARLKDKTLNDLVQSLKI
PLNPSSQGGVLAEAIKYQKPIIIKSIDGNDPVQVRLASYFGMSEFAVIPLINQGKPVGAIIIDNIVNHKPITVKDLDSIT
PLAIQAAMAIDNANLYNKTQKMAITDGLTGLYNQRYFLEVIDKYFKTAREKNRHLSILIMDIDNFKNYNDVNGHLAGNTA
LSRLTDIIKKSIRKKDVPCRFGGEEFAVLFPDTTRDEAYNLAETIRKNIEKTPFYNEDVQPGGRLTVSLGLAIFPDDSQE
VKDLLDKADQALYKAKNNGKNRIEFFSQGV

Sequences:

>Translated_510_residues
MSYCLIFLTVLIAITTGYYRFFCKLKTNKIILLAGCLMVIEINYIILKSPLTNWLFFVFIYLASISFFVKGGLIAALISI
IFTYLQSPPETGLNIYLFAGYIIMGAVTGKYSDYIHTSILKKASWQKQLYQQAKELNILREISTALQSTLKLDKLLHIIL
TAITAGYGLGFNRAILLLVSRDEKYLEGKFGIGPMNKVEGYEIWENVVAAKMNMKDFIGLKEKARLKDKTLNDLVQSLKI
PLNPSSQGGVLAEAIKYQKPIIIKSIDGNDPVQVRLASYFGMSEFAVIPLINQGKPVGAIIIDNIVNHKPITVKDLDSIT
PLAIQAAMAIDNANLYNKTQKMAITDGLTGLYNQRYFLEVIDKYFKTAREKNRHLSILIMDIDNFKNYNDVNGHLAGNTA
LSRLTDIIKKSIRKKDVPCRFGGEEFAVLFPDTTRDEAYNLAETIRKNIEKTPFYNEDVQPGGRLTVSLGLAIFPDDSQE
VKDLLDKADQALYKAKNNGKNRIEFFSQGV
>Mature_509_residues
SYCLIFLTVLIAITTGYYRFFCKLKTNKIILLAGCLMVIEINYIILKSPLTNWLFFVFIYLASISFFVKGGLIAALISII
FTYLQSPPETGLNIYLFAGYIIMGAVTGKYSDYIHTSILKKASWQKQLYQQAKELNILREISTALQSTLKLDKLLHIILT
AITAGYGLGFNRAILLLVSRDEKYLEGKFGIGPMNKVEGYEIWENVVAAKMNMKDFIGLKEKARLKDKTLNDLVQSLKIP
LNPSSQGGVLAEAIKYQKPIIIKSIDGNDPVQVRLASYFGMSEFAVIPLINQGKPVGAIIIDNIVNHKPITVKDLDSITP
LAIQAAMAIDNANLYNKTQKMAITDGLTGLYNQRYFLEVIDKYFKTAREKNRHLSILIMDIDNFKNYNDVNGHLAGNTAL
SRLTDIIKKSIRKKDVPCRFGGEEFAVLFPDTTRDEAYNLAETIRKNIEKTPFYNEDVQPGGRLTVSLGLAIFPDDSQEV
KDLLDKADQALYKAKNNGKNRIEFFSQGV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=35.9281437125749, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=35.1515151515151, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=34.4632768361582, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=31.5151515151515, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=34.1317365269461, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787802, Length=196, Percent_Identity=31.6326530612245, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788381, Length=204, Percent_Identity=27.9411764705882, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=35.4285714285714, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=29.585798816568, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=29.8780487804878, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=29.5180722891566, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=179, Percent_Identity=32.4022346368715, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 57317; Mature: 57186

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYCLIFLTVLIAITTGYYRFFCKLKTNKIILLAGCLMVIEINYIILKSPLTNWLFFVFI
CHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEHHHHHHHHEECEEEECCCHHHHHHHHHH
YLASISFFVKGGLIAALISIIFTYLQSPPETGLNIYLFAGYIIMGAVTGKYSDYIHTSIL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHH
KKASWQKQLYQQAKELNILREISTALQSTLKLDKLLHIILTAITAGYGLGFNRAILLLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RDEKYLEGKFGIGPMNKVEGYEIWENVVAAKMNMKDFIGLKEKARLKDKTLNDLVQSLKI
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCC
PLNPSSQGGVLAEAIKYQKPIIIKSIDGNDPVQVRLASYFGMSEFAVIPLINQGKPVGAI
CCCCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEEEHHCCCCCEEEEEEECCCCCCEEE
IIDNIVNHKPITVKDLDSITPLAIQAAMAIDNANLYNKTQKMAITDGLTGLYNQRYFLEV
EEHHHCCCCCCEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHH
IDKYFKTAREKNRHLSILIMDIDNFKNYNDVNGHLAGNTALSRLTDIIKKSIRKKDVPCR
HHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCC
FGGEEFAVLFPDTTRDEAYNLAETIRKNIEKTPFYNEDVQPGGRLTVSLGLAIFPDDSQE
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCHHH
VKDLLDKADQALYKAKNNGKNRIEFFSQGV
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
SYCLIFLTVLIAITTGYYRFFCKLKTNKIILLAGCLMVIEINYIILKSPLTNWLFFVFI
HHHHHHHHHHHHHHHHHHEEEEEECCCCEEEHHHHHHHHEECEEEECCCHHHHHHHHHH
YLASISFFVKGGLIAALISIIFTYLQSPPETGLNIYLFAGYIIMGAVTGKYSDYIHTSIL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHH
KKASWQKQLYQQAKELNILREISTALQSTLKLDKLLHIILTAITAGYGLGFNRAILLLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
RDEKYLEGKFGIGPMNKVEGYEIWENVVAAKMNMKDFIGLKEKARLKDKTLNDLVQSLKI
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCC
PLNPSSQGGVLAEAIKYQKPIIIKSIDGNDPVQVRLASYFGMSEFAVIPLINQGKPVGAI
CCCCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEEEHHCCCCCEEEEEEECCCCCCEEE
IIDNIVNHKPITVKDLDSITPLAIQAAMAIDNANLYNKTQKMAITDGLTGLYNQRYFLEV
EEHHHCCCCCCEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHH
IDKYFKTAREKNRHLSILIMDIDNFKNYNDVNGHLAGNTALSRLTDIIKKSIRKKDVPCR
HHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCC
FGGEEFAVLFPDTTRDEAYNLAETIRKNIEKTPFYNEDVQPGGRLTVSLGLAIFPDDSQE
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCHHH
VKDLLDKADQALYKAKNNGKNRIEFFSQGV
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA