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The map label for this gene is ilvE [H]

Identifier: 220931922

GI number: 220931922

Start: 1173804

End: 1174703

Strand: Direct

Name: ilvE [H]

Synonym: Hore_10810

Alternate gene names: 220931922

Gene position: 1173804-1174703 (Clockwise)

Preceding gene: 220931921

Following gene: 220931923

Centisome position: 45.53

GC content: 37.89

Gene sequence:

>900_bases
TTGAGTAGAAAAATATATGTGAACGGGTGTATTGTAGATGAGAAGGATGCGAAGGTATCGGTATTTGACCATGGTTATTT
ATATGGAGACGGTATATTTGAGGGTATAAGGGCTTACAATGGCCGTGTTTTTAAATTAAAAGAGCATATTGAGAGGTTAT
ATAAATCTGCTAAAACCATCATGCTCGAGATACCCCTTACCCCTGAAGAAATGGAAGAAGCTATTTTAGAAACAATAAGG
GCCAATAATTTAATGGATGCCTATATAAGGGTTGTCATATCCAGGGGGGTCGGAGACCTGGGTTTAGATCCCAGAAAGTG
TAAGAAACCAACTGTAGTTATTATTGCCAGTAAAATAAAACTGTATGATCAGGAATTATATGAAAAGGGACTTAAGGTAG
TTACCATACCTACCCGGAGAAATGGACCGGAAATGGTTAATCCCAGAGTAAAGTCCCTGAATTATTTAAATAATATTATG
GCCAGGATTGAAGCCAATCTGGCCGGTGCTCCTGAAGCAATTATTTTGAACGATGAGGGTTATGTAGCTGAATGTACCGG
TGATAATATCTTTATTATTGAAGGTGATGAACTTATTACCCCACCGAAATATGCCGGTATTTTAAGGGGTATAAAGAGGG
ATGTGGCTATGGAAATAGCCAGGGACCTGGGTTTAAATGTTAAGGAAGAATTATTTACAAGGTATGATGTCTTTAATGCT
GATGAATGTTTCCTGACTGGAACTGCTGCAGAAGTAATACCGGTTATTGAAGTTGATGGAAGACAGGTTGGTGAAGGAAA
ACCGGGTAAATATACCAAAAAAATAATAGAAGAATTTAGGCGGATTGCAAATTCTACAGGAGTTACCATTTATCCCAAAA
ATGAAGTCAGGGTATCATAG

Upstream 100 bases:

>100_bases
ACCGGGGAGGTGAAAAAATAAACCTACTTTGTATTATGGAGTTGTAAGGGTTTGTTTCCTTTAAGCAAGTAATTAACAAT
TAAGAGGAGGAGTAGAGAAG

Downstream 100 bases:

>100_bases
AGAGTAGAAAAGTAGCAAAGGAGGTTGTTTTATTGGGGAGTGAAACAATAACACAAGGTTTTAAGAGGGCCCCCCATAGA
TCTTTATTATATGCGTTAGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSRKIYVNGCIVDEKDAKVSVFDHGYLYGDGIFEGIRAYNGRVFKLKEHIERLYKSAKTIMLEIPLTPEEMEEAILETIR
ANNLMDAYIRVVISRGVGDLGLDPRKCKKPTVVIIASKIKLYDQELYEKGLKVVTIPTRRNGPEMVNPRVKSLNYLNNIM
ARIEANLAGAPEAIILNDEGYVAECTGDNIFIIEGDELITPPKYAGILRGIKRDVAMEIARDLGLNVKEELFTRYDVFNA
DECFLTGTAAEVIPVIEVDGRQVGEGKPGKYTKKIIEEFRRIANSTGVTIYPKNEVRVS

Sequences:

>Translated_299_residues
MSRKIYVNGCIVDEKDAKVSVFDHGYLYGDGIFEGIRAYNGRVFKLKEHIERLYKSAKTIMLEIPLTPEEMEEAILETIR
ANNLMDAYIRVVISRGVGDLGLDPRKCKKPTVVIIASKIKLYDQELYEKGLKVVTIPTRRNGPEMVNPRVKSLNYLNNIM
ARIEANLAGAPEAIILNDEGYVAECTGDNIFIIEGDELITPPKYAGILRGIKRDVAMEIARDLGLNVKEELFTRYDVFNA
DECFLTGTAAEVIPVIEVDGRQVGEGKPGKYTKKIIEEFRRIANSTGVTIYPKNEVRVS
>Mature_298_residues
SRKIYVNGCIVDEKDAKVSVFDHGYLYGDGIFEGIRAYNGRVFKLKEHIERLYKSAKTIMLEIPLTPEEMEEAILETIRA
NNLMDAYIRVVISRGVGDLGLDPRKCKKPTVVIIASKIKLYDQELYEKGLKVVTIPTRRNGPEMVNPRVKSLNYLNNIMA
RIEANLAGAPEAIILNDEGYVAECTGDNIFIIEGDELITPPKYAGILRGIKRDVAMEIARDLGLNVKEELFTRYDVFNAD
ECFLTGTAAEVIPVIEVDGRQVGEGKPGKYTKKIIEEFRRIANSTGVTIYPKNEVRVS

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=281, Percent_Identity=38.0782918149466, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1787338, Length=245, Percent_Identity=26.530612244898, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33579; Mature: 33448

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKIYVNGCIVDEKDAKVSVFDHGYLYGDGIFEGIRAYNGRVFKLKEHIERLYKSAKTI
CCCEEEEEEEEEECCCCEEEEEECCEEECCHHHHHHHHHCCEEEEHHHHHHHHHHCCCEE
MLEIPLTPEEMEEAILETIRANNLMDAYIRVVISRGVGDLGLDPRKCKKPTVVIIASKIK
EEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECHHH
LYDQELYEKGLKVVTIPTRRNGPEMVNPRVKSLNYLNNIMARIEANLAGAPEAIILNDEG
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
YVAECTGDNIFIIEGDELITPPKYAGILRGIKRDVAMEIARDLGLNVKEELFTRYDVFNA
CEEEECCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
DECFLTGTAAEVIPVIEVDGRQVGEGKPGKYTKKIIEEFRRIANSTGVTIYPKNEVRVS
CCEEEECCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEC
>Mature Secondary Structure 
SRKIYVNGCIVDEKDAKVSVFDHGYLYGDGIFEGIRAYNGRVFKLKEHIERLYKSAKTI
CCEEEEEEEEEECCCCEEEEEECCEEECCHHHHHHHHHCCEEEEHHHHHHHHHHCCCEE
MLEIPLTPEEMEEAILETIRANNLMDAYIRVVISRGVGDLGLDPRKCKKPTVVIIASKIK
EEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECHHH
LYDQELYEKGLKVVTIPTRRNGPEMVNPRVKSLNYLNNIMARIEANLAGAPEAIILNDEG
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
YVAECTGDNIFIIEGDELITPPKYAGILRGIKRDVAMEIARDLGLNVKEELFTRYDVFNA
CEEEECCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
DECFLTGTAAEVIPVIEVDGRQVGEGKPGKYTKKIIEEFRRIANSTGVTIYPKNEVRVS
CCEEEECCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]