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The map label for this gene is nth [H]

Identifier: 220931920

GI number: 220931920

Start: 1171961

End: 1172599

Strand: Direct

Name: nth [H]

Synonym: Hore_10790

Alternate gene names: 220931920

Gene position: 1171961-1172599 (Clockwise)

Preceding gene: 220931918

Following gene: 220931921

Centisome position: 45.46

GC content: 38.03

Gene sequence:

>639_bases
ATGGCAAGTTATCCTTATTTACAGGAACTAATAAAATACTTTGAAGATAGATACCCTGCTCCCGATACTGAACTAAATTT
TTCTACTCCCTTTGAATTGCTTATCGCTACAATACTTTCTGCCCAGTCAACGGACAGGCAGGTTAATAAGGTTACAAAAA
AATTATTTAAGAAGTATAAAAACCCCGGGGATTTTGCTTCCCTGGATAGAAAAACCCTGGAAAGAGAAATTAACAGTATC
GGATTATACCGCAACAAAAGTAAATACATTATTGAGGTCAGTAACATTTTGATAAAAGAATATGGAGGTAAAGTTCCTGG
TACCAGAAAAGAGCTTTTAAAATTACCCGGTGTTGGTAGGAAAACTGCCAATGTTGTTCTGGCCTGTGCCTTTAACAAAA
AAACTTTTCCGGTTGATACTCATGTCTTCAGGATTTCTAACCGGTTGGGACTGGTGAGTGCTAAAAGGACTAACGAAGCT
GAAAAGCAACTGATGGAAGTTATTCCTGAAGAGAAATGGGTTGATATGCACCACTGGCTTATCTTTCATGGCAGGGAAGT
CTGTAAAGCCAGGAATCCCGCCTGTCATTTCTGTGAGCTTAAACCATTCTGCAATTATTATAAAAAGGAGAATGATTAG

Upstream 100 bases:

>100_bases
ATTTACCTGGATTTAGTGAAAAATTGTTATTCTAAAATAAATATACATCAAAAATATGATAACATTTTTTGGGGCTTAAG
TCCAGAGAGGGAGTCTTACT

Downstream 100 bases:

>100_bases
TTTTGGTATAAACACTTTTTAATTCTTTATACCTTAAATGAGAATTAGAAATTGATTTAAATATATAGAAATTAAATATA
TTTGGTACTTACACAGGTAA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MASYPYLQELIKYFEDRYPAPDTELNFSTPFELLIATILSAQSTDRQVNKVTKKLFKKYKNPGDFASLDRKTLEREINSI
GLYRNKSKYIIEVSNILIKEYGGKVPGTRKELLKLPGVGRKTANVVLACAFNKKTFPVDTHVFRISNRLGLVSAKRTNEA
EKQLMEVIPEEKWVDMHHWLIFHGREVCKARNPACHFCELKPFCNYYKKEND

Sequences:

>Translated_212_residues
MASYPYLQELIKYFEDRYPAPDTELNFSTPFELLIATILSAQSTDRQVNKVTKKLFKKYKNPGDFASLDRKTLEREINSI
GLYRNKSKYIIEVSNILIKEYGGKVPGTRKELLKLPGVGRKTANVVLACAFNKKTFPVDTHVFRISNRLGLVSAKRTNEA
EKQLMEVIPEEKWVDMHHWLIFHGREVCKARNPACHFCELKPFCNYYKKEND
>Mature_211_residues
ASYPYLQELIKYFEDRYPAPDTELNFSTPFELLIATILSAQSTDRQVNKVTKKLFKKYKNPGDFASLDRKTLEREINSIG
LYRNKSKYIIEVSNILIKEYGGKVPGTRKELLKLPGVGRKTANVVLACAFNKKTFPVDTHVFRISNRLGLVSAKRTNEAE
KQLMEVIPEEKWVDMHHWLIFHGREVCKARNPACHFCELKPFCNYYKKEND

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=46.7661691542289, Blast_Score=191, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17554540, Length=183, Percent_Identity=31.6939890710383, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319304, Length=129, Percent_Identity=32.5581395348837, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=25, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=34.2696629213483, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24626; Mature: 24495

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASYPYLQELIKYFEDRYPAPDTELNFSTPFELLIATILSAQSTDRQVNKVTKKLFKKYK
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
NPGDFASLDRKTLEREINSIGLYRNKSKYIIEVSNILIKEYGGKVPGTRKELLKLPGVGR
CCCCHHHHHHHHHHHHHHHHCCEECCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
KTANVVLACAFNKKTFPVDTHVFRISNRLGLVSAKRTNEAEKQLMEVIPEEKWVDMHHWL
CCCCEEEEEEECCCCCCCCHHHEEECCCCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHH
IFHGREVCKARNPACHFCELKPFCNYYKKEND
HHHHHHHHHCCCCCCCEECCCHHHHHHHCCCC
>Mature Secondary Structure 
ASYPYLQELIKYFEDRYPAPDTELNFSTPFELLIATILSAQSTDRQVNKVTKKLFKKYK
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
NPGDFASLDRKTLEREINSIGLYRNKSKYIIEVSNILIKEYGGKVPGTRKELLKLPGVGR
CCCCHHHHHHHHHHHHHHHHCCEECCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
KTANVVLACAFNKKTFPVDTHVFRISNRLGLVSAKRTNEAEKQLMEVIPEEKWVDMHHWL
CCCCEEEEEEECCCCCCCCHHHEEECCCCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHH
IFHGREVCKARNPACHFCELKPFCNYYKKEND
HHHHHHHHHCCCCCCCEECCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]