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The map label for this gene is thrS [H]

Identifier: 220931918

GI number: 220931918

Start: 1169538

End: 1171187

Strand: Direct

Name: thrS [H]

Synonym: Hore_10770

Alternate gene names: 220931918

Gene position: 1169538-1171187 (Clockwise)

Preceding gene: 220931917

Following gene: 220931920

Centisome position: 45.36

GC content: 33.03

Gene sequence:

>1650_bases
ATGATTGAAGTCTATATTAATGAAAAAAAATACAGTTTTGATAAGGGTATAACCGTTGAGGAGGTTGTAAAGGAAACATA
CAATGGAAATTATAGAGAAGTAGTGGGGGCAATTGTAAATAATGAAGTAGAATCGTTAAATTACAAATTAAATAACCGGA
GTCAGGTTTCTTTTCTTACAATTAATGACGAAAGTGGTAACAGAATTTACCGGAGAAGTTTGTTTTTTCTATTGACCAGG
GTTATATATTATTTGTATCCCGGAGCCAAGTTATCCATAGAGCATTCTCTGGGAAATGGAATTTATTGTGAATTAAATAA
AAATACACCCATAACCCAGTCAGATCTTATTAAAATAAAAAAGAGAATGGAAGAACTGGTGAGAAAAGACCTGCCTATCA
ATAAAATAAAGGTAAACAAAGATAAACTGGAGAAAATTTATACAGAGCAGGATTTTTATGATAAAGTTAAGCTAGTTAAA
CAATTAGATATCTCTCAGTGGCCTGTATATGAGCTCGATGGATTTTATAACTATTTTTATAGTATTCTGGTTCCCAGTAC
CGGTTATCTGGATAAATTCGATATCCACTTTAAAATGCCGGGATTTATTCTATTATATCCTTTTCCCGGTTCTAATAAAG
AGGTTTCAAGGTATGTAGAGCAACCAAAACTTGCCAGTATATTTTATGAATATGAAAAATGGGGAGAGATTATAAATGTA
AGTAATGTCAGTGAATTAAACGAAGTAATTACTAATAATGATTATGGGGATATTATCAGAATTACAGAAGCATTACATGA
AAAAAAGATCGCTTTAATTGCAGATGAAATTACAGAAAAAATAGATAATAAAAGGGTTATTTTGATTGCTGGCCCATCTT
CATCCGGGAAAACTACTTTTGCCCAGAGATTATCAATTCAACTCAGGGTAAATGGATTAAGGCCAGTTGCCATTTCCACC
GATGATTATTTTGTTGACCGTGATAAAACGCCACGGGATGAAGAAGGGAACCTTGATTTTGAAGCTATAGAAGCTATTGA
TTTGAAATTATTTAATGAACATCTGGTAAAACTTCTCCAGGGTGAAGAGGTAGAAATACCGATCTATAATTTTAAAAAGG
GAAAGAGGGAACCTGTAGGCAGGCATCTAAAGATTGAGAAAGACCAGCCAATAATTATTGAAGGAATTCACGGTCTTAAT
GAAAGGTTAACAGCTGTAATTCCTAAGGAATTAAAATATAAAATATATGTAAGTGCCCTGACCCAGTTAAATATAGACCG
TCATAACCGGATTCCAACTACCGATAATAGATTAATAAGGAGAATTGTTAGAGATTATTATTTCAGGGGCCATGACGCTG
CTAAAACAATTGAATTATGGCCCGCTGTCAGGAGAGGGGAAGAAAAAAACATCTTCCCATACCAGGAAAATGCCGATATT
ATGTTTAATTCTGCCCTGACCTATGAACTTTCTGTATTGAAAAAATATGCCTTTCCTATTTTAAAAGAAATAAAATCAGG
TCAGGATGGTTATTTTGAAGCAAAAAGATTACTGGAAATATTATCGTGTTTTAATAGTTTACCTGATGAAAAAGATATTC
CCAGAACATCTATTTTAAGGGAGTTTATAGGGGGGAGTTGTTTTAAATAA

Upstream 100 bases:

>100_bases
GAAATCAGAAAAAGATAAGGTTGTAGAAAATTATTTTAAAAAGGTTATAAAAAGATTAAATCAATAAATATTTTATGGTT
ATTAGGCCAGGAGGTATATT

Downstream 100 bases:

>100_bases
TTCAAATATCTAACCGGCCCTCTCTTTATCTTTCTCTATATTAAAGGTTCTGATGATAATTTCATAGGCCCTTTCCCAGT
TGTTAACACGATATAATTTT

Product: uridine kinase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MIEVYINEKKYSFDKGITVEEVVKETYNGNYREVVGAIVNNEVESLNYKLNNRSQVSFLTINDESGNRIYRRSLFFLLTR
VIYYLYPGAKLSIEHSLGNGIYCELNKNTPITQSDLIKIKKRMEELVRKDLPINKIKVNKDKLEKIYTEQDFYDKVKLVK
QLDISQWPVYELDGFYNYFYSILVPSTGYLDKFDIHFKMPGFILLYPFPGSNKEVSRYVEQPKLASIFYEYEKWGEIINV
SNVSELNEVITNNDYGDIIRITEALHEKKIALIADEITEKIDNKRVILIAGPSSSGKTTFAQRLSIQLRVNGLRPVAIST
DDYFVDRDKTPRDEEGNLDFEAIEAIDLKLFNEHLVKLLQGEEVEIPIYNFKKGKREPVGRHLKIEKDQPIIIEGIHGLN
ERLTAVIPKELKYKIYVSALTQLNIDRHNRIPTTDNRLIRRIVRDYYFRGHDAAKTIELWPAVRRGEEKNIFPYQENADI
MFNSALTYELSVLKKYAFPILKEIKSGQDGYFEAKRLLEILSCFNSLPDEKDIPRTSILREFIGGSCFK

Sequences:

>Translated_549_residues
MIEVYINEKKYSFDKGITVEEVVKETYNGNYREVVGAIVNNEVESLNYKLNNRSQVSFLTINDESGNRIYRRSLFFLLTR
VIYYLYPGAKLSIEHSLGNGIYCELNKNTPITQSDLIKIKKRMEELVRKDLPINKIKVNKDKLEKIYTEQDFYDKVKLVK
QLDISQWPVYELDGFYNYFYSILVPSTGYLDKFDIHFKMPGFILLYPFPGSNKEVSRYVEQPKLASIFYEYEKWGEIINV
SNVSELNEVITNNDYGDIIRITEALHEKKIALIADEITEKIDNKRVILIAGPSSSGKTTFAQRLSIQLRVNGLRPVAIST
DDYFVDRDKTPRDEEGNLDFEAIEAIDLKLFNEHLVKLLQGEEVEIPIYNFKKGKREPVGRHLKIEKDQPIIIEGIHGLN
ERLTAVIPKELKYKIYVSALTQLNIDRHNRIPTTDNRLIRRIVRDYYFRGHDAAKTIELWPAVRRGEEKNIFPYQENADI
MFNSALTYELSVLKKYAFPILKEIKSGQDGYFEAKRLLEILSCFNSLPDEKDIPRTSILREFIGGSCFK
>Mature_549_residues
MIEVYINEKKYSFDKGITVEEVVKETYNGNYREVVGAIVNNEVESLNYKLNNRSQVSFLTINDESGNRIYRRSLFFLLTR
VIYYLYPGAKLSIEHSLGNGIYCELNKNTPITQSDLIKIKKRMEELVRKDLPINKIKVNKDKLEKIYTEQDFYDKVKLVK
QLDISQWPVYELDGFYNYFYSILVPSTGYLDKFDIHFKMPGFILLYPFPGSNKEVSRYVEQPKLASIFYEYEKWGEIINV
SNVSELNEVITNNDYGDIIRITEALHEKKIALIADEITEKIDNKRVILIAGPSSSGKTTFAQRLSIQLRVNGLRPVAIST
DDYFVDRDKTPRDEEGNLDFEAIEAIDLKLFNEHLVKLLQGEEVEIPIYNFKKGKREPVGRHLKIEKDQPIIIEGIHGLN
ERLTAVIPKELKYKIYVSALTQLNIDRHNRIPTTDNRLIRRIVRDYYFRGHDAAKTIELWPAVRRGEEKNIFPYQENADI
MFNSALTYELSVLKKYAFPILKEIKSGQDGYFEAKRLLEILSCFNSLPDEKDIPRTSILREFIGGSCFK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0572

COG function: function code F; Uridine kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788013, Length=149, Percent_Identity=26.8456375838926, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 63926; Mature: 63926

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVYINEKKYSFDKGITVEEVVKETYNGNYREVVGAIVNNEVESLNYKLNNRSQVSFLT
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCEEEECCCCEEEEEE
INDESGNRIYRRSLFFLLTRVIYYLYPGAKLSIEHSLGNGIYCELNKNTPITQSDLIKIK
EECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHH
KRMEELVRKDLPINKIKVNKDKLEKIYTEQDFYDKVKLVKQLDISQWPVYELDGFYNYFY
HHHHHHHHHCCCCCEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
SILVPSTGYLDKFDIHFKMPGFILLYPFPGSNKEVSRYVEQPKLASIFYEYEKWGEIINV
HHHCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCEECC
SNVSELNEVITNNDYGDIIRITEALHEKKIALIADEITEKIDNKRVILIAGPSSSGKTTF
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHE
AQRLSIQLRVNGLRPVAISTDDYFVDRDKTPRDEEGNLDFEAIEAIDLKLFNEHLVKLLQ
EEEEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GEEVEIPIYNFKKGKREPVGRHLKIEKDQPIIIEGIHGLNERLTAVIPKELKYKIYVSAL
CCCEEEEEECCCCCCCCCCCCCEEECCCCCEEEECCCCCHHHHHHHCCHHHEEEEEEEEH
TQLNIDRHNRIPTTDNRLIRRIVRDYYFRGHDAAKTIELWPAVRRGEEKNIFPYQENADI
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
MFNSALTYELSVLKKYAFPILKEIKSGQDGYFEAKRLLEILSCFNSLPDEKDIPRTSILR
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
EFIGGSCFK
HHHCCCCCC
>Mature Secondary Structure
MIEVYINEKKYSFDKGITVEEVVKETYNGNYREVVGAIVNNEVESLNYKLNNRSQVSFLT
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCEEEECCCCEEEEEE
INDESGNRIYRRSLFFLLTRVIYYLYPGAKLSIEHSLGNGIYCELNKNTPITQSDLIKIK
EECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHH
KRMEELVRKDLPINKIKVNKDKLEKIYTEQDFYDKVKLVKQLDISQWPVYELDGFYNYFY
HHHHHHHHHCCCCCEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
SILVPSTGYLDKFDIHFKMPGFILLYPFPGSNKEVSRYVEQPKLASIFYEYEKWGEIINV
HHHCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCEECC
SNVSELNEVITNNDYGDIIRITEALHEKKIALIADEITEKIDNKRVILIAGPSSSGKTTF
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHE
AQRLSIQLRVNGLRPVAISTDDYFVDRDKTPRDEEGNLDFEAIEAIDLKLFNEHLVKLLQ
EEEEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GEEVEIPIYNFKKGKREPVGRHLKIEKDQPIIIEGIHGLNERLTAVIPKELKYKIYVSAL
CCCEEEEEECCCCCCCCCCCCCEEECCCCCEEEECCCCCHHHHHHHCCHHHEEEEEEEEH
TQLNIDRHNRIPTTDNRLIRRIVRDYYFRGHDAAKTIELWPAVRRGEEKNIFPYQENADI
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
MFNSALTYELSVLKKYAFPILKEIKSGQDGYFEAKRLLEILSCFNSLPDEKDIPRTSILR
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
EFIGGSCFK
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA