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The map label for this gene is cht60 [H]

Identifier: 221230859

GI number: 221230859

Start: 3063826

End: 3064989

Strand: Reverse

Name: cht60 [H]

Synonym: MLBr_02569

Alternate gene names: 221230859

Gene position: 3064989-3063826 (Counterclockwise)

Preceding gene: 221230863

Following gene: 221230858

Centisome position: 93.79

GC content: 63.83

Gene sequence:

>1164_bases
ATGGCTTTTCCGCGCACTCTGATTGTGCTTGCTGCCGCCTCGGCCCTGGTGGTGACTTGTGGCCACGATGTTGCACAGGC
TCCTGACTCGGCGTCGATCGTAGCCGGGGAGCTTACCGCCGCCGCGCTGACACCGGCGTGCGTCGATCTCCCGGCATCTG
TGCCGGGGACCCTGTCCACCCGCGACAAGCTGGCCCAGCTGCTGATGGTAGGCGTACGTGATGCGGCCGATGCCAAGGCC
GTTGTCACCGATCACCACGTCGGCGGCATCATGATCGGCAGCTGGACAGACTTGTCGATGTTGATCAACGGCGCGCTGAC
AGACATCGCCCGCAATCCGGGACCGCTGCCGCTGGCGGTCAGCGTCGATGAAGAAGGTGGTCGGGTGTCGCGGTTGAAGT
CGCTGATAGGTATGGCGCCTTCGGCTCGGGTGCTGGCACAGACCAGCACTGTTGACCAGGTCTACGACATGGCGCTGGAC
CGTGGTCACAAGATGCGCGATTTGGGGATCACCATCGATTTCGCCCCAGTCGTCGACGTGACCGACGCCCCGGACGACAG
CGTTATCGGTGGCCGCTCATTTAGCTCGGACCCGGCCGTCGTTACAGCCTACGCTGGGGCGTACGCGCGGGGTCTGCGTG
ACGCTGGCGTGCTGCCTGTACTCAAACATTTTCCCGGTCATGGGCATGGTTCGGGAGATTCGCACACTGGCGGTGTCACC
ACGCCGCCGCTGCAAGACCTGCAAACCAACGACTTGGTTCCTTACCGGACGTTGGTGGCCGATGCCCCGGTCGGAGTGAT
GCTCGGTCATCTGGACGTTCCCGGGCTGACCGGTGACGACCCAGCGAGTTTGAGCCCCGCCGCGGTGCAGTTGCTGCGCA
CTGGCGCTAACTACGGGGCCCCACCTTTCAATGGTCCGGTCTTTAGTGACGATGTGTCCAGCATGGCTGCGATTTCTGAC
CGCTACGGGCTGGCTGACGCTGTGCTACGCACCTTGCAAGCTGGCACCGACATCGCGCTGTGGGTCAGCACCCAGACAGT
GCCTGCAGTCTTGGACCGGCTAGAGAAGGTGGTATCCGCGGGCGAATTAGCGATGTCGGCCGTCGACGCTTCAGTCGTGC
GGGTAGCCACCATAAAAGGTCCCGGCCCGGGGTGCGGGCGCTAG

Upstream 100 bases:

>100_bases
GAGGACCGATCAGCATGAACGCGGTCGTCACCCCACCCCGGTCAGCGGGTGCCAGGTGGGGTGGGGCGCGTACGAATACC
GGCCATGACACCATGGTCTG

Downstream 100 bases:

>100_bases
CACAGTCTGCGTGGCAGCTGCGTCGGTGTTTACTCTGGAGTAAGAGCGTCCACGCTAAAGGGGCATGCGATGGCAGGTGG
TGCAAAGAGGATACCGCGTG

Product: putative secreted glycosyl hydrolase

Products: NA

Alternate protein names: Beta-N-acetylhexosaminidase; Chitobiase; N-acetyl-beta-glucosaminidase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAFPRTLIVLAAASALVVTCGHDVAQAPDSASIVAGELTAAALTPACVDLPASVPGTLSTRDKLAQLLMVGVRDAADAKA
VVTDHHVGGIMIGSWTDLSMLINGALTDIARNPGPLPLAVSVDEEGGRVSRLKSLIGMAPSARVLAQTSTVDQVYDMALD
RGHKMRDLGITIDFAPVVDVTDAPDDSVIGGRSFSSDPAVVTAYAGAYARGLRDAGVLPVLKHFPGHGHGSGDSHTGGVT
TPPLQDLQTNDLVPYRTLVADAPVGVMLGHLDVPGLTGDDPASLSPAAVQLLRTGANYGAPPFNGPVFSDDVSSMAAISD
RYGLADAVLRTLQAGTDIALWVSTQTVPAVLDRLEKVVSAGELAMSAVDASVVRVATIKGPGPGCGR

Sequences:

>Translated_387_residues
MAFPRTLIVLAAASALVVTCGHDVAQAPDSASIVAGELTAAALTPACVDLPASVPGTLSTRDKLAQLLMVGVRDAADAKA
VVTDHHVGGIMIGSWTDLSMLINGALTDIARNPGPLPLAVSVDEEGGRVSRLKSLIGMAPSARVLAQTSTVDQVYDMALD
RGHKMRDLGITIDFAPVVDVTDAPDDSVIGGRSFSSDPAVVTAYAGAYARGLRDAGVLPVLKHFPGHGHGSGDSHTGGVT
TPPLQDLQTNDLVPYRTLVADAPVGVMLGHLDVPGLTGDDPASLSPAAVQLLRTGANYGAPPFNGPVFSDDVSSMAAISD
RYGLADAVLRTLQAGTDIALWVSTQTVPAVLDRLEKVVSAGELAMSAVDASVVRVATIKGPGPGCGR
>Mature_386_residues
AFPRTLIVLAAASALVVTCGHDVAQAPDSASIVAGELTAAALTPACVDLPASVPGTLSTRDKLAQLLMVGVRDAADAKAV
VTDHHVGGIMIGSWTDLSMLINGALTDIARNPGPLPLAVSVDEEGGRVSRLKSLIGMAPSARVLAQTSTVDQVYDMALDR
GHKMRDLGITIDFAPVVDVTDAPDDSVIGGRSFSSDPAVVTAYAGAYARGLRDAGVLPVLKHFPGHGHGSGDSHTGGVTT
PPLQDLQTNDLVPYRTLVADAPVGVMLGHLDVPGLTGDDPASLSPAAVQLLRTGANYGAPPFNGPVFSDDVSSMAAISDR
YGLADAVLRTLQAGTDIALWVSTQTVPAVLDRLEKVVSAGELAMSAVDASVVRVATIKGPGPGCGR

Specific function: Most active towards p-nitrophenyl-N-acetyl-beta-D- glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=246, Percent_Identity=32.520325203252, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.52 [H]

Molecular weight: Translated: 39510; Mature: 39379

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFPRTLIVLAAASALVVTCGHDVAQAPDSASIVAGELTAAALTPACVDLPASVPGTLST
CCCCHHHHHHHHHHHEEEECCCHHHCCCCCCCEEEHHHHHHHHCHHHHCCCCCCCCCCCH
RDKLAQLLMVGVRDAADAKAVVTDHHVGGIMIGSWTDLSMLINGALTDIARNPGPLPLAV
HHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEE
SVDEEGGRVSRLKSLIGMAPSARVLAQTSTVDQVYDMALDRGHKMRDLGITIDFAPVVDV
EECCCCCHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHCCCCEEECEEEEEECCEEEC
TDAPDDSVIGGRSFSSDPAVVTAYAGAYARGLRDAGVLPVLKHFPGHGHGSGDSHTGGVT
CCCCCCCEECCCCCCCCCCEEEEHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
TPPLQDLQTNDLVPYRTLVADAPVGVMLGHLDVPGLTGDDPASLSPAAVQLLRTGANYGA
CCCHHCCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
PPFNGPVFSDDVSSMAAISDRYGLADAVLRTLQAGTDIALWVSTQTVPAVLDRLEKVVSA
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHH
GELAMSAVDASVVRVATIKGPGPGCGR
HHHHHHHHCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
AFPRTLIVLAAASALVVTCGHDVAQAPDSASIVAGELTAAALTPACVDLPASVPGTLST
CCCHHHHHHHHHHHEEEECCCHHHCCCCCCCEEEHHHHHHHHCHHHHCCCCCCCCCCCH
RDKLAQLLMVGVRDAADAKAVVTDHHVGGIMIGSWTDLSMLINGALTDIARNPGPLPLAV
HHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEE
SVDEEGGRVSRLKSLIGMAPSARVLAQTSTVDQVYDMALDRGHKMRDLGITIDFAPVVDV
EECCCCCHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHCCCCEEECEEEEEECCEEEC
TDAPDDSVIGGRSFSSDPAVVTAYAGAYARGLRDAGVLPVLKHFPGHGHGSGDSHTGGVT
CCCCCCCEECCCCCCCCCCEEEEHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
TPPLQDLQTNDLVPYRTLVADAPVGVMLGHLDVPGLTGDDPASLSPAAVQLLRTGANYGA
CCCHHCCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
PPFNGPVFSDDVSSMAAISDRYGLADAVLRTLQAGTDIALWVSTQTVPAVLDRLEKVVSA
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHH
GELAMSAVDASVVRVATIKGPGPGCGR
HHHHHHHHCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063094 [H]