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The map label for this gene is hemB

Identifier: 221230778

GI number: 221230778

Start: 2893697

End: 2894686

Strand: Reverse

Name: hemB

Synonym: MLBr_02419

Alternate gene names: 221230778

Gene position: 2894686-2893697 (Counterclockwise)

Preceding gene: 221230779

Following gene: 221230777

Centisome position: 88.57

GC content: 61.41

Gene sequence:

>990_bases
GTGAGCGTCAGTGGTTACCCGCGGCACCGGCCGCGCCGGCTTCGCTCAACCCCCGCGATGCGCCGCCTGGTGGCGCAGAC
TTCTCTGGAGCCAAGGAATTTGGTGCTGCCGATGTTCGTTGCTGACGGTATTGACGAGCTGCGGCCAATTGCTTCCATGC
CGGGTGTTGTGCAGCACACCCGGGATTCGCTGCGTCGCGCTGCTGTCGCTGCCGTCGATGCCGGGGTAGGTGGATTGAAC
CTGTTTGGCGTGCCCCGCGACCAGGATAAGGATGCAACTGGCTCGGCTGGGGTAGATCCCGATGGCATCCTCAATGTCGC
TCTTCGCGATCTGGCCGAGGATCTCGGTGATGCCACCGTGTTGATGGCCGACACCTGTCTGGACGAGTTTACCGACCACG
GGCACTGCGGCGTCCTAGACGGGCAGGGCCGAGTGGACAACGACGCCACCGTGGCACGGTATGTGGAACTTGCTGTGGCA
CAAGCGGAATCCGGTGCTAACGTGGTTGGTCCGAGCGGGATGATGGACGGCCAAATAGGTGCGCTCCGAGACGGACTTGA
CAGTGCAGGCTATGCCGACGTAGCGATCCTGGCTTACGCTGCGAAGTTTTCTTCGGCGTTCTACGGACCATTCCGCGAGG
CGGTGAGCTGCAGCTTGTCCGGTGACCGACGCACCTACCAGCAGGAACCAGGCAATGCCCGCGAGGCGTTGCGCGAAATC
AAGTTGGATCTCGACGAGGGTGCAGACATTATCATGATCAAACCCGCTTCGGGGTATCTGGACGTCGTTGCTACGGCAGC
CGGTGTTTCGCCGGTTCCGGTAGCTGCCTATCAAGTCTCGGGGGAGTACGCGATGATTTGCGCTGCGGCCGCAAACAACT
GGATTGACGAACGTGCCGCTGTGCTGGAGTCGTTGACAAGTATCCGGCGCGCTGGGGCGGATATCGTGTTCACCTATTGG
GCCGCTGATGTGGCGTGTTGGCTTTCTTGA

Upstream 100 bases:

>100_bases
CTGCGCGCCGAAGGCGCGCTGCCTCCACCGCGCAAGAAGAGCCGCAGGCGCTAGTAGTGATCGTGAGCACGGTTAGGACG
GGCGCGGCAGTAGGCAGTCA

Downstream 100 bases:

>100_bases
CGAGTCTATAGGCCACAATCACGAGATGGGGCTGTAGATGACGGCAAAAACGGAGGCCAATCGCAAGCCTTTGTTTTATG
AGTCCGGCGCCAACTGGATC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSVSGYPRHRPRRLRSTPAMRRLVAQTSLEPRNLVLPMFVADGIDELRPIASMPGVVQHTRDSLRRAAVAAVDAGVGGLN
LFGVPRDQDKDATGSAGVDPDGILNVALRDLAEDLGDATVLMADTCLDEFTDHGHCGVLDGQGRVDNDATVARYVELAVA
QAESGANVVGPSGMMDGQIGALRDGLDSAGYADVAILAYAAKFSSAFYGPFREAVSCSLSGDRRTYQQEPGNAREALREI
KLDLDEGADIIMIKPASGYLDVVATAAGVSPVPVAAYQVSGEYAMICAAAANNWIDERAAVLESLTSIRRAGADIVFTYW
AADVACWLS

Sequences:

>Translated_329_residues
MSVSGYPRHRPRRLRSTPAMRRLVAQTSLEPRNLVLPMFVADGIDELRPIASMPGVVQHTRDSLRRAAVAAVDAGVGGLN
LFGVPRDQDKDATGSAGVDPDGILNVALRDLAEDLGDATVLMADTCLDEFTDHGHCGVLDGQGRVDNDATVARYVELAVA
QAESGANVVGPSGMMDGQIGALRDGLDSAGYADVAILAYAAKFSSAFYGPFREAVSCSLSGDRRTYQQEPGNAREALREI
KLDLDEGADIIMIKPASGYLDVVATAAGVSPVPVAAYQVSGEYAMICAAAANNWIDERAAVLESLTSIRRAGADIVFTYW
AADVACWLS
>Mature_328_residues
SVSGYPRHRPRRLRSTPAMRRLVAQTSLEPRNLVLPMFVADGIDELRPIASMPGVVQHTRDSLRRAAVAAVDAGVGGLNL
FGVPRDQDKDATGSAGVDPDGILNVALRDLAEDLGDATVLMADTCLDEFTDHGHCGVLDGQGRVDNDATVARYVELAVAQ
AESGANVVGPSGMMDGQIGALRDGLDSAGYADVAILAYAAKFSSAFYGPFREAVSCSLSGDRRTYQQEPGNAREALREIK
LDLDEGADIIMIKPASGYLDVVATAAGVSPVPVAAYQVSGEYAMICAAAANNWIDERAAVLESLTSIRRAGADIVFTYWA
ADVACWLS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family

Homologues:

Organism=Homo sapiens, GI189083849, Length=309, Percent_Identity=40.453074433657, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=46.6876971608833, Blast_Score=279, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321398, Length=317, Percent_Identity=36.9085173501577, Blast_Score=202, Evalue=8e-53,
Organism=Drosophila melanogaster, GI21358291, Length=294, Percent_Identity=39.7959183673469, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM2_MYCLE (P46723)

Other databases:

- EMBL:   U00018
- EMBL:   AL583925
- PIR:   S72910
- RefSeq:   NP_302564.1
- ProteinModelPortal:   P46723
- SMR:   P46723
- EnsemblBacteria:   EBMYCT00000029189
- GeneID:   908581
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2419
- NMPDR:   fig|272631.1.peg.1436
- Leproma:   ML2419
- GeneTree:   EBGT00050000016458
- HOGENOM:   HBG285270
- OMA:   ADLCFCE
- ProtClustDB:   PRK09283
- BioCyc:   MLEP272631:ML2419-MONOMER
- BRENDA:   4.2.1.24
- InterPro:   IPR001731
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11458
- PIRSF:   PIRSF001415
- PRINTS:   PR00144

Pfam domain/function: PF00490 ALAD

EC number: =4.2.1.24

Molecular weight: Translated: 34775; Mature: 34644

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: ACT_SITE 202-202 ACT_SITE 254-254 BINDING 212-212 BINDING 223-223 BINDING 280-280 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSGYPRHRPRRLRSTPAMRRLVAQTSLEPRNLVLPMFVADGIDELRPIASMPGVVQHT
CCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHHHCCHHHHHH
RDSLRRAAVAAVDAGVGGLNLFGVPRDQDKDATGSAGVDPDGILNVALRDLAEDLGDATV
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEE
LMADTCLDEFTDHGHCGVLDGQGRVDNDATVARYVELAVAQAESGANVVGPSGMMDGQIG
EHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHH
ALRDGLDSAGYADVAILAYAAKFSSAFYGPFREAVSCSLSGDRRTYQQEPGNAREALREI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHHH
KLDLDEGADIIMIKPASGYLDVVATAAGVSPVPVAAYQVSGEYAMICAAAANNWIDERAA
HHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHH
VLESLTSIRRAGADIVFTYWAADVACWLS
HHHHHHHHHHCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure 
SVSGYPRHRPRRLRSTPAMRRLVAQTSLEPRNLVLPMFVADGIDELRPIASMPGVVQHT
CCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHHHCCHHHHHH
RDSLRRAAVAAVDAGVGGLNLFGVPRDQDKDATGSAGVDPDGILNVALRDLAEDLGDATV
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEE
LMADTCLDEFTDHGHCGVLDGQGRVDNDATVARYVELAVAQAESGANVVGPSGMMDGQIG
EHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHH
ALRDGLDSAGYADVAILAYAAKFSSAFYGPFREAVSCSLSGDRRTYQQEPGNAREALREI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHHH
KLDLDEGADIIMIKPASGYLDVVATAAGVSPVPVAAYQVSGEYAMICAAAANNWIDERAA
HHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHH
VLESLTSIRRAGADIVFTYWAADVACWLS
HHHHHHHHHHCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002