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The map label for this gene is hemD [H]

Identifier: 221230779

GI number: 221230779

Start: 2894733

End: 2896424

Strand: Reverse

Name: hemD [H]

Synonym: MLBr_02420

Alternate gene names: 221230779

Gene position: 2896424-2894733 (Counterclockwise)

Preceding gene: 221230780

Following gene: 221230778

Centisome position: 88.63

GC content: 64.01

Gene sequence:

>1692_bases
GTGGTGGGAGCGACAATGACGACGCGAGGGCGTAAGCTTAGACCAGGTCGCATCACATTTGTGGGCGCCGGTCCAGGAGC
CCCAGGGTTACTAACTGCTCAGGCTGTAGCGGTGCTAGCAAACGCCGCCCTTGTTTTTATCGACCCCGATGTACCTGAAC
CGGTGCTGGCGCTGATTGGCAAGGACTTGCCACCGGTCTCTGGTCCGGCGCCTGCCGGTCCGGCTGCCGTGACCGCCGGC
AGGGCTGCTGACGGTACCCTCGAGGGGGATCACTACCAAGCCGCACCGATGGTCATGTCCGGAGGCGCCGACATCCGCCC
TGCGTTGGGTGACCCTACTGAGGTCGCCAAAACGCTGACCGCCGAGGCTCGTTCCGGTGTTGATGTGGTGCGCTTAGTGG
CGGGTGATCCGTTGACGGTGGACGCGGTCATAGCCGAGGTGAACGCTGTGGCACGCACCAATCTACAAATCGAGATCGTG
CCCGGCTTGGTCACCAGCAGCGTCGTACCAACCTATGCCGGGCTACCCTTGGGTTCGTCGCACACGGTGGCTGATGTACG
TGGCGACGTGGACTGGGAAGCGCTAGCCGCGGCTCCTGGGCCGTTGATTCTGCAAGCCACCGCATCACATCTTGCCGACG
CGGCGCACATCTTGATCGACCACGGGCTGGACGACGCCACACCCTGCGTGGTGACCGCTCAGGGCACGACCTGCCAGCAA
CGTTCTGTCGAAACCACATTGGAGGGATTGGCTGACTCGGCTGTCCTGGTCGGCACCGATACCGCAGGCCCGCTGACCGG
GCCGCTGGTGGTGACCATAGGCAAGACGGTGACCAGTCGAGCTAGACTGAACTGGTGGGAAAGCCGTGCACTCTACGGCT
GGACCGTGTTGGTACCGCGTACCAAGGACCAGGCCGGCGAGATGAGCGAGCGGCTGACGTCGTACGGTGCGTTGCCGGTT
GAGGTGCCGACCATCGCTGTCGAGCCGCCGCGCAGTCCCGCTCAGATGGAACGTGCTGTCAAGGGGCTGGTCGACGGTCG
TTTCCAATGGGTGGTGTTCACCTCCACCAACGCGGTGCGCGCGGTGTGGGAGAAGTTTGGCGAGTTCGGTCTGGATGCCC
GCGCCTTCTCCGGGGTGAAGATCGCCTGTGTCGGAGAAGCGACTGCCGACAGGGTCCGTGCATTCGGGATCAGCCCGGAG
CTGGTGCCGGCTGGGGAACAGTCTTCCCTCGGTCTACTGGACGAATTCCCGCCTTATGACAGCGTTTTCGACCCGGTGAA
CCGTGTTTTGCTGCCGCGCGCTGACATTGCCACCGAAACCCTGGCTGAGGGACTGCGCGGGTGTGGCTGGGAGATCGAGG
ACGTCACCGCTTATCGGACGGTGCGGGCGGCACCGCCGCCGGCGGCCACCCGGGAAATGATCAAGACGGGTGGCTTCGAT
GCAGTGTGCTTCACCTCCAGCTCCACGGTGCGTAACTTGGTTGGTATTGCCGGCAAACCGCACGCGCGGACGGTCATTGC
CTGCATCGGACCCAAGACTGCTGAGACCGCTTCCGAATTTGGTTTGCGGGTGGATGTCCAGCCCGAAACCGCCGCGGTTG
GACCGTTAGTTGATGCGTTGGCCGAACATGTCGCTCGACTGCGCGCCGAAGGCGCGCTGCCTCCACCGCGCAAGAAGAGC
CGCAGGCGCTAG

Upstream 100 bases:

>100_bases
CGAGCTTGGGCTAGCAGTTGCCGTGGAGCTGTTTGAGCTGGGTGCCCGGGAGCTGATGTGGGGAGCGCGGTCAGACCGGG
CGCGAGGAAGTTGAACGGGA

Downstream 100 bases:

>100_bases
TAGTGATCGTGAGCACGGTTAGGACGGGCGCGGCAGTAGGCAGTCAGTGAGCGTCAGTGGTTACCCGCGGCACCGGCCGC
GCCGGCTTCGCTCAACCCCC

Product: putative uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MVGATMTTRGRKLRPGRITFVGAGPGAPGLLTAQAVAVLANAALVFIDPDVPEPVLALIGKDLPPVSGPAPAGPAAVTAG
RAADGTLEGDHYQAAPMVMSGGADIRPALGDPTEVAKTLTAEARSGVDVVRLVAGDPLTVDAVIAEVNAVARTNLQIEIV
PGLVTSSVVPTYAGLPLGSSHTVADVRGDVDWEALAAAPGPLILQATASHLADAAHILIDHGLDDATPCVVTAQGTTCQQ
RSVETTLEGLADSAVLVGTDTAGPLTGPLVVTIGKTVTSRARLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGALPV
EVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGEATADRVRAFGISPE
LVPAGEQSSLGLLDEFPPYDSVFDPVNRVLLPRADIATETLAEGLRGCGWEIEDVTAYRTVRAAPPPAATREMIKTGGFD
AVCFTSSSTVRNLVGIAGKPHARTVIACIGPKTAETASEFGLRVDVQPETAAVGPLVDALAEHVARLRAEGALPPPRKKS
RRR

Sequences:

>Translated_563_residues
MVGATMTTRGRKLRPGRITFVGAGPGAPGLLTAQAVAVLANAALVFIDPDVPEPVLALIGKDLPPVSGPAPAGPAAVTAG
RAADGTLEGDHYQAAPMVMSGGADIRPALGDPTEVAKTLTAEARSGVDVVRLVAGDPLTVDAVIAEVNAVARTNLQIEIV
PGLVTSSVVPTYAGLPLGSSHTVADVRGDVDWEALAAAPGPLILQATASHLADAAHILIDHGLDDATPCVVTAQGTTCQQ
RSVETTLEGLADSAVLVGTDTAGPLTGPLVVTIGKTVTSRARLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGALPV
EVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGEATADRVRAFGISPE
LVPAGEQSSLGLLDEFPPYDSVFDPVNRVLLPRADIATETLAEGLRGCGWEIEDVTAYRTVRAAPPPAATREMIKTGGFD
AVCFTSSSTVRNLVGIAGKPHARTVIACIGPKTAETASEFGLRVDVQPETAAVGPLVDALAEHVARLRAEGALPPPRKKS
RRR
>Mature_563_residues
MVGATMTTRGRKLRPGRITFVGAGPGAPGLLTAQAVAVLANAALVFIDPDVPEPVLALIGKDLPPVSGPAPAGPAAVTAG
RAADGTLEGDHYQAAPMVMSGGADIRPALGDPTEVAKTLTAEARSGVDVVRLVAGDPLTVDAVIAEVNAVARTNLQIEIV
PGLVTSSVVPTYAGLPLGSSHTVADVRGDVDWEALAAAPGPLILQATASHLADAAHILIDHGLDDATPCVVTAQGTTCQQ
RSVETTLEGLADSAVLVGTDTAGPLTGPLVVTIGKTVTSRARLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGALPV
EVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGEATADRVRAFGISPE
LVPAGEQSSLGLLDEFPPYDSVFDPVNRVLLPRADIATETLAEGLRGCGWEIEDVTAYRTVRAAPPPAATREMIKTGGFD
AVCFTSSSTVRNLVGIAGKPHARTVIACIGPKTAETASEFGLRVDVQPETAAVGPLVDALAEHVARLRAEGALPPPRKKS
RRR

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=283, Percent_Identity=26.5017667844523, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 58511; Mature: 58511

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGATMTTRGRKLRPGRITFVGAGPGAPGLLTAQAVAVLANAALVFIDPDVPEPVLALIG
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHC
KDLPPVSGPAPAGPAAVTAGRAADGTLEGDHYQAAPMVMSGGADIRPALGDPTEVAKTLT
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCCEEEECCCCCCCCCCCHHHHHHHHH
AEARSGVDVVRLVAGDPLTVDAVIAEVNAVARTNLQIEIVPGLVTSSVVPTYAGLPLGSS
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCHHCCCCCCCC
HTVADVRGDVDWEALAAAPGPLILQATASHLADAAHILIDHGLDDATPCVVTAQGTTCQQ
CCEEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCHHH
RSVETTLEGLADSAVLVGTDTAGPLTGPLVVTIGKTVTSRARLNWWESRALYGWTVLVPR
HHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHCHHHCCCEEEEEEEEEC
TKDQAGEMSERLTSYGALPVEVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVR
CCCHHHHHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHH
AVWEKFGEFGLDARAFSGVKIACVGEATADRVRAFGISPELVPAGEQSSLGLLDEFPPYD
HHHHHHHHCCCCCHHCCCEEEEEECCCCHHHHHEECCCCCEECCCCCCCCCCCCCCCCCC
SVFDPVNRVLLPRADIATETLAEGLRGCGWEIEDVTAYRTVRAAPPPAATREMIKTGGFD
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCC
AVCFTSSSTVRNLVGIAGKPHARTVIACIGPKTAETASEFGLRVDVQPETAAVGPLVDAL
EEEEECCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHH
AEHVARLRAEGALPPPRKKSRRR
HHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MVGATMTTRGRKLRPGRITFVGAGPGAPGLLTAQAVAVLANAALVFIDPDVPEPVLALIG
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHC
KDLPPVSGPAPAGPAAVTAGRAADGTLEGDHYQAAPMVMSGGADIRPALGDPTEVAKTLT
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCCEEEECCCCCCCCCCCHHHHHHHHH
AEARSGVDVVRLVAGDPLTVDAVIAEVNAVARTNLQIEIVPGLVTSSVVPTYAGLPLGSS
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCHHCCCCCCCC
HTVADVRGDVDWEALAAAPGPLILQATASHLADAAHILIDHGLDDATPCVVTAQGTTCQQ
CCEEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCHHH
RSVETTLEGLADSAVLVGTDTAGPLTGPLVVTIGKTVTSRARLNWWESRALYGWTVLVPR
HHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHCHHHCCCEEEEEEEEEC
TKDQAGEMSERLTSYGALPVEVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVR
CCCHHHHHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHH
AVWEKFGEFGLDARAFSGVKIACVGEATADRVRAFGISPELVPAGEQSSLGLLDEFPPYD
HHHHHHHHCCCCCHHCCCEEEEEECCCCHHHHHEECCCCCEECCCCCCCCCCCCCCCCCC
SVFDPVNRVLLPRADIATETLAEGLRGCGWEIEDVTAYRTVRAAPPPAATREMIKTGGFD
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCC
AVCFTSSSTVRNLVGIAGKPHARTVIACIGPKTAETASEFGLRVDVQPETAAVGPLVDAL
EEEEECCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHH
AEHVARLRAEGALPPPRKKSRRR
HHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]