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The map label for this gene is plsB

Identifier: 221230114

GI number: 221230114

Start: 1486049

End: 1488376

Strand: Direct

Name: plsB

Synonym: MLBr_01246

Alternate gene names: 221230114

Gene position: 1486049-1488376 (Clockwise)

Preceding gene: 221230113

Following gene: 221230115

Centisome position: 45.47

GC content: 57.43

Gene sequence:

>2328_bases
GTGACTGAACCGGATGTAGAAATCAGCTCAGTCCTTACCGGTGAAGACACGCTGGTGCTAGCGTCTATGGACACTCCGGC
GGAAATTGAGCTGGTCATGGATTGGCTATGCCAGCAGCGTAACCGCAACCCGGACATCAAGTTCGACGTATTGAAGCTTC
CTTCGCGCAACTTAGCGCCCGCGGCGCTGACAGCACTTGTTGAACAGCTCGAATCCGACGAAGACCGGTCGGTCGTGCCG
GTGCGTGTTTTCTGGATGCCGCCTGCGGAGCGCAGTAAGTTGGCCAAGCTGGCTGGATTGTTGCCCGGCCGGGATCCTTA
CCACCCCAACCGGCGCCAGCAGCGCCACATCTTAAAAACCGACGCCCGGCGTGCCCTGGTGATTGCTGGCGACTCTGCTA
AAGTGTCCGAGCTCCGCCAATACTGGCGCGATACCACCGTTGGAGAAAACGAGTGCGATTTCGCTCAGTTCGTTACTCGC
CGCGCCATCTTGGCGATGGAACGTGCCGAGTCTCGAATCCTCGGACCACAGTACAAGTCTCCGCGGCTGGTGAAGCCAGA
AATCTTGGCGTCAACGCGGTTTCGTGCTGGACTGGAAAAGATCTCGGGCGCAACCGTGGAAGAAGCTGGGAAGATGCTTG
ACGAACTCGCCACCGGGTGGAGCAGGGCGTCGGTTGACCTCGTTTCCGTGCTCGGCAGGATGCTCAGCCGCGGCTTCGAA
CCTGAGATCGACTACGACGAGTATCAAGTCGCGGCGATGCGCGCGGCGTTGGAAGCTCATCCAGCGGTGCTGCTGTTCTC
GCACCGGTCCTACATTGACGGTGCGGTGGTGCCGGTGGCGATGCAGGAGAATCGGCTACCACCGGTGCATGTGTTCGCCG
GCATCAACCTGTCGTTCGGGTTAATGGGGCCACTGTTGCGCCGCTCCGGCGTCATTTTCATCCGCCGTAACATCGGCGAC
AATCCGCTCTACAAGTATGTCTTGCGCGAATACGTCGGCTACATCGTGGAGAAGCGTTTCAACCTGAGCTGGTCCATTGA
GGGCACTCGTTCGCGTACTGGCAAGATGCTGCCACCCAAGCTCGGTCTGCTCACCTACGTGGCCGATGCGTACCTGGACG
GCCGGAGTGAAGACATCCTGTTGCAGCCGGTGTCGATCAGTTTCGATCAGTTGCACGAAACCGCCGAGTACGCTGCCTAT
GCTCGTGGCGGCGAAAAGACGCCCGAAGGTGTCGCTTGGCTGTATAGTTTTATCAAGGCGCAAGGTGAACGTAACTACGG
TAAGATCTACGTCCGTTTCCCGGAAGCGGTCTCGATGCGGCAGTATCTCGGTGCGCCTCACGGTGCATTGGTTCAAGATC
AAGACGCTAAACGGCTTGCGCTCCAAAAGATGTCGTTCGAAGTTGCATGGCGGATTCTGTGTGCGACGCCAGTGACGGCG
ACAGCGTTGGTTTCCGCGCTGCTATTGACCACTCGTGGAGTGGCCTTGACGCTTGATCAACTGCATCACACGTTGCAAGA
ATCACTGGATTACCTGGAACGCAAGCAAACTCCTGTGTCGAAGAGTGCGTTGCGGCTGCGTTCGCGTGAAGGCGTGCGTG
CTGCGGTCGACGCATTGTCCAGCGGGCACCCGATCACTCGGGTTGACAGCGGTCGGGAACCGGTGTGGTATATTACCCCC
GGTAATGAACATGCTGCGGCATTCTACCGGAACTCGGTGATACACGCCTTCCTGGAGACCTCGATAGTCGAACTCGCGTT
GGCGCATGCCAGGCATGTCGAAGGCGACCGTATGAAGGTTTTCTGGGCGCAGGCGATGAGGCTGCGTGATCTCTTGAAGT
TCGATTTTTATTTCGCGGATTCGGCTGCTTTTCGTGCCAATATCGCCGAAGAGATAGCGTGGCACCAGAATTGGGAGGAT
CGTGTTTCCGGTGATGGTGATGATATCGACGCGATGCTGCTTACTAAGCGACCGTTGATCTCAGATGCGATGTTGCGGGT
ATTTTTTGAAGCGTACGATATTGTCGCTGATGTGTTGCGCGATGCTCCGGCGGATGTTGGCCAAAAGGAACTGACTGAAT
TGGCACTTGGTGTCGGACGCCAGTACGTGGCACAGGGTCGGGTCCGTAGCGGTGAATCGGTGTCTACGCTACTATTCGCC
ACCGCTTACCAGGTTGTTGTCGATCAGAATCTGATAGCGCCAGCTCCGGATCTCGCTGAACGTCGGATGGTTTTCCGGCG
GGAGTTGCGGGATATTCGGCGAGATTTCGACTACGTCGAACAAATCGCGCGCAGCCGGTTCATCGTCCGTGAGTTCAAAT
CGCGTTAG

Upstream 100 bases:

>100_bases
GCATGTTGACGCCCCCCACGAGGATATCGGACAAAGAGAACCCGAAAGTACCTAAGGTCGTTTCCCAAGACCTCGTCGTG
AAACAGCCCAGAGAGCGGTC

Downstream 100 bases:

>100_bases
TGACGCTTGTACCGTTCAGTGGCCAAGCTATCGGTCCATACCCAGGGTAGCTGACGGTTGCTCGCTTTGCTGGCAAGCTC
GGGTGTGTCCTTAACTGACT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 775; Mature: 774

Protein sequence:

>775_residues
MTEPDVEISSVLTGEDTLVLASMDTPAEIELVMDWLCQQRNRNPDIKFDVLKLPSRNLAPAALTALVEQLESDEDRSVVP
VRVFWMPPAERSKLAKLAGLLPGRDPYHPNRRQQRHILKTDARRALVIAGDSAKVSELRQYWRDTTVGENECDFAQFVTR
RAILAMERAESRILGPQYKSPRLVKPEILASTRFRAGLEKISGATVEEAGKMLDELATGWSRASVDLVSVLGRMLSRGFE
PEIDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGAVVPVAMQENRLPPVHVFAGINLSFGLMGPLLRRSGVIFIRRNIGD
NPLYKYVLREYVGYIVEKRFNLSWSIEGTRSRTGKMLPPKLGLLTYVADAYLDGRSEDILLQPVSISFDQLHETAEYAAY
ARGGEKTPEGVAWLYSFIKAQGERNYGKIYVRFPEAVSMRQYLGAPHGALVQDQDAKRLALQKMSFEVAWRILCATPVTA
TALVSALLLTTRGVALTLDQLHHTLQESLDYLERKQTPVSKSALRLRSREGVRAAVDALSSGHPITRVDSGREPVWYITP
GNEHAAAFYRNSVIHAFLETSIVELALAHARHVEGDRMKVFWAQAMRLRDLLKFDFYFADSAAFRANIAEEIAWHQNWED
RVSGDGDDIDAMLLTKRPLISDAMLRVFFEAYDIVADVLRDAPADVGQKELTELALGVGRQYVAQGRVRSGESVSTLLFA
TAYQVVVDQNLIAPAPDLAERRMVFRRELRDIRRDFDYVEQIARSRFIVREFKSR

Sequences:

>Translated_775_residues
MTEPDVEISSVLTGEDTLVLASMDTPAEIELVMDWLCQQRNRNPDIKFDVLKLPSRNLAPAALTALVEQLESDEDRSVVP
VRVFWMPPAERSKLAKLAGLLPGRDPYHPNRRQQRHILKTDARRALVIAGDSAKVSELRQYWRDTTVGENECDFAQFVTR
RAILAMERAESRILGPQYKSPRLVKPEILASTRFRAGLEKISGATVEEAGKMLDELATGWSRASVDLVSVLGRMLSRGFE
PEIDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGAVVPVAMQENRLPPVHVFAGINLSFGLMGPLLRRSGVIFIRRNIGD
NPLYKYVLREYVGYIVEKRFNLSWSIEGTRSRTGKMLPPKLGLLTYVADAYLDGRSEDILLQPVSISFDQLHETAEYAAY
ARGGEKTPEGVAWLYSFIKAQGERNYGKIYVRFPEAVSMRQYLGAPHGALVQDQDAKRLALQKMSFEVAWRILCATPVTA
TALVSALLLTTRGVALTLDQLHHTLQESLDYLERKQTPVSKSALRLRSREGVRAAVDALSSGHPITRVDSGREPVWYITP
GNEHAAAFYRNSVIHAFLETSIVELALAHARHVEGDRMKVFWAQAMRLRDLLKFDFYFADSAAFRANIAEEIAWHQNWED
RVSGDGDDIDAMLLTKRPLISDAMLRVFFEAYDIVADVLRDAPADVGQKELTELALGVGRQYVAQGRVRSGESVSTLLFA
TAYQVVVDQNLIAPAPDLAERRMVFRRELRDIRRDFDYVEQIARSRFIVREFKSR
>Mature_774_residues
TEPDVEISSVLTGEDTLVLASMDTPAEIELVMDWLCQQRNRNPDIKFDVLKLPSRNLAPAALTALVEQLESDEDRSVVPV
RVFWMPPAERSKLAKLAGLLPGRDPYHPNRRQQRHILKTDARRALVIAGDSAKVSELRQYWRDTTVGENECDFAQFVTRR
AILAMERAESRILGPQYKSPRLVKPEILASTRFRAGLEKISGATVEEAGKMLDELATGWSRASVDLVSVLGRMLSRGFEP
EIDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGAVVPVAMQENRLPPVHVFAGINLSFGLMGPLLRRSGVIFIRRNIGDN
PLYKYVLREYVGYIVEKRFNLSWSIEGTRSRTGKMLPPKLGLLTYVADAYLDGRSEDILLQPVSISFDQLHETAEYAAYA
RGGEKTPEGVAWLYSFIKAQGERNYGKIYVRFPEAVSMRQYLGAPHGALVQDQDAKRLALQKMSFEVAWRILCATPVTAT
ALVSALLLTTRGVALTLDQLHHTLQESLDYLERKQTPVSKSALRLRSREGVRAAVDALSSGHPITRVDSGREPVWYITPG
NEHAAAFYRNSVIHAFLETSIVELALAHARHVEGDRMKVFWAQAMRLRDLLKFDFYFADSAAFRANIAEEIAWHQNWEDR
VSGDGDDIDAMLLTKRPLISDAMLRVFFEAYDIVADVLRDAPADVGQKELTELALGVGRQYVAQGRVRSGESVSTLLFAT
AYQVVVDQNLIAPAPDLAERRMVFRRELRDIRRDFDYVEQIARSRFIVREFKSR

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=475, Percent_Identity=27.5789473684211, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI190358539, Length=428, Percent_Identity=23.5981308411215, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI87082362, Length=484, Percent_Identity=26.6528925619835, Blast_Score=137, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71988723, Length=516, Percent_Identity=22.6744186046512, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25147672, Length=307, Percent_Identity=27.6872964169381, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988728, Length=428, Percent_Identity=23.3644859813084, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17864692, Length=299, Percent_Identity=27.7591973244147, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24650754, Length=374, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650752, Length=374, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357731, Length=374, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_MYCLB (B8ZRA3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503530.1
- ProteinModelPortal:   B8ZRA3
- EnsemblBacteria:   EBMYCT00000086549
- GeneID:   7327096
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000018019
- HOGENOM:   HBG565163
- OMA:   LEAAFYR
- ProtClustDB:   PRK03355
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 87365; Mature: 87234

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPDVEISSVLTGEDTLVLASMDTPAEIELVMDWLCQQRNRNPDIKFDVLKLPSRNLAP
CCCCCCCHHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCH
AALTALVEQLESDEDRSVVPVRVFWMPPAERSKLAKLAGLLPGRDPYHPNRRQQRHILKT
HHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
DARRALVIAGDSAKVSELRQYWRDTTVGENECDFAQFVTRRAILAMERAESRILGPQYKS
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PRLVKPEILASTRFRAGLEKISGATVEEAGKMLDELATGWSRASVDLVSVLGRMLSRGFE
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
PEIDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGAVVPVAMQENRLPPVHVFAGINLSFG
CCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCHH
LMGPLLRRSGVIFIRRNIGDNPLYKYVLREYVGYIVEKRFNLSWSIEGTRSRTGKMLPPK
HHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCH
LGLLTYVADAYLDGRSEDILLQPVSISFDQLHETAEYAAYARGGEKTPEGVAWLYSFIKA
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QGERNYGKIYVRFPEAVSMRQYLGAPHGALVQDQDAKRLALQKMSFEVAWRILCATPVTA
CCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
TALVSALLLTTRGVALTLDQLHHTLQESLDYLERKQTPVSKSALRLRSREGVRAAVDALS
HHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
SGHPITRVDSGREPVWYITPGNEHAAAFYRNSVIHAFLETSIVELALAHARHVEGDRMKV
CCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
FWAQAMRLRDLLKFDFYFADSAAFRANIAEEIAWHQNWEDRVSGDGDDIDAMLLTKRPLI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEECCCCHH
SDAMLRVFFEAYDIVADVLRDAPADVGQKELTELALGVGRQYVAQGRVRSGESVSTLLFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHH
TAYQVVVDQNLIAPAPDLAERRMVFRRELRDIRRDFDYVEQIARSRFIVREFKSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEPDVEISSVLTGEDTLVLASMDTPAEIELVMDWLCQQRNRNPDIKFDVLKLPSRNLAP
CCCCCCHHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCH
AALTALVEQLESDEDRSVVPVRVFWMPPAERSKLAKLAGLLPGRDPYHPNRRQQRHILKT
HHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
DARRALVIAGDSAKVSELRQYWRDTTVGENECDFAQFVTRRAILAMERAESRILGPQYKS
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PRLVKPEILASTRFRAGLEKISGATVEEAGKMLDELATGWSRASVDLVSVLGRMLSRGFE
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
PEIDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGAVVPVAMQENRLPPVHVFAGINLSFG
CCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCHH
LMGPLLRRSGVIFIRRNIGDNPLYKYVLREYVGYIVEKRFNLSWSIEGTRSRTGKMLPPK
HHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCH
LGLLTYVADAYLDGRSEDILLQPVSISFDQLHETAEYAAYARGGEKTPEGVAWLYSFIKA
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QGERNYGKIYVRFPEAVSMRQYLGAPHGALVQDQDAKRLALQKMSFEVAWRILCATPVTA
CCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
TALVSALLLTTRGVALTLDQLHHTLQESLDYLERKQTPVSKSALRLRSREGVRAAVDALS
HHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
SGHPITRVDSGREPVWYITPGNEHAAAFYRNSVIHAFLETSIVELALAHARHVEGDRMKV
CCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
FWAQAMRLRDLLKFDFYFADSAAFRANIAEEIAWHQNWEDRVSGDGDDIDAMLLTKRPLI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEECCCCHH
SDAMLRVFFEAYDIVADVLRDAPADVGQKELTELALGVGRQYVAQGRVRSGESVSTLLFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHH
TAYQVVVDQNLIAPAPDLAERRMVFRRELRDIRRDFDYVEQIARSRFIVREFKSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA