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The map label for this gene is fadA4

Identifier: 221230062

GI number: 221230062

Start: 1352952

End: 1354133

Strand: Direct

Name: fadA4

Synonym: MLBr_01158

Alternate gene names: 221230062

Gene position: 1352952-1354133 (Clockwise)

Preceding gene: 221230060

Following gene: 221230063

Centisome position: 41.4

GC content: 59.48

Gene sequence:

>1182_bases
ATGACGACATCGGTGATCGTTACTGGAGCACGGACACCCATTGGTAAGTTGATGGGGTCTTTGAAGGACTTTTCGGCTAG
CGATTTGGGCGCGATCACCATTGCCGCTGCGCTGAAGAAAGCCAATGTAGCACCTTCCATTGTGCAGTACGTGATTATAG
GCCAGGTGTTGACCGCGGGGGCCGGTCAGATGCCGGCGCGGCAGGCGGCAGTAGCGGCCGGCATCGGTTGGGATGTGCCG
GCGCTGACGATTAACAAGATGTGTCTGTCTGGTCTTGACGCCATCGCACTCGCTGACCAGCTCATTCGCGCCGGAGAATT
CGATGTGGTGGTGGCTGGCGGCCAAGAGTCCATGACGAAGGCACCACATCTGCTGATGGATAGCCGGTCGGGTTACAAGT
ACGGTGACGTTACCATTGTGGACCATTTAGCCTATGACGGCCTGCACGATGTGTTCACCAATCAGCCGATGGGCGCGCTT
ACCGAACAGCGCAATGATGTCGAAAAATTCACGCGCCAAGAACAGGACGAGTTTGCTGCCCGGTCGCACCAAAAGGCGGC
AGCGGCATGGAAGGACGGCGTCTTCGCCGACGAAGTGGTGCCGGTGAGTATTCCGCAGAGCAAAGGTGATTCGCTGCAGT
TCACTGAGGATGAGGGGATTCGCGCCAACACCAGTGCCGAGTCTTTGGCCGGTCTGAAGCCGGCGTTTCGCTGCGGCGGC
ACTATTACTCCAGGCTCTGCGTCGCAGATCTCCGATGGTGCAGCCACTGTCGTGGTGATGAACAAGGAAAAGGCGCAGCA
GCTGGGGTTGACTTGGCTGGTCGAGATCGGTGCCCACGGCGTTGTAGCCGGCCCAGATTCTACGTTGCAATCCCAGCCGG
CCAACGCAATCAAGAAGGCGGTTGACCGTGAAGGTATCTCCGTCGAGCAGCTCGATGTCGTAGAGATTAACGAGGCGTTC
GCGGCGGTGGCGCTGGCCTCAGCCCGTGAACTTGGTATCGCCCCCGAGCTTGTCAACGTCAACGGCGGAGCGATCGCTGT
CGGTCACCCCCTCGGGATGTCGGGAGCCAGAATAACGTTGCATGTCGCGCTAGAATTGGCGCGTCGCGGTTCGGGTTATG
GTGTTGCGGCTCTGTGCGGTGCCGGTGGCCAGGGTGATGCGCTGATTCTGCGGGCCGTTTAG

Upstream 100 bases:

>100_bases
CGTCATCACACAACGGTAGCCTGACGGGAAAGAGCACGTTTCTCCAGCAGGTTCGAATCGGCCTGCAAAGAGGACCCGAG
AGCAGGGCTAGGGGGTAGTC

Downstream 100 bases:

>100_bases
TTCTGACCTGCGACGCGTCCGGGGTAATATGATTTACGTCACAAAAATGGTTTGCGGCGCCCGAATTCAATTTTCGAACC
GGTTTTGCGACTACGTAAGC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTTSVIVTGARTPIGKLMGSLKDFSASDLGAITIAAALKKANVAPSIVQYVIIGQVLTAGAGQMPARQAAVAAGIGWDVP
ALTINKMCLSGLDAIALADQLIRAGEFDVVVAGGQESMTKAPHLLMDSRSGYKYGDVTIVDHLAYDGLHDVFTNQPMGAL
TEQRNDVEKFTRQEQDEFAARSHQKAAAAWKDGVFADEVVPVSIPQSKGDSLQFTEDEGIRANTSAESLAGLKPAFRCGG
TITPGSASQISDGAATVVVMNKEKAQQLGLTWLVEIGAHGVVAGPDSTLQSQPANAIKKAVDREGISVEQLDVVEINEAF
AAVALASARELGIAPELVNVNGGAIAVGHPLGMSGARITLHVALELARRGSGYGVAALCGAGGQGDALILRAV

Sequences:

>Translated_393_residues
MTTSVIVTGARTPIGKLMGSLKDFSASDLGAITIAAALKKANVAPSIVQYVIIGQVLTAGAGQMPARQAAVAAGIGWDVP
ALTINKMCLSGLDAIALADQLIRAGEFDVVVAGGQESMTKAPHLLMDSRSGYKYGDVTIVDHLAYDGLHDVFTNQPMGAL
TEQRNDVEKFTRQEQDEFAARSHQKAAAAWKDGVFADEVVPVSIPQSKGDSLQFTEDEGIRANTSAESLAGLKPAFRCGG
TITPGSASQISDGAATVVVMNKEKAQQLGLTWLVEIGAHGVVAGPDSTLQSQPANAIKKAVDREGISVEQLDVVEINEAF
AAVALASARELGIAPELVNVNGGAIAVGHPLGMSGARITLHVALELARRGSGYGVAALCGAGGQGDALILRAV
>Mature_392_residues
TTSVIVTGARTPIGKLMGSLKDFSASDLGAITIAAALKKANVAPSIVQYVIIGQVLTAGAGQMPARQAAVAAGIGWDVPA
LTINKMCLSGLDAIALADQLIRAGEFDVVVAGGQESMTKAPHLLMDSRSGYKYGDVTIVDHLAYDGLHDVFTNQPMGALT
EQRNDVEKFTRQEQDEFAARSHQKAAAAWKDGVFADEVVPVSIPQSKGDSLQFTEDEGIRANTSAESLAGLKPAFRCGGT
ITPGSASQISDGAATVVVMNKEKAQQLGLTWLVEIGAHGVVAGPDSTLQSQPANAIKKAVDREGISVEQLDVVEINEAFA
AVALASARELGIAPELVNVNGGAIAVGHPLGMSGARITLHVALELARRGSGYGVAALCGAGGQGDALILRAV

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=45.5012853470437, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI4557237, Length=388, Percent_Identity=38.1443298969072, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI167614485, Length=390, Percent_Identity=39.4871794871795, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=39.3401015228426, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=31.4420803782506, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=28.4615384615385, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=45.1282051282051, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=43.8775510204082, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=38.8059701492537, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=34.3283582089552, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1788683, Length=429, Percent_Identity=30.7692307692308, Blast_Score=168, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=41.3881748071979, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=38.659793814433, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17535921, Length=388, Percent_Identity=38.659793814433, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=34.3511450381679, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17551802, Length=427, Percent_Identity=30.9133489461358, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=393, Percent_Identity=39.440203562341, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=35.8585858585859, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24655093, Length=393, Percent_Identity=43.2569974554707, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24640423, Length=387, Percent_Identity=41.0852713178295, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI17648125, Length=390, Percent_Identity=35.6410256410256, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=31.5914489311164, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_MYCLE (P46707)

Other databases:

- EMBL:   U00014
- EMBL:   AL583921
- PIR:   S72804
- RefSeq:   NP_301848.1
- ProteinModelPortal:   P46707
- SMR:   P46707
- EnsemblBacteria:   EBMYCT00000028884
- GeneID:   910257
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1158
- NMPDR:   fig|272631.1.peg.720
- Leproma:   ML1158
- GeneTree:   EBGT00070000031751
- HOGENOM:   HBG370930
- OMA:   GNVAGPD
- ProtClustDB:   PRK05790
- BioCyc:   MLEP272631:ML1158-MONOMER
- BRENDA:   2.3.1.9
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.9

Molecular weight: Translated: 40530; Mature: 40398

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 88-88 ACT_SITE 349-349 ACT_SITE 379-379

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSVIVTGARTPIGKLMGSLKDFSASDLGAITIAAALKKANVAPSIVQYVIIGQVLTAG
CCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
AGQMPARQAAVAAGIGWDVPALTINKMCLSGLDAIALADQLIRAGEFDVVVAGGQESMTK
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCHHHHH
APHLLMDSRSGYKYGDVTIVDHLAYDGLHDVFTNQPMGALTEQRNDVEKFTRQEQDEFAA
CCHHEEECCCCCEECCEEEEEHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSHQKAAAAWKDGVFADEVVPVSIPQSKGDSLQFTEDEGIRANTSAESLAGLKPAFRCGG
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCCHHHHCCC
TITPGSASQISDGAATVVVMNKEKAQQLGLTWLVEIGAHGVVAGPDSTLQSQPANAIKKA
CCCCCCCCCCCCCCEEEEEECHHHHHHHCCEEEEEECCCEEEECCCCHHHCCCHHHHHHH
VDREGISVEQLDVVEINEAFAAVALASARELGIAPELVNVNGGAIAVGHPLGMSGARITL
HHHCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCCCCEEEE
HVALELARRGSGYGVAALCGAGGQGDALILRAV
EEEHHHHHCCCCCCEEEEECCCCCCCEEEEEEC
>Mature Secondary Structure 
TTSVIVTGARTPIGKLMGSLKDFSASDLGAITIAAALKKANVAPSIVQYVIIGQVLTAG
CCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
AGQMPARQAAVAAGIGWDVPALTINKMCLSGLDAIALADQLIRAGEFDVVVAGGQESMTK
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCHHHHH
APHLLMDSRSGYKYGDVTIVDHLAYDGLHDVFTNQPMGALTEQRNDVEKFTRQEQDEFAA
CCHHEEECCCCCEECCEEEEEHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSHQKAAAAWKDGVFADEVVPVSIPQSKGDSLQFTEDEGIRANTSAESLAGLKPAFRCGG
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCCHHHHCCC
TITPGSASQISDGAATVVVMNKEKAQQLGLTWLVEIGAHGVVAGPDSTLQSQPANAIKKA
CCCCCCCCCCCCCCEEEEEECHHHHHHHCCEEEEEECCCEEEECCCCHHHCCCHHHHHHH
VDREGISVEQLDVVEINEAFAAVALASARELGIAPELVNVNGGAIAVGHPLGMSGARITL
HHHCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCCCCEEEE
HVALELARRGSGYGVAALCGAGGQGDALILRAV
EEEHHHHHCCCCCCEEEEECCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002