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The map label for this gene is hemK

Identifier: 221230043

GI number: 221230043

Start: 1326941

End: 1327807

Strand: Direct

Name: hemK

Synonym: MLBr_01135

Alternate gene names: 221230043

Gene position: 1326941-1327807 (Clockwise)

Preceding gene: 221230042

Following gene: 221230044

Centisome position: 40.6

GC content: 61.71

Gene sequence:

>867_bases
ATGATGATTCGGCTGCGGCGTGCGATTGATTCTGCTGTGACGCAGCTCGAGGAAGCGGGGATCGGTTCTGCGCGTTGCGA
TGCCGAGCAGCTGGCCGCTCACCTGGCTGGCACCGACCGCGGTCGGCTGGCCTTACTTGACACGCCCGGGGAGGAGTTTT
TCAGGCGTTACAGCGACGCCGTTGCCGCGCGGTCGCGGCGGGTGCCGTTGCAGCATCTCATCGGTACGGTGTCGTTCGGG
CCAGTAGTGCTGCATGTCGGCCCGGATGTATTCATACCGCGCCCAGAGACCGAGGCCATATTGGCGTGGGTGATGGCACA
GCGGCTGCCGGAGCGGCCAGTGATTGTGGATGCGTGTACTGGATCCGGCGCTTTAGCGGTCGCGCTGGCTCACCATCGGC
CTGCGGCACGAGTGATTGGCATCGACGATTCCGATTCGGCCCTGGACTACGCACGCCGCAATGCTGAAGGCACCGCAGTA
GAATGCGTGCGTGCCGATGTCACCACCCCGGCTCTGTTGCCTGAACTTGATGGATGCGTCGATCTCTTTGTGGCCAACCC
ACCCTACGTTCCTGACGATCCAGTAGTGCAATCCATTCTGGAACCTGAAGTGACCCAATATGATCCGCGTCACGCGGTAT
TTGGCGGTCCGGACGGAATGGCGCTGACGGCTGACATTGTCGGTCTAGCCGGGCGTTGGCTGCGCCCTGGTGGTTTATTC
GCCGTCGAGCATGACGACAGCACGTCGGTGCCAACGCTTGACTTGGTCTACCGAACAGATCTTTTCGACGATGTCTTGAC
CCACAGAGATCTAGCCGGTCGGCCAAGGTTTGTGACGGCCCGCAGGCGGGAAAGCTGGAGCGCATGA

Upstream 100 bases:

>100_bases
GTTACAAGGCACACAATCTCGATCAGGTCCTAGACGGTGACCTGGATGCGCTTTTTGACGCGTTAAGTGCTGCTGATAAG
CAGTCAAGGTTGCAACAGGT

Downstream 100 bases:

>100_bases
ATTGCGTCGCGACGATGCAGAGCGTAGGGATGAGGAGAAGTGGTACGCATGAATGAGGTGTTCGATTGTGCTGACCCAGA
CCAGCGTGCGCGTGGGATCG

Product: putative protoporphyrinogen oxidase

Products: NA

Alternate protein names: M.MleHemKP

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MMIRLRRAIDSAVTQLEEAGIGSARCDAEQLAAHLAGTDRGRLALLDTPGEEFFRRYSDAVAARSRRVPLQHLIGTVSFG
PVVLHVGPDVFIPRPETEAILAWVMAQRLPERPVIVDACTGSGALAVALAHHRPAARVIGIDDSDSALDYARRNAEGTAV
ECVRADVTTPALLPELDGCVDLFVANPPYVPDDPVVQSILEPEVTQYDPRHAVFGGPDGMALTADIVGLAGRWLRPGGLF
AVEHDDSTSVPTLDLVYRTDLFDDVLTHRDLAGRPRFVTARRRESWSA

Sequences:

>Translated_288_residues
MMIRLRRAIDSAVTQLEEAGIGSARCDAEQLAAHLAGTDRGRLALLDTPGEEFFRRYSDAVAARSRRVPLQHLIGTVSFG
PVVLHVGPDVFIPRPETEAILAWVMAQRLPERPVIVDACTGSGALAVALAHHRPAARVIGIDDSDSALDYARRNAEGTAV
ECVRADVTTPALLPELDGCVDLFVANPPYVPDDPVVQSILEPEVTQYDPRHAVFGGPDGMALTADIVGLAGRWLRPGGLF
AVEHDDSTSVPTLDLVYRTDLFDDVLTHRDLAGRPRFVTARRRESWSA
>Mature_288_residues
MMIRLRRAIDSAVTQLEEAGIGSARCDAEQLAAHLAGTDRGRLALLDTPGEEFFRRYSDAVAARSRRVPLQHLIGTVSFG
PVVLHVGPDVFIPRPETEAILAWVMAQRLPERPVIVDACTGSGALAVALAHHRPAARVIGIDDSDSALDYARRNAEGTAV
ECVRADVTTPALLPELDGCVDLFVANPPYVPDDPVVQSILEPEVTQYDPRHAVFGGPDGMALTADIVGLAGRWLRPGGLF
AVEHDDSTSVPTLDLVYRTDLFDDVLTHRDLAGRPRFVTARRRESWSA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=286, Percent_Identity=32.1678321678322, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1787463, Length=241, Percent_Identity=34.0248962655602, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87082085, Length=188, Percent_Identity=31.9148936170213, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582226, Length=284, Percent_Identity=30.2816901408451, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_MYCLE (P45832)

Other databases:

- EMBL:   U15186
- EMBL:   AL583920
- PIR:   A87051
- RefSeq:   NP_301829.1
- ProteinModelPortal:   P45832
- SMR:   P45832
- EnsemblBacteria:   EBMYCT00000029112
- GeneID:   910229
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1135
- NMPDR:   fig|272631.1.peg.701
- Leproma:   ML1135
- GeneTree:   EBGT00050000016869
- HOGENOM:   HBG732041
- OMA:   NRIPLGH
- ProtClustDB:   PRK09328
- BioCyc:   MLEP272631:ML1135-MONOMER
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 31226; Mature: 31226

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00092 N6_MTASE; PS00338 SOMATOTROPIN_2

Important sites: BINDING 142-142 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIRLRRAIDSAVTQLEEAGIGSARCDAEQLAAHLAGTDRGRLALLDTPGEEFFRRYSDA
CEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHH
VAARSRRVPLQHLIGTVSFGPVVLHVGPDVFIPRPETEAILAWVMAQRLPERPVIVDACT
HHHHHCCCCHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHCCCCCEEEEECC
GSGALAVALAHHRPAARVIGIDDSDSALDYARRNAEGTAVECVRADVTTPALLPELDGCV
CCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCE
DLFVANPPYVPDDPVVQSILEPEVTQYDPRHAVFGGPDGMALTADIVGLAGRWLRPGGLF
EEEECCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCEE
AVEHDDSTSVPTLDLVYRTDLFDDVLTHRDLAGRPRFVTARRRESWSA
EEECCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCC
>Mature Secondary Structure
MMIRLRRAIDSAVTQLEEAGIGSARCDAEQLAAHLAGTDRGRLALLDTPGEEFFRRYSDA
CEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHH
VAARSRRVPLQHLIGTVSFGPVVLHVGPDVFIPRPETEAILAWVMAQRLPERPVIVDACT
HHHHHCCCCHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHCCCCCEEEEECC
GSGALAVALAHHRPAARVIGIDDSDSALDYARRNAEGTAVECVRADVTTPALLPELDGCV
CCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCE
DLFVANPPYVPDDPVVQSILEPEVTQYDPRHAVFGGPDGMALTADIVGLAGRWLRPGGLF
EEEECCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCEE
AVEHDDSTSVPTLDLVYRTDLFDDVLTHRDLAGRPRFVTARRRESWSA
EEECCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002