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The map label for this gene is prfA

Identifier: 221230042

GI number: 221230042

Start: 1325859

End: 1326944

Strand: Direct

Name: prfA

Synonym: MLBr_01134

Alternate gene names: 221230042

Gene position: 1325859-1326944 (Clockwise)

Preceding gene: 221230041

Following gene: 221230043

Centisome position: 40.57

GC content: 59.21

Gene sequence:

>1086_bases
ATGGCGCAGCCGGTGCAATCGATCGACGTGTTGCTTATCGAACACGCTGAACTTGAGCTTGCATTGGCAGACCCGGAACT
GCACAGCAATCCCGCCGAGGCGCGCAAAGCCGGACGTCGGTTCGCCCGATTGGCCCCGATCGTGGCCACGCACCGCAAGC
TGATATCGGCGCGCGACGATTTGCAGACCGCACGTGAACTCGCCGCCGGCGATGAGTCGTTCGCTGACGAGATCGCCGAA
CTGGAATCACGGATAGCCGAACTAACTACCCAACTCACTGACATGCTGGCGCCACATGACCCGCATGGCCCCGACGACAT
AGTGCTTGAAGTGAAATCCGGCGAGGGTGGTGAAGAGTCGGCCTTGTTCGCTGCTGACCTTGCGAGGATGTACATCCGCT
ACGCCGAACGGCACGGTTGGACGGTGACGGTGCTGGACGAAACCACCTCGGATCTGGGCGGCTATAAGGACGCGACGTTA
GCGATTTCTCATAAGGGTGCCAGTGATGGTGACGCTGTCGACGGGGTGTGGTCGCGAATGAAGTTCGAAGGCGGGGTGCA
CCGGGTGCAACGGGTACCGGTAACGGAATCCCAAGGCCGTGTGCATACTTCGGCAGCGGGCGTGCTGGTCTATCCGGAAC
CCGAGGAAATAGGTGAAGTGCACATCGACGAGTCGGATCTGCGCATCGATGTCTACCGGTCTTCAGGCAAAGGCGGCCAA
GGCGTTAATACAACCGATTCCGCGGTGCGGATCACTCATCTACCTACCGGGGTTGTTGTCACCTGTCAAAACGAACGGTC
GCAGCTGCAGAACAAGACACGTGCGTTGCAGGTGTTGGCCGCCCGCCTGCAGGCAATGGCCGAGGAGCAAGCACTAGCCA
ACGCGTCGGCGGATCGGGCCAGCCAGATTCGCACGGTGGACCGTAGTGAACGCATTCGCACCTACAACTTCCCGGAGAAC
CGGATCACGGATCACCGAATCGGTTACAAGGCACACAATCTCGATCAGGTCCTAGACGGTGACCTGGATGCGCTTTTTGA
CGCGTTAAGTGCTGCTGATAAGCAGTCAAGGTTGCAACAGGTATGA

Upstream 100 bases:

>100_bases
CAATAAATAGCTGGCTCACTGCCGTCCGAACTGTGCGTGAACCTGCACAGGCCGGGTGTCGTTTCGTGTTTGGTGGTAGC
AGTTTGCGGTAGGAGTTGAT

Downstream 100 bases:

>100_bases
TGATTCGGCTGCGGCGTGCGATTGATTCTGCTGTGACGCAGCTCGAGGAAGCGGGGATCGGTTCTGCGCGTTGCGATGCC
GAGCAGCTGGCCGCTCACCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAQPVQSIDVLLIEHAELELALADPELHSNPAEARKAGRRFARLAPIVATHRKLISARDDLQTARELAAGDESFADEIAE
LESRIAELTTQLTDMLAPHDPHGPDDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDETTSDLGGYKDATL
AISHKGASDGDAVDGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEIGEVHIDESDLRIDVYRSSGKGGQ
GVNTTDSAVRITHLPTGVVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALANASADRASQIRTVDRSERIRTYNFPEN
RITDHRIGYKAHNLDQVLDGDLDALFDALSAADKQSRLQQV

Sequences:

>Translated_361_residues
MAQPVQSIDVLLIEHAELELALADPELHSNPAEARKAGRRFARLAPIVATHRKLISARDDLQTARELAAGDESFADEIAE
LESRIAELTTQLTDMLAPHDPHGPDDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDETTSDLGGYKDATL
AISHKGASDGDAVDGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEIGEVHIDESDLRIDVYRSSGKGGQ
GVNTTDSAVRITHLPTGVVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALANASADRASQIRTVDRSERIRTYNFPEN
RITDHRIGYKAHNLDQVLDGDLDALFDALSAADKQSRLQQV
>Mature_360_residues
AQPVQSIDVLLIEHAELELALADPELHSNPAEARKAGRRFARLAPIVATHRKLISARDDLQTARELAAGDESFADEIAEL
ESRIAELTTQLTDMLAPHDPHGPDDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDETTSDLGGYKDATLA
ISHKGASDGDAVDGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEIGEVHIDESDLRIDVYRSSGKGGQG
VNTTDSAVRITHLPTGVVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALANASADRASQIRTVDRSERIRTYNFPENR
ITDHRIGYKAHNLDQVLDGDLDALFDALSAADKQSRLQQV

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=47.5352112676056, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=37.5, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI166795305, Length=181, Percent_Identity=47.5138121546961, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1787462, Length=350, Percent_Identity=44.5714285714286, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=35.7771260997067, Blast_Score=197, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=280, Percent_Identity=36.7857142857143, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321295, Length=376, Percent_Identity=36.436170212766, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI19921226, Length=273, Percent_Identity=42.8571428571429, Blast_Score=211, Evalue=7e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_MYCLB (B8ZR31)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503458.1
- ProteinModelPortal:   B8ZR31
- SMR:   B8ZR31
- EnsemblBacteria:   EBMYCT00000087019
- GeneID:   7326668
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015865
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39492; Mature: 39361

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPVQSIDVLLIEHAELELALADPELHSNPAEARKAGRRFARLAPIVATHRKLISARDD
CCCCHHHHHEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTARELAAGDESFADEIAELESRIAELTTQLTDMLAPHDPHGPDDIVLEVKSGEGGEES
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWTVTVLDETTSDLGGYKDATLAISHKGASDGDAVDGVWSRM
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHH
KFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEIGEVHIDESDLRIDVYRSSGKGGQ
HHCCCHHHHHCCCCCCCCCCEEECCCEEEEECCHHHHCEEEECCCCCEEEEEECCCCCCC
GVNTTDSAVRITHLPTGVVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALANASADRA
CCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQIRTVDRSERIRTYNFPENRITDHRIGYKAHNLDQVLDGDLDALFDALSAADKQSRLQQ
HHHHHCCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHC
V
C
>Mature Secondary Structure 
AQPVQSIDVLLIEHAELELALADPELHSNPAEARKAGRRFARLAPIVATHRKLISARDD
CCCHHHHHEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTARELAAGDESFADEIAELESRIAELTTQLTDMLAPHDPHGPDDIVLEVKSGEGGEES
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWTVTVLDETTSDLGGYKDATLAISHKGASDGDAVDGVWSRM
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHH
KFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEIGEVHIDESDLRIDVYRSSGKGGQ
HHCCCHHHHHCCCCCCCCCCEEECCCEEEEECCHHHHCEEEECCCCCEEEEEECCCCCCC
GVNTTDSAVRITHLPTGVVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALANASADRA
CCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQIRTVDRSERIRTYNFPENRITDHRIGYKAHNLDQVLDGDLDALFDALSAADKQSRLQQ
HHHHHCCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA