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The map label for this gene is rho [H]

Identifier: 221230040

GI number: 221230040

Start: 1323371

End: 1325203

Strand: Direct

Name: rho [H]

Synonym: MLBr_01132

Alternate gene names: 221230040

Gene position: 1323371-1325203 (Clockwise)

Preceding gene: 221230039

Following gene: 221230041

Centisome position: 40.49

GC content: 59.9

Gene sequence:

>1833_bases
GTGACTGAAACGGACCTTATCACGGTTGGCGAAAACACTGATGACACAGAGCTGACGAACGCCGTGACTACAGACACTCC
CGATGTCAAAGCCACTGCGGCCACCGTGTCACTGGGATCGCTGTCCACGATGGTGCTGCCTGAACTGCGGGCGCTGGCTA
ATCAAGCCGGTGTTAAGGGCACGTCGGGCATGCGTAAGAGCGAACTAATCGCCGCAATTGAGGAATGCAGGGGGCAAGCC
AACGGCACGTCGGTCAACGATGGCCCATCGCGAGACCACGGTGGATCTGCAACAGCCATCAGCACCGAAGCACTGGCTGC
CCAAGAGGAACAGAACTATGCGATAGTCGAGGTGTCCCGCCGGGAACGACGCGGCGCTTCTCGTGAAGCGGATGTCACGG
CCGGCACTAGCACAGCAGAGGCCACGGAGTCGGATTGTCAGGGCACTGCTGATGATGACACTCGCACCCTCCAAGGGGGC
CAATCGGACACCAAGACCGAAGAGCGCGGACCAGATGTGGGTAACGATCAGGGTGTTGAACAGCAGAGTTCGAGTTTGCA
GCCGCGCGGTGACGACGACGGCGAGGGCCGCCAGGGCAGGCGCGGACGCCGGTTTCGTGATCGCGATCGCAGGCGGCGCG
GTGAGCGGTCCGGTGACGGTGCCGAGGCCGAATTGCGTGAGGACGACGTCGTCCAGCCGGTCGCCGGTATCCTTGATGTT
CTCGACAATTACGCGTTCGTTCGTACCTCTGGTTATCTGGCTGGTCCGCACGATGTCTATGTGTCTATGAGTATGGTGCG
CAAGAACGGCTTGCGTCGGGGTGACGCAGTGACCGGAGCTGTTCGGGTGCCCCGAGAAGGTGAGCAGGGCCACCAGCGCC
AGAAGTTCAACCCGCTGGTGCGTCTGGACAGTATTAACGGTGGATCGGTTGAAGATGCTAAGAAGCGGCCCGAGTTCGGC
AAACTGACACCGTTGTACCCCAATCAGCGGCTTCGTCTGGAAACCACGCCCGATCGGTTGACCACCAGAGTTATAGACTT
GATCATGCCGATCGGCAAGGGCCAACGCGCGTTGATCGTGTCGCCGCCCAAGGCTGGTAAGACCACGATCCTGCAGGACA
TCGCTAACGCGATCACCAGGAACAATCTGGAATGTCATCTGATGGTCGTGCTGGTCGACGAGCGACCTGAAGAGGTCACC
GACATGCAGCGTTCAGTCAAGGGCGAGGTCATCGCTTCGACATTCGATCGGCCGCCGTCGGATCACACTTCGGTCGCCGA
GCTGGCTATTGAACGCGCCAAGCGGCTCGTCGAGCAGGGCAAAGACGTCGTCGTCTTGCTCGACTCGATCACTCGCCTGG
GGCGCGCCTATAACAACGCGTCGCCGGCCTCGGGTCGCATCCTCTCCGGTGGTGTCGATTCCACCGCGCTTTACCCGCCT
AAGCGATTCCTCGGCGCCGCTCGCAACATCGAGGAAGGTGGGTCGCTGACCATCATTGCCACCGCGATGGTCGAGACCGG
GTCCACCGGAGACATGGTTATCTTTGAGGAGTTCAAAGGTACCGGTAACGCTGAACTGAAGCTGGACCGCAAGATTGCTG
AGCGACGGGTTTTCCCCGCTGTGGACGTCAACCCCTCCGGTACTCGCAAAGACGAGTTGTTGCTGTCGCCTGACGAGTTC
GGCATCGTGCACAAGCTGCGCCGTGTGTTGTCGGGTCTGGATTCCCACCAGGCCATCGACTTGTTGATGTCGCAGTTACG
CAAGACCAAGACAAATTACGAGTTCCTGGTGCAGGTATCCAAGACTACGCCAGGTTCCATGGACGATGATTAG

Upstream 100 bases:

>100_bases
ATCACCGTTAATTAGAGTCGATGATTGGGCGCGCTCAGCACTTTGGCTGAATGCAACGACTCCTCGCATGACGTGATCAG
CGAGGGAAGAAAGGAAATCC

Downstream 100 bases:

>100_bases
CGGCCTAAGCCGCTGGTGAAGTACGGCGAGCAATGGCGCGACACAGCCACTACGTACGCGACACGACCTGACGGGCGTCT
CCTTGGCGGCTACTTGGTGA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTETDLITVGENTDDTELTNAVTTDTPDVKATAATVSLGSLSTMVLPELRALANQAGVKGTSGMRKSELIAAIEECRGQA
NGTSVNDGPSRDHGGSATAISTEALAAQEEQNYAIVEVSRRERRGASREADVTAGTSTAEATESDCQGTADDDTRTLQGG
QSDTKTEERGPDVGNDQGVEQQSSSLQPRGDDDGEGRQGRRGRRFRDRDRRRRGERSGDGAEAELREDDVVQPVAGILDV
LDNYAFVRTSGYLAGPHDVYVSMSMVRKNGLRRGDAVTGAVRVPREGEQGHQRQKFNPLVRLDSINGGSVEDAKKRPEFG
KLTPLYPNQRLRLETTPDRLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANAITRNNLECHLMVVLVDERPEEVT
DMQRSVKGEVIASTFDRPPSDHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPP
KRFLGAARNIEEGGSLTIIATAMVETGSTGDMVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKDELLLSPDEF
GIVHKLRRVLSGLDSHQAIDLLMSQLRKTKTNYEFLVQVSKTTPGSMDDD

Sequences:

>Translated_610_residues
MTETDLITVGENTDDTELTNAVTTDTPDVKATAATVSLGSLSTMVLPELRALANQAGVKGTSGMRKSELIAAIEECRGQA
NGTSVNDGPSRDHGGSATAISTEALAAQEEQNYAIVEVSRRERRGASREADVTAGTSTAEATESDCQGTADDDTRTLQGG
QSDTKTEERGPDVGNDQGVEQQSSSLQPRGDDDGEGRQGRRGRRFRDRDRRRRGERSGDGAEAELREDDVVQPVAGILDV
LDNYAFVRTSGYLAGPHDVYVSMSMVRKNGLRRGDAVTGAVRVPREGEQGHQRQKFNPLVRLDSINGGSVEDAKKRPEFG
KLTPLYPNQRLRLETTPDRLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANAITRNNLECHLMVVLVDERPEEVT
DMQRSVKGEVIASTFDRPPSDHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPP
KRFLGAARNIEEGGSLTIIATAMVETGSTGDMVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKDELLLSPDEF
GIVHKLRRVLSGLDSHQAIDLLMSQLRKTKTNYEFLVQVSKTTPGSMDDD
>Mature_609_residues
TETDLITVGENTDDTELTNAVTTDTPDVKATAATVSLGSLSTMVLPELRALANQAGVKGTSGMRKSELIAAIEECRGQAN
GTSVNDGPSRDHGGSATAISTEALAAQEEQNYAIVEVSRRERRGASREADVTAGTSTAEATESDCQGTADDDTRTLQGGQ
SDTKTEERGPDVGNDQGVEQQSSSLQPRGDDDGEGRQGRRGRRFRDRDRRRRGERSGDGAEAELREDDVVQPVAGILDVL
DNYAFVRTSGYLAGPHDVYVSMSMVRKNGLRRGDAVTGAVRVPREGEQGHQRQKFNPLVRLDSINGGSVEDAKKRPEFGK
LTPLYPNQRLRLETTPDRLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANAITRNNLECHLMVVLVDERPEEVTD
MQRSVKGEVIASTFDRPPSDHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPK
RFLGAARNIEEGGSLTIIATAMVETGSTGDMVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKDELLLSPDEFG
IVHKLRRVLSGLDSHQAIDLLMSQLRKTKTNYEFLVQVSKTTPGSMDDD

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=409, Percent_Identity=52.078239608802, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1788251, Length=264, Percent_Identity=26.1363636363636, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 66105; Mature: 65973

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETDLITVGENTDDTELTNAVTTDTPDVKATAATVSLGSLSTMVLPELRALANQAGVKG
CCCCCEEEECCCCCCHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
TSGMRKSELIAAIEECRGQANGTSVNDGPSRDHGGSATAISTEALAAQEEQNYAIVEVSR
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEEEEECH
RERRGASREADVTAGTSTAEATESDCQGTADDDTRTLQGGQSDTKTEERGPDVGNDQGVE
HHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCHHHCCCCCCCCCCCH
QQSSSLQPRGDDDGEGRQGRRGRRFRDRDRRRRGERSGDGAEAELREDDVVQPVAGILDV
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LDNYAFVRTSGYLAGPHDVYVSMSMVRKNGLRRGDAVTGAVRVPREGEQGHQRQKFNPLV
HHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHCCCEE
RLDSINGGSVEDAKKRPEFGKLTPLYPNQRLRLETTPDRLTTRVIDLIMPIGKGQRALIV
EEECCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEE
SPPKAGKTTILQDIANAITRNNLECHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRPPS
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCC
DHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPP
CCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
KRFLGAARNIEEGGSLTIIATAMVETGSTGDMVIFEEFKGTGNAELKLDRKIAERRVFPA
HHHHHHHCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEHHHHHHHHCCCC
VDVNPSGTRKDELLLSPDEFGIVHKLRRVLSGLDSHQAIDLLMSQLRKTKTNYEFLVQVS
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEE
KTTPGSMDDD
CCCCCCCCCC
>Mature Secondary Structure 
TETDLITVGENTDDTELTNAVTTDTPDVKATAATVSLGSLSTMVLPELRALANQAGVKG
CCCCEEEECCCCCCHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
TSGMRKSELIAAIEECRGQANGTSVNDGPSRDHGGSATAISTEALAAQEEQNYAIVEVSR
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEEEEECH
RERRGASREADVTAGTSTAEATESDCQGTADDDTRTLQGGQSDTKTEERGPDVGNDQGVE
HHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCHHHCCCCCCCCCCCH
QQSSSLQPRGDDDGEGRQGRRGRRFRDRDRRRRGERSGDGAEAELREDDVVQPVAGILDV
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LDNYAFVRTSGYLAGPHDVYVSMSMVRKNGLRRGDAVTGAVRVPREGEQGHQRQKFNPLV
HHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHCCCEE
RLDSINGGSVEDAKKRPEFGKLTPLYPNQRLRLETTPDRLTTRVIDLIMPIGKGQRALIV
EEECCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEE
SPPKAGKTTILQDIANAITRNNLECHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRPPS
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCC
DHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPP
CCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
KRFLGAARNIEEGGSLTIIATAMVETGSTGDMVIFEEFKGTGNAELKLDRKIAERRVFPA
HHHHHHHCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEHHHHHHHHCCCC
VDVNPSGTRKDELLLSPDEFGIVHKLRRVLSGLDSHQAIDLLMSQLRKTKTNYEFLVQVS
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEE
KTTPGSMDDD
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]