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The map label for this gene is thrB

Identifier: 221230039

GI number: 221230039

Start: 1322154

End: 1323101

Strand: Direct

Name: thrB

Synonym: MLBr_01131

Alternate gene names: 221230039

Gene position: 1322154-1323101 (Clockwise)

Preceding gene: 221230038

Following gene: 221230040

Centisome position: 40.46

GC content: 61.39

Gene sequence:

>948_bases
TTGGTGACCTGGATGCTGCCTGCTGGTCTGGTGGCCAGCGCCGTGGTGGCGGCGTCTAGCGCTAACCTCGGTCCGGGTTT
CGATAGCATCGGTCTGGCATTGAGTCTTTGTGACGAGATTGTGGTCGAGACAACAGATTCCGGCTTGGTTGTTGTCGTCG
ATGGCGAGGGAGCCGACCAGGTGCCGATGGGCCCTGAACATCTGGTAGTGCGTGCCGTCCGACGCGGCCTGCAGGCTGTT
GGGGTGAGTGCGGCTGGCCTGGTAGTGCGCTGCCGCAACGCTATCCCCCACTCCCGTGGTCTCGGCTCCTCCGCGGCGGC
TGTGGTCGGTGGCCTAGCAGTGGTGAACGGCTTTGTTGCCCAGATTGATTCGACACCGCTAAGCAACGCTCAGCTAATCC
AGCTTGCGTCGGAGTTCGAGGGTCATCCCGACAACGCGGCGGCTGCGGTGTTGGGCGGTGCGGTGGTTTCGTGGGTTGAC
CGCAGTTATGATCAGCCTGACTACTGCGCGGTACCGCTGCGGCTTCATCCCGATATCCATCTCTTCGCCGCTATTCCCGA
GGAACGTTCTTCAACCGCGGAGAGTCGGGTGCTGTTGCCTGCCCGGGTTAGCCACGACGACGCGAGATTCAATGTGAGTC
GTGCCGCGTTGTTGGTGGTAGCACTTACTGAACGGCCTGATCTTCTGATGGCCGCGACAGAAGACGTGCTTCACCAGCCG
CACCGCGCCTCGGCGATGTCGGCTTCGGCGGAATATTTGCGACTGTTGCGGCGTCATAACGTGGCAGCGACGCTTTCTGG
AGCGGGTCCATCCCTAATTGCCTTGAGCACACAGTCGGAGTTGCCCCGGGAAGCGGCAGAGTACGGCGCCGCAAACGGAT
TTATCATCATCAAGATGACCGCCGGGGATGAAGTGTGCTGGCGGCCAGAGGTCACAGTTCCCGGTTAA

Upstream 100 bases:

>100_bases
GTCGCTGTTGTCGAGCAGTTAGGGCTGGTCTAGTGGTGATCGCAAGTGCGACGCAGTTGGGTGCGGTGGGGCGCCACTCG
TTGGGCTCAGTGAAAGGCTG

Downstream 100 bases:

>100_bases
TTCACACCGTTGCCGGCGGGTTTGCTTGCTTCCGGCAAGGAAGCGGGCTATCCTTGAACCTGTCCAGCAATTGCAGCATC
TGCATGTGCATTCATACTGT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MVTWMLPAGLVASAVVAASSANLGPGFDSIGLALSLCDEIVVETTDSGLVVVVDGEGADQVPMGPEHLVVRAVRRGLQAV
GVSAAGLVVRCRNAIPHSRGLGSSAAAVVGGLAVVNGFVAQIDSTPLSNAQLIQLASEFEGHPDNAAAAVLGGAVVSWVD
RSYDQPDYCAVPLRLHPDIHLFAAIPEERSSTAESRVLLPARVSHDDARFNVSRAALLVVALTERPDLLMAATEDVLHQP
HRASAMSASAEYLRLLRRHNVAATLSGAGPSLIALSTQSELPREAAEYGAANGFIIIKMTAGDEVCWRPEVTVPG

Sequences:

>Translated_315_residues
MVTWMLPAGLVASAVVAASSANLGPGFDSIGLALSLCDEIVVETTDSGLVVVVDGEGADQVPMGPEHLVVRAVRRGLQAV
GVSAAGLVVRCRNAIPHSRGLGSSAAAVVGGLAVVNGFVAQIDSTPLSNAQLIQLASEFEGHPDNAAAAVLGGAVVSWVD
RSYDQPDYCAVPLRLHPDIHLFAAIPEERSSTAESRVLLPARVSHDDARFNVSRAALLVVALTERPDLLMAATEDVLHQP
HRASAMSASAEYLRLLRRHNVAATLSGAGPSLIALSTQSELPREAAEYGAANGFIIIKMTAGDEVCWRPEVTVPG
>Mature_315_residues
MVTWMLPAGLVASAVVAASSANLGPGFDSIGLALSLCDEIVVETTDSGLVVVVDGEGADQVPMGPEHLVVRAVRRGLQAV
GVSAAGLVVRCRNAIPHSRGLGSSAAAVVGGLAVVNGFVAQIDSTPLSNAQLIQLASEFEGHPDNAAAAVLGGAVVSWVD
RSYDQPDYCAVPLRLHPDIHLFAAIPEERSSTAESRVLLPARVSHDDARFNVSRAALLVVALTERPDLLMAATEDVLHQP
HRASAMSASAEYLRLLRRHNVAATLSGAGPSLIALSTQSELPREAAEYGAANGFIIIKMTAGDEVCWRPEVTVPG

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=301, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321814, Length=334, Percent_Identity=32.0359281437126, Blast_Score=122, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_MYCLB (B8ZR28)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503455.1
- ProteinModelPortal:   B8ZR28
- SMR:   B8ZR28
- EnsemblBacteria:   EBMYCT00000086635
- GeneID:   7326020
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016145
- HOGENOM:   HBG646290
- OMA:   FDCLGAA
- ProtClustDB:   PRK01212
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 32753; Mature: 32753

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTWMLPAGLVASAVVAASSANLGPGFDSIGLALSLCDEIVVETTDSGLVVVVDGEGADQ
CEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCC
VPMGPEHLVVRAVRRGLQAVGVSAAGLVVRCRNAIPHSRGLGSSAAAVVGGLAVVNGFVA
CCCCHHHHHHHHHHHHHHHHCCCHHHHHEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
QIDSTPLSNAQLIQLASEFEGHPDNAAAAVLGGAVVSWVDRSYDQPDYCAVPLRLHPDIH
HHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEE
LFAAIPEERSSTAESRVLLPARVSHDDARFNVSRAALLVVALTERPDLLMAATEDVLHQP
EEEECCCHHCCCCHHEEEEEEECCCCCCCEEHHHEEEEEEEECCCCCEEEEECHHHHCCC
HRASAMSASAEYLRLLRRHNVAATLSGAGPSLIALSTQSELPREAAEYGAANGFIIIKMT
CCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECHHHCHHHHHHHCCCCCEEEEEEC
AGDEVCWRPEVTVPG
CCCCEEECCCCCCCC
>Mature Secondary Structure
MVTWMLPAGLVASAVVAASSANLGPGFDSIGLALSLCDEIVVETTDSGLVVVVDGEGADQ
CEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCC
VPMGPEHLVVRAVRRGLQAVGVSAAGLVVRCRNAIPHSRGLGSSAAAVVGGLAVVNGFVA
CCCCHHHHHHHHHHHHHHHHCCCHHHHHEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
QIDSTPLSNAQLIQLASEFEGHPDNAAAAVLGGAVVSWVDRSYDQPDYCAVPLRLHPDIH
HHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEE
LFAAIPEERSSTAESRVLLPARVSHDDARFNVSRAALLVVALTERPDLLMAATEDVLHQP
EEEECCCHHCCCCHHEEEEEEECCCCCCCEEHHHEEEEEEEECCCCCEEEEECHHHHCCC
HRASAMSASAEYLRLLRRHNVAATLSGAGPSLIALSTQSELPREAAEYGAANGFIIIKMT
CCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECHHHCHHHHHHHCCCCCEEEEEEC
AGDEVCWRPEVTVPG
CCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA