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The map label for this gene is lysA

Identifier: 221230036

GI number: 221230036

Start: 1318260

End: 1319678

Strand: Direct

Name: lysA

Synonym: MLBr_01128

Alternate gene names: 221230036

Gene position: 1318260-1319678 (Clockwise)

Preceding gene: 221230035

Following gene: 221230037

Centisome position: 40.34

GC content: 58.56

Gene sequence:

>1419_bases
GTGAACGTACATACCGCCGGTCCTCGGCACGCTGAAAAAACCCGTCACACCGCGACTCCACAGCGTGTGCAACCCTCAGA
TGACCTGTTGCGATTAGCGTCAAATGTCTGGCCGCGTAACATTACTCGAGATGAAACCGGAGTTGCTTGCATTGCGGGAA
ATAAACTGACCGACCTTGCCGGGGAGTATGGGACTCCGTTGTTTGTCATCGACGAAGACGACTTCCGTTTCCGTTGCCGG
GAGATAGCGGCTGCATTCGGCGGTGGGGAAAATGTGCACTATGCCGCCAAGGCCTTCTTGTGTACCGAGATAGCTCGGTG
GATCGACGAAGAAGGTCTTTCTCTCGACGTGTGCAGCGGCGGTGAGTTGGCCGTTGCGCTGCATGCTTCTTTTCCACCAG
AGCGTATTAGCTTGCACGGTAACAACAAATCGGTAGCAGAGTTGAAAGATGCAGTCAAAGCCGGAGTGGGCTATATCGTT
TTAGACTCGACGACCGAAATCGAGCGCCTCGATGCCATCGCGGGCGAAGCGGGCATCGTCCAAGATGTCTTGGTGCGCCT
GACTGTCGGTGTAGAGGCGCACACTCATGAGTTCATCGCCACTGCACATGAAGATCAAAAATTCGGGCTGTCGGTTGCCA
GCGGTGCGGCGATGGCTGCGGTCCGCCGGGTATTCGCAACCGATAATCTGCGACTGGTTGGGCTGCACAGCCACATCGGT
TCGCAGATCTTCGATGTCGCCGGCTTCGAACTTGCTGCACACCGTGTCATCGGCCTACTGTGCGATATTGTCGGCGAGTT
TGATCCGGAGAAGACAGCCCAGCTATCGATCGTCGACCTCGGTGGTGGCCTGGGGATCTCGTACTTGCCGGATGACGACC
CACCGCCGATATTCGAGCTGGCGGCCAAGCTGGGCGCTATCGTGAGCAACGAGTCAGCCGCCGTGGGGCTGCCGGTGCCT
AAGTTGATGGTCGAACCCGGACGCGCCATCGCCGGACCGGGCACCATCACGTTGTATGAGGTCGGCACTATTAAGGACGT
CGACGTGAGTGCTACCGCGCACCGGCGCTACGTCAGCATCGATGGGGGGATGAGCGACAACATCCGCACAGCGCTTTACG
ACGCGCAATATGACGTCCGACTTGTCTCGAGAACTAGCGATGCGCCAGCGGCACCTGCCAGCATCGTCGGAAAGCATTGC
GAAAGTGGCGATATCGTTGTACGTGACACCTGGGTTCCTGACGACTTGAAACCCGGCGACCTGGTTGGGGTGGCCGCAAC
CGGGGCCTACTGCTATTCGTTGTCAAGTCGTTACAACATGCTCGGCCGCCCCGCTGTGGTTGCGGTGTGCGCGGGGCAAG
CTCGTCTGATCCTGCGCCGGGAGACGGTTGACGATCTGCTGAGTCTGGAAGTGAGGTGA

Upstream 100 bases:

>100_bases
CTACCGACCTGCACACCGCGCGTTTGGCCCTGTGCCAGGCCAATCGTCAGGTTATTGCCAACGGGCTGGCGATCCTCGGA
GTCAGTGCTCCGGAGCGGAT

Downstream 100 bases:

>100_bases
CCTGTGTTCAGTGATGAAAGGACGGTCGGTGTAGCGGTACTCGGGTTGGGCAATGTCGGCAGCGAAGTTGTTCGCATTAT
CGAGGGCAGCGCTGACGACC

Product: diaminopimelate decarboxylase

Products: NA

Alternate protein names: DAP decarboxylase

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNVHTAGPRHAEKTRHTATPQRVQPSDDLLRLASNVWPRNITRDETGVACIAGNKLTDLAGEYGTPLFVIDEDDFRFRCR
EIAAAFGGGENVHYAAKAFLCTEIARWIDEEGLSLDVCSGGELAVALHASFPPERISLHGNNKSVAELKDAVKAGVGYIV
LDSTTEIERLDAIAGEAGIVQDVLVRLTVGVEAHTHEFIATAHEDQKFGLSVASGAAMAAVRRVFATDNLRLVGLHSHIG
SQIFDVAGFELAAHRVIGLLCDIVGEFDPEKTAQLSIVDLGGGLGISYLPDDDPPPIFELAAKLGAIVSNESAAVGLPVP
KLMVEPGRAIAGPGTITLYEVGTIKDVDVSATAHRRYVSIDGGMSDNIRTALYDAQYDVRLVSRTSDAPAAPASIVGKHC
ESGDIVVRDTWVPDDLKPGDLVGVAATGAYCYSLSSRYNMLGRPAVVAVCAGQARLILRRETVDDLLSLEVR

Sequences:

>Translated_472_residues
MNVHTAGPRHAEKTRHTATPQRVQPSDDLLRLASNVWPRNITRDETGVACIAGNKLTDLAGEYGTPLFVIDEDDFRFRCR
EIAAAFGGGENVHYAAKAFLCTEIARWIDEEGLSLDVCSGGELAVALHASFPPERISLHGNNKSVAELKDAVKAGVGYIV
LDSTTEIERLDAIAGEAGIVQDVLVRLTVGVEAHTHEFIATAHEDQKFGLSVASGAAMAAVRRVFATDNLRLVGLHSHIG
SQIFDVAGFELAAHRVIGLLCDIVGEFDPEKTAQLSIVDLGGGLGISYLPDDDPPPIFELAAKLGAIVSNESAAVGLPVP
KLMVEPGRAIAGPGTITLYEVGTIKDVDVSATAHRRYVSIDGGMSDNIRTALYDAQYDVRLVSRTSDAPAAPASIVGKHC
ESGDIVVRDTWVPDDLKPGDLVGVAATGAYCYSLSSRYNMLGRPAVVAVCAGQARLILRRETVDDLLSLEVR
>Mature_472_residues
MNVHTAGPRHAEKTRHTATPQRVQPSDDLLRLASNVWPRNITRDETGVACIAGNKLTDLAGEYGTPLFVIDEDDFRFRCR
EIAAAFGGGENVHYAAKAFLCTEIARWIDEEGLSLDVCSGGELAVALHASFPPERISLHGNNKSVAELKDAVKAGVGYIV
LDSTTEIERLDAIAGEAGIVQDVLVRLTVGVEAHTHEFIATAHEDQKFGLSVASGAAMAAVRRVFATDNLRLVGLHSHIG
SQIFDVAGFELAAHRVIGLLCDIVGEFDPEKTAQLSIVDLGGGLGISYLPDDDPPPIFELAAKLGAIVSNESAAVGLPVP
KLMVEPGRAIAGPGTITLYEVGTIKDVDVSATAHRRYVSIDGGMSDNIRTALYDAQYDVRLVSRTSDAPAAPASIVGKHC
ESGDIVVRDTWVPDDLKPGDLVGVAATGAYCYSLSSRYNMLGRPAVVAVCAGQARLILRRETVDDLLSLEVR

Specific function: Lysine biosynthesis; last step. [C]

COG id: COG0019

COG function: function code E; Diaminopimelate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family

Homologues:

Organism=Escherichia coli, GI1789203, Length=435, Percent_Identity=28.0459770114943, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCDA_MYCLE (Q50140)

Other databases:

- EMBL:   U15186
- EMBL:   AL583920
- PIR:   B87050
- RefSeq:   NP_301822.1
- ProteinModelPortal:   Q50140
- SMR:   Q50140
- EnsemblBacteria:   EBMYCT00000028706
- GeneID:   910222
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1128
- NMPDR:   fig|272631.1.peg.694
- Leproma:   ML1128
- GeneTree:   EBGT00050000017053
- HOGENOM:   HBG672375
- OMA:   HYASKAF
- ProtClustDB:   CLSK791086
- BioCyc:   MLEP272631:ML1128-MONOMER
- BRENDA:   4.1.1.20
- InterPro:   IPR009006
- InterPro:   IPR002986
- InterPro:   IPR022643
- InterPro:   IPR022644
- InterPro:   IPR022653
- InterPro:   IPR000183
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR01181
- PRINTS:   PR01179
- TIGRFAMs:   TIGR01048

Pfam domain/function: PF02784 Orn_Arg_deC_N; PF00278 Orn_DAP_Arg_deC; SSF50621 Racem_decarbox_C

EC number: =4.1.1.20

Molecular weight: Translated: 50278; Mature: 50278

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00878 ODR_DC_2_1; PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVHTAGPRHAEKTRHTATPQRVQPSDDLLRLASNVWPRNITRDETGVACIAGNKLTDLA
CCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
GEYGTPLFVIDEDDFRFRCREIAAAFGGGENVHYAAKAFLCTEIARWIDEEGLSLDVCSG
HCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
GELAVALHASFPPERISLHGNNKSVAELKDAVKAGVGYIVLDSTTEIERLDAIAGEAGIV
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCHH
QDVLVRLTVGVEAHTHEFIATAHEDQKFGLSVASGAAMAAVRRVFATDNLRLVGLHSHIG
HHHHHHHHCCCCHHCHHHHEECCCCCCCCEEHHCCHHHHHHHHHHHCCCEEEEEEHHHHC
SQIFDVAGFELAAHRVIGLLCDIVGEFDPEKTAQLSIVDLGGGLGISYLPDDDPPPIFEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
AAKLGAIVSNESAAVGLPVPKLMVEPGRAIAGPGTITLYEVGTIKDVDVSATAHRRYVSI
HHHHHHEECCCCCEECCCCCHHEECCCCEECCCCEEEEEEECCEEECCCCCCCCEEEEEE
DGGMSDNIRTALYDAQYDVRLVSRTSDAPAAPASIVGKHCESGDIVVRDTWVPDDLKPGD
CCCCCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCC
LVGVAATGAYCYSLSSRYNMLGRPAVVAVCAGQARLILRRETVDDLLSLEVR
EEEEEECCCEEEEHHHHHCCCCCCEEEEEECCCEEEEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MNVHTAGPRHAEKTRHTATPQRVQPSDDLLRLASNVWPRNITRDETGVACIAGNKLTDLA
CCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
GEYGTPLFVIDEDDFRFRCREIAAAFGGGENVHYAAKAFLCTEIARWIDEEGLSLDVCSG
HCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
GELAVALHASFPPERISLHGNNKSVAELKDAVKAGVGYIVLDSTTEIERLDAIAGEAGIV
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCHH
QDVLVRLTVGVEAHTHEFIATAHEDQKFGLSVASGAAMAAVRRVFATDNLRLVGLHSHIG
HHHHHHHHCCCCHHCHHHHEECCCCCCCCEEHHCCHHHHHHHHHHHCCCEEEEEEHHHHC
SQIFDVAGFELAAHRVIGLLCDIVGEFDPEKTAQLSIVDLGGGLGISYLPDDDPPPIFEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
AAKLGAIVSNESAAVGLPVPKLMVEPGRAIAGPGTITLYEVGTIKDVDVSATAHRRYVSI
HHHHHHEECCCCCEECCCCCHHEECCCCEECCCCEEEEEEECCEEECCCCCCCCEEEEEE
DGGMSDNIRTALYDAQYDVRLVSRTSDAPAAPASIVGKHCESGDIVVRDTWVPDDLKPGD
CCCCCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCC
LVGVAATGAYCYSLSSRYNMLGRPAVVAVCAGQARLILRRETVDDLLSLEVR
EEEEEECCCEEEEHHHHHCCCCCCEEEEEECCCEEEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002