Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 221229951

GI number: 221229951

Start: 1163355

End: 1164290

Strand: Direct

Name: miaA

Synonym: MLBr_00995

Alternate gene names: 221229951

Gene position: 1163355-1164290 (Clockwise)

Preceding gene: 221229950

Following gene: 221229952

Centisome position: 35.6

GC content: 62.61

Gene sequence:

>936_bases
ATGAGGCCACTTGCGATTGTCGGTCCGACGGGTGTTGGCAAGTCGGAACTGGCCCTCGACGTCATCGAGCGACTTGGCGG
CCAGGTTAGCGTGGAGATCGTCAATGCTGATGCGATGCAGCTCTATCGCGGTATGGACATTGGCACAGCGAAGTTGCCCG
TCGCGGCACGTCGAGGGATCCCGCACCATCAACTTGACGTCCTGGACGTCACCGAGACTGCGACGGTTGCCAGCTATCAG
CGGACTGCTGCCGCCGATATCGAGGCGATCGCGGCACGTGGAGCGGTGCCGGTCGTGGTGGGTGGTTCGATGCTCTATGT
CCAGTCGCTGCTCGACGACTGGTCGTTTCCGGGGACCGATCCGGCCGTGCGCGTGAGGTGGGAGCAGCAACTTGCCGAGG
TCGGAGTGGTTAGGCTGCACGCGGAACTGGCCCGTCGTGACCTGGCAGCAGCTGCGGCAATCCTGCCTACCGATGGCCGG
CGCACTGTGCGGGCACTTGAGGTGGTCGAACTCACTGGAAAGCCCTTCGCCGCGTCCGCGCCGCGCATCGGTGCTCCTCG
GTGGGACACTGTGATCGTCGGGTTAGATTGTGACAGAACGATTCTCAGTGAGAGATTGGCGCGGCGCATTGACTCGATGT
TCGGGCAAGGTCTGGTAGATGAGGTACGCATGCTGTTACGCTGGGGCCTGCGCGACGGGGTCACTGCCTCACGTGCGCTG
GGATACGCTCAAGTGCTCACGGCACTTGACGCTGGAGGGGATGCCGACCATTTGGATGAGGCGCGTCAGCAGACATACTT
GGGTCATCGACGCTATGCGCGACGGCAGCGGTCGTGGTTTCATCGAGATCACCGAGTGCACTGGCTCGACGTGGGTACAG
TCGATCGCGTTGGGGTCGTCGACGACGCTTTACGGGTGTGGCGTAACGCATCCTGA

Upstream 100 bases:

>100_bases
AGGTGTTGGCCGGTTTGGTCGAGCCAGGGCCTAGATTGGCGAAGGAGTTATACCGGGGAGCACCCTACGGTGTGGGATAT
TTCGTTGGTGTCTGGCAGCC

Downstream 100 bases:

>100_bases
TCTAGGTTATCCGTTTATCCGTGCCGGCGACGATGCAGAGCGCAGCGATGAGGTGGAGCGGCGTACATGATCTTCGCCAA
GGGGCACGGCACCCAAAATG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRPLAIVGPTGVGKSELALDVIERLGGQVSVEIVNADAMQLYRGMDIGTAKLPVAARRGIPHHQLDVLDVTETATVASYQ
RTAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPGTDPAVRVRWEQQLAEVGVVRLHAELARRDLAAAAAILPTDGR
RTVRALEVVELTGKPFAASAPRIGAPRWDTVIVGLDCDRTILSERLARRIDSMFGQGLVDEVRMLLRWGLRDGVTASRAL
GYAQVLTALDAGGDADHLDEARQQTYLGHRRYARRQRSWFHRDHRVHWLDVGTVDRVGVVDDALRVWRNAS

Sequences:

>Translated_311_residues
MRPLAIVGPTGVGKSELALDVIERLGGQVSVEIVNADAMQLYRGMDIGTAKLPVAARRGIPHHQLDVLDVTETATVASYQ
RTAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPGTDPAVRVRWEQQLAEVGVVRLHAELARRDLAAAAAILPTDGR
RTVRALEVVELTGKPFAASAPRIGAPRWDTVIVGLDCDRTILSERLARRIDSMFGQGLVDEVRMLLRWGLRDGVTASRAL
GYAQVLTALDAGGDADHLDEARQQTYLGHRRYARRQRSWFHRDHRVHWLDVGTVDRVGVVDDALRVWRNAS
>Mature_311_residues
MRPLAIVGPTGVGKSELALDVIERLGGQVSVEIVNADAMQLYRGMDIGTAKLPVAARRGIPHHQLDVLDVTETATVASYQ
RTAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPGTDPAVRVRWEQQLAEVGVVRLHAELARRDLAAAAAILPTDGR
RTVRALEVVELTGKPFAASAPRIGAPRWDTVIVGLDCDRTILSERLARRIDSMFGQGLVDEVRMLLRWGLRDGVTASRAL
GYAQVLTALDAGGDADHLDEARQQTYLGHRRYARRQRSWFHRDHRVHWLDVGTVDRVGVVDDALRVWRNAS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=32.0512820512821, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI1790613, Length=293, Percent_Identity=35.4948805460751, Blast_Score=154, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI25144712, Length=241, Percent_Identity=28.2157676348548, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=28.4246575342466, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24649830, Length=242, Percent_Identity=34.297520661157, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_MYCLB (B8ZQU0)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503367.1
- ProteinModelPortal:   B8ZQU0
- SMR:   B8ZQU0
- EnsemblBacteria:   EBMYCT00000086761
- GeneID:   7326726
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016412
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34054; Mature: 34054

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLAIVGPTGVGKSELALDVIERLGGQVSVEIVNADAMQLYRGMDIGTAKLPVAARRGI
CCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCCCCCCCHHHHCCC
PHHQLDVLDVTETATVASYQRTAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPGTD
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCC
PAVRVRWEQQLAEVGVVRLHAELARRDLAAAAAILPTDGRRTVRALEVVELTGKPFAASA
HHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
PRIGAPRWDTVIVGLDCDRTILSERLARRIDSMFGQGLVDEVRMLLRWGLRDGVTASRAL
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
GYAQVLTALDAGGDADHLDEARQQTYLGHRRYARRQRSWFHRDHRVHWLDVGTVDRVGVV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHH
DDALRVWRNAS
HHHHHHHCCCC
>Mature Secondary Structure
MRPLAIVGPTGVGKSELALDVIERLGGQVSVEIVNADAMQLYRGMDIGTAKLPVAARRGI
CCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCCCCCCCHHHHCCC
PHHQLDVLDVTETATVASYQRTAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPGTD
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCC
PAVRVRWEQQLAEVGVVRLHAELARRDLAAAAAILPTDGRRTVRALEVVELTGKPFAASA
HHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
PRIGAPRWDTVIVGLDCDRTILSERLARRIDSMFGQGLVDEVRMLLRWGLRDGVTASRAL
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
GYAQVLTALDAGGDADHLDEARQQTYLGHRRYARRQRSWFHRDHRVHWLDVGTVDRVGVV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHH
DDALRVWRNAS
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA