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The map label for this gene is dapF

Identifier: 221229952

GI number: 221229952

Start: 1164357

End: 1165247

Strand: Direct

Name: dapF

Synonym: MLBr_00996

Alternate gene names: 221229952

Gene position: 1164357-1165247 (Clockwise)

Preceding gene: 221229951

Following gene: 221229953

Centisome position: 35.63

GC content: 63.3

Gene sequence:

>891_bases
ATGATCTTCGCCAAGGGGCACGGCACCCAAAATGACTTTGTGGTGTTGCCTGATGTCGAGGCTGATGTGACGTTCACCGC
TGCCCAGGTCGCTGCATTGTGCAACCGGCGCCAAGGACTGGGCGCTGACGGGGTGCTCCGGGTGACTACCGCGGGCGCAG
CAGTGACGGCCGGTGTGCTCGAGCACCTGCCTGACGGTGTCAGCTGCTCCGACTGGTACATGGATTACCGCAACGCCGAC
GGGTCGGTGGCGCAGATGTGTGGCAACGGTGTGCGGGTGTTCGCGCATTACCTGCGCGCCAGCGGCTTGGAGTCTTGCGA
TGAGTTCGTCGTTGGCTCATTGGCTGGCCCGCGACTGGTTAACGTGCACCATGTCGACGAGCTCAACGCCGACGTCACAG
TCGACATGGGCAAGGCAAACTTGCTGGGCAGTGGGGGGCCGGCCTTTGCGGTTACTGTCGGCGGGAGGCGATTTTCCGGC
GTAGCGGTAGACGTGGGTAACCCACACCTGGCGTGCATGGATCCGCAGCTGAGTTTGGAAGAGCTCGCAGCTCTAGACTT
GGGTGCGCCGGTCCACCTCGACCGTGTGCAGTTTCCAGACGGGGTCAATATCGAAGTGCTCACCGCACCGGTCGACGGAA
TGGTCCAGATGCGAGTCCACGAGCGCGGGGTGGGTGAAACTCGGTCATGCGGTACCGGAACTGTCGCGGCCGCGGTCGCT
GCGCTGGCAAGTGCTGGCGCCGACACCGGGACGCTTACTGTGAGGGTGCCGGGTGGCGACGTTGTCATAACTATCACTGA
CGTGACCAGCTACTTGCGTGGACCATCGGTGTTAGTGGCGCACGGCGAGCTCGCGGACGCCTGGTGGTACTCGTTGGCGC
GCAGCTGTTAA

Upstream 100 bases:

>100_bases
CGACGCTTTACGGGTGTGGCGTAACGCATCCTGATCTAGGTTATCCGTTTATCCGTGCCGGCGACGATGCAGAGCGCAGC
GATGAGGTGGAGCGGCGTAC

Downstream 100 bases:

>100_bases
ATGTTGCGGGAATTTATTCGGATGCGCGCCCAGCTACGAAGTGCCACCATCGGATACTGGCTATGAAATATCCAGATTCG
TCCGATAGGGGCTCTGCAGA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIFAKGHGTQNDFVVLPDVEADVTFTAAQVAALCNRRQGLGADGVLRVTTAGAAVTAGVLEHLPDGVSCSDWYMDYRNAD
GSVAQMCGNGVRVFAHYLRASGLESCDEFVVGSLAGPRLVNVHHVDELNADVTVDMGKANLLGSGGPAFAVTVGGRRFSG
VAVDVGNPHLACMDPQLSLEELAALDLGAPVHLDRVQFPDGVNIEVLTAPVDGMVQMRVHERGVGETRSCGTGTVAAAVA
ALASAGADTGTLTVRVPGGDVVITITDVTSYLRGPSVLVAHGELADAWWYSLARSC

Sequences:

>Translated_296_residues
MIFAKGHGTQNDFVVLPDVEADVTFTAAQVAALCNRRQGLGADGVLRVTTAGAAVTAGVLEHLPDGVSCSDWYMDYRNAD
GSVAQMCGNGVRVFAHYLRASGLESCDEFVVGSLAGPRLVNVHHVDELNADVTVDMGKANLLGSGGPAFAVTVGGRRFSG
VAVDVGNPHLACMDPQLSLEELAALDLGAPVHLDRVQFPDGVNIEVLTAPVDGMVQMRVHERGVGETRSCGTGTVAAAVA
ALASAGADTGTLTVRVPGGDVVITITDVTSYLRGPSVLVAHGELADAWWYSLARSC
>Mature_296_residues
MIFAKGHGTQNDFVVLPDVEADVTFTAAQVAALCNRRQGLGADGVLRVTTAGAAVTAGVLEHLPDGVSCSDWYMDYRNAD
GSVAQMCGNGVRVFAHYLRASGLESCDEFVVGSLAGPRLVNVHHVDELNADVTVDMGKANLLGSGGPAFAVTVGGRRFSG
VAVDVGNPHLACMDPQLSLEELAALDLGAPVHLDRVQFPDGVNIEVLTAPVDGMVQMRVHERGVGETRSCGTGTVAAAVA
ALASAGADTGTLTVRVPGGDVVITITDVTSYLRGPSVLVAHGELADAWWYSLARSC

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=293, Percent_Identity=31.3993174061433, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_MYCLB (B8ZQU1)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503368.1
- ProteinModelPortal:   B8ZQU1
- SMR:   B8ZQU1
- EnsemblBacteria:   EBMYCT00000087341
- GeneID:   7324901
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017180
- HOGENOM:   HBG399442
- OMA:   NACGTAM
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30640; Mature: 30640

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 87-87 ACT_SITE 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFAKGHGTQNDFVVLPDVEADVTFTAAQVAALCNRRQGLGADGVLRVTTAGAAVTAGVL
CEEECCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
EHLPDGVSCSDWYMDYRNADGSVAQMCGNGVRVFAHYLRASGLESCDEFVVGSLAGPRLV
HHCCCCCCHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEE
NVHHVDELNADVTVDMGKANLLGSGGPAFAVTVGGRRFSGVAVDVGNPHLACMDPQLSLE
EEEEHHHCCCCEEEECCCCEEECCCCCEEEEEECCCEECCEEEECCCCEEEEECCCCCHH
ELAALDLGAPVHLDRVQFPDGVNIEVLTAPVDGMVQMRVHERGVGETRSCGTGTVAAAVA
HHHHHCCCCCEEECEEECCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCCCCHHHHHHHH
ALASAGADTGTLTVRVPGGDVVITITDVTSYLRGPSVLVAHGELADAWWYSLARSC
HHHHCCCCCCEEEEEECCCCEEEEEECHHHHHCCCEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIFAKGHGTQNDFVVLPDVEADVTFTAAQVAALCNRRQGLGADGVLRVTTAGAAVTAGVL
CEEECCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
EHLPDGVSCSDWYMDYRNADGSVAQMCGNGVRVFAHYLRASGLESCDEFVVGSLAGPRLV
HHCCCCCCHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEE
NVHHVDELNADVTVDMGKANLLGSGGPAFAVTVGGRRFSGVAVDVGNPHLACMDPQLSLE
EEEEHHHCCCCEEEECCCCEEECCCCCEEEEEECCCEECCEEEECCCCEEEEECCCCCHH
ELAALDLGAPVHLDRVQFPDGVNIEVLTAPVDGMVQMRVHERGVGETRSCGTGTVAAAVA
HHHHHCCCCCEEECEEECCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCCCCHHHHHHHH
ALASAGADTGTLTVRVPGGDVVITITDVTSYLRGPSVLVAHGELADAWWYSLARSC
HHHHCCCCCCEEEEEECCCCEEEEEECHHHHHCCCEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA