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The map label for this gene is rbsB [H]

Identifier: 221229601

GI number: 221229601

Start: 496069

End: 497106

Strand: Reverse

Name: rbsB [H]

Synonym: MLBr_00398

Alternate gene names: 221229601

Gene position: 497106-496069 (Counterclockwise)

Preceding gene: 221229607

Following gene: 221229600

Centisome position: 15.21

GC content: 56.74

Gene sequence:

>1038_bases
ATGAGCACAGGAACGAGTGTACCACTGCGCGTGGCTAGCATTATGCTGATCCTGGGACTGCTACCAGCAATCCTGCCGGC
GTGTGGTTTCTCAGAGTCACATCACAAGCTGACCATCGGCGTGTCCTATCCTACCGCGAACAGTCCGTTCTGGAATGCCT
ACACACGCTTTATCGACGAGGGAAGCCACCAACTCGGTGTCTATATCAATGCCGTGTCGGCTGAAGAAGACGAACAAAAA
CAACTGTCCGACGTTGAGACCCTCATCAACCAAGGCATCGACGGGCTTATCGTCACCCCCCAGTCAACATCGATCGCTCC
CACGCTGCTGCGGATAGCCACCCAGGCAAACATCCCGGTGGTCGTAGTCGACCGCTACCCAGGATATGCCCCGGGGCAGA
ACAAAAACGCTGATTATGTGGCCTTCCTGGGACCCAATAATGAGAAAGCCGGAAGCGGCATTGCTGAAGCACTCATGGCT
GGCGGCGGGTCGAAATTCCTTGCCCTCGGTGGCATGCCCGGCAACTCGGTGGCCCAGGGCCGCAAGGCTGGCCTGGAATC
AGCGCTCACGGCAGTCGGTCACCGGCTTGTGCAGTTTCAGTACGCCGGTGACAGCGAAGACAAAGGCCTTGCCGCCGCCG
AGAACATGCTGCAAGCACACCCCGCCGGCGACGCCAACGCCATCTGGTGCTTCAACGACAAACTCTGCCAGGGCGCAATC
AAAGCAGTATACAACGCCAATCGTGAAAAGGAATTCATCTTTGGAGGAATGGATTTGACACCGCAAGCAATCGCAGCGAT
CGAAAACGGATTGTACACCGTCAGCTTGGGCGCCCATTGGCTAGAGGGCGGGTTCGGGCTGGCAATCCTCTATCGCAAAA
TCCACGGCCAAGATCCTGCTGAGCGGATGGTGAAGCTCGACCTGCTCAAGGTTGACAAATCCAACGTCGCCAAGTTCAAG
GCCCGCTACATCGACAACACGCCACACTACAACTGGAAGTCCACAACCCCGTTCGATATGACGATCACCTTGAACTGA

Upstream 100 bases:

>100_bases
TACCGCACAACAATTGATTCAACTCATGTCAGACGGCGTAAGACCAAACTGTCGATGCGCCTGACCCAGCGTCACTGCCG
GCCCCACGAAAGGACTCCTG

Downstream 100 bases:

>100_bases
TCAAAAAATGTCCGATCTGTTCCAGACAACCCGTACTAGGCAACTCCTATGGTGCTTTGTAAGCGCTTAACTGGACTCAG
AACACCGTCGCCGCAGTTGA

Product: putative D-ribose-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSTGTSVPLRVASIMLILGLLPAILPACGFSESHHKLTIGVSYPTANSPFWNAYTRFIDEGSHQLGVYINAVSAEEDEQK
QLSDVETLINQGIDGLIVTPQSTSIAPTLLRIATQANIPVVVVDRYPGYAPGQNKNADYVAFLGPNNEKAGSGIAEALMA
GGGSKFLALGGMPGNSVAQGRKAGLESALTAVGHRLVQFQYAGDSEDKGLAAAENMLQAHPAGDANAIWCFNDKLCQGAI
KAVYNANREKEFIFGGMDLTPQAIAAIENGLYTVSLGAHWLEGGFGLAILYRKIHGQDPAERMVKLDLLKVDKSNVAKFK
ARYIDNTPHYNWKSTTPFDMTITLN

Sequences:

>Translated_345_residues
MSTGTSVPLRVASIMLILGLLPAILPACGFSESHHKLTIGVSYPTANSPFWNAYTRFIDEGSHQLGVYINAVSAEEDEQK
QLSDVETLINQGIDGLIVTPQSTSIAPTLLRIATQANIPVVVVDRYPGYAPGQNKNADYVAFLGPNNEKAGSGIAEALMA
GGGSKFLALGGMPGNSVAQGRKAGLESALTAVGHRLVQFQYAGDSEDKGLAAAENMLQAHPAGDANAIWCFNDKLCQGAI
KAVYNANREKEFIFGGMDLTPQAIAAIENGLYTVSLGAHWLEGGFGLAILYRKIHGQDPAERMVKLDLLKVDKSNVAKFK
ARYIDNTPHYNWKSTTPFDMTITLN
>Mature_344_residues
STGTSVPLRVASIMLILGLLPAILPACGFSESHHKLTIGVSYPTANSPFWNAYTRFIDEGSHQLGVYINAVSAEEDEQKQ
LSDVETLINQGIDGLIVTPQSTSIAPTLLRIATQANIPVVVVDRYPGYAPGQNKNADYVAFLGPNNEKAGSGIAEALMAG
GGSKFLALGGMPGNSVAQGRKAGLESALTAVGHRLVQFQYAGDSEDKGLAAAENMLQAHPAGDANAIWCFNDKLCQGAIK
AVYNANREKEFIFGGMDLTPQAIAAIENGLYTVSLGAHWLEGGFGLAILYRKIHGQDPAERMVKLDLLKVDKSNVAKFKA
RYIDNTPHYNWKSTTPFDMTITLN

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=250, Percent_Identity=28.4, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790526, Length=279, Percent_Identity=26.8817204301075, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790674, Length=247, Percent_Identity=23.8866396761134, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36940; Mature: 36809

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGTSVPLRVASIMLILGLLPAILPACGFSESHHKLTIGVSYPTANSPFWNAYTRFIDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHC
GSHQLGVYINAVSAEEDEQKQLSDVETLINQGIDGLIVTPQSTSIAPTLLRIATQANIPV
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCE
VVVDRYPGYAPGQNKNADYVAFLGPNNEKAGSGIAEALMAGGGSKFLALGGMPGNSVAQG
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHH
RKAGLESALTAVGHRLVQFQYAGDSEDKGLAAAENMLQAHPAGDANAIWCFNDKLCQGAI
HHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHH
KAVYNANREKEFIFGGMDLTPQAIAAIENGLYTVSLGAHWLEGGFGLAILYRKIHGQDPA
HHHHCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEECCHHHCCCCHHHHHHHHHCCCCHH
ERMVKLDLLKVDKSNVAKFKARYIDNTPHYNWKSTTPFDMTITLN
HHHHHHHEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
STGTSVPLRVASIMLILGLLPAILPACGFSESHHKLTIGVSYPTANSPFWNAYTRFIDE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHC
GSHQLGVYINAVSAEEDEQKQLSDVETLINQGIDGLIVTPQSTSIAPTLLRIATQANIPV
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCE
VVVDRYPGYAPGQNKNADYVAFLGPNNEKAGSGIAEALMAGGGSKFLALGGMPGNSVAQG
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHH
RKAGLESALTAVGHRLVQFQYAGDSEDKGLAAAENMLQAHPAGDANAIWCFNDKLCQGAI
HHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHH
KAVYNANREKEFIFGGMDLTPQAIAAIENGLYTVSLGAHWLEGGFGLAILYRKIHGQDPA
HHHHCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEECCHHHCCCCHHHHHHHHHCCCCHH
ERMVKLDLLKVDKSNVAKFKARYIDNTPHYNWKSTTPFDMTITLN
HHHHHHHEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]