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The map label for this gene is phsB [H]

Identifier: 220918377

GI number: 220918377

Start: 3649677

End: 3650498

Strand: Direct

Name: phsB [H]

Synonym: A2cp1_3281

Alternate gene names: 220918377

Gene position: 3649677-3650498 (Clockwise)

Preceding gene: 220918376

Following gene: 220918378

Centisome position: 72.57

GC content: 74.57

Gene sequence:

>822_bases
ATGGACGACCGCACGCCGTGCCCCGCCTCGACCGGAGGCCCCTCCACCCGGCGCGTCCTCGGCCGCCGCGCGTTCCTGGA
GGACGTCCTGCTCCTCTCCGGCGGGGCGGTGGTCTTCCTCGCCGGCGCCACCGCCGCCGCGCCGGCCGCGGCGGAGGCGG
CCTCCGGCGCGCCGCCGCCCGCCGACGGCTACCAGCCCTGGGAGCACCGCTGGGCCTACCTCGTCGATCCGGAGCGGTGC
ATCGGCTGCGGCTCCTGCGTGCGGGCGTGCAGCGCCGAGAACCACGTGCCCGACGGCTTCTTCCGCACCTGGGTCGAGCG
CTACGTCACCGGCATGGACGGGACCAGCGTGGACTCGCCGAACGGCGGCAAGGACGGCTTCCCGGCGCTCCAGGTCGCGT
TCGTCGCGACGAAGTCGTTCTTCGTGCCGAAGATGTGCAACCACTGCCGCGAGACGCCCTGCATCCAGGTGTGCCCGGTG
GGCGCCTCCTACCGCACGCCCGACGGCGCGGTGCTGGTGGACGGCGAGCGCTGCATCGGGTGCGCGTACTGCGTGCAGGC
CTGCCCGTTCGGCAGCCGCTTCCTGTCGCCGGAGACGCACACGGCCGAGAAGTGCACCTGGTGCTACCACCGCATCAGCC
GCGGGCTGCGCCCGGCGTGCGTCGAGGTCTGCCCCACCGGCGCGCGCGCGTTCGGCGACCTGCGGCGCGAGGGCGATCCG
GTGCGACACGCCATCGAGCACGACCGCGTCGCGGTGCTGCAGCCGCACCTGCGCACCGAGCCGCAGTGCTTCTACCTCCA
CCTCGACGGGGAGGTGCGGTGA

Upstream 100 bases:

>100_bases
CGCAGGTGGTGCTCGAGGAGCACGTCGAGGGCCCGCTCGACGCCGGCGCCTGCCTCGCCTGCCACGACCCCCACGCTCCC
ACGCCGTAGCGGAGGCGATC

Downstream 100 bases:

>100_bases
TGCCCCAGATCAGCGGCTTCGCGTTCCCGAACGACGTCCACGTGTGGTGGAGCCTGATGATCGTGATGTACCCGTACATC
ACCGGCTTCGTGGCGGGCGC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MDDRTPCPASTGGPSTRRVLGRRAFLEDVLLLSGGAVVFLAGATAAAPAAAEAASGAPPPADGYQPWEHRWAYLVDPERC
IGCGSCVRACSAENHVPDGFFRTWVERYVTGMDGTSVDSPNGGKDGFPALQVAFVATKSFFVPKMCNHCRETPCIQVCPV
GASYRTPDGAVLVDGERCIGCAYCVQACPFGSRFLSPETHTAEKCTWCYHRISRGLRPACVEVCPTGARAFGDLRREGDP
VRHAIEHDRVAVLQPHLRTEPQCFYLHLDGEVR

Sequences:

>Translated_273_residues
MDDRTPCPASTGGPSTRRVLGRRAFLEDVLLLSGGAVVFLAGATAAAPAAAEAASGAPPPADGYQPWEHRWAYLVDPERC
IGCGSCVRACSAENHVPDGFFRTWVERYVTGMDGTSVDSPNGGKDGFPALQVAFVATKSFFVPKMCNHCRETPCIQVCPV
GASYRTPDGAVLVDGERCIGCAYCVQACPFGSRFLSPETHTAEKCTWCYHRISRGLRPACVEVCPTGARAFGDLRREGDP
VRHAIEHDRVAVLQPHLRTEPQCFYLHLDGEVR
>Mature_273_residues
MDDRTPCPASTGGPSTRRVLGRRAFLEDVLLLSGGAVVFLAGATAAAPAAAEAASGAPPPADGYQPWEHRWAYLVDPERC
IGCGSCVRACSAENHVPDGFFRTWVERYVTGMDGTSVDSPNGGKDGFPALQVAFVATKSFFVPKMCNHCRETPCIQVCPV
GASYRTPDGAVLVDGERCIGCAYCVQACPFGSRFLSPETHTAEKCTWCYHRISRGLRPACVEVCPTGARAFGDLRREGDP
VRHAIEHDRVAVLQPHLRTEPQCFYLHLDGEVR

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI226510944, Length=242, Percent_Identity=32.6446280991736, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI2367345, Length=206, Percent_Identity=34.9514563106796, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787872, Length=197, Percent_Identity=31.4720812182741, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787122, Length=197, Percent_Identity=30.9644670050761, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790326, Length=186, Percent_Identity=32.7956989247312, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787749, Length=186, Percent_Identity=31.1827956989247, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787740, Length=129, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787478, Length=117, Percent_Identity=34.1880341880342, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789370, Length=233, Percent_Identity=30.4721030042918, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367245, Length=166, Percent_Identity=31.3253012048193, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082180, Length=155, Percent_Identity=33.5483870967742, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082179, Length=178, Percent_Identity=31.4606741573034, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787963, Length=89, Percent_Identity=34.8314606741573, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788811, Length=90, Percent_Identity=42.2222222222222, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 29516; Mature: 29516

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRTPCPASTGGPSTRRVLGRRAFLEDVLLLSGGAVVFLAGATAAAPAAAEAASGAPPP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCCCCCCC
ADGYQPWEHRWAYLVDPERCIGCGSCVRACSAENHVPDGFFRTWVERYVTGMDGTSVDSP
CCCCCCHHHCEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
NGGKDGFPALQVAFVATKSFFVPKMCNHCRETPCIQVCPVGASYRTPDGAVLVDGERCIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCHHHH
CAYCVQACPFGSRFLSPETHTAEKCTWCYHRISRGLRPACVEVCPTGARAFGDLRREGDP
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCH
VRHAIEHDRVAVLQPHLRTEPQCFYLHLDGEVR
HHHHHHCCCEEEECCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MDDRTPCPASTGGPSTRRVLGRRAFLEDVLLLSGGAVVFLAGATAAAPAAAEAASGAPPP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCCCCCCC
ADGYQPWEHRWAYLVDPERCIGCGSCVRACSAENHVPDGFFRTWVERYVTGMDGTSVDSP
CCCCCCHHHCEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
NGGKDGFPALQVAFVATKSFFVPKMCNHCRETPCIQVCPVGASYRTPDGAVLVDGERCIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCHHHH
CAYCVQACPFGSRFLSPETHTAEKCTWCYHRISRGLRPACVEVCPTGARAFGDLRREGDP
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCH
VRHAIEHDRVAVLQPHLRTEPQCFYLHLDGEVR
HHHHHHCCCEEEECCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]