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The map label for this gene is ribH

Identifier: 220918022

GI number: 220918022

Start: 3237021

End: 3237509

Strand: Reverse

Name: ribH

Synonym: A2cp1_2923

Alternate gene names: 220918022

Gene position: 3237509-3237021 (Counterclockwise)

Preceding gene: 220918023

Following gene: 220918021

Centisome position: 64.37

GC content: 75.05

Gene sequence:

>489_bases
ATGAACGTCTACGAAGGCTCGCTGGTCGGCACCGGCCTCAAGGCCGCGCTGGTGGTGGCGCGCTTCAACTCGCTCGTCAC
CGAGCAGCTCCTGGTGGGCGCGGCCGACGCGCTCCGGCGGCACGGCGTCGCCGACGGCGACGTCGACGTGTTCCGCTGCC
CGGGCACGTTCGAGCTGCCGGCGGTCCTCCGGCGGGTGGTCGCGACCGGCCGGTACGACGCGGTCGTCGCGCTCGGCGCG
GTGATCCGGGGCGGCACGCCGCACTTCGAGTACGTGTCGGCCGAGGTCACGAAGGGCGTGGCCCACGTCGCCATGGAGGC
CGGCTGCGCGGTGACGATGGGCGTCCTCACCTGCGACAGCATGGAGCAGGCGCTGGAGCGCGCCGGCGTGAAGGCGGGCA
ACAAGGGCGCCGACGCCGCGGCCGCGGCGGTGGAGCAGGCCAACGTGCTGCGCGCGATCGCGCGCGCCCCCGCGCGGAAG
GCGGAGTGA

Upstream 100 bases:

>100_bases
CGGTCGCGGCGGCGGCGGCGGCACGGGCGCCGGCGGCCCCGGCGCGGGCCGCCAAGCGGCGGCGCCCGGCCCCCGCGTCC
AGGAAGGGAAAGGGAAGGCG

Downstream 100 bases:

>100_bases
CGGTGACGTCGCGGCGGACCAGGGCCCGGGAGCGCGCGCTGCAGGCGCTCTACCAGATCGACGTCGCGGCCGAGGGCATC
GACGACGCCCTCTCCCGCTT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MNVYEGSLVGTGLKAALVVARFNSLVTEQLLVGAADALRRHGVADGDVDVFRCPGTFELPAVLRRVVATGRYDAVVALGA
VIRGGTPHFEYVSAEVTKGVAHVAMEAGCAVTMGVLTCDSMEQALERAGVKAGNKGADAAAAAVEQANVLRAIARAPARK
AE

Sequences:

>Translated_162_residues
MNVYEGSLVGTGLKAALVVARFNSLVTEQLLVGAADALRRHGVADGDVDVFRCPGTFELPAVLRRVVATGRYDAVVALGA
VIRGGTPHFEYVSAEVTKGVAHVAMEAGCAVTMGVLTCDSMEQALERAGVKAGNKGADAAAAAVEQANVLRAIARAPARK
AE
>Mature_162_residues
MNVYEGSLVGTGLKAALVVARFNSLVTEQLLVGAADALRRHGVADGDVDVFRCPGTFELPAVLRRVVATGRYDAVVALGA
VIRGGTPHFEYVSAEVTKGVAHVAMEAGCAVTMGVLTCDSMEQALERAGVKAGNKGADAAAAAVEQANVLRAIARAPARK
AE

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=51.2987012987013, Blast_Score=156, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6324429, Length=155, Percent_Identity=34.8387096774194, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_ANAD2 (B8JEW4)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493326.1
- ProteinModelPortal:   B8JEW4
- SMR:   B8JEW4
- GeneID:   7299854
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2923
- HOGENOM:   HBG311126
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16661; Mature: 16661

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYEGSLVGTGLKAALVVARFNSLVTEQLLVGAADALRRHGVADGDVDVFRCPGTFELP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
AVLRRVVATGRYDAVVALGAVIRGGTPHFEYVSAEVTKGVAHVAMEAGCAVTMGVLTCDS
HHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MEQALERAGVKAGNKGADAAAAAVEQANVLRAIARAPARKAE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNVYEGSLVGTGLKAALVVARFNSLVTEQLLVGAADALRRHGVADGDVDVFRCPGTFELP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
AVLRRVVATGRYDAVVALGAVIRGGTPHFEYVSAEVTKGVAHVAMEAGCAVTMGVLTCDS
HHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MEQALERAGVKAGNKGADAAAAAVEQANVLRAIARAPARKAE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA