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The map label for this gene is prpE [H]

Identifier: 220917992

GI number: 220917992

Start: 3199678

End: 3200406

Strand: Direct

Name: prpE [H]

Synonym: A2cp1_2893

Alternate gene names: 220917992

Gene position: 3199678-3200406 (Clockwise)

Preceding gene: 220917991

Following gene: 220917996

Centisome position: 63.62

GC content: 76.95

Gene sequence:

>729_bases
ATGGCCCCCTCGCGCACTCTCGTCGTCGGCGACGTGCACGGCTGCCTGGGGGAGCTCGAGGACCTGCTCGCGGCCGCGCG
GTACGAGCCGATGCGCGATCGGCTGGTGTTCCTCGGCGACCTGGTGGACCGCGGCCCGGACCCGGTGGGCGTGGTCCGGC
GCGTCCGCGCGCTCGGCGCCGACTGCCTGCTCGGCAACCACGAGGAGAAGCACCTCCGCTACGCGGCGCACGAGGCACGG
CGCCGCGAGGACCCGCGCCACCGGAACCCCGTCCGGCTCGACCCCCGGCGCACCGAGGAGCACCTGCGGCTCTCGCCCGA
CGACCTGGCCTGGATGGCCGCGCTGCCGCGCGCGCTCCGGCTCGACGGCGGATGGGTCGCGGTCCACGGCGGGCTGCTGC
CGGGCCGCCCGCTTTCGCGCCAGCCGCCGGACTGGCTCATCCGGCTGCGCTTCCTCGACGCCACCGGCAAGCCGGTGTCG
CGGGAGCACGGCGGCGAGCCGGGCGTGGTGCGCTGGGCGGAGCGGTGGACCGGGCCGAGCTCGGTGGTCTACGGGCACCA
CGCGCGCGACGAGGTGGTGGTGGACGAGCCGGCCCCCGGCGTCCGGTGCGTCGGCATCGACACCGGGTGCGTGTACGGCG
GGAGGCTCACCGCGCTCGCGCTGCCGGGCGGCGAGCGCATCCAGGTGCCCTCGCGCCAGCGGAAGGCGACGCCCGAGCAG
GATGATTGA

Upstream 100 bases:

>100_bases
CCTCGGTCCAGGGCCGGGCCGGGCCGCAGCGCTGATGGCGCCTCGTTCGCGTGGAGTCCTTTCGCGGCACCGCCCTTCCG
CGGGGTACACTGCGCCCGCC

Downstream 100 bases:

>100_bases
GGCTAGCCCAGCTGCGCCGCGGCGGCGAGCTCGCCCTTGCGCTCGCGCGCGACCGCCACGTCGGCCTCGTACTTGAGCGC
GAGGTTGAGCGTCTGGTCGA

Product: metallophosphoesterase

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAPSRTLVVGDVHGCLGELEDLLAAARYEPMRDRLVFLGDLVDRGPDPVGVVRRVRALGADCLLGNHEEKHLRYAAHEAR
RREDPRHRNPVRLDPRRTEEHLRLSPDDLAWMAALPRALRLDGGWVAVHGGLLPGRPLSRQPPDWLIRLRFLDATGKPVS
REHGGEPGVVRWAERWTGPSSVVYGHHARDEVVVDEPAPGVRCVGIDTGCVYGGRLTALALPGGERIQVPSRQRKATPEQ
DD

Sequences:

>Translated_242_residues
MAPSRTLVVGDVHGCLGELEDLLAAARYEPMRDRLVFLGDLVDRGPDPVGVVRRVRALGADCLLGNHEEKHLRYAAHEAR
RREDPRHRNPVRLDPRRTEEHLRLSPDDLAWMAALPRALRLDGGWVAVHGGLLPGRPLSRQPPDWLIRLRFLDATGKPVS
REHGGEPGVVRWAERWTGPSSVVYGHHARDEVVVDEPAPGVRCVGIDTGCVYGGRLTALALPGGERIQVPSRQRKATPEQ
DD
>Mature_241_residues
APSRTLVVGDVHGCLGELEDLLAAARYEPMRDRLVFLGDLVDRGPDPVGVVRRVRALGADCLLGNHEEKHLRYAAHEARR
REDPRHRNPVRLDPRRTEEHLRLSPDDLAWMAALPRALRLDGGWVAVHGGLLPGRPLSRQPPDWLIRLRFLDATGKPVSR
EHGGEPGVVRWAERWTGPSSVVYGHHARDEVVVDEPAPGVRCVGIDTGCVYGGRLTALALPGGERIQVPSRQRKATPEQD
D

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.6.1.17 [H]

Molecular weight: Translated: 26826; Mature: 26695

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSRTLVVGDVHGCLGELEDLLAAARYEPMRDRLVFLGDLVDRGPDPVGVVRRVRALGA
CCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
DCLLGNHEEKHLRYAAHEARRREDPRHRNPVRLDPRRTEEHLRLSPDDLAWMAALPRALR
HHEECCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHCCCCCHHHHHHHHCCHHEE
LDGGWVAVHGGLLPGRPLSRQPPDWLIRLRFLDATGKPVSREHGGEPGVVRWAERWTGPS
ECCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCEEHHHHHCCCCC
SVVYGHHARDEVVVDEPAPGVRCVGIDTGCVYGGRLTALALPGGERIQVPSRQRKATPEQ
CEEECCCCCCCEEEECCCCCEEEEEECCCEEECCEEEEEECCCCCEEECCCHHHCCCCCC
DD
CC
>Mature Secondary Structure 
APSRTLVVGDVHGCLGELEDLLAAARYEPMRDRLVFLGDLVDRGPDPVGVVRRVRALGA
CCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
DCLLGNHEEKHLRYAAHEARRREDPRHRNPVRLDPRRTEEHLRLSPDDLAWMAALPRALR
HHEECCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHCCCCCHHHHHHHHCCHHEE
LDGGWVAVHGGLLPGRPLSRQPPDWLIRLRFLDATGKPVSREHGGEPGVVRWAERWTGPS
ECCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCEEHHHHHCCCCC
SVVYGHHARDEVVVDEPAPGVRCVGIDTGCVYGGRLTALALPGGERIQVPSRQRKATPEQ
CEEECCCCCCCEEEECCCCCEEEEEECCCEEECCEEEEEECCCCCEEECCCHHHCCCCCC
DD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]