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The map label for this gene is trmFO

Identifier: 220917982

GI number: 220917982

Start: 3184466

End: 3185827

Strand: Reverse

Name: trmFO

Synonym: A2cp1_2883

Alternate gene names: 220917982

Gene position: 3185827-3184466 (Counterclockwise)

Preceding gene: 220917984

Following gene: 220917981

Centisome position: 63.34

GC content: 75.33

Gene sequence:

>1362_bases
ATGGGCGACACGCGGGTGACCGTGATCGGGGGAGGGCTGGCGGGGACCGAGGCGGCGTGGCAGCTCGCGCGCGCTGGCGT
CGCCGTCGAGCTCGTGGAGATGAAGCCCGAGCGCCGCTCGCCGGCGCACGTCCTGCCCGGCCTCGCCGAGCTGGTCTGCT
CCAACTCGCTCCGGTCCGACAACCCGCTCAACGCGGTCGGCCTGCTCCACGAGGAGCTGCGCCGCCTCGGCAGCCTCGTG
CTCGGCTGCGCCGACGAGACGCGCGTGCCGGCCGGTGACGCGCTCGCGGTCGACCGCGAGCGGTTCAGCGAGGCGGTCAC
CGCGCGGCTCACGGGTCACGCCGGCGTGCGGATCGTCCACCGCGAGTTGGAGGAGCTGCCGCCGCCGCCCGCGCTGGCGG
TGATCGCCACCGGGCCGCTCACCGCCGACGCGCTCGCGGCGCGCCTCGCCGAGACCACCGGCGGGCGCCTGCACTTCTAC
GACGCCATCGCGCCCATCGTGGCGGCGGAGTCGATCGACCGGAGCATCGCGTACGCGCGCTCCCGCTACGGCAAGGGGAG
CGGCGACGACTACCTGAACCTGCCGCTCGACGAGGCGCAGTACCACGCGTTCGTGGAGGCGCTGCTCCAGGGCGAGAAGG
TCGCTGCGCACGGGTTCGAGGAGCCGCGCTACTTCGAGGGCTGCCTGCCGATCGAGGTCATGGCGGAGCGCGGGCTGGAG
GTCCTCGCCCATGGCCCGCTGAAGCCGGTGGGGCTCGAGGACCCGCGCACCGGGCGCCGGCCGCACGCCGTGGTGCAGCT
CCGCCGCGAGGACGTGGACGGCACCGCCTGGAACCTGGTCGGTTTCCAGACCCGCCTCACCTGGCCGGAGCAGCGCCGGA
TCTTCCGCGCCTTCCTCCCCGGCCTCGCGAACGCCGAGTTCGTCCGGCTCGGCCAGATCCACCGCAACACGTTCGTGGAC
GCCCCGCGCGTCCTCGCGCCGGACCTGTCGGTTCGCGCCGCCCCGCACCTGTTCCTGGCCGGGCAGATCACCGGGGTGGA
AGGGTACGTGGAGTCGGCCGCCTGCGGGCTGATGGCGGCGCGGGCGGTGCTGGACCGGCTGGCGGGGCGCGCCTTCCGGC
CGCCCCCGCCGGCGACCGCCCTCGGCGCCCTCCATCGACATCTCACCGGCGAGGCGCATCCGCCGGGGTACGATTACCAG
CCGAGCAACGTGGTCTTCGCGCTGTTCCCTCCGCTCACCGGGCGCCACCGCGGCAAGGCCGGCCGCAAGGAGGCCCACGT
GGAGCGGGCGCGCAAGGAGCTGGCACCGTGGATCGACTCCGCCCCGCCCACCGCCGTCCCCGCGGCTCCCGCCGCGGGCT
GA

Upstream 100 bases:

>100_bases
CCCTGCGGGCGCGCGGCCAGGACGGTCGCCCGGACGAGGCGACGGAGAGCGCCTGCAGGGCGGGCGACCGGCCGGGGCGG
ACGGAGACGGAAAGGGGCGC

Downstream 100 bases:

>100_bases
CCCGCGCCGCCGCGCTGGCGCTCGCGCTCGCCGCCGCCGGCTGCCCCGCGAAGCAGGAGCCGGGCGCCGCCGCCGGGAGC
GCCGCGGGGCGCGCCGTGCT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MGDTRVTVIGGGLAGTEAAWQLARAGVAVELVEMKPERRSPAHVLPGLAELVCSNSLRSDNPLNAVGLLHEELRRLGSLV
LGCADETRVPAGDALAVDRERFSEAVTARLTGHAGVRIVHRELEELPPPPALAVIATGPLTADALAARLAETTGGRLHFY
DAIAPIVAAESIDRSIAYARSRYGKGSGDDYLNLPLDEAQYHAFVEALLQGEKVAAHGFEEPRYFEGCLPIEVMAERGLE
VLAHGPLKPVGLEDPRTGRRPHAVVQLRREDVDGTAWNLVGFQTRLTWPEQRRIFRAFLPGLANAEFVRLGQIHRNTFVD
APRVLAPDLSVRAAPHLFLAGQITGVEGYVESAACGLMAARAVLDRLAGRAFRPPPPATALGALHRHLTGEAHPPGYDYQ
PSNVVFALFPPLTGRHRGKAGRKEAHVERARKELAPWIDSAPPTAVPAAPAAG

Sequences:

>Translated_453_residues
MGDTRVTVIGGGLAGTEAAWQLARAGVAVELVEMKPERRSPAHVLPGLAELVCSNSLRSDNPLNAVGLLHEELRRLGSLV
LGCADETRVPAGDALAVDRERFSEAVTARLTGHAGVRIVHRELEELPPPPALAVIATGPLTADALAARLAETTGGRLHFY
DAIAPIVAAESIDRSIAYARSRYGKGSGDDYLNLPLDEAQYHAFVEALLQGEKVAAHGFEEPRYFEGCLPIEVMAERGLE
VLAHGPLKPVGLEDPRTGRRPHAVVQLRREDVDGTAWNLVGFQTRLTWPEQRRIFRAFLPGLANAEFVRLGQIHRNTFVD
APRVLAPDLSVRAAPHLFLAGQITGVEGYVESAACGLMAARAVLDRLAGRAFRPPPPATALGALHRHLTGEAHPPGYDYQ
PSNVVFALFPPLTGRHRGKAGRKEAHVERARKELAPWIDSAPPTAVPAAPAAG
>Mature_452_residues
GDTRVTVIGGGLAGTEAAWQLARAGVAVELVEMKPERRSPAHVLPGLAELVCSNSLRSDNPLNAVGLLHEELRRLGSLVL
GCADETRVPAGDALAVDRERFSEAVTARLTGHAGVRIVHRELEELPPPPALAVIATGPLTADALAARLAETTGGRLHFYD
AIAPIVAAESIDRSIAYARSRYGKGSGDDYLNLPLDEAQYHAFVEALLQGEKVAAHGFEEPRYFEGCLPIEVMAERGLEV
LAHGPLKPVGLEDPRTGRRPHAVVQLRREDVDGTAWNLVGFQTRLTWPEQRRIFRAFLPGLANAEFVRLGQIHRNTFVDA
PRVLAPDLSVRAAPHLFLAGQITGVEGYVESAACGLMAARAVLDRLAGRAFRPPPPATALGALHRHLTGEAHPPGYDYQP
SNVVFALFPPLTGRHRGKAGRKEAHVERARKELAPWIDSAPPTAVPAAPAAG

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_ANAD2 (B8JES4)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493286.1
- GeneID:   7299814
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2883
- HOGENOM:   HBG285041
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 48626; Mature: 48495

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDTRVTVIGGGLAGTEAAWQLARAGVAVELVEMKPERRSPAHVLPGLAELVCSNSLRSD
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
NPLNAVGLLHEELRRLGSLVLGCADETRVPAGDALAVDRERFSEAVTARLTGHAGVRIVH
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHH
RELEELPPPPALAVIATGPLTADALAARLAETTGGRLHFYDAIAPIVAAESIDRSIAYAR
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SRYGKGSGDDYLNLPLDEAQYHAFVEALLQGEKVAAHGFEEPRYFEGCLPIEVMAERGLE
HHCCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHCCCCCHHHHHHCCCC
VLAHGPLKPVGLEDPRTGRRPHAVVQLRREDVDGTAWNLVGFQTRLTWPEQRRIFRAFLP
EEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHC
GLANAEFVRLGQIHRNTFVDAPRVLAPDLSVRAAPHLFLAGQITGVEGYVESAACGLMAA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHH
RAVLDRLAGRAFRPPPPATALGALHRHLTGEAHPPGYDYQPSNVVFALFPPLTGRHRGKA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
GRKEAHVERARKELAPWIDSAPPTAVPAAPAAG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GDTRVTVIGGGLAGTEAAWQLARAGVAVELVEMKPERRSPAHVLPGLAELVCSNSLRSD
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
NPLNAVGLLHEELRRLGSLVLGCADETRVPAGDALAVDRERFSEAVTARLTGHAGVRIVH
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHH
RELEELPPPPALAVIATGPLTADALAARLAETTGGRLHFYDAIAPIVAAESIDRSIAYAR
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SRYGKGSGDDYLNLPLDEAQYHAFVEALLQGEKVAAHGFEEPRYFEGCLPIEVMAERGLE
HHCCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHCCCCCHHHHHHCCCC
VLAHGPLKPVGLEDPRTGRRPHAVVQLRREDVDGTAWNLVGFQTRLTWPEQRRIFRAFLP
EEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHC
GLANAEFVRLGQIHRNTFVDAPRVLAPDLSVRAAPHLFLAGQITGVEGYVESAACGLMAA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHH
RAVLDRLAGRAFRPPPPATALGALHRHLTGEAHPPGYDYQPSNVVFALFPPLTGRHRGKA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
GRKEAHVERARKELAPWIDSAPPTAVPAAPAAG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA