Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 220917961

GI number: 220917961

Start: 3165837

End: 3166739

Strand: Reverse

Name: metF [H]

Synonym: A2cp1_2862

Alternate gene names: 220917961

Gene position: 3166739-3165837 (Counterclockwise)

Preceding gene: 220917966

Following gene: 220917959

Centisome position: 62.97

GC content: 73.75

Gene sequence:

>903_bases
GTGCGGATCACCGACCTCCTGCGCTTCGCCCGAGAGCGGGGCGAGCCCATCTTCTCCTTCGAGTTCTTCCCGCCCAAGAC
CGACGACGGGGTGCGAGGCCTGTTCGAGACGGTCGAGGCGCTGCGCCCGCTCGGCCCCGCCTACGTGTCGGTGACGTACG
GCGCCGGCGGGTCCACCCGGGCGAAGACCATCGAGCTGGTGAAGCGGCTCAAGCGCGACGCCGAGGTCGAGGCGATGGCG
CACGTCACCTGCGTGGGCGCGTCGCGCGACGAGATCGCGGCCGTGCTCGACGAGGTGGCGGAGGCCGGGATCCAGAACGT
GCTGGCGCTGCGCGGCGATCCGCCGCGCGGGCAGGGCGGGTTCGTCCCGCACCCGGACGGCTTCACGCACGCGAGCGAGC
TCGTCGCGTTCATCCGGTCGCAGCCGGAGCGCTGGCGCTTCTGCGTGGGCGCCGCCGCGTACCCCGAGGGCCACCCCGAG
ACCCGGGACCTGGCGCAGGACCTCCGCCACCTGAAGCTCAAGGTCGACGCCGGCAGCGACTTCCTGGTGACGCAGCTGTT
CTTCGAGAACGCGCACTACTTCCGCTTCGTCGAGCGCGCGCGCGCCGGCGGCGTCGAGGTGCCGGTGGTGCCCGGGATCA
TGCCGTTCACCAACGTCGAGCAGGTGGAGCGCTTCACCGCCATGTGCGGCGCCGCCATCCCGCCGCCGCTCCGCGCGGCG
ATGGAGGTCCGGCGCGCGGACCCGGAGAGCGCCCGCGAGCTGGGCGTGGCCTACGCGACGCTGCAGTGCGCGGACCTGCT
GCGCCGCGGCGCCCCCGGGATCCACTTCTACACGCTGAACCGCTCGCCCTCGACGCGTGCCATCGTGGCGGCGCTCCGGG
CCTGCGAGCCCTGGCGCGGCTGA

Upstream 100 bases:

>100_bases
TGGACATGGTGGTGCGACGAACGATTCCCGCGGAGCGGCGCCGCTGTCAACCGCAGGAAGCTGGCGCCGCGCGGCCGGGC
CGGGCTACTCTCGCGGGCCT

Downstream 100 bases:

>100_bases
TCAGTGCAGCGTGCCGGTCCACTCGTCGTGGACCACCATCACCGGGCAGCGCGCCCGCCGGACCGTGCTCTCCGCCACCG
AGCCCACCAGCGCGTGCGTC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRITDLLRFARERGEPIFSFEFFPPKTDDGVRGLFETVEALRPLGPAYVSVTYGAGGSTRAKTIELVKRLKRDAEVEAMA
HVTCVGASRDEIAAVLDEVAEAGIQNVLALRGDPPRGQGGFVPHPDGFTHASELVAFIRSQPERWRFCVGAAAYPEGHPE
TRDLAQDLRHLKLKVDAGSDFLVTQLFFENAHYFRFVERARAGGVEVPVVPGIMPFTNVEQVERFTAMCGAAIPPPLRAA
MEVRRADPESARELGVAYATLQCADLLRRGAPGIHFYTLNRSPSTRAIVAALRACEPWRG

Sequences:

>Translated_300_residues
MRITDLLRFARERGEPIFSFEFFPPKTDDGVRGLFETVEALRPLGPAYVSVTYGAGGSTRAKTIELVKRLKRDAEVEAMA
HVTCVGASRDEIAAVLDEVAEAGIQNVLALRGDPPRGQGGFVPHPDGFTHASELVAFIRSQPERWRFCVGAAAYPEGHPE
TRDLAQDLRHLKLKVDAGSDFLVTQLFFENAHYFRFVERARAGGVEVPVVPGIMPFTNVEQVERFTAMCGAAIPPPLRAA
MEVRRADPESARELGVAYATLQCADLLRRGAPGIHFYTLNRSPSTRAIVAALRACEPWRG
>Mature_300_residues
MRITDLLRFARERGEPIFSFEFFPPKTDDGVRGLFETVEALRPLGPAYVSVTYGAGGSTRAKTIELVKRLKRDAEVEAMA
HVTCVGASRDEIAAVLDEVAEAGIQNVLALRGDPPRGQGGFVPHPDGFTHASELVAFIRSQPERWRFCVGAAAYPEGHPE
TRDLAQDLRHLKLKVDAGSDFLVTQLFFENAHYFRFVERARAGGVEVPVVPGIMPFTNVEQVERFTAMCGAAIPPPLRAA
MEVRRADPESARELGVAYATLQCADLLRRGAPGIHFYTLNRSPSTRAIVAALRACEPWRG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=296, Percent_Identity=34.4594594594595, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1790377, Length=284, Percent_Identity=36.2676056338028, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI25147441, Length=287, Percent_Identity=32.404181184669, Blast_Score=161, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25147436, Length=287, Percent_Identity=32.404181184669, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325234, Length=305, Percent_Identity=36.0655737704918, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6321313, Length=299, Percent_Identity=32.1070234113712, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24662658, Length=270, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32972; Mature: 32972

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITDLLRFARERGEPIFSFEFFPPKTDDGVRGLFETVEALRPLGPAYVSVTYGAGGSTR
CCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
AKTIELVKRLKRDAEVEAMAHVTCVGASRDEIAAVLDEVAEAGIQNVLALRGDPPRGQGG
HHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FVPHPDGFTHASELVAFIRSQPERWRFCVGAAAYPEGHPETRDLAQDLRHLKLKVDAGSD
CCCCCCCCHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCCHHHHHHHHHHEEEEECCCCC
FLVTQLFFENAHYFRFVERARAGGVEVPVVPGIMPFTNVEQVERFTAMCGAAIPPPLRAA
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
MEVRRADPESARELGVAYATLQCADLLRRGAPGIHFYTLNRSPSTRAIVAALRACEPWRG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRITDLLRFARERGEPIFSFEFFPPKTDDGVRGLFETVEALRPLGPAYVSVTYGAGGSTR
CCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
AKTIELVKRLKRDAEVEAMAHVTCVGASRDEIAAVLDEVAEAGIQNVLALRGDPPRGQGG
HHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FVPHPDGFTHASELVAFIRSQPERWRFCVGAAAYPEGHPETRDLAQDLRHLKLKVDAGSD
CCCCCCCCHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCCHHHHHHHHHHEEEEECCCCC
FLVTQLFFENAHYFRFVERARAGGVEVPVVPGIMPFTNVEQVERFTAMCGAAIPPPLRAA
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
MEVRRADPESARELGVAYATLQCADLLRRGAPGIHFYTLNRSPSTRAIVAALRACEPWRG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]