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The map label for this gene is phsA [H]

Identifier: 220917912

GI number: 220917912

Start: 3109129

End: 3111345

Strand: Reverse

Name: phsA [H]

Synonym: A2cp1_2813

Alternate gene names: 220917912

Gene position: 3111345-3109129 (Counterclockwise)

Preceding gene: 220917919

Following gene: 220917911

Centisome position: 61.86

GC content: 74.24

Gene sequence:

>2217_bases
ATGGCGGAGGACGGCGTGCGCATCGGGCGGAGGACGTTCCTGGAGCTGGCGGCGGCGGGCGCGGCCGCGGGCGCCGCGGC
GGCGCCCGATCGCGCGGAGGCGTTCGGCCTCGCGCAGGCGGACGACGCCCTGCCGCCGGAGCGCGAGGTCCCCACCTTCT
GCGAGCTGTGCTTCTGGAACTGCGGGGCGGTGGCGCGCGTGCGGAAGAACCGCGTGCACTCGCTGCGCGGCCACCCGCGG
TATCCGAACGCGAACGGGAAGCTGTGCGGGCGCGGGCAGGCCGGCGCGGCGTCGGTCGTGGACGCGGACCGGCTCACGCA
CCCGCTGATCCGGACCGGCGCACGCGGCGAGGGGAAGTTCCGCCGCGCCGGGTGGAACGAGGCCTACCAGATCGTCGCGC
GGGGCTTCGGCGACATCAAGGCGCGCCACGGCGCCGAGGCGCTCGCGCTCTTCTATCACGGCAGCGGCGGGCCCCTGCTC
CGGCAGATGCTGGTGGCGTACGGCTCGCCCAACTACGCGGGCCCCTCGTACGCGCAGTGCAAGGGCGCCCGCAACGTCGG
CTACCAGCTCACGTTCGGCGAGAAGCTGGTCTCGCCCGAGCCGATCGACCTGGAGAACGCGCGCGCGGTCGTGCTGTTCG
GGTCGCATCTCGGCGAGAACGCGCACAACGGCCAGGTGCAGGAGCTGGTGCGGGCGCGGGTCCGCGGCGCGTCCCTGGTG
GTGCTGGATCCGCGCCTCTCCACCGTGGCCTCGAAGGCCGACGTCTGGCTGCCCATCCGCCCGGGCGCCGACACCGCGGT
GATCCTGGCCTGGCTCCACCTGCTCGTCGCGGAGCGCACCTACGATCGCGCCTTCGTGGAGGCCCGCACCACCGGCTTCG
ACCGGCTGGCGGCCCACGTGGCGCCGTTCGACCCGGCCTGGGCGGCCGAGCGCGCCGGCGTGCCGGCGCAGCGGATCGTG
GACGCGTATCGCCTGATGGTGAACGCGATGCCGGCGGTGGTCGTCCACCCCGGCCGGCACACCGCCTGGTACGGCGAGGA
GGACACGCAGCGCGCGCGCGCCCAGGCGATCCTCTCCGCGCTGCTCGGCGCCTGGTGGCGACCCGGGTCGACCTACCGGA
CCGAGCGCGCGACCGTCGACGACTACCCCACGCCGGACTTCCCGGACCTGCCCCGGAACGTGGACCAGGCCGCCGGCCGC
TTCCCGTTCGCGGAGGAGGTCACCACCAACGGCCTTCGCGACGCCACCCGCACCGGCAAGCCGTACCCGGTGAAGGGCTG
GTTCGTGCACGGCACGAACCTGGTGCAGGCGATGCCCAACGTGCGCGAGACGCGCGAGGCGATCGCGAACCTCGACCTGC
TGGTGGTCTGCGACGTCGCGCCCACCGAGATCACCCAGTACGCCGACGTGCTGCTCCCGGAGGATACCTACCTCGAGCGC
TACGACGACCTCTCGCTCGGCTTCACGCGCACGCCGTACGTGGGCCTGCGCCAGCCGGCGGTGGCCTCGCCGCACGACAC
GCGGCCCGCGTGGCGGATCGCGAAGGAGCTCGGGACCGCGCTCGGCGTGGGCGACTTCTTCCCGTGGGCGACGTTCGAGG
AGTACCTCGAGGTCCGTCTCGCCTCCGCCGGCCTGTCGCTCGACCAGCTGAGGCGCGACGGGATCGCGCTCGTCCCCCGC
AAGACGCCGCTCTACCTGGGCGACGGCGAGGCGTACCGCTTCCACACGCCGTCCGGCAGGATCGAGCTCTGGTCGCAGCG
GCTCGCCGACGCGGGCTTCGACCCGCTCCCGGTGTGGAAGGGACCCCCGCCGCCGCCGCCCGGGCGCTTCCGGCTGCTCT
ACGGCCGCAGCCCGCTCCACACGTTCGGCCGCACCCAGAACAACCCCATCCTGTTCGACCTCGAGCCCGAGAACACGCTC
TGGATGAGCCCGGCGGACGCGACCGCGCTCGGCCTCGCGGCCGGGGAGCGGGTCATGGTGGGCAACGCCCACGGCAGCGA
GACCGGACCGCTCGCGCTGCACGTCACCGAGCGGATCCCGGACGGCGCCGTGTACATGGTCCACGGCTTCGGCCACTGGG
CCCACCGGCTCCACCGGGCGCGCGGGCGCGGCGGCGACGACGGCGCGGTGATCGACGACTACGTGGTCGACCCCATCAGC
GGCTCGACCGGCATGCGCACCCAGCTCGTCACGGTGCGCCGGGCGGAGGTTGCCTGA

Upstream 100 bases:

>100_bases
GCTGAAATCACACGCGAAAATCGGATCCGGGCGAGAATCCACCGCGGCCGTTCGCGGCTCCATCGCGGTGGTCGGCGGTG
CCGCCGGCGGGGAGCGCGAG

Downstream 100 bases:

>100_bases
TGATGGCGCCGCCCGTCCGCGTAGAGCCCTGCTGCGGCGCACGCTGGCCGCCTGCGCCGGGCAGGCTCGTCCCTGCGCTG
CTCGACGTGCCGACGGGCAC

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MAEDGVRIGRRTFLELAAAGAAAGAAAAPDRAEAFGLAQADDALPPEREVPTFCELCFWNCGAVARVRKNRVHSLRGHPR
YPNANGKLCGRGQAGAASVVDADRLTHPLIRTGARGEGKFRRAGWNEAYQIVARGFGDIKARHGAEALALFYHGSGGPLL
RQMLVAYGSPNYAGPSYAQCKGARNVGYQLTFGEKLVSPEPIDLENARAVVLFGSHLGENAHNGQVQELVRARVRGASLV
VLDPRLSTVASKADVWLPIRPGADTAVILAWLHLLVAERTYDRAFVEARTTGFDRLAAHVAPFDPAWAAERAGVPAQRIV
DAYRLMVNAMPAVVVHPGRHTAWYGEEDTQRARAQAILSALLGAWWRPGSTYRTERATVDDYPTPDFPDLPRNVDQAAGR
FPFAEEVTTNGLRDATRTGKPYPVKGWFVHGTNLVQAMPNVRETREAIANLDLLVVCDVAPTEITQYADVLLPEDTYLER
YDDLSLGFTRTPYVGLRQPAVASPHDTRPAWRIAKELGTALGVGDFFPWATFEEYLEVRLASAGLSLDQLRRDGIALVPR
KTPLYLGDGEAYRFHTPSGRIELWSQRLADAGFDPLPVWKGPPPPPPGRFRLLYGRSPLHTFGRTQNNPILFDLEPENTL
WMSPADATALGLAAGERVMVGNAHGSETGPLALHVTERIPDGAVYMVHGFGHWAHRLHRARGRGGDDGAVIDDYVVDPIS
GSTGMRTQLVTVRRAEVA

Sequences:

>Translated_738_residues
MAEDGVRIGRRTFLELAAAGAAAGAAAAPDRAEAFGLAQADDALPPEREVPTFCELCFWNCGAVARVRKNRVHSLRGHPR
YPNANGKLCGRGQAGAASVVDADRLTHPLIRTGARGEGKFRRAGWNEAYQIVARGFGDIKARHGAEALALFYHGSGGPLL
RQMLVAYGSPNYAGPSYAQCKGARNVGYQLTFGEKLVSPEPIDLENARAVVLFGSHLGENAHNGQVQELVRARVRGASLV
VLDPRLSTVASKADVWLPIRPGADTAVILAWLHLLVAERTYDRAFVEARTTGFDRLAAHVAPFDPAWAAERAGVPAQRIV
DAYRLMVNAMPAVVVHPGRHTAWYGEEDTQRARAQAILSALLGAWWRPGSTYRTERATVDDYPTPDFPDLPRNVDQAAGR
FPFAEEVTTNGLRDATRTGKPYPVKGWFVHGTNLVQAMPNVRETREAIANLDLLVVCDVAPTEITQYADVLLPEDTYLER
YDDLSLGFTRTPYVGLRQPAVASPHDTRPAWRIAKELGTALGVGDFFPWATFEEYLEVRLASAGLSLDQLRRDGIALVPR
KTPLYLGDGEAYRFHTPSGRIELWSQRLADAGFDPLPVWKGPPPPPPGRFRLLYGRSPLHTFGRTQNNPILFDLEPENTL
WMSPADATALGLAAGERVMVGNAHGSETGPLALHVTERIPDGAVYMVHGFGHWAHRLHRARGRGGDDGAVIDDYVVDPIS
GSTGMRTQLVTVRRAEVA
>Mature_737_residues
AEDGVRIGRRTFLELAAAGAAAGAAAAPDRAEAFGLAQADDALPPEREVPTFCELCFWNCGAVARVRKNRVHSLRGHPRY
PNANGKLCGRGQAGAASVVDADRLTHPLIRTGARGEGKFRRAGWNEAYQIVARGFGDIKARHGAEALALFYHGSGGPLLR
QMLVAYGSPNYAGPSYAQCKGARNVGYQLTFGEKLVSPEPIDLENARAVVLFGSHLGENAHNGQVQELVRARVRGASLVV
LDPRLSTVASKADVWLPIRPGADTAVILAWLHLLVAERTYDRAFVEARTTGFDRLAAHVAPFDPAWAAERAGVPAQRIVD
AYRLMVNAMPAVVVHPGRHTAWYGEEDTQRARAQAILSALLGAWWRPGSTYRTERATVDDYPTPDFPDLPRNVDQAAGRF
PFAEEVTTNGLRDATRTGKPYPVKGWFVHGTNLVQAMPNVRETREAIANLDLLVVCDVAPTEITQYADVLLPEDTYLERY
DDLSLGFTRTPYVGLRQPAVASPHDTRPAWRIAKELGTALGVGDFFPWATFEEYLEVRLASAGLSLDQLRRDGIALVPRK
TPLYLGDGEAYRFHTPSGRIELWSQRLADAGFDPLPVWKGPPPPPPGRFRLLYGRSPLHTFGRTQNNPILFDLEPENTLW
MSPADATALGLAAGERVMVGNAHGSETGPLALHVTERIPDGAVYMVHGFGHWAHRLHRARGRGGDDGAVIDDYVVDPISG
STGMRTQLVTVRRAEVA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=825, Percent_Identity=26.5454545454545, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1787870, Length=836, Percent_Identity=27.8708133971292, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI171474008, Length=776, Percent_Identity=27.1907216494845, Blast_Score=179, Evalue=8e-46,
Organism=Escherichia coli, GI3868721, Length=572, Percent_Identity=23.6013986013986, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI3868720, Length=315, Percent_Identity=27.6190476190476, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI3868719, Length=310, Percent_Identity=27.0967741935484, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1787231, Length=670, Percent_Identity=21.9402985074627, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081994, Length=685, Percent_Identity=23.3576642335766, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788534, Length=503, Percent_Identity=21.4711729622266, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 80502; Mature: 80371

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDGVRIGRRTFLELAAAGAAAGAAAAPDRAEAFGLAQADDALPPEREVPTFCELCFWN
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHC
CGAVARVRKNRVHSLRGHPRYPNANGKLCGRGQAGAASVVDADRLTHPLIRTGARGEGKF
CCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC
RRAGWNEAYQIVARGFGDIKARHGAEALALFYHGSGGPLLRQMLVAYGSPNYAGPSYAQC
CCCCHHHHHHHHHHCCCHHHHHCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHH
KGARNVGYQLTFGEKLVSPEPIDLENARAVVLFGSHLGENAHNGQVQELVRARVRGASLV
CCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEE
VLDPRLSTVASKADVWLPIRPGADTAVILAWLHLLVAERTYDRAFVEARTTGFDRLAAHV
EECCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
APFDPAWAAERAGVPAQRIVDAYRLMVNAMPAVVVHPGRHTAWYGEEDTQRARAQAILSA
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LLGAWWRPGSTYRTERATVDDYPTPDFPDLPRNVDQAAGRFPFAEEVTTNGLRDATRTGK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHCCCHHHHHCCC
PYPVKGWFVHGTNLVQAMPNVRETREAIANLDLLVVCDVAPTEITQYADVLLPEDTYLER
CCCCEEEEEECCHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCHHHHHH
YDDLSLGFTRTPYVGLRQPAVASPHDTRPAWRIAKELGTALGVGDFFPWATFEEYLEVRL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ASAGLSLDQLRRDGIALVPRKTPLYLGDGEAYRFHTPSGRIELWSQRLADAGFDPLPVWK
HHCCCCHHHHHCCCEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
GPPPPPPGRFRLLYGRSPLHTFGRTQNNPILFDLEPENTLWMSPADATALGLAAGERVMV
CCCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEECCCCEEEECCCCCHHHHHCCCCEEEE
GNAHGSETGPLALHVTERIPDGAVYMVHGFGHWAHRLHRARGRGGDDGAVIDDYVVDPIS
ECCCCCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEECCEEECCCC
GSTGMRTQLVTVRRAEVA
CCCCCEEEEEEEEHHCCC
>Mature Secondary Structure 
AEDGVRIGRRTFLELAAAGAAAGAAAAPDRAEAFGLAQADDALPPEREVPTFCELCFWN
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHC
CGAVARVRKNRVHSLRGHPRYPNANGKLCGRGQAGAASVVDADRLTHPLIRTGARGEGKF
CCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC
RRAGWNEAYQIVARGFGDIKARHGAEALALFYHGSGGPLLRQMLVAYGSPNYAGPSYAQC
CCCCHHHHHHHHHHCCCHHHHHCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHH
KGARNVGYQLTFGEKLVSPEPIDLENARAVVLFGSHLGENAHNGQVQELVRARVRGASLV
CCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEE
VLDPRLSTVASKADVWLPIRPGADTAVILAWLHLLVAERTYDRAFVEARTTGFDRLAAHV
EECCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
APFDPAWAAERAGVPAQRIVDAYRLMVNAMPAVVVHPGRHTAWYGEEDTQRARAQAILSA
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LLGAWWRPGSTYRTERATVDDYPTPDFPDLPRNVDQAAGRFPFAEEVTTNGLRDATRTGK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHCCCHHHHHCCC
PYPVKGWFVHGTNLVQAMPNVRETREAIANLDLLVVCDVAPTEITQYADVLLPEDTYLER
CCCCEEEEEECCHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCHHHHHH
YDDLSLGFTRTPYVGLRQPAVASPHDTRPAWRIAKELGTALGVGDFFPWATFEEYLEVRL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ASAGLSLDQLRRDGIALVPRKTPLYLGDGEAYRFHTPSGRIELWSQRLADAGFDPLPVWK
HHCCCCHHHHHCCCEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
GPPPPPPGRFRLLYGRSPLHTFGRTQNNPILFDLEPENTLWMSPADATALGLAAGERVMV
CCCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEECCCCEEEECCCCCHHHHHCCCCEEEE
GNAHGSETGPLALHVTERIPDGAVYMVHGFGHWAHRLHRARGRGGDDGAVIDDYVVDPIS
ECCCCCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEECCEEECCCC
GSTGMRTQLVTVRRAEVA
CCCCCEEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]