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The map label for this gene is fusA [H]

Identifier: 220917898

GI number: 220917898

Start: 3094138

End: 3096219

Strand: Reverse

Name: fusA [H]

Synonym: A2cp1_2799

Alternate gene names: 220917898

Gene position: 3096219-3094138 (Counterclockwise)

Preceding gene: 220917899

Following gene: 220917897

Centisome position: 61.56

GC content: 72.43

Gene sequence:

>2082_bases
ATGGCGAACCCCCGCGTGATCCGCACCTTCTCGATGCTCGGCGCCGACGGCGCCGGGAAGACCGCGCTCGTCGAGGCGCT
GCTGCGCGTCGCCGACGCGAAGCGCGCCGCGCCCGACGGCTCGACGGCCCGCCTGGACGCCGAGCCCGAGGAGAAGAAGC
GCAACTTCACGCTGTCGCTCCACCCGGAGAGCTTCGAGGAGGACGGCCGGTCGTTCCACGTGCTCGACTGCCCCGGCTTC
GCCGCGTTCCTCACCGAGGTGGAGTGGGCGCTGCAGGTCACCGACGGCGCCATCCTGGCCATCTCCGCGGCGGACGGCGC
GCACAACCGCGCCGAGCGGACCTACGACGTGCTGGCCGAGTCCGGTCGCCCCGCGTTCGCGGTGATCACCCGGCTCGACC
ACGAGCAGGCCGACTTCCACGAGGCGCTCGAGGACATCGAGAGCTCGCTGAAGGTGAAGGCCATCCCGCTGCAGCTGCCG
GTGTTCGGGCCGGAGCGGGTCAAGGGGCTCGCCGATCTCGTCTCGATGAAGCTCCACGCCTGGGACGGGAAGGGCTTCGG
TCAGTGGACCGAGGCGGAGATCCCCGCCGAGGCGCGCGAGGAGGCGGAGCGCCTGCGCACCGCGATGGTGGAGGCGGCGG
CCGAGTCGGACGACGAGCTGCTCGGGAAGTACCTGGACGGCGCGCCGCTCGCGGAGGAGGAGATCCTGCGCGGCCTGGCC
GCCGGCGCGGCGGCGCAGCGCTTCCTGCCGGTGGCCTGCGCCGGCGCGAAGTCCGGCGTGGGCGTGCGCGAGCTGCTCGA
CCTGGCCGTGCGGATCCTGCCGGGGCCCGAGGCGCGGGCCGTGAAGGGCAGGGACCCCGCCGGCAAGGAGACCGTGCGCA
GCGCCGCGCCCGACGCGCCGTTCTCGGGCCAGGTGTTCAAGACCACCATCGACCACTTCGCCGGGCGCATCGACTACCTG
CGGGTGTTCTCCGGCACGCTCCGGGCCGACGCCACCGTGGTGAACCCGCGGACGCGCACCGAGGAGCGCATCGGCCACCT
GTACCGGACCGACGGCGCGCAGACGGTCGAGGTGAACGAGGCGGAGCCGGGCGACTTCGTGGTGCTGGTGAAGCTCAAGG
ACGCGCACACGGGCGACACGCTCTGCGATCGCGACGTGCCCCTCGCGCTGCCGCCGTTCGCGCAGCACGTGCGGCCGGTG
TCCTACGCGGTGCACGCCAAGGCCGGTGACGACAAGGCGGCCGCGGCGCTCCAGAAGATCATCGAGGAGGACCCGTCGCT
CGAGCTGACGCGCTCGACCGAGACCGGCGAGATGCTCCTCCAGGGCATGGGCCAGGCGCACCTCGACGTCACGGTGGAGC
GCGCCAAGCGCAAGCACGGCGTGGAGATCACGCTCACCCCGCCCAGCCCGGCCTACCTCGAGACGATCACCGCGGCCGCC
AAGGCGCAGGGCAAGTTCAAGCGGCAGACCGGCGGCCACGGCCAGTACGGCGACGCGCACGTCGAGCTGCAGCCCAGGGC
CCGCGGCGAGGGCTTCGAGTTCGAGGACGCGATCGTCGGCGGCGTCATCCCCCGCCAGTTCATCCCGTCGGTGGAGAAGG
GCATCCGCGGCGCGCTCTCCTCGGGCCCGCTGGCGGGCTACCCGGTGGTGGACTTCCGGGCCCGGCTGGTGTTCGGCAGC
TACCACGACGTGGACAGCTCCGACATGGCGTTCCAGGTCGCGGGCTCGATGGCGTTCAAGAAGGCGGTGCTGGAGGCCAG
GCCCATCCTGCTCGAGCCCATCATGAAGCTCGAGGTGCGGGTCCCCGAGGAGTACGTGGGCGCGGTGATGGGCGACCTGA
ACAGCCGGCGCGCCAAGGTGCAGGGCCTGGAGCCGCTGGCGCGCGGGGTGCTCATCCGCGCCACCTGCCCGCACGCCGAG
GCGATGAGCTACGACGCCGACCTGCGCTCGCTCACGCAGGGCGTCGGCTACTTCACCATGGAGCCGTCGCACCACGACCC
GGTGCCGCCGCACATCGCCCAGAAGATCATCGAGAAGCGGCGCGCGGAGGGGAAGGTGAAGGGCGTCGAGGAGGAGAAGT
AG

Upstream 100 bases:

>100_bases
TGCTAGGCCGACGCCGCCGGCGCCTCCAGGTGCCGGCTTCCGGGCAGCGAACCTCACGCCCCGCACGCTCCGGCGCGACG
GGCTGATGGAGACCGACGCC

Downstream 100 bases:

>100_bases
CGGCCGGCCGGGCGGGGGCGTCCGCGGCGCTGCGCCGGGGGGCGATCTGGACTAGGATGGCCCCTCCTTGCGCGCCTACC
TTCGCCTCTTCCGCTTCGTC

Product: small GTP-binding protein

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MANPRVIRTFSMLGADGAGKTALVEALLRVADAKRAAPDGSTARLDAEPEEKKRNFTLSLHPESFEEDGRSFHVLDCPGF
AAFLTEVEWALQVTDGAILAISAADGAHNRAERTYDVLAESGRPAFAVITRLDHEQADFHEALEDIESSLKVKAIPLQLP
VFGPERVKGLADLVSMKLHAWDGKGFGQWTEAEIPAEAREEAERLRTAMVEAAAESDDELLGKYLDGAPLAEEEILRGLA
AGAAAQRFLPVACAGAKSGVGVRELLDLAVRILPGPEARAVKGRDPAGKETVRSAAPDAPFSGQVFKTTIDHFAGRIDYL
RVFSGTLRADATVVNPRTRTEERIGHLYRTDGAQTVEVNEAEPGDFVVLVKLKDAHTGDTLCDRDVPLALPPFAQHVRPV
SYAVHAKAGDDKAAAALQKIIEEDPSLELTRSTETGEMLLQGMGQAHLDVTVERAKRKHGVEITLTPPSPAYLETITAAA
KAQGKFKRQTGGHGQYGDAHVELQPRARGEGFEFEDAIVGGVIPRQFIPSVEKGIRGALSSGPLAGYPVVDFRARLVFGS
YHDVDSSDMAFQVAGSMAFKKAVLEARPILLEPIMKLEVRVPEEYVGAVMGDLNSRRAKVQGLEPLARGVLIRATCPHAE
AMSYDADLRSLTQGVGYFTMEPSHHDPVPPHIAQKIIEKRRAEGKVKGVEEEK

Sequences:

>Translated_693_residues
MANPRVIRTFSMLGADGAGKTALVEALLRVADAKRAAPDGSTARLDAEPEEKKRNFTLSLHPESFEEDGRSFHVLDCPGF
AAFLTEVEWALQVTDGAILAISAADGAHNRAERTYDVLAESGRPAFAVITRLDHEQADFHEALEDIESSLKVKAIPLQLP
VFGPERVKGLADLVSMKLHAWDGKGFGQWTEAEIPAEAREEAERLRTAMVEAAAESDDELLGKYLDGAPLAEEEILRGLA
AGAAAQRFLPVACAGAKSGVGVRELLDLAVRILPGPEARAVKGRDPAGKETVRSAAPDAPFSGQVFKTTIDHFAGRIDYL
RVFSGTLRADATVVNPRTRTEERIGHLYRTDGAQTVEVNEAEPGDFVVLVKLKDAHTGDTLCDRDVPLALPPFAQHVRPV
SYAVHAKAGDDKAAAALQKIIEEDPSLELTRSTETGEMLLQGMGQAHLDVTVERAKRKHGVEITLTPPSPAYLETITAAA
KAQGKFKRQTGGHGQYGDAHVELQPRARGEGFEFEDAIVGGVIPRQFIPSVEKGIRGALSSGPLAGYPVVDFRARLVFGS
YHDVDSSDMAFQVAGSMAFKKAVLEARPILLEPIMKLEVRVPEEYVGAVMGDLNSRRAKVQGLEPLARGVLIRATCPHAE
AMSYDADLRSLTQGVGYFTMEPSHHDPVPPHIAQKIIEKRRAEGKVKGVEEEK
>Mature_692_residues
ANPRVIRTFSMLGADGAGKTALVEALLRVADAKRAAPDGSTARLDAEPEEKKRNFTLSLHPESFEEDGRSFHVLDCPGFA
AFLTEVEWALQVTDGAILAISAADGAHNRAERTYDVLAESGRPAFAVITRLDHEQADFHEALEDIESSLKVKAIPLQLPV
FGPERVKGLADLVSMKLHAWDGKGFGQWTEAEIPAEAREEAERLRTAMVEAAAESDDELLGKYLDGAPLAEEEILRGLAA
GAAAQRFLPVACAGAKSGVGVRELLDLAVRILPGPEARAVKGRDPAGKETVRSAAPDAPFSGQVFKTTIDHFAGRIDYLR
VFSGTLRADATVVNPRTRTEERIGHLYRTDGAQTVEVNEAEPGDFVVLVKLKDAHTGDTLCDRDVPLALPPFAQHVRPVS
YAVHAKAGDDKAAAALQKIIEEDPSLELTRSTETGEMLLQGMGQAHLDVTVERAKRKHGVEITLTPPSPAYLETITAAAK
AQGKFKRQTGGHGQYGDAHVELQPRARGEGFEFEDAIVGGVIPRQFIPSVEKGIRGALSSGPLAGYPVVDFRARLVFGSY
HDVDSSDMAFQVAGSMAFKKAVLEARPILLEPIMKLEVRVPEEYVGAVMGDLNSRRAKVQGLEPLARGVLIRATCPHAEA
MSYDADLRSLTQGVGYFTMEPSHHDPVPPHIAQKIIEKRRAEGKVKGVEEEK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=698, Percent_Identity=30.8022922636103, Blast_Score=304, Evalue=2e-82,
Organism=Homo sapiens, GI19923640, Length=732, Percent_Identity=27.1857923497268, Blast_Score=233, Evalue=4e-61,
Organism=Homo sapiens, GI25306287, Length=701, Percent_Identity=27.1041369472183, Blast_Score=200, Evalue=4e-51,
Organism=Homo sapiens, GI25306283, Length=396, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=6e-30,
Organism=Escherichia coli, GI1789738, Length=683, Percent_Identity=37.3352855051244, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1790835, Length=504, Percent_Identity=24.2063492063492, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=684, Percent_Identity=27.9239766081871, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17556745, Length=737, Percent_Identity=20.8955223880597, Blast_Score=125, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323098, Length=689, Percent_Identity=29.1727140783745, Blast_Score=306, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6322359, Length=787, Percent_Identity=22.1092757306226, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24582462, Length=702, Percent_Identity=31.0541310541311, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI221458488, Length=736, Percent_Identity=24.8641304347826, Blast_Score=192, Evalue=9e-49,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 74920; Mature: 74789

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPRVIRTFSMLGADGAGKTALVEALLRVADAKRAAPDGSTARLDAEPEEKKRNFTLSL
CCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCEEEEE
HPESFEEDGRSFHVLDCPGFAAFLTEVEWALQVTDGAILAISAADGAHNRAERTYDVLAE
CCCCHHCCCCEEEEEECCCHHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHC
SGRPAFAVITRLDHEQADFHEALEDIESSLKVKAIPLQLPVFGPERVKGLADLVSMKLHA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHEE
WDGKGFGQWTEAEIPAEAREEAERLRTAMVEAAAESDDELLGKYLDGAPLAEEEILRGLA
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHH
AGAAAQRFLPVACAGAKSGVGVRELLDLAVRILPGPEARAVKGRDPAGKETVRSAAPDAP
HHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCC
FSGQVFKTTIDHFAGRIDYLRVFSGTLRADATVVNPRTRTEERIGHLYRTDGAQTVEVNE
CCCHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEECCCCHHHHHHHHHEECCCCEEEEECC
AEPGDFVVLVKLKDAHTGDTLCDRDVPLALPPFAQHVRPVSYAVHAKAGDDKAAAALQKI
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCHHEEEEECCCCHHHHHHHHHH
IEEDPSLELTRSTETGEMLLQGMGQAHLDVTVERAKRKHGVEITLTPPSPAYLETITAAA
HHCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
KAQGKFKRQTGGHGQYGDAHVELQPRARGEGFEFEDAIVGGVIPRQFIPSVEKGIRGALS
HHCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCC
SGPLAGYPVVDFRARLVFGSYHDVDSSDMAFQVAGSMAFKKAVLEARPILLEPIMKLEVR
CCCCCCCCHHHHEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHEEE
VPEEYVGAVMGDLNSRRAKVQGLEPLARGVLIRATCPHAEAMSYDADLRSLTQGVGYFTM
CCHHHHHHHHHCCCHHHHHHCCCCHHHCCEEEEEECCCHHHHCCCHHHHHHHHCCCEEEE
EPSHHDPVPPHIAQKIIEKRRAEGKVKGVEEEK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ANPRVIRTFSMLGADGAGKTALVEALLRVADAKRAAPDGSTARLDAEPEEKKRNFTLSL
CCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCEEEEE
HPESFEEDGRSFHVLDCPGFAAFLTEVEWALQVTDGAILAISAADGAHNRAERTYDVLAE
CCCCHHCCCCEEEEEECCCHHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHC
SGRPAFAVITRLDHEQADFHEALEDIESSLKVKAIPLQLPVFGPERVKGLADLVSMKLHA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHEE
WDGKGFGQWTEAEIPAEAREEAERLRTAMVEAAAESDDELLGKYLDGAPLAEEEILRGLA
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHH
AGAAAQRFLPVACAGAKSGVGVRELLDLAVRILPGPEARAVKGRDPAGKETVRSAAPDAP
HHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCC
FSGQVFKTTIDHFAGRIDYLRVFSGTLRADATVVNPRTRTEERIGHLYRTDGAQTVEVNE
CCCHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEECCCCHHHHHHHHHEECCCCEEEEECC
AEPGDFVVLVKLKDAHTGDTLCDRDVPLALPPFAQHVRPVSYAVHAKAGDDKAAAALQKI
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCHHEEEEECCCCHHHHHHHHHH
IEEDPSLELTRSTETGEMLLQGMGQAHLDVTVERAKRKHGVEITLTPPSPAYLETITAAA
HHCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
KAQGKFKRQTGGHGQYGDAHVELQPRARGEGFEFEDAIVGGVIPRQFIPSVEKGIRGALS
HHCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCC
SGPLAGYPVVDFRARLVFGSYHDVDSSDMAFQVAGSMAFKKAVLEARPILLEPIMKLEVR
CCCCCCCCHHHHEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHEEE
VPEEYVGAVMGDLNSRRAKVQGLEPLARGVLIRATCPHAEAMSYDADLRSLTQGVGYFTM
CCHHHHHHHHHCCCHHHHHHCCCCHHHCCEEEEEECCCHHHHCCCHHHHHHHHCCCEEEE
EPSHHDPVPPHIAQKIIEKRRAEGKVKGVEEEK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]