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The map label for this gene is hisZ

Identifier: 220917791

GI number: 220917791

Start: 2970757

End: 2971806

Strand: Reverse

Name: hisZ

Synonym: A2cp1_2692

Alternate gene names: 220917791

Gene position: 2971806-2970757 (Counterclockwise)

Preceding gene: 220917792

Following gene: 220917790

Centisome position: 59.09

GC content: 79.62

Gene sequence:

>1050_bases
ATGCTCGACCTCTCGCTCCCGTCCGGCCTCCGCGACCTGCTGCCGGATCACTCGGCGCACCTGGCGGAGCTGTCCTCCAA
GCTCCACGACGTCTTCTCCCGCTTCGGCTACCGGCGCGTGTTCCTGCCCACCCTGGAGCGGCTCGACGTGGTGGAGCGGG
GGCTCTCGCCCGCGGCGCTCGCCGACGTGATGAAGTTCGTGGAGCCGGGCTCGGGCGAGGTGGTCGCGATCCGCCCGGAC
ATCACCCCGCAGATCGCCCGGCTCTACGCGGCGCGCCCGGACGCGCTGCCGTCGCCCGCGCGGCTCTGCTACGACGGCCC
GGTGCTCCGCGCGCGCGAGGCCCGGGCGGGCCGGCCGCGCGAGGTCTACCAGGCCGGCGTCGAGCTGCTGGGCGCCGGGG
GCGCCTCCGCCGACGCCGAGGCGCTGGTGCTGCTGGCGCGGTCGCTGGAGCGGGTGGGCCTGAAGGCGCCGCGCGTCGAG
GTCGGGCACGCGCGCTTCGCCGAGGCGGTCATGGAGGCGGCGCGGCTGCCGGAGCGGCTCCGGTCCGCGGCGTGGGAGGC
GCTCTCGCGCAAGGACCGCGCCGCGCTCGCCGCGGCCGCCGCGAAGGGGCGGGGGAGCGCCGAGGCGCGCGAGGCGGTGC
CGCAGCTCGCCGGCCTGTTCGGCGACGGCGCGCTCGACCGCGCGCGCGCCATCGCCCGCGCCGTGCCGGAGGCGGCGGCG
TCGCTCGCCGAGACGGAGGCGGCGCTGCGCATCGCGCGGCGTCGCGGCGTGCGCGAGGTGGCGGTGGACCTCGGCGAGGC
GCGCGGCCTCGGCTACTACACCGGCATCACGTTCGCCGGGTACGCGCCCGGCGCCGGGGCCGCGGTGGCGCGGGGCGGCC
GCTACGACGGGCTGCTCGCCCGCTTCGGTCGGCCCGGGCCGGCCATCGGCTTCGCGGTGGACCTGGAGTTCGCCACCCAG
GCGCTGGAGCGGGTGAACGGGCGGGGCCGCGGCGTCCGGCCGCGGCGCGCGTCCGCTCGCGGCGGTCGCGCGCGCGCACG
TCCGCGCTAG

Upstream 100 bases:

>100_bases
CCGCGAGCCGCGGACCGAGATCCCCGGCGCACCGCCGTCGGATCCGTACCACCACTGATCGCTTCGCAACCCGGGCGGGA
GCGAATAGAGTCGGGCGCCG

Downstream 100 bases:

>100_bases
CGGATCCGGGGCCCGGCTGGTAAGGTGTCGCACCTGCGCGTCCCGCCCGCGCCCGGTCCACGGGTGCGCGTGCGCGGCGC
GGGACGGCAGCCGTCCCGCG

Product: histidyl-tRNA synthetase 2

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLDLSLPSGLRDLLPDHSAHLAELSSKLHDVFSRFGYRRVFLPTLERLDVVERGLSPAALADVMKFVEPGSGEVVAIRPD
ITPQIARLYAARPDALPSPARLCYDGPVLRAREARAGRPREVYQAGVELLGAGGASADAEALVLLARSLERVGLKAPRVE
VGHARFAEAVMEAARLPERLRSAAWEALSRKDRAALAAAAAKGRGSAEAREAVPQLAGLFGDGALDRARAIARAVPEAAA
SLAETEAALRIARRRGVREVAVDLGEARGLGYYTGITFAGYAPGAGAAVARGGRYDGLLARFGRPGPAIGFAVDLEFATQ
ALERVNGRGRGVRPRRASARGGRARARPR

Sequences:

>Translated_349_residues
MLDLSLPSGLRDLLPDHSAHLAELSSKLHDVFSRFGYRRVFLPTLERLDVVERGLSPAALADVMKFVEPGSGEVVAIRPD
ITPQIARLYAARPDALPSPARLCYDGPVLRAREARAGRPREVYQAGVELLGAGGASADAEALVLLARSLERVGLKAPRVE
VGHARFAEAVMEAARLPERLRSAAWEALSRKDRAALAAAAAKGRGSAEAREAVPQLAGLFGDGALDRARAIARAVPEAAA
SLAETEAALRIARRRGVREVAVDLGEARGLGYYTGITFAGYAPGAGAAVARGGRYDGLLARFGRPGPAIGFAVDLEFATQ
ALERVNGRGRGVRPRRASARGGRARARPR
>Mature_349_residues
MLDLSLPSGLRDLLPDHSAHLAELSSKLHDVFSRFGYRRVFLPTLERLDVVERGLSPAALADVMKFVEPGSGEVVAIRPD
ITPQIARLYAARPDALPSPARLCYDGPVLRAREARAGRPREVYQAGVELLGAGGASADAEALVLLARSLERVGLKAPRVE
VGHARFAEAVMEAARLPERLRSAAWEALSRKDRAALAAAAAKGRGSAEAREAVPQLAGLFGDGALDRARAIARAVPEAAA
SLAETEAALRIARRRGVREVAVDLGEARGLGYYTGITFAGYAPGAGAAVARGGRYDGLLARFGRPGPAIGFAVDLEFATQ
ALERVNGRGRGVRPRRASARGGRARARPR

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Escherichia coli, GI1788861, Length=333, Percent_Identity=26.7267267267267, Blast_Score=82, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24643061, Length=373, Percent_Identity=24.3967828418231, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24643059, Length=389, Percent_Identity=24.1645244215938, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_ANAD2 (B8JDI0)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493095.1
- ProteinModelPortal:   B8JDI0
- SMR:   B8JDI0
- GeneID:   7299693
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2692
- HOGENOM:   HBG616575
- ProtClustDB:   CLSK945558
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 36967; Mature: 36967

Theoretical pI: Translated: 11.16; Mature: 11.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLSLPSGLRDLLPDHSAHLAELSSKLHDVFSRFGYRRVFLPTLERLDVVERGLSPAAL
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
ADVMKFVEPGSGEVVAIRPDITPQIARLYAARPDALPSPARLCYDGPVLRAREARAGRPR
HHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHCCCCCH
EVYQAGVELLGAGGASADAEALVLLARSLERVGLKAPRVEVGHARFAEAVMEAARLPERL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RSAAWEALSRKDRAALAAAAAKGRGSAEAREAVPQLAGLFGDGALDRARAIARAVPEAAA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLAETEAALRIARRRGVREVAVDLGEARGLGYYTGITFAGYAPGAGAAVARGGRYDGLLA
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEECCCEECCCCCCCCHHHHCCCCHHHHHH
RFGRPGPAIGFAVDLEFATQALERVNGRGRGVRPRRASARGGRARARPR
HCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDLSLPSGLRDLLPDHSAHLAELSSKLHDVFSRFGYRRVFLPTLERLDVVERGLSPAAL
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
ADVMKFVEPGSGEVVAIRPDITPQIARLYAARPDALPSPARLCYDGPVLRAREARAGRPR
HHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHCCCCCH
EVYQAGVELLGAGGASADAEALVLLARSLERVGLKAPRVEVGHARFAEAVMEAARLPERL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RSAAWEALSRKDRAALAAAAAKGRGSAEAREAVPQLAGLFGDGALDRARAIARAVPEAAA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLAETEAALRIARRRGVREVAVDLGEARGLGYYTGITFAGYAPGAGAAVARGGRYDGLLA
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEECCCEECCCCCCCCHHHHCCCCHHHHHH
RFGRPGPAIGFAVDLEFATQALERVNGRGRGVRPRRASARGGRARARPR
HCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA