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The map label for this gene is purA

Identifier: 220917790

GI number: 220917790

Start: 2969325

End: 2970623

Strand: Reverse

Name: purA

Synonym: A2cp1_2691

Alternate gene names: 220917790

Gene position: 2970623-2969325 (Counterclockwise)

Preceding gene: 220917791

Following gene: 220917789

Centisome position: 59.07

GC content: 71.82

Gene sequence:

>1299_bases
ATGCCGAACGTGGTCGTCGTCGGAGCCCAGTGGGGCGACGAGGGCAAGGGCAAGATCGTCGATCTGCTGACCCAGTACGC
CGACGTGGTGGTGCGGTTCCAGGGCGGCAACAACGCCGGCCACACGCTGGTGGTCGGGGGCGAGAAGACCGTCCTCCACC
TCATCCCGTCCGGCATCCTCCACCCGGGGAAGTCGTGCGTCATCGGGAACGGCGTGGTGATCGACCCCGAGGTGCTGGTC
CTCGAGATCGACCGGCTGAAGGCGAAGGGCGCGCTGAAGGACGACGGCCAGCTCGTGGTCTCGCTGGACGCCCACGTGAT
CATGCCGTGGCACAAGGCCATCGACGTGGCGCGCGAGCAGGCCATGGGCGAGGGCAAGATCGGCACCACCGGCCGCGGCA
TCGGGCCGACCTACGAGGACAAGGTGGCGCGGCGCGGGCTGCGCATCCGCGACCTGCTCGACGAGGCGCGCCTCGCCCGC
AAGGTGAAGGAGCGCGCCGCGCTCGCCCGCGAGGAGCTGGCGCGCCTCGGCGCGAAGCTCGAGCTCGACGAGCCGGCGCT
GGTGAAGCGCTACGCCGAGCTGGGCCGCCGCGTCGCCGGCTACGCCACCGACGTGTCGATCTGGCTGCACCGCGCGCTGC
AGCAGGGCAAGTCGCTCCTGTTCGAGGGCGCGCAGGGCACCATGCTCGACGTGGACCACGGCACGTACCCGTTCGTCACC
AGCTCGAACACGGTGGCCGGGAACGCGGTGGTCGGGTGCGGCCTCGGGCCGACCGCGGTGGACTACGTGCTCGGCATCTC
CAAGGCCTACTCCACGCGCGTGGGCGGCGGCCCGTACCCCACCGAGCTGAAGGACGAGACCGGCGAGCGGCTGCGCAAGC
TCGGCGGCGAGTACGGCGCCACCACCGGCCGCCCGCGCCGCACCGGCTGGCTGGACGCGCTCGCGCTCCGCTACGCGGTG
CGGGTGAACGGGCTCTCCGGCATCGCCATGACGAAGCTCGACGTCCTCACCGGCTTCGACACGGTGAAGATCGCGGTCGG
GTACCGGCTCGACGGCAAGGTCCTCGACGAGATGCCGAGCGACCCGGAGGTCATCGAGCGCTGCACGCCGGTGTACGAGG
AGCTGCCCGGCTGGACCGAGAAGCTCGAGCACCTGCGCACCTGGGACGACCTGCCGCCGCGCGCCCGCGCCTACGTGAAG
CGGGTCGAGGAGCTCGCCGGCGTGAAGGTCGTCGGCTGCTCCGTCGGCGCCGACCGCGGCGAGACCATCCTGGTCGAGAA
CCCGTTCCTGGCGCGCTGA

Upstream 100 bases:

>100_bases
CTGCGCGTCCCGCCCGCGCCCGGTCCACGGGTGCGCGTGCGCGGCGCGGGACGGCAGCCGTCCCGCGTCCGGGTGGGCCC
GCCGACAGGGAGAGCCAAAC

Downstream 100 bases:

>100_bases
TCGCCGCTCGCAGGGGGGCGCCCCGGCTCCGGCCGCGGGCGCCCGTGCCGGCCCGCATGCGCCGTCCCGTCCTCCTCCTC
TGGCTGGCCGTGCTCGTCGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPNVVVVGAQWGDEGKGKIVDLLTQYADVVVRFQGGNNAGHTLVVGGEKTVLHLIPSGILHPGKSCVIGNGVVIDPEVLV
LEIDRLKAKGALKDDGQLVVSLDAHVIMPWHKAIDVAREQAMGEGKIGTTGRGIGPTYEDKVARRGLRIRDLLDEARLAR
KVKERAALAREELARLGAKLELDEPALVKRYAELGRRVAGYATDVSIWLHRALQQGKSLLFEGAQGTMLDVDHGTYPFVT
SSNTVAGNAVVGCGLGPTAVDYVLGISKAYSTRVGGGPYPTELKDETGERLRKLGGEYGATTGRPRRTGWLDALALRYAV
RVNGLSGIAMTKLDVLTGFDTVKIAVGYRLDGKVLDEMPSDPEVIERCTPVYEELPGWTEKLEHLRTWDDLPPRARAYVK
RVEELAGVKVVGCSVGADRGETILVENPFLAR

Sequences:

>Translated_432_residues
MPNVVVVGAQWGDEGKGKIVDLLTQYADVVVRFQGGNNAGHTLVVGGEKTVLHLIPSGILHPGKSCVIGNGVVIDPEVLV
LEIDRLKAKGALKDDGQLVVSLDAHVIMPWHKAIDVAREQAMGEGKIGTTGRGIGPTYEDKVARRGLRIRDLLDEARLAR
KVKERAALAREELARLGAKLELDEPALVKRYAELGRRVAGYATDVSIWLHRALQQGKSLLFEGAQGTMLDVDHGTYPFVT
SSNTVAGNAVVGCGLGPTAVDYVLGISKAYSTRVGGGPYPTELKDETGERLRKLGGEYGATTGRPRRTGWLDALALRYAV
RVNGLSGIAMTKLDVLTGFDTVKIAVGYRLDGKVLDEMPSDPEVIERCTPVYEELPGWTEKLEHLRTWDDLPPRARAYVK
RVEELAGVKVVGCSVGADRGETILVENPFLAR
>Mature_431_residues
PNVVVVGAQWGDEGKGKIVDLLTQYADVVVRFQGGNNAGHTLVVGGEKTVLHLIPSGILHPGKSCVIGNGVVIDPEVLVL
EIDRLKAKGALKDDGQLVVSLDAHVIMPWHKAIDVAREQAMGEGKIGTTGRGIGPTYEDKVARRGLRIRDLLDEARLARK
VKERAALAREELARLGAKLELDEPALVKRYAELGRRVAGYATDVSIWLHRALQQGKSLLFEGAQGTMLDVDHGTYPFVTS
SNTVAGNAVVGCGLGPTAVDYVLGISKAYSTRVGGGPYPTELKDETGERLRKLGGEYGATTGRPRRTGWLDALALRYAVR
VNGLSGIAMTKLDVLTGFDTVKIAVGYRLDGKVLDEMPSDPEVIERCTPVYEELPGWTEKLEHLRTWDDLPPRARAYVKR
VEELAGVKVVGCSVGADRGETILVENPFLAR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=44, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.3529411764706, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=42.0253164556962, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=53.0232558139535, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=42.8240740740741, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=42.8240740740741, Blast_Score=335, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=41.92037470726, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=41.8439716312057, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ANAD2 (B8JDH9)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493094.1
- ProteinModelPortal:   B8JDH9
- SMR:   B8JDH9
- GeneID:   7299692
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2691
- HOGENOM:   HBG658237
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46695; Mature: 46564

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNVVVVGAQWGDEGKGKIVDLLTQYADVVVRFQGGNNAGHTLVVGGEKTVLHLIPSGIL
CCCEEEEECCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEEECCCCCC
HPGKSCVIGNGVVIDPEVLVLEIDRLKAKGALKDDGQLVVSLDAHVIMPWHKAIDVAREQ
CCCCCEEECCCEEECCCEEEEEEEHHHCCCCCCCCCCEEEEECCEEEECHHHHHHHHHHH
AMGEGKIGTTGRGIGPTYEDKVARRGLRIRDLLDEARLARKVKERAALAREELARLGAKL
HCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELDEPALVKRYAELGRRVAGYATDVSIWLHRALQQGKSLLFEGAQGTMLDVDHGTYPFVT
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCHHHEEECCCCCEEECCCCCCCEEE
SSNTVAGNAVVGCGLGPTAVDYVLGISKAYSTRVGGGPYPTELKDETGERLRKLGGEYGA
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
TTGRPRRTGWLDALALRYAVRVNGLSGIAMTKLDVLTGFDTVKIAVGYRLDGKVLDEMPS
CCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCC
DPEVIERCTPVYEELPGWTEKLEHLRTWDDLPPRARAYVKRVEELAGVKVVGCSVGADRG
CHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
ETILVENPFLAR
CEEEECCCCCCC
>Mature Secondary Structure 
PNVVVVGAQWGDEGKGKIVDLLTQYADVVVRFQGGNNAGHTLVVGGEKTVLHLIPSGIL
CCEEEEECCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEEECCCCCC
HPGKSCVIGNGVVIDPEVLVLEIDRLKAKGALKDDGQLVVSLDAHVIMPWHKAIDVAREQ
CCCCCEEECCCEEECCCEEEEEEEHHHCCCCCCCCCCEEEEECCEEEECHHHHHHHHHHH
AMGEGKIGTTGRGIGPTYEDKVARRGLRIRDLLDEARLARKVKERAALAREELARLGAKL
HCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELDEPALVKRYAELGRRVAGYATDVSIWLHRALQQGKSLLFEGAQGTMLDVDHGTYPFVT
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCHHHEEECCCCCEEECCCCCCCEEE
SSNTVAGNAVVGCGLGPTAVDYVLGISKAYSTRVGGGPYPTELKDETGERLRKLGGEYGA
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
TTGRPRRTGWLDALALRYAVRVNGLSGIAMTKLDVLTGFDTVKIAVGYRLDGKVLDEMPS
CCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCC
DPEVIERCTPVYEELPGWTEKLEHLRTWDDLPPRARAYVKRVEELAGVKVVGCSVGADRG
CHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
ETILVENPFLAR
CEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA